체외순환기 인터루킨-6의 생성과 염증반응간의 상관관계에 관한 연구

박광훈,최석철,한일용,최국렬,최강주,조광현 대한흉부심장혈관외과학회 2000 Journal of Chest Surgery Vol.33 No.5

Background: With open heart surgery(OHS), it has been recognized that many postoperative complications and postperfusion syndrome are associated with the activations of complements and leulocytes. Recently, some investigators also demonstrated that interlukin-6(IL-6) linked highly with postperfusion syndrome. The puropose of this study was to investigate the sequential changes of the IL-6 and to clarify each IL-6 relationship to the complements(C3, C4) and inflammatory response following cardiopulmonary bypass(CPB). Material and Method: To determine serum levels of IL-6, complements, leukocytes, and biochemistric markers of liver and renal function, blood samples were taken from th radial artery in 30 adult patients undergoing OHS with CPB. Result: Serum IL-6 levels incrased significantly at 10 minutes after CPB-on(CPB-10) in comparison with the control levels and reached the peak at CPB-off(p<0.05). Serum complement levels declined rapidly at CPB-10 and remained at the lower levels during CPB(p<0.01). Sequential changes of IL-6 levels had positive correlations with the changes of total leukocytes and neutrophil fractions(p<0.05), but had negative correlations with lymphocyte fractions(p<0.05). Changes of C3 related postively to monocyte fractions(p<0.05). Postoperative levels of total protein and albumin, decreased significantly in comparison with the control levels(p<0.01), while the postoperative levels of AST(aspartate transaminase) and bilirubin increased (p<0.01). At CPB-off, IL-6 levels had negative correlations with total protein and albumin levels(r=-0.60, -0.47 respectively, p<0.05), whereas C3 levels had positive correlations with albumin levels(r=0.40, p<0.05). IL-6 levels, as well as neutrophil fractions, had positive correlations with aortic clamp time(ACT) and total bypass time(TBT) (IL-6; r=0.82, 0.79 respectively, neutrophil fractions; r=0.50, 0.56 respectively, p<0.05), wheres lymphocyte frations and albumin levels had negative correlations whith ACT and TBT(lymphocyte fractions; r=-0.52, -0.58 respectively, albumin; r=-0.58, -0.55 respectively, p<0.05). Conclusion: These data showed that elevated production of serum IL-6 during CPB may play a pivotal role in systemic inflammatory responses and prologed CPB period may be assosiated with more sever postperfusion syndromes.

H.264 기반 선택적인 미세입자 스케일러블 코딩 방법

박광훈,유원혁,김규헌 한국정보과학회 2004 정보과학회 컴퓨팅의 실제 논문지 Vol.6 No.2

본 논문에서는 선택적으로 강화계층의 시간적 예측 정보를 사용하는 H.264 기반 선택적인 미 세입자 스케일러블 비디오 코딩 방법을 제안한다. 제안된 방법의 기본계층은 최근 표준화되었으며 고압축률이 특징인 H.264 (MPEG-4 Part 10 AVC) 알고리즘으로 코딩한다. 강화계층은 기본적으로 국제표준인 비트플레인 기반 MPEG-4 (Part 2) 미세입자스케일러블 코딩 방법으로 코딩한다. 본 논문에서는 엔코더측에서 강화계층간 시간적 예측 방법에 의해 발생된 효과적인 영상 정보를 드리프트 현상이 최소화하는 방향으로 선택적으로 적용하여 디코더측에 전송하는 방안을 제안하였다. 강화계층간 예측 방법만을 추가했을 때는 시간적 중복성을 줄여주는 효과를 볼 수 있지만 저비트율 대역에서 엔코더와 디코더간의 참조 저장 영상 불일치로 인한 드리프트 현상이 심하게 발생한다. 제안된 알고리즘은 시간적 예측 결과가 우수하여 코딩효율을 현저히 높혀줄 수 있는 경우에만 선택적으로 강화계층간의 시간적 예측 정보를 사용하였으며, 이로 인하여 저비트율 대역에서의 드리프트 현상을 현저하게 줄 일수 있었으며, 전반적으로 코딩 효율을 높여주는 효과를 가져왔다. 여러 영상 시퀀스를 대상으로 실험한 결과, 제안된 코딩 방법은 현존하는 국제표준인 MPEG-4 기반 미세입자 스케일러블 코딩 방법보다 같은 비트율 대역에서 영상화질이 약 3∼5 dB 높은 성능을 보여주고 있으며, H.264를 기반으로한 미세입자 스케일러블 코딩 방법보다도 약 1∼3 dB 높은 성능을 보여주고 있음을 발견할 수 있었다. This paper proposes the H.264-based selective fine granular scalable (FGS) coding scheme that selectively uses the temporal prediction data in the enhancement layer. The base layer of the proposed scheme is basically coded by the H.264 (MPEG-4 Part 10 AVC) visual coding scheme that is the state-of-art in codig efficiency. The enhancement layer is basically coded by the same bitplane-based algorithm of the MPEG-4 (Part 2) fine granular scalable coding scheme. In this paper, we introduce a new algorithm that uses the temproal prediction mechanism inside the enhancement layer and the effective selection mechanism to decide whether the temporally-predicted data would be sent to the decoder or not. Whenever applying the temporal prediction inside the enhancement layer, the temporal redundancies may be effectively reduced, however the drift problem would be severly occurred especially at the low bitrate transmission, due to the mismatch bewteen the encoder's and decoder's reference frame images. Proposed algorithm selectively uses the temporal-prediction data inside the enhancement layer only in case those data could siginificantly reduce the temporal redundancies, to minimize the drift error and thus to improve the overall coding efficiency. Simulation results, based on several test image sequences, show that the proposed scheme has 1∼3 dB higher coding efficiency than the H.264-based FGS coding scheme, even 3∼5 dB higher coding efficiency than the MPEG-4 FGS international standard.

