고분자 담체를 이용한 성체 간엽 줄기세포의 연골분화

박기숙,김은정,한창환,이일우,이해방,강길선 한국생체재료학회 2003 생체재료학회지 Vol.7 No.2

Articular cartilage exhibits little intrinsic repair capacity, and new tissue engineering approaches are being developed to promote cartilage regeneration using cellular therapies. Bone marrow-derived mesenchymal stem cells (MSCs) are highly proliferative and multipotential cells that have been considered as ideal candidate cells for tissue engineering applications. In this study, we have characterized the chondrogenic potential of rabbit MSCs in porous polymeric scaffolds by poly(lactic acid-co-glycolic acid) (PLGA), poly(glycolic acid) (PGA) as three-dimensional constructs to support chondrogenic differentiation following transforming growth factor-1 (TGF-1 treatment. Rabbit MSCs were isolated by percoll gradient and adherent cell cultures were established. The cells were then passaged into an aggregate culture system in a defined medium. The defined medium included dexamethason and TGF-1, cells secreted an extracellular matrix (ECM) incorporating type II collagen, aggercan, and proteoglycans. In vitro, MSCs were seeded in polymeric scaffolds for 28 days. The chondrogenesis of MSCs-seeded polymer scaffolds culture was assessed by histology and RT-PCR for chondrogenic phenotype. In histological analysis from safranin-O staining, we confirmed that chondrogenic differentiated rabbit MSCs expressed chondrocyte-like morphologies. Also we observed that type II collagen expressed by RT-PCR. In conclusion, this study demonstrated that the biodegradable porous polymeric scaffolds were able to provide three-dimensional constructs for inducing chondrogenic differentiation of rabbit MSCs.

생약(한약)제제의 허가심사 기준 및 개선방향

박기숙 한국에프디시규제과학회(구 한국에프디시법제학회) 2011 한국에프디시법제학회 학술대회 Vol.2011 No.1

現代中國語方位詞의 空間系統硏究

박기숙 중국어문논역학회 1999 中國語文論譯叢刊 Vol.4 No.-

소의 간 미크로좀 Aldehyde Dehydrogenase의 정제 및 특성에 관한 연구

박기숙,주충노,Park, Ki-Sook,Joo, Chung-No 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5

소의 간 미크로좀 분획으로부터 aldehyde dehydrogenase[ALDH, EC 1.2.1.3]를 sodium deoxycholate 처리, DEAE-Sephacel, Blue Sepharose CL-6B, 그리고 5'-AMP Sepharose 4B affinity 등의 column chromatography 방법으로 186배 정제하였다. Sephacryl S-200 gel filtration 방법으로 측정된 이 효소의 분자량은 293.9KDa이었고 SDS-polyacrylamide gel 전기영동법을 수행한 결과 subunit 분자량이 54.9KDa인 homotetramer임이 판명되었다. 최적 pH 범위는 9.0~9.5로 비교적 넓었으며 $25^{\circ}C$에서는 안정하지만 $37^{\circ}C$에서 10분간 방치하면 효소활성이 44% 감소되었다. 탄소수가 짧은 aliphatic aldehydes나 aromatic aldehyde에 대한 $K_m$값은 $10^{-3}$ M 수준이었으나 탄소수가 긴 aliphatic aldehyde($C_7{\sim}C_9$)에 대한 $K_m$값은 $10^{-6}$ M 수준으로 아주 낮은 것으로 보아 미크로좀 ALDH가 미크로좀 막에서 lipid peroxidation과정에서 생성되는 fatty aldehydes의 산화에 중요한 역할을 할 것으로 생각된다. 또한 이 효소는 nonanal을 기질로 사용했을 경우에만 속도-기질농도 곡선이 sigmoidal saturation curve를 보였고 modified Lineweaver-Burk Plot과 Hill Plot을 행한 결과 결합자리수가 4임이 밝혀졌다. Nonanal의 $[S]_{0.5}$는 $18.9{\times}10^{-6}M$이었고 nonanal의 농도가 증가하면 효소활성이 크게 증가됨을 알 수 있었다. 이 효소의 활성 부위의 아미노산 잔기를 조사하기 위하여 sulfhydryl기에 특이하게 반응하는 NEM, DNTB를 처리했을 때는 효소의 활성이 부분적으로 억제되었으나 hydroxyl기에 특이하게 반응하는 PMSF를 처리했을 경우 효소활성의 억제 정도가 매우 낮은 것으로 보아 효소활성 부위에 sulfhydryl기가 존재할 것으로 생각된다. 또한 ALDH에 대한 inhibitor로 알려진 disulfiram에 대해서도 매우 민감하게 저해되지만 0.2mM의 높은 disulfiram 농도에서도 26%의 효소활성이 남아 있는 것으로 보아 disulfiram 작용 부위와 효소활성 부위는 동일하지 않은 것으로 생각된다. Bovine liver microsomal aldehyde dehydrogenase (ALDH) was purified 180 fold by DEAE-Sephacel, Blue Sepharose CL-6B, and 5'-AMP Sepharose affinity column chromatography. The moleculer weight of the native enzyme was determined to be 234,000 dalton by Sephacryl S-200 gel filtration. Analysis of the purified enzyme on SDS-polyacrylamide gel electrophoresis showed that it was composed of four identical subunits and the molecular weight of a subunit was 54,900 dalton. Optimum pH of the enzyme was found to be 9.0~9.5 and it was stable at $25^{\circ}C$ but the activity was decreased as much as 44 percent after incubation at $37^{\circ}C$ for 10 min. The $K_m$ values for short chain aliphatic aldehydes and aromatic aldehyde were $10^{-3}$ M level but the $K_m$ values for long chain aliphatic aldehydes ($C_7{\sim}C_9$) were relatively low ($10^{-6}$M level). These results suggest that the microsomal aldehyde dehydrogenase may play an important role in the oxidation of fatty aldehydes which can be produced during the lipid peroxidation whithin the microsomal membranes. The enzyme showed a sigmoidal saturation curve when nonanal was used as a substrate and the number of the binding sites was determined to be 4 by modified Lineweaver-Burk Plot and Hill Plot. $[S]_{0.5}$ was found to be $18.9{\times}10^{-6}M$. These results suggest that the enzyme might be stimulated when the concentration of nonanal increases.

