Molecular Typing of Leuconostoc citreum Strains Isolated from Korean Fermented Foods Using a Random Amplified Polymorphic DNA Marker

박영서,이설희,Anshul Sharma,박영서 한국산업식품공학회 2017 산업 식품공학 Vol.20 No.2

For preliminary molecular typing, PCR-based fingerprinting using random amplified polymorphic DNA (RAPD) is the method of choice. In this study, 14 bacterial strains were isolated from different Korean food sources, identified using 16S rRNA gene sequencing, and characterized through RAPD-PCR. Two PCR primers (239 and KAY3) generated a total of 130 RAPD bands, 14 distinct PCR profiles, 10 polymorphic bands, one monomorphic band, and four unique bands. Dendrogram-based analysis with primer 239 showed that all 14 strains could be divided into seven clades out of which clade VII had the maximum of seven. In contrast, dendrogram analysis with the primer KAY3 divided the 14 L. citreum strains into four clades out of which clade IV consisted of a maximum of 10 strains out of 14. This research identified and characterized bacterial populations associated with different Korean foods. The proposed RAPD-PCR method, based on sequence amplification, could easily identify and discriminate the lactic acid bacteria species at the strain-specific level and could be used as a highly reliable genomic fingerprinting tool.

발전하는 학회의 위상을 뒤돌아보며

박영서 한국산업식품공학회 2023 식품과 기계 Vol.20 No.1

Furosemide의 이뇨작용과 신조직내 Cyclic Nucleotide 함량에 미치는 Hydrocoritisone의 영향

박영서 가톨릭대학 (가톨릭신학대학) 대학원 1983 가톨릭大學醫學部 論文集 Vol.36 No.2

양측성 요관신우부의 진균구에 의한 무뇨증을 보인 영아

박영서,이정주,김건석,윤종현 대한신장학회 1998 Kidney Research and Clinical Practice Vol.17 No.5

Fungal infection has been observed with increasing frequency in recent years because the use of combinations of broad spectrum antibiotics, immunosuppressive agents, and antineoplastic agents is increasing and the survival rate of premature baby is increasing. We experienced a 3 month old male infant with anuria due to bilateral ureteropelvic fungus balls. He was born at 31 weeks gestation period and had been treated with broad spectrum antibiotics for 5 weeks after birth. We removed fungus balls surgically and made nephrostomy bilaterally. And then irrigation of amphotericn B through nephrostomy and systemic amphotercin B injection had performed for 3 weeks. Thereafter fungus balls completely disappeared.

Bacillus alcalophilus AX2000 유래 xylanase 유전자 (XynT)의 Cloning과 염기서열 분석

박영서,Park Young-Seo 한국생명과학회 2005 생명과학회지 Vol.15 No.5

Xylanase를 생산하는 알칼리 내성 Bacillus alcalophilus AX2000의 chromosomal DNA로부터 xylanase 유전자를 cloning하여 그 염기배열 순서를 결정한 다음 이로부터 유전자 발현에 관련된 구조를 분석하였다. Xylanase 유전자의 cloning을 위해 제한효소 PstI으로 절단한 B. alcalophilus AX2000의 chromosomal DNA와 pUC19을 ligation 시켜 E. coli $DH5\alpha$에 형질전환시킨 후 형질전환체 중에서 xylanase 활성을 나타내는 재조합 plasmid pXTY99를 분리하였다. 재조합 plasmid pXTY99은 pUC19의 PstI 부위 내에 7kb의 외래 DNA가 삽입 되 었다. Cloning된 xylanase 유전자(xynT)의 염기배열을 분석한 결과 유전자의 크기는 1,020 bp이었고 이는 340개의 아미노산으로 구성된 분자량 40 kDa의 poly-peptide를 coding하고 있었다. 이 염기배열은 AUG 개시 codon으로부터 각각 259와 282 base상류에 TACAAT의 -10 box와 GTTCACA인 -35 box로 추정되는 염기배열이 존재하였으며 ribosome 결합부위가 존재하였다. B. alcalophilus AX2000의 xylanase와 아미노산배열의 유사성이 가장 높은 xylanase는 Bacillus sp. N137과 B. stearothemophilus 21 유래의 xylanase로 각각 $61\%$와 $59\%$의 유사성을 나타내었다. A gene coding for xylanase from alkali-tolerant Bacillus alcalophilus AX2000 was cloned into Escherichia coli $DH5\alpha$ using pUC19. Among 2,000 transformants, one transformant showed clear zone on the detection agar plate containing oat-spells xylan. Its recombinant plasmid, named pXTY99, was found to carry 7.0 kb insert DNA fragment. When the nucleotide sequence of the cloned xylanase gene (xynT) was determined, xynT gene was found to consist of 1,020 base-pair open reading frame coding for a poly-peptide of 340 amino acids with a deduced molecular weight of 40 kDa. The coding sequence was preceded by a putative ribosome binding site, and the transcription initiation signals. The deduced amino acid sequence of xylanase is similar to those of the xylanases from Bacillus sp. Nl37 and B. stearothermophilus 21 with $61\%$ and $59\%$ identical residues, respectively.

