삼세기 Hemitripterus villosus의 산란생태, 난발생 및 자치어의 형태발달

박애전 ( Ae Jeon Park ),한경호 ( Kyeong Ho Han ),이성훈 ( Sung Hoon Lee ),김희진 ( Hui Jin Kim ),김승용 ( Seung Yong Kim ),임인현 ( In Hyeon Lim ) 한국어류학회 2014 韓國魚類學會誌 Vol.26 No.1

The present study describes the spawning ecology and early morphological development of Hemitripterus villosus. The natural spawning ground consisted of bedrock and pebbles was the intertidal coast at Taean (Chungnam) and its depth was about 5~~10 m. Spawning period was mainly from the end of October to December, when the water temperature and salinity were 6.0~~ 15.8℃ and mean 32.0‰, respectively. There were no difference of the body shape and color between female and male of Hemitripterus villosus, however its reproductive organs showed clear differences. The male had tube shaped genital papilla, which was connected with testis, and the female had seminal recepacle, which was the lower part of oviduct connected with ovary. Genital papilla of male came out of its body at spawning period and then male copulated. After copulation, female stored the sperm in its seminal recepacle and fertilized when it spawned. Fertilized eggs were reached 8 cells stage after fertilization at rearing water temperature 8.2~~14.9℃. At 29 hours after fertilization, it reached morula stage, and at 146 hours after fertilization, its embryo was clearly formated. Hatching was begun from 1,488 hours (62 days) after fertilization with 8.2~~14.9℃ water temperature. The newly hatched larvae were 12.99~~15.46mm(mean 14.16±0.65 mm) in TL (Total Length), and its mouth and anus were open. At 7 days after hatching, its yolk sac was completely absorbed and the myotomes were 15+25=40, measuring 15.23~~15.54mm(mean 15.39±0.22 mm, n=5) in TL. At 75~~80 days after hatching, it was measured mean 30.06±0.76 mm in TL, and it had reached the juvenile stage with the complete set of fin rays.

수하시기에 따른 참굴 인공종묘의 성장 비교

이정미,박애전,조상만,박경대 한국패류학회 2008 The Korean Journal of Malacology Vol.24 No.2

In order to investigate the possibility of yearly-harvest, we carried out growth experiment (shell height, meat weight and condition index) for of the artificial oyster spat, which suspended in three different times before the season for natural spat around coastal waters of Gyeongnam Province. Shell growth of spats suspended in June showed higher than those in April and May. In meat weight, the growth of spats was significantly elevated from October, of which significant difference was observed depending on growing farms (Gosung<Tongyeong and Koje). Using condition index, we found that the spat deployed in April and May were spawned on August or September, however, could not that in June. The estimated yield time were accounted for September on the shell height level (≥ 80 mm) and October on the meat weight level (≥ 5 g). In order to investigate the possibility of yearly-harvest, we carried out growth experiment (shell height, meat weight and condition index) for of the artificial oyster spat, which suspended in three different times before the season for natural spat around coastal waters of Gyeongnam Province. Shell growth of spats suspended in June showed higher than those in April and May. In meat weight, the growth of spats was significantly elevated from October, of which significant difference was observed depending on growing farms (Gosung<Tongyeong and Koje). Using condition index, we found that the spat deployed in April and May were spawned on August or September, however, could not that in June. The estimated yield time were accounted for September on the shell height level (≥ 80 mm) and October on the meat weight level (≥ 5 g).

자주복 , Takifugu rubripes 자치어의 형태

김용억,박애전 한국어류학회 1990 韓國魚類學會誌 Vol.2 No.2

여수 연안산 삼세기 (Hemitripterus villosus) 자치어의 골격발달

전나영 ( Na-young Jeon ),박애전 ( Ae-jeon Park ),이성훈 ( Sung-hoon Lee ),유태식 ( Tae-sik Yu ),한경호 ( Kyeong-ho Han ) 한국어류학회 2024 韓國魚類學會誌 Vol.36 No.1

