육식성과 초식성 경골여류 소장의 미세구조 및 alkaline phosphatase 분포에 관한 연구

박노관,류동석 청주대학교 산업과학연구소 2005 産業科學硏究 Vol.22 No.3

The ultrastructure and alkaline phosphatase(ALP) distribution in anterior part of small intestine of angelfish (Pterophyllum eimekei) and Metynnis schreitmulleri were investigated by light and transmission electron microscopes. The anterior part of small intestine in angelfish and Metynnis schreitmulleri were divided into mucosa, submucosa, muscularis externa and serosa, and the mucosa was two layers with epithelium and lamina propria. The epithelium consisted of simple columnar epithelial cells with brush border developed well, and Goblet cells of different size were scattered between absorptive epithelial cells. The muscularis mucosa was not observed. In angelfish, the villi were developed well, and two types of Goblet cells in epithelium and lipid droplet in cytoplasm of epithelial cells were found. In Metynnis schreitmulleri, the villi were not developed well, one type of Goblet cell in epithelium was found, and the lipid droplet in cytoplasm of epithelial cells was not found. The distribution of ALP in anterior part of small intestine of angelfish displayed intense activity all over brush border of mucosa epithelium and apical cytoplasm, but the activity of ALP in anterior part of small intestine of Metynnis schreitmulleri was weak in only brush border.

참붕어(Pseudorasbora parva) 심장의 조직학적 연구

박노관 ( No Kwan Park ),류동석 ( Dong Suck Reu ) 한국어류학회 2015 韓國魚類學會誌 Vol.27 No.1

참붕어 심장의 조직학적 구조를 광학현미경을 이용하여 관찰하였다. 참붕어의 심장은 정맥동, 심방, 심실 및 동맥구가 연속적으로 배열되어 있었다. 정맥동은 조직학적으로 심내막, 심근층, 심외막층으로 구분되었고, 심방과 심실은 심내막, 심내막하층, 심외막하층 및 심외막으로 구분되었으며, 동맥구는 심내막, 심내막하층, 중간층, 심외막하층 및 심외막으로 구분되었다. 정맥동과 심방, 심방과 심실 및 심실과 동맥구 사이에는 판막이 존재하였다. 정맥동은 대부분 아교질로 구성된 외벽을 가지고 있었으며 정맥동과 심방의 연결부위에는 고리 모양의 조직이 분포하였다. 심방은 얇은 심근층에 의해서 형성된 비연속적인 테두리가 소주들에 의해 형성된 해면성 심근을 둘러싸고 있었고, 심외막하층과 소주에는 아교질이 분포하였다. 심실의 심근층은 해면성 심근으로 이루어져 있고, 심외막하층에는 혈관이 분포하였으며, 심실의 심외막하층과 소주에는 아교질이 분포하였다. 동맥구에서 심내막하층에 의해 형성된 이랑은 직경과 길이가 매우 다양했고, 심내막 세포는 볼록한 모양이었으며, 심내막하층의 세포들은 불규칙한 모양이었으나 무리를 지어 분포하지는 않았다. 중간층의 세포들은 배열방향이 다르게 불규칙한 층을 이루었고, 심외막하층에서는 서로 다른 모양의 세포들이 관찰되었다. 동맥구의 심내막하층, 중간층 및 심외막하층에는 아교질과 탄력소가 풍부하였고, 심외막은 한 층의 편평한 세포들로 이루어져 있었다. The histological structure of the heart in Pseudorasbora parva was investigated by light microscope. The heart consisted of four consecutive chambers, the sinus venosus, atrium, ventricle and bulbus arteriosus. The wall of the sinus venosus was divided into endocardium, myocardium and epicardium, and the walls of the atrium and ventricle were divided into endocardium, subendocardium, myocardium, subepicardium and epicardium, and the wall of the bulbus arteriosus was dividedinto endocardium, subendocardium (ridge tissue), middle layer, subepicardium and epicardium. The valves were observed in the sinoatrial, artrioventricular and bulboventricular junctions. The sinusvenosus wall was mostly made up of collagen. The rings of tissue were observed at the sinoatrialjunction. The atrium was composed of a spongy trabeculate myocardium surrounded by an externalrim of thin myocardium, and collagens were distributed in the subepicardium and trabeculae. The ventricle was a spongy myocardium with vessels in subepicardium. In the subepicardium and trabeculae of the ventricle, collagens were distributed. In the bulbus arteriosus, the diameter and length of the ridges were differed. The endocardial cells were convex and the non-clustered subendocardial cells showed irregular shapes. The cells of the middle layer were arranged into incomplete layers that showed different orientations. The subepicardium was formed by cells of different morphology. Collagens and elastins were demonstrated in the subendocardium, middle layer and subepicardium of the bulbus arteriosus. The epicardium was a single layer composed of flattened cells.