스펜서 테크닉이 유착성 관절낭염 환자의 어깨관절 가동범위와 통증, 기능에 미치는 영향

박기숙,정기용,park, Ki-suk,Jeong, Ki-yong 대한정형도수물리치료학회 2018 대한정형도수물리치료학회지 Vol.24 No.2

Background: The Purpose of this study was to evaluate the value of Spencer technique on the range of motion (ROM), Pain, function in patients with shoulder adhesive capsulitis. Methods: subjects consisted of 30patients who were diagnosed shoulder adhesive capsulitis. All subjects are randomly assigned to 2groups: Spencer technique (ST) group (n=15), self assistive ROM exercise(S-A ROM E) group (n=15). The subjects performed an intervention program 30 minuets per day and was repeated 3 times a week for 4 weeks a total of 12 times. ROM of flexion, abduction, external rotation, internal rotation were measured using a goniometer. The visual analog scale (VAS), Shoulder pain and disability index (SPADI) were used to measure pain, functional ability. Results: In the intergroup comparisons after the intervention, ROM of flexion, abduction, internal rotation, VAS, SPADI were significantly different(p<.05). Spencer technique was more effective for improving ROM, pain, functional ability than self assistive ROM exercise. Conclusions: Our study suggest that considering Spencer technique for the patient with shoulder adhesive capsulitis. Further studies on Spencer technique are needed in the future.

소의 간 미크로좀 Aldehyde Dehydrogenase 의 정제 및 특성에 관한 연구

박기숙,주충노 ( Ki Sook Park,Chung No Joo ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.5

Bovine liver microsomal aldehyde dehydrogenase (ALDH) was purified 180 fold by DEAF-Sephacel, Blue Sepharose CL-6B, and 5`-AMP Sepharose affinity column chromatography. The molecules weight of the native enzyme was determined to be 234,000 dalton by Sephacryl S-200 gel filtration. Analysis of the purified enzyme on SDS-polyacrylamide gel electrophoresis showed that it was composed of four identical subunits and the molecular weight of a subunit was 54,900 dalton. Optimum pH of the enzyme was found to be 9.0∼9.5 and it was stable at 25℃ but the activity was decreased as much as 44 percent after incubation at 37℃ for 10 min. The K_m values for short chain aliphatic aldehydes and aromatic aldehyde were 10^(-3) M level but the K_m values for long chain aliphatic aldehydes (C_7∼C_9) were relatively low (10^(-6) M level). These results suggest that the microsomal aldehyde dehydrogenase may play an important role in the oxidation of fatty aldehydes which can be produced during the lipid peroxidation whithin the microsomal membranes. The enzyme showed a sigmoidal saturation curve when nonanal was used as a substrate and the number of the binding sites was determined to be 4 by modified Lineweaver-Burk Plot and Hill Plot. [S]_(0.5) was found to be 18.9 × 10^(-6) M. These results suggest that the enzyme might be stimulated when the concentration of nonanal increases.

SIS Sponge as Potential Cell Carriers for Neurotransplantation

박기숙,하현정,이일우,김문석,이해방,강길선 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.4

중국어의 <모친(母親)> 명칭에 대한 고찰

박기숙 한국어내용학회 1996 한국어내용론 Vol.4 No.-

랫드와 마우스에서 폴리(아크릴산소다-아크릴산)의 급성 경구 독성 시험에 관한 연구

박기숙,서경원,김효정 한국식품위생안전성학회 1993 한국식품위생안전성학회지 Vol.8 No.2

p21Cip/WAF1 activation is an important factor for the ERKpathway dependent anti-proliferation of colorectal cancer cells

박기숙,전성후,오종원,최강열 생화학분자생물학회 2004 Experimental and molecular medicine Vol.36 No.6

p21Cip/WAF1, an important regulator of cell proli-feration, is induced by both p53- and extra-celular signal regulated kinase (ERK) pathways. The induction of p21Cip/WAF1 occurs by prolonged activation of the ERKs caused by extracellular stimuli, such as zinc. However, not all the cells appeared to respond to ERK pathway dependent p21Cip/WAF1 induction. Here we investigated the cause of such diference using colorectal cancer cells. p21Cip/WAF1 induction and concomitant reduc-tion of bromodeoxyuridine (BrdU) incorporation DLD-1. However, HCT-16 cels with high end-ogenous p21Cip/WAF1 levels did not show any ad-ditional increment of p21Cip/WAF1 levels by zinc treatment and did maintain high BrdU incor-poration level. The p21Cip/WAF1 induction by zinc depended upon prolonged activation of extra-cellular signal regulated kinase (ERK) was not ob-served in HCT-116 cells. The percentage of BrdU positive cells was 50% higher in p21Cip/WAF1 -/- HCT-116 cells compared to p21Cip/WAF1 +/+ HCT- 16 cels, and no cels induced p21Cip/WAF1 in-corporated BrdU in its nucleus, yet confirming the importance of p21Cip/WAF1 induction in anti- proliferation. These results again suport that p21Cip/WAF1 induction is a determinant in the re-gulation of colonic proliferation by the ERK pathway.