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백성진(Seong Jin Baek),김용석(Yong Seok Kim),용환미(Hwan Mee Yong),채주병(Ju Byung Chae),이선애(Sun Ae Lee),배우철(Woo Chul Bae),박동우(Dong Woo Park),김동연(Dong Yeon Kim),이준우(June Woo Lee),박승국(Seung Kook Park) 대한약학회 2002 약학회지 Vol.46 No.1
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β-Immunan, a proteoglycan, was isolated from the mycelium of Ganoderma lucidum which belongs to a medicinal mushroom. The effects of β-Immunan on cell- cell and cell-matrix interactions mediated by carbohydrate-recognition and the mechanism responsible for the inhibition of experimental metastasis of Bl6-FIO and B16/BL6 murine melanoma were studied. The results showed that Ganoderma lucidum-Immunan inhibited Bl6-Fl melanoma cell's adhesion to laminin and asialofetuin-induced homotypic aggregation and reduced invasion against Bl6-FIO murine melanoma cells through matrigel in vitro assay: When β-Immunan was intraperitoneally administrated to C57BL/6 mice bearing B16/BL6 murine melanoma cells, it was decreased the number of pulmonary metastatic colony by the dose dependent manner ranging from 20 to 100 mg/kg/day, The results indirectly indicate that clinical treatment with β-Immunan might be expected to exhibit anti-metastatic effect. In the put-monary metastasis, the number of pulmonary metastatic colony of melanoma when β-Immunan was intraperitoneally aduunistrated to C57BL/6 mice bearing Bla/BL6 murine melanoma cells by intravenous injection were decreased by the dose dependent manner ranging from 20 to 100 mg/kg/day.
백성진(Seong Jin Baek),김용석(Yong Seok Kim),용환미(Hwan Mee Yong),채주병(Ju Byung Chae),윤환민(Hwan Min Yun),박동우(Dong Woo Park),김동연(Dong Yeon Kim),이정옥(Jeong Ok Lee),이준우(June Woo Lee),박승국(Seung Kook Park) 대한약학회 2001 약학회지 Vol.45 No.6
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In this study, the antitumor activities were investigated using β-Immunan, a proteoglycan obtained from cultured mycelia of the IYO09 strain of Ganoderma lucidum IY009belonging to basidiomycetes. The result showed the significant effect of cytotoxicity test against murine sarcoma 180 and murine Iymphocytic leukemia L1210 using immunized macrophage cultures by β-Immunan. When intraperitoneally injected at 40 mg/kg/day daily for 10 days, β-Immunan inhibited the growth of sarcoma 180 solid tumor in ICR mice by 88.8% (p<0.05). It was also observed that β-Immunan increased life span by 85.2% (p<0.01) after treatment of 100 mg/kg/day in BDFI mice bearing Iymphocytic leukemia L1210. And combination therapy with cisplatin (dosage: 4 mg/kg) increased life span by 140.4% (p<0.05) after treatment of 100 kg/mg/day in BDFI mice bearing Iymphocytic leukemia L1210.
N/A Abnormalities of cholesterol metabolism has been often suspected in patients with liver diseases. Hence we determined the free and esterified cholesterol in patients with liver diseases including acute and chronic hepatitis, hepatic cirrhosis, hepatoma, and obstructive jaundice comparing with healthy subjects. The method in this study has tried to extract the free and esterified cholesterol by organic solvent, n-Octane, the total cholesterol was extracted by hexane after hydrolysis of the esterified cholesterol by ethanolic KOH. Free, esterified and total cholesterol were measured by high pressure liquid chromatography. Patients with liver diseases have higher level of unesterified cholesterol than healthy subjects. Cholesteryl linoleate per cholesteryl oleate ratio in hepatic cirrhosis and acute hepatitis patients were significantly lower than those of healthy subjects. Levels of cholesteryl arachidonate in hepatic cirrhosis and obstructive jaundice patients were significantly lower than healthy subjects.
N/A The peptic diseases in infants and children are no longer rare conditions in these days. Upper G-I barium study is usual method of choice to make diagnosis of peptic disorders in children in Korea. We report the endoscopic experiences on pediatric age group, 90 cases, in the endoscopic unit during five years period until september l984 1) Olympus GIF type Q, type P-10 and Fujinon UGI type FP were available endoscopes. 2) 0.3% procaine viscous was used for pharyngeal anesthesia over 5 years of age without diazepam injection or general anesthesia. No premedication was used for under 5 years of age. 3) Chief complaints or signs of patients were abdominal pain, hematemesis and/or melena and vomiting in order. 4) Of ninty patients, 40 children were found to be normal endoscopic finding, 15 were gastritis, 15 duodenal ulcer, 6 gastric ulcer, 4 esophageal varices, 3 duodenitis and 2 achalasia respectively. 5) Among 22 upper G- I bleeders, 37% were due to peptic ulcer origin. Four of 5 unknown original bleeders were identified to be nasal origin. 6) There were no significant complications or sequelae during and after endoscopic procedures. 7) Pediatric endoscopy by experienced personels was thought to be safe, effective and easy matter.
N/A Percutaneous needle biopsy of the liver is known to be a commonly utilized and safety procedure that can be performed at the patients bedside. But the compression of the biopsy site makes the patient uncomfortable, on the other hand intrahepatic or subcapsular hematoma formation could be serious and a life threatening complication. Recently, real-time ultrasound technigues have been used for early detection of such complication. We compared blood pressure, pulse rate and hematoma incidence between the compression group (45 patients) and the noncompression group (49 patients) after percutaneous liver biopsy. The results were as follows: 1) Intrahepatic hematoma developed in one case in each group and their diameter were less than 10 mm. 2) There was no significance in hematoma incidence between the 2 groups. 3) There was no significant difference in blood pressure and pulse rate between the two groups. In conclusion, we think that noncompression after percutaneous liver biopsy is a safe method.
Eosinophilic gastroenteritis is an uncommon disease charaterized by abdominal pain, vomiting, diarrhea, eosinophilia in peripheral blood and eosinophilic infiltration of gut. We experienced a case of eosinophilic gastroenteritis, which developed after ingestion of medicinal plant, Acanthopanax sessili florum, reported with review of literature.