바다빙어과 빙어 (Hypomesus nipponensis)의 심실 구조

박노관 ( No Kwan Park ),류동석 ( Dong Suck Reu ) 한국어류학회 2015 韓國魚類學會誌 Vol.27 No.3

빙어의 심실 구조를 광학현미경과 주사전자현미경으로 관찰하였다. 빙어의 심장은 정맥동, 심방, 심실 및 동맥구가 연 속적으로 배열되어 있었다. 심실의 외벽은 심내막, 심근층, 심외막하층 및 심외막으로 구분되었고, 심방과 심실 사이의 방실판막 및 심실과 동맥구 사이의 구실판막이 관찰되었다. 심실은 주머니 모양이었으며, 심근층은 해면성 심근으로만 구성되어 있었고 관상혈관은 분포하지 않았다. 심실에서 소주들은 중심 내강과 작은 내강들을 형성하였으며, 소주는 원기둥 모양이었다. 심외막하층, 방실판막 및 구실판막에서 아교질의 분포가 관찰되었으나 소주에서는 심실의 기저부에서 부분적으로 관찰되었다. 특히 소주들 사이에서는 심내막교가 관찰되었다. 이와 같은 결과는 육봉형 빙어가 서식환경 및 생활양식에 적응한 결과로 생각된다. The structure of the ventricle in the heart of Hypomesus nipponensis was investigated by light and scanning electron microscope. The heart consisted of four consecutive chambers, the sinus venosus, atrium, ventricle and bulbus arteriosus. The wall of the ventricle was divided into endocardium, myocardium, subepicardium and epicardium. The valves were observed in the artrioventricular and bulboventricular junctions. The ventricular myocardium was an entirely spongy without coronary vessels. The trabecular network was formed with lumina included a central lumen and the trabecula was cylindrical shape. Collagen distribution was apparent in the subepicardium, artrioventricular valve and bulboventricular valve. But in the trabeculae, collagen distribution was observed partly in the base of the ventricle. Especially, the endocardial bridges were observed between trabeculae. These results might be considered that the structure of the ventricle in the heart of pond smelt is adapted to sedentary habit associated with its habitat and lifestyle.

빙어(Hypomesus nipponensis)의 정소 내 정자의 미세구조

김재구,박노관,류동석,Kim, Jae-Goo,Park, No-Kwan,Reu, Dong-Suck 한국현미경학회 2010 Applied microscopy Vol.40 No.4

