β-Lactamase (VPA0477) 유전자를 표적으로 Polymerase chain reaction에 의한 장염비브리오( Vibrio parahaemolyticus )의 검출

박권삼 ( Kwon Sam Park ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.6

In this study, the β-lactamase (VPA0477) gene was used as a new target for the PCR-based detection ofVibrioparahaemolyticus . Primers specific for the β-lactamase (VPA0477) gene of V. parahaemolyticus , were designed and incorporated into a PCR-based assay. The assay was able to specifically detect all of the 191 V. parahaemolyticus strains tested, but did not result in amplification of 39 other Vibrio spp. and nonVibrio spp. strains tested. The detection limit of the assay was 10 CFU of V. parahaemolyticus RIMD2210633 from pure culture broth. The β-lactamase (VPA0477) gene-based assay developed in this study was sensitive and specific, and has great potential for the accurate detection and identification of V. parahaemolyticus in seawater or seafood samples.

고농도 에탄올내성 초산균의 개발 및 배양특성

박권삼(Kwon-Sam Park),장동석(Dong-Suck Chang),조학래(Hak-Rae Cho),박욱연(Uk-Yeon Park) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.4

식초 생산의 수율을 높이기 위해 재래식 식초생산에 사용되고 있는 종초로 부터 고농도의 에탄올에 내성을 나타내는 균주를 에탄올 순양법으로 개발해 내었다. 에탄올이 5~10% 첨가된 액체배지에 종초를 각각 가하여 에탄올 10%에서 증식이 가능한 균주를 순양 개시 42일만에 분리해 내었고, 이를 동정하여 Acetobacter sp. FM-10이라고 명명하였으며, 이 균주의 배양 특성을 살펴본 결과는 다음과 같다. Acetobacter sp. FM-10의 중식 최적조건을 살펴본 결과, 최적온도는 30℃였으며, 최적 pH는 5.0이었다. 초산생산에 적합한 배지의 조성은 에탄올 10%, 포도당 5%, yeast extract 1%가 함유된 배지였다. 식초생산 최적조건은 에탄올이 10% 첨가된 배지를 이용하여 30℃에서 정치배양시 배양 20일만에 최고 산도인 9.0%에 도달하였다. To increase the yield of acetic acid production, the author developed the bacterial strain which could grow well in high concentration of ethanol from the seed culture using in conventional vinegar production factory. By attenuation of the isolated strain in the broth media containing 5-10% ethanol, we could get the strain which could grow in the broth medium containing 10% ethanol. This strain was identified and named as Acetobacter sp. FM-10, and it's cultural characteristics were also investigated. The medium containing 10% ethanol, 5% glucose and 1% yeast extract was suitable for the acetic acid production with Acetobacter sp. FM-10. Optimum temperature and pH for the growth of Acetobacter sp. FM-10 were 30℃ and 5.0, respectively. The acidity of culture medium was reached to 9.0% after 20 days static cultivation at 30℃.

시판 젓갈에서 분리한 Bacillus cereus의 독소 유전자 및 항균제 내성 분석

박권삼 ( Kwon-sam Park ),조의동 ( Eui-dong Cho ),김희대 ( Hee-dai Kim ) 한국수산과학회(구 한국수산학회) 2020 한국수산과학회지 Vol.53 No.6

Twenty-three Bacillus cereus strain isolated from commercial jeotgal were investigated for 11 toxin genes and susceptibility to 25 different antimicrobials. The hemolytic enterotoxins hblA, hblC, and hblD were detected in 13.0%, and non-hemolytic enterotoxins nheA, nheB, and nheC were detected in 26.1%, 100%, and 100% of the isolates, respectively. The positive rates of cytK, entFM, becT, hlyII, and ces were 73.9%, 60.9%, 26.1%, 8.7%, and 0.0%, respectively. According to the disk diffusion susceptibility test, all of the strains studied were resistant to cefuroxime, followed by cefoxitin (78.3%), oxacillin (78.3%), ampicillin (69.6%), penicillin G (69.6%), and amoxicillin (65.2%). However, all the strains were susceptible to 11 other antimicrobials, including amikacin, chloramphenicol, and ciprofloxacin. The average minimum inhibitory concentrations of amoxicillin, ampicillin, and cefuroxime against B. cereus were 462.9, 235.0, and 135.0 μg/mL, respectively. These results highlight the need for sanitizing commercial jeotgal, and provide evidence to help reduce the risk of jeotgal contamination by antimicrobial-resistant bacteria.

