국립공원 아고산대 독립훼손지 복원식생 분포특성 및 관리방안

김종엽(Jong-Yup Kim),윤주웅(Ju-Ung Yun),한봉호(Bong-Ho Han),곽정인(Jeong-In Kwak),박석철(Seok-Cheol Park),박수영(Su Young Park),정원옥(Won-Ok Jeong) 국립공원연구원 2022 국립공원연구지 Vol.13 No.1

본 연구는 독립훼손지 중 아고산대에 위치한 지리산국립공원 세석평전과 노고단 복원식생지를 대상으로 현존식생 분석 및 식생분포 특성을 파악하여 중장기적인 모니터링 및 관리방안 수립을 위한 기초자료를 제공하고자 수행하였다. 현존식생 조사범위는 훼손지 일대 유역권분포 현황을 고려하여 설정하였으며, 세석평전 조사면적은 55,037㎡, 노고단 조사면적은 166,599㎡ 이었다. 세석평전은 초본층에 의한 식생피복도는 90% 이상 수준으로서 나지노출이 없었고, 관목층의 피복도가 60% 이상으로 안정화되어 있었다. 주요 출현수종은 구상나무, 사스래나무, 쇠물푸레나무, 철쭉, 털진달래, 붉은병꽃나무 등 식재수종과 자연적으로 이입한 자생수종이 생육하고 있었다. 노고단은 신갈나무, 호랑버들, 철쭉, 털진달래, 미역줄나무 등이 우점하는 관목식생지가 52%로 가장 넓게 분포하고 있었고, 전반적으로 식생활착 상태가 양호하였다. 노고단의 원식생은 신갈나무군락으로 추정되었고, 군부대철거지에는 귀화식물이 분포하고 있기 때문에 적극적인 관리가 필요하였다. 연구대상지는 장기적으로 주변 자연식생과 조화로운 식생경관으로 발달할 것으로 예측되었다. 향후 훼손지 복원모델은 인근 지역의 원식생을 고려하여 설정해야 할 것이지만, 복원 달성 목표는 실현가능한 수준의 목표를 설정해야 할 것이다. This study was conducted to provide basic data for mid-to long-term monitoring and establishment of management plans by analyzing the existing vegetation and identifying the vegetation distribution characteristics for the restored vegetation in Nogodan and Seseokpyeongjeon in Jirisan National Park located in the subalpine zone among the independent damaged areas. Actual vegetation survey range was established in consideration of the distribution of the watershed around the damaged area. The surveyed area of Seseokpyeongjeon was 55,037㎡, and the surveyed area of Nogodan was 166,599㎡. In Seseokpyeongjeon, the vegetation coverage by the herbaceous layer was almost 90% or more, there was no bare exposure, and the coverage of the shrub layer was stabilized at 60% or more. The main emerging tree species were planted species such as Abies koreana, Betula ermanii, Fraxinus sieboldiana, Rhododendron schlippenbachii, Rhododendron mucronulatum var. ciliatum, and Weigela florida as well as natively migrated native species. In Nogodan, the shrub vegetation dominated by Quercus mongolica, Salix caprea, Rhododendron schlippenbachii, Rhododendron mucronulatum var. ciliatum, and Tripterygium regelii was the most widely distributed with 52% of the area, and the overall diet was good. The native vegetation of Nogodan was presumed to be a cypress colony. Naturalized plants were distributed in the military base demolition site, so active management was required. The study site was predicted to develop into a vegetation landscape in harmony with the surrounding natural vegetation in the long term. In the future, the restoration model for damaged areas should be set in consideration of the native vegetation of the nearby area, but the goal to achieve restoration should be set at a feasible level.