The ultrastructure of sperms in testes of the pond smelt (Hypomesus nipponensis) was investigated using electron microscopes. The whitish testis was located between swim bladder and intestine. Especially, the left testis was larger than the right testis. The sperm was approximately $26\;{\mu}m$ in length. The sperm had an oval head and the acrosome was not found. The nucleus was about 400 nm in diameter and chromatin was incompletely condensed. The nuclear fossa deeply formed in sperm head and two centrioles were located in the fossa. The mitochondrium was observed only one in midpiece of the sperm and a motile flagellum consisted of an axoneme with a typical 9+2 pattern of microtubule. Also, the tail of the sperm has axonemal fins. 바다잉어과(Osmeridae)에 속하는 빙어(Hypomesus niponesis)의 정소 내 성숙한 정자들을 전자현미경을 이용하여 조사하였다. 빙어의 정소는 한 쌍으로 부레와 창자 사이에 위치하였고 좌측 정소가 우측 정소보다 큰 것으로 나타났으며 흰색이었다. 정소 내의 성숙한 정자의 길이는 $26{\mu}m$ 정도였다. 두부는 난형으로 직경은 400 nm 정도였으며 첨체는 관찰되지 않았고, 특히 염색질의 이질적 응축이 관찰되었다. 두부의 핵이 함입되어 핵와(nuclear fossa)가 깊게 형성되었으며 핵와 내 기저부에서 편모가 직선으로 분포하였고 편모의 미세소관은 전형적인 9+2 구조를 이루고 있었다. 또한 편모의 외측에는 두개의 axonemal fin이 관찰되었으며, 중편에는 미토콘드리아가 하나만 존재하였다.

Fenvalerate가 피라미(Zacco platypus) 뇌 조직에서 apoptosis 정도와 GFAP 발현에 미치는 영향

김성우,박노관,이상래,류동석,Kim, Sung-Woo,Park, No-Kwan,Lee, Sang-Rae,Reu, Dong-Suck 한국현미경학회 2012 Applied microscopy Vol.42 No.2

Fenvalerate에 대한 피라미(Zacco platypus)의 독성 수준을 반수 치사량 (LC50)을 이용하여 평가하고, 뇌 손상의 기작과 GFAP의 발현 정도를 광학현미경과 형광현미경을 이용하여 조사하였다. 피라미의 생존율은 fenvalerate의 농도가 높아질수록 낮아졌고 LC50은 $27.79{\mu}g/L$이었다. TUNEL assay결과 fenvalerate의 농도가 높아질수록 뇌 조직에서 세포고사가 증가하였으며, periventricular zone에서 GFAP 발현이 증가하였다. 따라서 세포고사는 fenvalerate에 의한 피라미 뇌 손상의 주요 기작 중 하나로 생각되며, periventricular zone에서 GFAP의 발현증가는 뇌 손상의 지표로 이용될 수 있을 것이다. 이는 pyrethroid계 살충제에 의한 어류의 뇌 손상기작 연구와 수생 생태계 보존을 위한 기초자료로 활용될 수 있을 것으로 사료된다. We evaluated the level of toxicity by LC50 and investigated the mechanism of brain impairment and GFAP expression by light and fluorescence microscopes in the pale chub, Zacco platypus, treated with fenvalerate. Survival rate was decreased according to the rise of fenvalerate concentration, and LC50 concentration was $27.79{\mu}g/L$. Apoptosis was increased according to the rise of fenvalerate concentration by TUNEL assay which determine apoptotic cell death population. Also, GFAP expression was increased in the periventricular zone. These results suggest that apoptosis might be a major mechanism to brain impairment of the pale chub by fenvalrerate. Increased GFAP expression in the periventricular zone would be an index of brain impairment. Taken together, this study might contribute to reveal the pathological mechanism of fish brain impairment by insecticide of pyrethroid, and to be an useful basic data for preservation of aquatic ecosystem.

주령에 따른 쥐 경동맥 손상 모델에서의 신생내막 형성율의 차이

권진숙,박노관,정일하,김유경,배장환,황경국,김동운,조명찬 대한심장학회 2007 Korean Circulation Journal Vol.37 No.3