식품 산업발전을 위한 학회의 역할

박영서 한국식품과학회 2022 식품과학과 산업 Vol.55 No.2

稅務會計上의 所得金額計算에 관한 實證的 考察 : Especially on income standard ratio

朴永緖 연세대학교 경영대학원 1968 經營論叢 Vol.2 No.4

4Income tax has the different notion from the income on enterprise accounting. Namely, though the net profit(income) gained from enterprise management results can be computed by enterprise accounting principal that could be recognized as just and proper one, assessment standard which is to impose the taxable incomes on tax accounting is made by the different accounting because it is the net profit considered by the particularity of enterprise itself. Tax law is to impose for the tax purpose aiming to levy system of enterprise in principal, but it is understood that the Government could consider and decide the income of each enterprise concerned in order to impose tax, if there is tremendous difference by neglecting the income report of enterprise and by adjusting the incomes. Because management and administration of enterprise can be appeared as many varieties by the operators' subjectivity or enterprise conventional particularity, the enterprise income are much different each other because of enterprise policy and account disposition even the business items, scales and kinds are same during the same period operation. Therefore, if the enterprise accounting is computed in consideration of principal and standard of tax accounting, limitation of levy tax has no special difference in the end. For example, if it is very diffieult or impossible to compute the enterprise incomes because of no vouchers and ledgers or unfaithful record of the business transaction, the Government should take inevitably action to consider and decide the enterprise incomes for tax imposition, because we know that the final purpose of modern enterprise is to seek out profits, and also, if there is any regular economic action, some incomes is naturally accompanied, and if there is incomes, there should be imposed the taxes. Now here are problems that how the Government will decide and impose the taxes of the real incomes of enterprise with what a standard and means, and for the purpose of sufficient taxation process under realization of social justice, how the public and just imposition could be taken. Concerning the tax accounting (tax law) the followings are considered as ways of investigation and decision. 1. In accordance with the authority of other same traders of the same kind business 2. Through applying for income standard ratio according to each business classification, which are investigated by the Government, it is to decide taxable income. The above item one is rarely utilized to decide the amount of income, for it cannot help considering the income contents of the other same kinds of the same business items, and so, we can say that this case has not worth of practical use because of deficiency of universality on tax accounting. Thus, the standard points of tax imposing computation regardless juridical person and private business is the income standard ratio. If we look into the existing taxation examples, we find out that about 39.4% (among the present operating 61799 enterprises) and 99.8% (among the individual business of 249,067) have been considered and computed by the administration tax rate as the income standard ratio. Because this income standard ratio is the standard value of income tax, it is very interesting value on tax allotment of enterprise and imposition of the Government sufficient taxation purpose. Thus it comes to a conclusion that the resolution problem of standard ratio should be just and rational both nominally and virtually. This income standard ratio has not only the function of computation of taxable income but also the standard to examine relatively the practical income of enterprise. Sometimes, it involves the cause of examing subject of taxes affairs and it becomes also a utilizing factors to introduce the enterprise accounting into the settlement on policy. And so, this has the limitatation to obstruct and fabricate the realization of practical income amounts. Therefore, the main purpose to study of the applying settlement of income standard ratio as the 2nd taxes rate is to research about practical points of letting try to approach the taxes accounts and enterprise accounting each other, referring the importance of income standard ratio as tool of estimating taxable income. The contents of this subject is the following. Ⅰ. Introduction Ⅱ. Estimated Assessment on Taxable Income A. Significance of taxable income B. Assesement standard and method on tax account C. Assessment method in foreign countries D. Estimation on tax account E. Resolution method of income tax Estimated determination method of taxable income Ⅲ. Income standard ratio as income estimating means A. Outline of income standard ratio B. Determining Method of income standard ratio in Korea C. Difference of income standard ratio between Korea and foreign country(especially Japan) Ⅳ. Rationalization problem of income standard ratio A. Determination of proper income standard ratio B. Opening to public or not about income standard ratio Ⅴ. Improvement Plan A. Basic course of improvement of income standard ratio B. Improvement method for rationalization of income standard ratio Ⅵ. Prediction from improvement of income standard ratio Ⅶ. Conclusion