담수산 거머리의 분류학적연구

박광훈,김종환 충남대학교 자연과학연구소 1989 忠南科學硏究誌 Vol.16 No.1

The freshwater leeches were collected from several localities in Korea during April 1987-October 1988, and identified them based on the morphological features. Forteen species of leeches were identified and they were grouped into 12 genera, 4 families and 2 orders. Ten species of them were newly recorded in the Korean leech founa.known a Alboglossiphonia lata was known of snail predator leeches and most abundant among the species. The taxonomic list of freshwater leeches is as follows: 1.^*Theromyzon tessulatum(O.F.Mu‥ller), 2. Torix tagoi(Oka), 3.^* Torix orientalis(Oka), 4.^*Kelobdella striata(Ringuelet), 5.^* Parabdella quadrioculata(Moore), 6.^* Glossiphonia complanata(Linnaeus), 7. Alboglossiphonia heterodlita(Linneus), 8.^* Alboglossiphonia lata(Oka), 9.^* Batracobdella paludosa(Carena), 10.^* Kimnotrachelobdella sinensis(Blanchared), 11.^* Barbronia weberi Blanchard, 12.^* Erpobdella lineata(O.F.Mu‥ller), 13. Hirudo nipponia Whitman, 14. Whitmania pigra(Whitman). Among them, 10 species with asterik are newly recorded from Korea.

포도 뿌리혹병 방제를 위한 길항균 선발

박광훈,김정희,차재순 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-

Previous study showed that crown gall disease is very severe on the tetraploid cultivars of grape. One of the promising control measures of the disease is biological control using a antagonist against pathogen, Agrobacterium vitis. Total 215 bacteria and 21 putative agrobacteria were isolated from galls of grapevine and a Korean wild grape, Vitis amurensis respectively. Antagonistic ability of the isolates was checked by growth inhibition test of pathogens, A. tumefaciens and A. vitis on agar and on tamato seedling by co-inoculation of the isolates and pathogen. Two selected antagonists, isolate 197 and Va 21 strongly suppressed gall formation of A. vitis K306 on grapevine when they were co-inoculated with pathogen K306. Both gall size and rate of gall formation were significantly lower on grapevines inoculated of 197-K306(9:1) or Va 21-K306(9:1) mixture than on them inoculated of just K306. Antagonistic ability of the two isolates was similar to F2/5, which is known as a strongest antagonist against A. vitis so far. Further field study for biological control of crown gall disease on grapevine by the two isolates is needed.

狹心症의 最新知見

朴光勳 한국의학사 1990 診斷과治療 Vol.10 No.3

기초 물리량 측정 장치

박광훈 한국소음진동공학회 2005 소음 진동 Vol.15 No.3

폐과오종(肺過誤腫)의 일치험례(一治驗例)

박광훈,지정희,Park, Gwang-Hun,Ji, Jeong-Hui 대한흉부심장혈관외과학회 1976 Journal of Chest Surgery (J Chest Surg) Vol.9 No.2

Since. its first description by Albrecht in 1904 that hamartoma (Greek. meaning "error" and "tumor")is tumor-like malformation characterized by abnormal mixture of normal constituent in any organ it arises, hamartoma involving the lung has assumed the following clinical features.: 1) all the pulmonary lesions presenting as "coin lesions", it occupies about 8% according to various reports. It also constitutes the most prevalent lesion of all the benign lung tumors, appearing in 0.25% of the general population. 2) Peak incidence of the lesion occurs in the 5th and 6th decade as the carcinoma patient do, and the occasional growing tendency and periperal location as in this presentation renders the differential diagnosis from the malignant disease difficult and surgical intervention inevitable for definite histological diagnosis and treatment. 3) Nearly all the clinical investigations such as history, physical exam, bronchoscopy, culture, and cytological exam of sputum sre likely to futile:the only tool for detecting the presence of the lesion is X-ray, butthat's short of telling the true nature of it. 4) Because of its age of presentation and growing tendency in middle-aged adult, it still controversial whether it is developmental anomaly as Albrecht described, or true neoplasm denovo of fibrous connective tissue origin. This is a case report of surgical experience of pulmonary hamartoma which had nearly all the typical clinical features above mentioned and was reviewed with related literatures.