Background and Objectives:The degree of neointima formation after infliction of a carotid artery balloon injuryin rats varies greatly depending on the sex, age, species and operational method. Strong variation is common, evenwithin only a single control. This study attempted to find if there was any significant difference in neointimaformation following a carotid artery balloon injury in 6 to 12 week old rats; the age commonly used in these typesof experiments. Materials and Methods:A balloon injury was inflicted on the carotid arteries of male Sprague-Dawley rats at 6 (n=9, 250-270 g), 8 (n=8, 280-300 g) and 11 weeks (n=10, 320-340 g) of age. Two weeks postoperation,a histomorphometric analysis was carried out. The vascular smooth muscle cell proliferation was measuredin situ via BrdU incorporation 2 days after injury infliction. Results:The neointima areas of the 6 week(0.22±0.04 mm2) and 8 week old groups (0.17±0.08 mm2) were 3.1 and 2.4 times larger than that of the 11 weekold group (0.07±0.03 mm2). The mitotic index was significantly reduced in 11 week old group (n=4, 9.22±1.51%)compared to those of the 6 (n=4, 25.03±3.92%) and 8 week old (n=4, 21.66±3.66%) groups. Conclusion:Special care should be taken when interpreting neointima formation, as even a slight variation in the age and weightin 6 to 12 week old (250-340 g) rats; the age commonly used in these types of experiments, results in an unexpectedlylarge difference. (Korean Circulation J 2007;37:78-83) 배경 및 목적: 쥐 경동맥 풍선 손상 후 신생내막의 형성 정도는 쥐의 성별, 연령, 종(species) 및 시술 방법 등에 따라 큰 차이를 보이고 있다. 심지어 동일 실험의 대조군(control) 내에서도 심한 편차를 보이는 경우가 많다. 연령에 관한 연구는 주로 노화에 관한 연구로 젊은 쥐와 아주 늙은 쥐를 비교하였다. 본 연구는 실험에 많이 사용되는 주령인 6~12 주령의 쥐를 대상으로 풍선 손상 후 신생 내막의 형성에 있어 유의한 차이가 있는지를 알아보고자 하였다. 방 법: 6주령(n=9), 8주령(n=8), 11주령(n=10)의 수컷 Sprague- Dawley rat의 경동맥을 통상적인 방법에 의해 풍선 손상을 가하였다. 시술 2주째 관류고정 후 경동맥을 적출하여 형태 조직학적 분석을 시행하였다. 혈관 손상 2일 후 BrdU 면역화 학염색을 이용하여 생체내 혈관평활근세포 증식을 측정하였다. 결 과: 형태조직학적 분석 결과 6주령군(n=9)의 신생내막의 면 적(neointima area)은 0.22±0.04 mm2, 8주령군(n=8)은 0.17±0.08 mm2, 11주령군(n=10)은 0.07±0.03 mm2으로 6 주령군과 8주령군은 11주령에 비해 신생내막의 형성이 각각 3.1배와 2.4배나 증가하였고(p<0.05), 6주령과 8주령 양군 간에는 통계적으로 유의한 차이는 없었다. BrdU를 이용하여 측정한 mitotic index도 6주령군(n=4) 25.03±3.92%, 8주 령군(n=4) 21.66±3.66%, 11주령군(n=4) 9.22±1.51%으로 11주령군이 6주령과 8주령에 비해 혈관평활근세포의 증식이 유의하게 감소되어 있었고, 6주령과 8주령 양군 간에는 통계 적으로 유의한 차이는 없었다(p<0.05). 결 론: 쥐 경동맥 손상 모델에서 생성되는 신생내막의 양을 이용 하는 연구에서 연구 목적에 맞는 적절한 주령 선택에 유의하 여야 하고, 주령과 체중의 밀집도를 높이는 것이 중요하다. 실험에 많이 사용되는 6~12주령(250~340 g)내에서 약간의 Jin-Sook Kwon, et al:Neointima Formation after Rat Carotid Injury·83 주령이나 체중 차이에도 예상보다 심한 신생내막의 차이가 있 으므로 특히 유의하여할 것으로 사료된다.

