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精子 洗滌液의 pH와 洗滌頻度 및 수소個體가 精子洗滌液內 水素이온濃度와 소 精子의 尖帽反應에 미치는 影響
박영식,임경순,Park, Yeong-Sik,Im, Gyeong-Sun 한국동물번식학회 1991 Reproductive & developmental biology Vol.15 No.1
This study was carried out to investigate the effects of pH of sperm-washing solution, sperm-washing frequency and individual bull on concentration of hydrogen ion in sperm-washed solution and sperm acrosome reaction. The results obtained were as follow. 1. When bovine sperm was washed at 4 times with SHP solution and incubated, the difference of light absorbance between sperm-washing and sperm-washed solution(${\Delta}$Ao-${\Delta}$At) was higher in 2nd sperm-washed solution than that in the other washed solutions. 2. When sperm was thrice washed with SHP solutions of pH 5.99, 6.38, 6.78, 7.10, 7.40, 7.69, 8.15, 8.45, and 8.83, ${\Delta}$Ao-${\Delta}$At was significantly increased at pH7.69 to 8.83, and ${\Delta}$Ao-${\Delta}$At in 1st sperm washed solution was significantly higher than that in 2nd and 3rd sperm washed solution. 3. When sperm of Holstein, KNC and Hereford was thrice washed with SHP solutions of pH 5.99, 6.38, 6.78, 7.10, 7.40, 7.69, 8.15, 8.45, and 8.83, Holstein showed higher ${\Delta}$Ao-${\Delta}$At of sperm Washed solution than KNC and Hereford, and ${\Delta}$Ao-${\Delta}$At in sperm washed solution was significantly increased at 7.69 for Holstein and at 8.15 for KNC and Hereford, respectively. 4. When sperm was thrice with SHP solution of pH 6.8, 7.1 and 7.4, and then incubated in mTALP of pH 7.4 for 15 minutes, in 1st and 2nd sperm washing ${\Delta}$Ao-${\Delta}$At of sperm washed solution was significantly higher at pH 7.1 and 7.4 than at pH 6.8, and the sperm acrosome reaction of pH 6.8, 7.1 and 7.4 was 49.1, 68.8 and 72.9%, respectively. The sperm acrosome reaction of pH 7.1 and 7.4 was higher than that of pH 6.8.
영역기반 영상부호화를 위한 이진 분열에 의한 계층적인 영상분할
박영식,송근원,한규필,이호영,남재열,하영호,Park, Young-Sik,Song, Kun-Woen,Han, Kyu-Phil,Lee, Ho-Young,Nam, Jae-Yeal,Ha, Yeong-Ho 대한전자공학회 1998 電子工學會論文誌, S Vol.s35 No.8
본 논문에서는 이진 분열(binary split)에 의한 새로운 계층구조(hierarchical structure)의 형태학적 영상분할 알고리즘을 제안하였다. 이는 각 계층에서 화질이 가장 낮은 하나의 영역에서 생성된 여러 마커중에서 단지 밝기 차가 가장 큰 두 개의 마커를 이용하여 두 영역으로 분할한다. 따라서 제안한 알고리즘은 기존의 방법보다 제한된 개수의 영역으로 화질의 개선을 가져오고 부호화 비용이 많이 소요되고, 인간시각에 민감하지 않는 경계선 정보를 감소시킨다. 또한 분할되는 영역의 개수를 조절할 수 있어 대역폭이 매우 제한된 PSTN(public switched telephone network), LAN, 그리고 이동통신망과 같은 환경에서 사용하기에 적합하다. 그리고 분할 결과에서 작은 영역을 없애기 위한 후처리 과정이 필요 없고, 각 계층의 단순화 단계에서 작은 크기의 형태소만을 사용하므로 기존의 방법보다 연산량이 적고 구조적으로 매우 간단하다. In this paper, a new morphological image segmentation algorithm of hierarchical structure by binary split is proposed. It splits a region with the lowest quality into two regions using only two markers having the highest contrast. Therefore, it improves the quality of image with limited regions and reduces contour information which is not sensitive to human visual system, when compared with the conventional algorithm. It is appropriate to PSTN, LAN, and mobile networks, of which the available transmission bandwidth is very limited, because the number of regions can be controlled. And the proposed algorithm shows very simple structure because it doesn't need post processing to eliminate small regions and reduces much computation by using only structuring element of small size at simplification step of each hierarchical structure when compared with the conventional algorithm.