표준화 기반 유의한 유전자 선택 방법 조합을 이용한 마이크로어레이 분류 시스템 설계

박수영,정채영,Park, Su-Young,Jung, Chai-Yeoung 한국정보통신학회 2008 한국정보통신학회논문지 Vol.12 No.12

정보력 있는 유전자는 특정한 실험 조건의 특성을 나타내주는 발현수준의 유전자를 의미한다. 이 유전자들은 여러 집단 간의 발현수준에서 유의한 차이를 보여주며, 실제로 집단 간의 차이를 유발하는 유전자일 확률이 높아 특정 생물학적 현상과 관련 있는 정보적 유전자를 찾는 연구에 이용될 수 있다. 본 논문에서는 먼저 그 동안 제안된 여러 표준화 방법들 중에서 가장 널리 사용되고 있는 방법들을 이용하여 데이터를 표준화 한 후 제안한 유사성 척도 조합 방법으로 정보력 있는 유전자들을 추출할 수 있는 시스템을 고안하였다. 다층퍼셉트론 신경망 분류기를 이용하여 각 표준화 방법들의 성능을 비교분석하였다. 그 결과 Lowess 표준화 후 피어슨 적률 상관 계수와 유클리디안 거리 계수 조합을 이용하여 선택된 200 유전자들을 멀티퍼셉트론 신경망 분류기로 분류한 결과 93.84%의 향상된 분류 성능을 보였다. Significant genes are defined as genes in which the expression level characterizes a specific experimental condition. Such genes in which the expression levels differ significantly between different groups are highly informative relevant to the studied phenomenon. In this paper, first the system can detect informative genes by similarity scale combination method being proposed in this paper after normalizing data with methods that are the most widely used among several normalization methods proposed the while. And it compare and analyze a performance of each of normalization methods with multi-perceptron neural network layer. The Result classifying in Multi-Perceptron neural network classifier for selected 200 genes using combination of PC(Pearson correlation coefficient) and ED(Euclidean distance coefficient) after Lowess normalization represented the improved classification performance of 98.84%.

신경망 기반의 유전자조합을 이용한 마이크로어레이 데이터 분류 시스템

박수영,정채영,Park, Su-Young,Jung, Chai-Yeoung 한국정보통신학회 2008 한국정보통신학회논문지 Vol.12 No.7

최근 생명 정보학 기술의 발달로 마이크로 단위의 실험조작이 가능해짐에 따라 하나의 chip상에서 전체 genome의 expression pattern을 관찰할 수 있게 되었고, 동시에 수 만개의 유전자들 간치 상호작용도 연구 가능하게 되었다. 본 논문에서는 암에 걸린 흰쥐 외피 기간 세포 분화 실험에서 얻어진 3840 유전자의 마이크로어레이 cDNA를 이용해 데이터의 정규화를 거쳐 본 논문에서 제안한 유사성 척도 조합 방법으로 정보력 있는 유전자들을 추출한 후, 유사성 척도 조합 방법과 결합한 멀티퍼셉트론 신경망 분류기와 기존의 DT, NB, SVM 분류기를 이용하여 클래스 분류 시스템을 구축하고, 성능을 비교분석하였다. 피어슨 적률 상관 계수와 유클리디안 거리 계수 조합을 이용하여 선택된 200 유전사들을 멀티퍼셉트론 신경망 분류기로 분류한 결과 98.84%의 정확도를 보여 다른 분류기를 이용하여 실험을 수행한 경우보다 향상된 분류 성능을 보였다. As development in technology of bioinformatics recently mates it possible to operate micro-level experiments, we can observe the expression pattern of total genome through on chip and analyze the interactions of thousands of genes at the same time. In this thesis, we used CDNA microarrays of 3840 genes obtained from neuronal differentiation experiment of cortical stem cells on white mouse with cancer. It analyzed and compared performance of each of the experiment result using existing DT, NB, SVM and multi-perceptron neural network classifier combined the similar scale combination method after constructing class classification model by extracting significant gene list with a similar scale combination method proposed in this paper through normalization. Result classifying in Multi-Perceptron neural network classifier for selected 200 genes using combination of PC(Pearson correlation coefficient) and ED(Euclidean distance coefficient) represented the accuracy of 98.84%, which show that it improve classification performance than case to experiment using other classifier.