신생아 요관신우 이행부 폐쇄의 진단에 있어 Tc - 99m MAG3 이뇨제 신스캔의 유용성

박영서,김건석,이희경,이소영,정동진,강도영,문대혁 대한핵의학회 1997 핵의학 분자영상 Vol.31 No.4

Bacillus Pumilus TX703 유래 Xylanase의 활성에 관여하는 아미노산 잔기의 확인

박영서,Park Young-Seo 한국생명과학회 2005 생명과학회지 Vol.15 No.4

The purified xylanase from Bacillus pumilus TX703 was modified with various chemical modifiers to determine the active sites of the enzyme. Treatment of the enzyme with group-specific reagents such as carbodiimide or N-bromosuccinimide resulted in complete loss of enzyme activity. These results assumed that these reagents reacted with glutamic acid or aspartic acid and tryptophan residues located at or near the active site. In each case, inactivation was performed by pseudo first-order kinetics. Inhibition of enzyme activity by carbodiimide and W-bromosuccinimide showed non-competitive and competitive inhibition type, respectively. Addition of xylan to the enzyme solution containing N-bromosuccinimide prevented the inactivation, indicating the presence of tryptophan at the substrate binding site. Analysis of kinetics for inactivation showed that the loss of enzyme activity was due to modification of two glutamic acid or aspartic acid residues and single tryptophan residue. Bacillus pumilus TX703으로부터 xylanase를 정제한 후 효소의 활성부위를 조사하기 위하여 여러 가지 화학수식제를 사용하여 효소활성의 저해도를 측정하였다. 여러 가지 화학수식제 중에서 carbodiimide와 N-bromosuccinimide가 효소활성을 완전히 저 해시 켜 glutamic acid 또는 aspartic acid 잔기와 tryptophan 잔기가 효소의 활성부위에 관여하리라 추측되었다. 각각의 경우에 효소 실활은 수식제의 첨가농도에 따라 pseudo first-order kinetics 양식을 보여주었으며, car-bodiimide와 N-bromosuccinimide는 각각 비경쟁적 저해와 경쟁적 저해방식을 나타내었다. 기질첨가에 의한 효소활성 보호실험을 통하여 tryptophan 잔기가 기질결합부위라 판단되었다. 효소실활속도의 분석에 의해 효소활성에는 2개의 glutamic acid 또는 aspartic acid 잔기와 1개의 tryptophan 잔기가 관여하는 것으로 나타났다.

珪肺의 病理

朴映緖 가톨릭대학산업의학센타 산업의학연구소 1964 韓國의 産業醫學 Vol.3 No.3

규폐는 금광, 탄광, 석공장 등에서 세균과도 같이 적은 규소미립자가 많이 함유되어 있는 분진을 장기간 흡입하며 작업하는 자들에서, 주로 폐 및 폐기관지 임파계통에 발생하는 육아종성염증질환이다. 규폐가 발생되는 데는 두 가지 요인이 있으며, 흡입되는 분진에 함유되어 있는 규소의 농도와 작업기간이다.