고농도 에탄올 내성균 Acetobacter sp. FM - 10을 이용한 초산 발효조건 검토

박권삼(Kwon-Sam Park),이명숙(Myung-Suk Lee),목종수(Jong-Soo Mok),장동석(Dong-Suck Chang) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.5

10%의 고농도 에탄올에 내성을 나타내는 Acetobacter sp. FM-10의 초산 발효조건을 검토한 결과, Acetobacter sp. FM-10을 이용하여 정치배양했을 경우, 배양액의 초기 초산농도는 초산 생성속도에 큰 영향을 미쳤는데, 0.75% 농도 까지는 초기 초산농도가 높을수록 좋았으나, 1% 부터는 초산생성이 현저히 저해되었다. 한편, 배지의 초기 에탄올 농도를 5%로 하여 배양하다가 배양액속의 에탄올이 소모된 시점에서 에탄올을 1%씩 추가시켜 주었을 경우에 배양 초기 부터 10%의 에탄올배지에서 배양시킨 경우 보다 발효수율이 월등히 저하되어 주목할만한 산도 증가는 나타나지 않았다. 100rpm 이상으로 진탕배양하였을 때 진탕속도가 증가할수록 최고 산도에 도달하는 시간이 빨라졌으며, 150rpm에서는 배양 10일 만에 최고 산도가 8.3%에 도달하여 정치배양 보다 최고산도에 이르는 시간이 10일 정도 빨랐다. 발효조에서 초산생산 최적조건은 교반속도 700rpm, 통기량 5L/min였다. The fermenting conditions for acetic acid production with Acetobacter sp. FM-10 which could grow in the medium containing 10% ethanol were investigated. Initial concentration of acetic acid in broth medium affected greatly to the fermentation speed. For example, the acetic acid production increased proportionally by the increasing of initial concentration of acetic acid up to 0.75%, while it decreased rapidly in case of the acetic acid concentration was higher than 1.0%. When the cultivation was started with broth medium containing 5% ethanol, the additional adding ethanol during the fermentation was not significantly increased the acidity of the medium. The acidity of the medium containing 10% ethanol was reached to 8.3% after shaking cultivation for 10 days with 150rpm shaking speed. Acetic acid production with shaking cultivation was faster than static cultivation by about 10 days under the same condition except shaking. In acetic acid fermentation with the batch style fermentor, the optimum fermentation condition was 700rpm of agitation speed and 5L/min of air flow rate in 3L culture medium.

해수 및 수산물에서 분리한 Vibrio vulnificus의 병원성 유전자 및 항균제 내성

박권삼 ( Kwon-sam Park ),조의동 ( Eui-dong Cho ),김희대 ( Hee-dai Kim ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.6

We isolated 28 Vibrio vulnificus strains from seawater and fisheries and investigated the positive rate of eight virulence genes. Additionally, we evaluated the susceptibility of these strains to 25 antimicrobials. The positive rates of fur, vvhA, tcp, rtxA, vcgC, viuB, vvp, and acfA were 100, 92.9, 92.9, 67.9, 64.3, 25.0, 14.3, and 7.1%, respectively. A disk diffusion susceptibility test revealed that, all the investigated strains had the highest resistance to amoxicillin and oxacillin, followed by that to streptomycin (96.4%), cefoxitin (92.9%), clindamycin (82.1%), amikacin (67.9%), vancomycin (46.4%), nalidixic acid (7.1%), penicillin G (7.1%), and ampicillin (3.6%). Moreover, they were susceptible to 10 other antimicrobials, including cefotaxime, chloramphenicol, erythromycin, gentamicin, and rifampicin. Notably, amoxicillin, oxacillin, and streptomycin had average minimum inhibitory concentrations of 132.6, 603.4, and 23.1 μg/mL against V. vulnificus, respectively. These observations provide new insights regarding the necessity for sanitation of commercial fisheries and can potentially, help reduce the risk posed by fisheries contaminated with bacteria resistant to antimicrobials.