Kahweol from Coffee Induces Apoptosis by Upregulating Activating Transcription Factor 3 in Human Colorectal Cancer Cells

박광훈,송훈민,정진부 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.3

Kahweol as a coffee-specific diterpene has been reported to induce apoptosis in human cancer cells. Although some molecular targets for kahweol-mediated apoptosis have been elucidated, the further mechanism for apoptotic effect of kahweol is not known. Activating transcription factor 3 (ATF3) has been reported to be associated with apoptosis in colorectal cancer. The present study was performed to investigate the molecular mechanism by which kahweol stimulates ATF3 expression and apoptosis in human colorectal cancer cells. Kahweol increased apoptosis in human colorectal cancer cells. It also increased ATF3 expression through the transcriptional activity. The responsible cis-element for ATF3 transcriptional activation by kahweol was CREB located between -147 to -85 of ATF3 promoter. ATF3 overexpression increased kahweol-mediated cleaved PARP, while ATF3 knockdown attenuated the cleavage of PARP by kahweol. Inhibition of ERK1/2 and GSK3β blocked kahweol-mediated ATF3 expression. The results suggest that kahweol induces apoptosis through ATF3-mediated pathway in human colorectal cancer cells.

Agrobacterium radiobacter K84에 의한 장미 뿌리혹병의 생물적 방제

박광훈,차재순 충북대학교 첨단원예기술개발연구센터 2000 연구보고서 Vol.5 No.-

최근 우리 나라의 여러 작물에서 뿌리혹병(根頭癌腫病, crown gall)의 발생이 증가하고 있음이 보고되고 있으며, 특히 중요한 화훼작물인 장미를 재배하는 시설하우스에서 뿌리혹병의 발생은 매우 심한 피해를 가져오고 있다. 그럼에도 불구하고 이 병에 대한 방제는 거의 이루어지지 않고 있는 실정이다. 본 연구에는 전 세계적으로 매우 성공적으로 뿌리혹병의 생물농약으로 개발된 Agrobacterium radiobacter K84의 장미 뿌리혹병 방제효과를 검정하여 국내에서 장미 뿌리혹병의 방제에 그 사용가능성을 확인하고자 하였다, 장미의 지상부에 인위적인 상처를 주고 병원균을 접종하기 전에 그리고 접종후 A. radiobacter K84 균주 현탁액의 살포는 뿌리혹병의 발병율과 혹의 크기를 크게 감소시켰다. K84균주를 처리한 장미에서 뿌리혹병의 발병율은 5.0 - 6.7%로서 병원균을 단독 접종한 대조구에서의 발병율 85% 비하여 크게 낮았다. K84 균주를 처리한 장미에서 형성된 혹의 크기와 생체중도 병원균만을 접종한 대조구 장미에 형성된 혹은 4-5%로서 K84균주의 처리에 의해 크게 감소하였다. 장미의 뿌리에 인위적인 상처를 주고 병원균의 접종 전 및 후에 K84 현탁액에 침지 처리한 처리에 의해서도 혹의 크기, 생체중, 그리고 병발생율이 크게 감소하였다. 전체적으로 K84균주 처리에 의한 지상부 혹의 생물적방제가는 92-94%이었고, 뿌리에서의 뿌리혹병 생물적방제가는 51-59%이었다. 이상의 결과는 A. radiobacter K84균주의 처리가 매우 효과적으로 장미 뿌리혹병을 방제할 수 있음을 보여주고 있다. Servere crown gall disease was occurred in green house cultivating rose in Jinchen, Chungbuk recently. Although it causes problem on rose cultivation, the growers do not have many choices of control measures for the disease now. A. radiobacter K84 has been known as a strong antagonist against A. tumefaciens, a pathogen causing crown gall disease and used as a biopesticide for crown gall in many countries since it had been introduced in 1972. We tested control ability of A. radiobacter K84 for the crown gall disease on rose. Spray of A. radiobacter K84 suspension on above ground of rose either before of after pathogen spray reduced size and fresh weight of galls significantly. Size and fresh weight of galls on roses inoculated with pathogen either before A. radiobacter K84 spray (pathogen-K84 treatment) or after A. radiobacter K84 spray (K84-pathogen treatment) were 4 to 5% of those of galls on roses inoculated pathogen only. Diseased rate of plants inoculated pathogen only was 85% whereas diseased rates of pathogen-K84 or K84-pathogen treatments were 6.7% and 5.0% respectively. Dipping of roots of rose in suspension of A. radiobacter K84 was also reduced size of galls and diseased rate significantly. These results indicate that A. radiobacter K84 is effective in the prevention of gall formation by A. tumefaciens and it can be used to control of crown gall disease of rose.

판매관리비를 중심으로 한 원가행태의 하방경직성에 관한 실증연구

박광훈 서울市立大學校 産業經營硏究所 2005 産經論集 Vol.20 No.1

판매관리비를 중심으로 한 원가행태의 하방경직성에 관한 실증연구