반려동물의 종류 및 특징

박수영,Park, Su-Yeong 한국단미사료협회 2003 사료산업 Vol.4 No.-

Kluyverromyces fragilis의 Alkaline Phosphatase 유전자의 구조 분석

박수영,황선갑,하상철,김종국,박완,홍순덕,Park, Soo-Young,Hwang, Seon-Gap,Ha, Sang-Chul,Kim, Jong-Guk,Park, Wan,Hong, Soon-Duck 한국미생물·생명공학회 1994 한국미생물·생명공학회지 Vol.22 No.1

From the pSKH201 plasmid which had been previously cloned in our laboratory, a 3.0kbp insert DNA encoding the alkaline phosphatase of Kluyveromyces fragilis was cleaved with several restriction endonucleases and ligated int the appropriate sites of M13mp18/19 vectors and sequenced by Sanger's dideoxy chain termination method. The sequence contained a 1,638 bp open reading frame(ORP) whose similarities in nucleotide, when compared with those of Saccharomyces cerevisiae and Escherichia coli by GENETYX program, were found to be 61% and 46%, respectively. The deduced amino acid sequence consists of 546 amino acids and contains several homologous regions in the alkaline phosphatases of E. coli, S.cerevisiae and human placenta.

PKI환경에서 ElGamal 방식의 ECC를 이용한 안전한 메신저 설계에 관한 연구

박수영,최광미,정채영,Park Su-Young,Choi Kwang-Mi,Jung Choi-Yeoung 한국정보통신학회 2006 한국정보통신학회논문지 Vol.10 No.8

컴퓨터와 네트워크의 보급이 일반화되면서 인터넷을 통한 정보 전달이 일상생활처럼 되고 있다. 기존에는 정보를 전달하기 위한 방법이 주로 전자메일에 한정되어 있던 것에 반해, 요즘은 좀 더 즉각적으로 메시지를 전달해주는 메신저를 많이 사용하고 있다. 현재 사용되고 있는 대부분의 메신저는 자료의 효과적인 관리를 위한 통신의 주체가 되는 각각의 에이젼트들간의 통신과 서버와 에이젼트의 통신에 있어 악의적인 침입에 의한 정보누출이 문제가 되고 있다. 본 논문에서는 메신저의 안전한 통신을 위해 PKI를 이용한 ElGamal 방식의 ECC 이용하여 최대한 안전성이 확보될 수 있고 사용자그룹 단위의 암호화를 위해 그룹별로 타원곡선과그 위에 있는 임의의 점을 선택하여 다른 그룹과 구별할 수 있는 안전한 메신저 시스템을 설계하였다. As computers and networks become popular, distributing information on the Internet is common in our daily life. In the past, e-mail has been the primary choice of exchanging information but messengers are gaining popularity abroad and domestically because of their nature of getting immediate responses. Information leakage by invasion that is enemy of evil in communication of communications division Server and Agent between each agents that become burden of communication for effective administration of data for most of existing messenger is becoming an issue. In this paper, we design a secure messenger system that could be obtained maximum security. It use ECC based on ElGamal methodology using PKI for secure communication. For the message encryption and decryption between the same group non, each group is kept distinct by drawing an elliptic curve and an arbitrary point is chosen on the curve.

Espression of Alkaline Phosphatase Gene from Kluyveromyces fragilis in E. coli and S. cerevisiae

박수영,황선갑,이동선,김종국,남주현,홍순덕,Park, Soo-Young,Hwang, Seon-Kap,Lee, Dong-Sun,Kim, Jong-Guk,Nam, Joo-Hyun,Hong, Soon-Duck 한국미생물 · 생명공학회 1995 한국미생물·생명공학회지 Vol.23 No.2

The alkaline phosphatase (K-ALPase) gene of Kluyveromyces fragilis has been cloned (1) and determined its base sequences (2) previously in our laboratory. When the K-ALPase gene was expressed in Escherichia coli and Saccharomyces cerevisiae, it showed a constitutive activity in E. coli, and a derepressed activity in S. cerevisiae in phosphate-limited medium. Northem hybridization experiment was performed to elucidate the transcription level of the K-ALPase gene. Northern experiment showed that transcription level of K-ALPase gene in S. cerevisiae was higher in phosphate depletion, but it was higher in high phosphate medium than in phosphate limited medium in K. fragilis. The transcription initiation site of the K-ALPase gene was determined by primer extension analysis. It matched nucleotide position - 169 in relation to the putative trnslational start site.