Perivascular Delivery of Rapamycin in Pluronic Gel Inhibits Neointimal Hyperplasia in a Rat Carotid Artery Injury Model, and the Complementary Role of Carotid Arteriography

정미진,박노관,김유경,심태진,정일하,배장환,황경국,김동운,조명찬,권진숙 대한심장학회 2008 Korean Circulation Journal Vol.38 No.2

Background and Objectives : Rapamycin has ben shown to inhibit the vascular smoth muscle cel migration and proliferation that contributes to neointimal formation. We investigated whether the perivascular delivery of rapa-of carotid arteriography. Materials and Methods : To assess the kinetics of rapamycins release from Pluronic gel, a [3H] thymidine incorporation asay was performed with using the media exposed to rapamycin in Pluronic gel for 10, 20, 60 and 120 min. We aplied 10 μg of rapamycin in Pluronic gel to the perivascular space of the carotid artery after the balloon injury (n=9), whereas only gel was applied in a control group (n=9). We performed the carotid arteriogra-phy and the morphometric analysis 14 days after injury. Results: The [3H] thymidine incorporation asay showed a reduction of uptake in a time-dependent manner (86%, 48%, 45% and 40% of the control, respectively, at 10, 20, 60 and 120 minutes). The inhibiting efect of rapamycin on neointimal hyperplasia was identified on the carotid arte-riography (mean luminal diameter; 0.75± 0.1 vs. 0.60± 0.12 arbitrary units, respectively, p<0.05) and on the morphometric analysis (neointima area: 0.09± 0.03 vs. 0.17± 0.06 mm2, respectively, p<0.05). Conclusion : This study demonstrated that perivascular delivery of rapamycin in Pluronic gel inhibits neointimal hyperplasia in a rat restenosis. In adition, this technique might be useful for vascular surgery such as coronary artery bypas grafting, arteriovenous fistula formation and peripheral vascular bypass graft insertion.

지중송전케이블 초기가압시 부분방전 검출을 위한 Compact형 현장 On-line PD 진단 시스템 개발 및 적용

백남열,최종혁,박노관,송호승,박수신,이전선 대한전기학회 2012 대한전기학회 학술대회 논문집 Vol.2012 No.7

고혈압-고지혈증 백서 모델에서 Lectin Like Oxidized Low Density

김기석,김송이,박노관,김동운,주승재,조명찬 대한고혈압학회 2011 Clinical Hypertension Vol.17 No.2

Background: Lectin-like, oxidized, low-density lipoprotein receptorreceptors (LOX-1) recognizes recognize vascular oxidized low-density lipoprotein (LDL), which may play an important role in the pathogenesis of atherosclerosis. We investigated the expressions expression of LOX-1 and redox-regulating thyoredoxinthioredoxin systems in a hypertension and hypercholesterolemia rat model. Methods: Spontaneously hypertensive rats (SHR) and Wistar-Kyoto rat (WKY) rats were fed with a normal cholesterol diet (NC) and a high cholesterol diet (HC) for 4 weeks. Plasma LDL cholesterol levels and blood pressure were measured at 1 and 4 weeks. Histological changes of atherosclerosis in the vessel was evaluated by hematoxylin and eosin staining and immunocytochemistry. The expressions expression of LOX-1 and thyoredoixnthioredoxin were measured by Western western blot analysis. Results: In the SHR groupsgroup, blood pressure after 4 weeks was significantly higher than initial levels. LDL-cholesterol levels in the SHR-HC group were increased at 4 weeks (15.3 ± 2.6 mg/dL vs. 20.2 ± 2.6 mg/dL, p < 0.01) compared with the SHR-NC group. In oxyblot analysis, the degree of oxidative stress of in the SHR-HC group was significantly higher than in the SHR-NC group (p < 0.05). The expressions expression of LOX-1 and Trx were was significantly increased in the SHR-HC group compared with the SHR-NC group (p < 0.05) on western blot analysis. Focal overexpressions overexpression of LOX-1 were was observed at the intima layer of the thoracic aorta, and was which wereonly observed in the SHR-HC group. Conclusions: The expressions expression of LOX-1 and oxidative stress were was significantly increased in the “hypertension with hypercholesterol” rat model. These findings suggested suggest that LOX-1 and redox systems may play a certain role in development and progression of atherosclerosis.

압축 공기를 이용한 Hot-Spot구간 온도상승 억제를 위한 국부냉각시스템 개발 적용

박수신,최종혁,박노관,송호승,백남열,김익생 대한전기학회 2012 대한전기학회 학술대회 논문집 Vol.2012 No.7