삼세기 (Hemitripterus villosus)는 새로운 양식 어종으로 대두되고 있으나, 자원량이 감소하고 있으며, 양식 어종에서 지속적인 문제로 나타나는 골격 기형에 대해 삼세기의 정상적인 골격발달 기준을 제시하고자 한다. 이 연구에 사용된 시료는 어획된 어미로부터 채란된 수정란을 부화시킨 자치어를 포르말린액에 고정한 뒤 Kawamura and Hosoya (1991) 염색법에 따라 염색하여 골격발달을 관찰하였다. 부화 직후 평균전장 13.65±0.71mm(n=5) 일 때 부설골과 주상악골, 전상악골, 치골이 골화되기 시작하였고, 부화 후 65일째 평균전장 21.24±0.50mm(n=5) 일 때 사골과 안상골이 골화하면서 두부가 완성되었다. 견대부는 부화 7일째 평균전장 14.61±0.52 mm (n=5) 일 때 쇄골, 주새개골, 관절골이 골화하기 시작하였으며, 부화 44일째 평균전장 18.15±0.61mm(n=5) 일 때, 4개의 사출골이 골화되어 견대부가 완성되었다. 부화 10일째 평균 전장 14.80±0.65mm(n=5) 일 때 척추골의 골화가 시작되었으며, 부화 후 54일째 평균전장 18.67±0.54 mm (n=5) 일 때 척추골 추체 39개가 골화하여, 부화 60일째 평균전장 20.25±0.45mm(n=5) 일 때 척추골의 골격이 완성되었다. 이 연구는 삼세기 자치어의 골격발달에 대한 것으로 삼세기의 자치어 골격 기형의 기준과 기초자료 연구에 사용될 것이다. Hemitripterus villosus, a promising aquaculture fish species, is facing declining stocks. This study aims to provide normative standards for skeletal development to address persistent skeletal deformities in farmed fish. Specimens utilized in the study underwent artificial insemination with captured fish, and the resulting larvae and fry were preserved in a formalin solution. The skeletal ossification process commenced immediately after hatching, affecting the parasphenoid, premaxillary, maxillary, and dentary structures at an average total length of 13.65±0.71 mm (n=5). By sixty-five days post-hatching, ossification extended to the ethmoid and supraorbital, completing the head’s development at an average total length of 21.24±0.50 mm (n=5). Clavicle ossification occurred at seven days post-hatching, corresponding to an average total length of 14.61±0.52 mm (n=5). At forty-four days post-hatching, the ossification of 4 actinosts took place, completing the shoulder girdle, with an average total length of 18.15±0.61 mm (n=5). Vertebral ossification initiated at ten days post-hatching, with an average total length of 14.80±0.65 mm (n=5). By fifty-four days post-hatching, 39 vertebral columns were ossified, reaching an average total length of 18.67±0.54 mm (n=5). Vertebral development was complete at sixty days post-hatching, with an average total length of 20.25±0.45 mm (n=5). This study sheds light on the skeletal development of H. villosus, providing valuable standards and fundamental data for understanding skeletal deformities in this species.

사할린가자미(Limanda sakhalinensis)의 난발생 과정 및 자치어 형태발달

한경호 ( Kyeong-ho Han ),나해춘 ( Hae-choon Na ),박애전 ( Ae-jeon Park ),박재민 ( Jae-min Park ) 한국수산과학회 2017 한국수산과학회지 Vol.50 No.3

Egg development and morphological change of larvae of the Sakhalin sole Limanda sakhalinensis were studied by observing specimens obtained in a rearing experiment from fertilized eggs to the juvenile stage. The wild broodstock was collected in January 2010 and kept in a circular water tank (Ø1.5×1 m) at a temperature of 14.5±0.5℃. Fertilized eggs ranged from 0.72 to 0.82 mm (0.77±0.07 mm, mean±SD) in diameter. The eggs were spherical, transparent and adhesive demersal. The egg yolk was divided from the oocyte 10 min after fertilization (AF), and an embryo was formed in 36 h AF. More than 50% of the eggs hatched within 133 h AF. The mouth and anus did not open until 3.5±0.25 mm total length (TL). At 4, days after hatching (AH), the fish became larvae 3.7 to 4.2 mm (4.0±0.36 mm TL), yolk absorption was completed and the mouth began to open. The left eye moved upward and the nostril moved to the right at 39 days AH. These post-larvae ranged from 8.0 to 9.9 mm TL (8.9±1.33 mm TL). At 50 days AH, the fish became juveniles (12.4±1.20 mm TL) There were 70-72 dorsal fin rays, 55-56 anal fin rays, 11 pectoral fin rays, and 6 ventral fin rays and the juveniles adopted a benthic life.

제주광어 품종개량을 위한 유전체선발 육종 연구

이숙경,정태혁,임채현,D.S. Liyanage,W.K.M. Omeka,H.M.V. Udayantha,이지훈,김정은,김가은,Y.K. Kodagoda,H.A.C.R. Hanchapola,현재민,박애전,공포,장영환,C. Massault,David B. Jones,Kyall R. Zenger,Dean R. Jerry,이제희 한국수산과학회 양식분과 2022 한국수산과학회 양식분과 학술대회 Vol.2022 No.5