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Backgroud/Aims: Mucin genes are expressed in a variety of epithelial organs in a highly tissue-specific fashion. Previously in situ technique of mucin genes reveal the specific cellular source of the RNA species compared to immunohistochemical and bloting technique in normal colon and colon cancer. Therefore this study was undertaken to know the cellular localization of intestinal mucin gene(MUC2) in the premalignant lesion of colon cancer, colon polyp and transitional epithelium and to evaluate the possible role of MUC2 as genetic marker in terms of colon adenoma-cancer sequence. Methods: Immunohistochemical studies using antibody against synthetic MUC2(antiMRP) and in situ hybridization using the [^(35)S] probes containing the tandem repeat region of MUC2 has been used in 10 normal colonic tissues, 42 transitional mucosa and 21 hyperplastic polyps and 26 adenomatous polyps and 56 colon cancers. Results: In normal colon, MUC2 mRNA and antiMRP recognizing the MUC2 apomucin stained only goblet cells. In hyperplastic polyp MUC2 and apomucin are expressed as much as normal colonic tissue. But in adenomatous polyp the expression of mucin genes showed decreased with severity of dysplasia. Transitional mucosa near to cancer tissue showed no difference of expression of MUC2 gene and MUC2 apomucin compared to those of normal tissues. However, in colon cancer MUC2 epito frequently expressed and were restricted to the cytoplasm of malignant glands but in situ hybridization of MVC2 mRNA were expressed in a few localized areas of tumor glands at decreased levels in comparision to normal tissues. Conclusians; These results showed that MUC2 gene may be a possible genetic marker, indicating the grade of dysplasia and differentiation of cancer and altered regulation of their expression in cancer cells.