반려동물의 종류 및 특징

박수영,Park, Su-Yeong 한국단미사료협회 2004 사료산업 Vol.2 No.4

고구마 배양세포에서 Peroxiredoxin cDNA의 분리 및 발현 특성

박수영,류선화,권석윤,김종국,곽상수,Park, Soo-Young,Ryu, Sun-Hwa,Kwon, Suk-Yoon,Kim, Jong-Guk,Kwak, Sang-Soo 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2

Peroxiredoxin(Pix) are large family of peroxidases that reduce alkyl hydroperoxides and hydrogen peroxide. A cDNA clone (referred to as swPrxl) encoding Pix was from a sweetpotato cDNA library constructed from suspension-sultured cells, and its expression was investigated in terms of stress. The swPrxl contained an open reading frame (ORF) encoding mature protein of 193 amino acids with calculated molecular mass of 20.8kDa. The predicted amino acid sequence of swPrxl has two conserved cysteines that are essential resicues for the reduction of peroxides. It showed high amino acid sequence homology ot PixIIF of Arabidopsis (77%) and putative Prx of rice(72%). RNA gel-blot analysis showed that swPrxl gene was expressed dominantly in leave among intact tissues, and also highly detect in suspension-cultured cells. Interestingly, the level of swPrxl transcripts was almost the same regardless of the growth stage in suspension culture. Furthermore, the transcription level of swPrxl gene was not significantly changed in response to various stress treatments such as wounding, extreme temperature and stress-related chemicals RT-PCR analyses.

Propenone 유도체의 $NF-{\kappa}B$ 활성 억제 및 IL-8 유도에 의한 단핵구의 장 상피세포 부착 억제 효과

박수영,김경진,이종숙,이응석,김정애,Park, Su-Young,Kim, Kyoung-Jin,Lee, Jong-Suk,Lee, Eung-Seok,Kim, Jung-Ae 대한약학회 2008 약학회지 Vol.52 No.1

In this study, we examined the inhibitory effects of propenone derivatives, 1,3-diphenyl-propenone (DPhP), 3-phenyl-1-thiophen-2-yl-propenone (PhT2P), 3-phenyl-1-thiophen-3-yl-propenone (PhT3P) and 1-furan-2-yl-3-phenyl-propenone (FPhP), on $TNF-{\alpha}$-induced nuclear factor (NF)-${\kappa}B$ activity and interleukin (IL)-8-induced monocyte adhesion to colon epithelial cells. 1-Furan-2-yl-3-pyridin-2-yl-propenone (FPP-3) that is previously reported as a $NF-{\kappa}B$ inhibitor suppressed $TNF-{\alpha}$-induced monocyte-epithelial cell adhesion in a concentration-dependent manner. The propenone derivatives, DPhP, PhT2P, PhT3P, FPhP, also inhibited $TNF-{\alpha}$-induced $NF-{\kappa}B$ activation in a similar degree to FPP-3. In a DPPH radical scavenging assay, none of the compounds showed DPPH radical scavenging activity, indicating that the inhibitory actions of the propenone derivatives on redox-sensitive $NF-{\kappa}B$ activity is not due to a simple free radical scavenging activity. In addition, the propenone derivatives also suppressed the IL-8-induced monocyte adhesion to colon epithelial cells. Furthermore, the effective concentrations of the propenone derivatives on both $NF-{\kappa}B$ activation as well as IL-8 induced monocyte-epithelial cell adhesion were 1000 times lower than 5-aminosalicylic acid (5-ASA), a clinically used drug for inflammatory bowel disease. These results suggest that the propenone derivatives may be a potential lead having a strong inhibitory activity against inflammatory cytokine-induced epithelial inflammation.