장염비브리오의 biofilm 형성에 영향을 미치는 인자

노아름 ( A Reum No ),박권삼 ( Kwon Sam Park ) 한국수산과학회 2009 한국수산과학회지 Vol.42 No.5

Entrapped Mixed Microbial Cell (EMMC) 공정을 이용한 수산물 가공 폐수처리에서 유기물 및 질소 동시제거

정병철 ( Byung Cheol Jeong ),박권삼 ( Kwon Sam Park ),정병곤 ( Byung Gon Jeong ) 한국물환경학회 ( 구 한국수질보전학회 ) 2006 한국물환경학회지 Vol.22 No.3

Feasibility of simultaneous removal of organic materials and nitrogen in the wastewater from fisheries processing plant was evaluated using entrapped mixed microbial cell (EMMC) process. The experiment was performed using activated sludge from municipal sewage treatment plant which was immobilized with gel matrix by cellulose triacetate. It was found the stable operation at the treatment system which is composed of anoxic and oxic tank, was possible when the organic and nitrogen loading rates were increased stepwise. The organic and nitrogen loading rates were conducted from 0.65 to 1.72 ㎏COD/m3/d and from 0.119 to 0.317 ㎏ T-N/m3/d with four steps, respectively. The maximum nitrogen loading rate which could satisfy the regulated effluent standard of nitrogen concentration, was 0.3 ㎏ T-N/m3/d. The removal efficiency of total nitrogen was decreased apparently as increasing nitrogen loading rates, whereas the removal efficiency of ammonium nitrogen was effective at the all tested nitrogen loading rates. Therefore, it was concluded that nitrification was efficient at the system. Nitrate was removed efficiently at the anoxic tank. whereas the nitrification efficiency at the oxic tank ranged 94.0% to 96.9% at the tested loading rates. The removal efficiencies of chemical oxygen demand (COD) and those of total nitrogen at the entire system ranged from 94.2% to 96.6% and 73.4% to 83.4%, respectively.

시판 까나리(Ammodytes personatus) 액젓의 biogenic amines 함량

엄인선 ( In Seon Um ),박권삼 ( Kwon Sam Park ) 한국수산과학회 2015 한국수산과학회지 Vol.48 No.6

This study investigated the quality of seven commercial salted and fermented sand lance Ammodytes personatus sauces by measuring their chemical compositions, bacteria concentrations, and biogenic amine contents. The sauces had a 63.97-67.32% (mean: 65.72%) moisture content, 23.16-24.03% (mean: 23.60%) salinity, 5.51-6.14 (mean: 5.81) pH, 164.76-217.98 mg/100 g (mean: 182.97 mg/100 g) volatile basic nitrogen, 0.94-1.42% (mean: 1.24%) total nitrogen, and 673.77-791.86 mg/100 g (mean: 720.76 mg/100 g) amino nitrogen content. Viable cell counts ranged from 1.2×101 to 2.9×102 CFU/mL, and number of biogenic amine-forming bacteria was very low or not detected. Overall, the tested samples had average levels of histamine of 301.02 mg/kg, cadaverine of 29.78 mg/kg, tyramine of 199.21 mg/kg, putrescine of 183.00 mg/kg, and tyramine of 13.01 mg/kg. This strongly suggests that it is necessary to monitor the biogenic amine contents of commercial salted and fermented sand lance sauces carefully to ensure consumer health.

MPN 및 H-NS 유전자를 표적으로 하는 PCR assay를 병용한 장염비브리오(Vibrio parahaemolyticus)의 정량

김태옥 ( Tae Ok Kim ),박권삼 ( Kwon Sam Park ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.5

We applied a combination of most probable number-polymerase chain reaction (MPN-PCR) methods using a PCR procedure targeting the H-NS (VP1133) gene to detect Vibrio parahaemolyticus presence and density in seawater as well as within short-necked clam Ruditapes philippinarum tissues collected from Gomso Bay, Korea. In 30 seawater samples, V. parahaemolyticus levels ranged from less than 1.8 to 1.1×10³ MPN/100 mL, and samples from August showed higher than those from other months. Furthermore, the levels of V. parahaemolyticus in six short-necked clam samples ranged from 7.8×10² to 2.1×10³ MPN/100 g, approximately 2.5 times higher than in seawater samples from the corresponding month. Our results provide data on V. parahaemolyticus contamination in seawater and short-necked clam tissues, and help to improve quantitative methods of assessing V. parahaemolytcius levels.

모시잎의 항산화 효과 및 암세포주에 대한 세포 독성

김인숙(In-Sook Kim),박권삼(Kwon-Sam Park),유현희(Hyeon-Hee Yu),신미경(Mee-Kyung Shin) 동아시아식생활학회 2009 동아시아식생활학회지 Vol.19 No.3