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민문희,김민지,최유진,유민주,김유라,안효빈,김채현,권채연,배성호,Min, Moon-Hee,Kim, Min-Ji,Choi, You-Jin,You, Min-Ju,Kim, Uy-Ra,An, Hyo-Bin,Kim, Chae-Hyun,Kwon, Chae-Yeon,Bae, Sung-Ho 한국미생물학회 2014 미생물학회지 Vol.50 No.2
Hrq1은 곰팡이 유전체에서 생물정보분석에 의해 발견된 새로운 RecQ helicase이다. 이 단백질은 인간의 RECQL4와 가장 상동성이 높으며 최근의 유전학적 생화학적 연구를 통해서 유전체 안정성을 유지하는데 어떤 역할을 할 것으로 예상되었다. 본 연구에서는 RECQL4와 상호작용하는 것으로 알려진 인간 유전자들과 상동성이 있는 효모 유전자들이 Hrq1과 상호작용하는지를 yeast two-hybrid assay를 이용하여 조사하였다. 총 11개의 유전자를 조사한 결과, nucleotide excision repair (NER) 인자 중의 하나인 Rad14이 Hrq1과 상호작용하는 것을 발견하였다. 또한 정제한 단백질을 이용한 pull-down assay로 Hrq1과 Rad14 사이의 직접적인 상호작용을 확인하였다. Hrq1과 Rad14 사이의 yeast two-hybrid 상호작용은 4-nitroquinoline-1-oxide에 의한 DNA 손상으로 더욱 증가하였으며, 이러한 상호작용의 증가는 또 다른 NER 인자인 Rad4에 의존적이었다. 이러한 결과들은 Hrq1이 Rad14과의 상호작용을 통하여 NER 과정에 어떤 역할을 할 가능성을 제시하고 있다. Hrq1 is a novel member of RecQ helicase family, found in fungal genomes by bioinformatics analyses. It is most homologous to human RECQL4 and recent genetic and biochemical studies suggested that it may play roles in the maintenance of genome stability. In this study, we investigated yeast two-hybrid interactions between Hrq1 and the yeast genes homologous to the human genes that are known to interact with RECQL4. Among the 11 genes tested, Rad14, a nucleotide excision repair (NER) factor, was found to interact with Hrq1. In addition, pull-down assay with the purified proteins revealed direct protein-protein interaction between Hrq1 and Rad14. The yeast two-hybrid interaction was enhanced by the DNA damage induced by 4-nitroquinoline-1-oxide, which was dependent on the presence of Rad4, a key NER factor. These results suggest that Hrq1 may function in NER through interaction with Rad14.
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민문희(Min Moon Hee),김수연(Kim Soo Yeon) 한국실내조경협회 2005 室內造景 = Journal of the Korean institute of interior Vol.7 No.2
Today it is needed to integrate the quantity and the quality of life for a balance of both human and environment. Those were to consider the aim of LOHAS, to research the behavior patterns and to suggest the ways to build space of consumption to recover sustainable value and the quality for life. The ecological theory of perception provides the human living, and meaning integrating norm to overcome the crisis caused by an eastern belief in human-centered wrong rationality formed at the past process of modernization. Prior to the overall consideration of ecological perception theory, looked into the concepts, contribution extent and its limitation of the existing perception theories for the environmental design. By experimentally applying inferred concepts of design to department bridge space and forming the space, certified the applicability of it to the green amenity space. The site is located in Nowon- Gu Sangge- Dong. The design was processed on the basis of survey, plants growth environment and plants characters. It is expected that this design would serve the commercial spaceconsumer as a symbolic, environmentally friendly space design.
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민문희(Min, Moon-Hee),서귀숙(Sus, Kuee-Sook) 한국실내디자인학회 2010 한국실내디자인학회 학술대회논문집 Vol.12 No.1
A This Study is about the characteristics of alley space in the urban residential area and old commercial area of Tokyo. The Alley Space plays an mediating role to connect the main space and the sub space in the urban, and also it provides a place to form community with nature. Especially, the alley space could expand urban space networking as that gives freely choice to choose pathway in the urban. The Alley Space has a restful effect and Inducing experiences that the space allows to the world.
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최도희,민문희,김민지,이리나,권성훈,배성호 한국미생물학회 2014 The journal of microbiology Vol.52 No.4
Hrq1 helicase is a novel member of the RecQ family. Amongthe five human RecQ helicases, Hrq1 is most homologous toRECQL4 and is conserved in fungal genomes. Recent geneticand biochemical studies have shown that it is a functionalgene, involved in the maintenance of genome stability. Tobetter define the roles of Hrq1 in yeast cells, we investigatedgenetic interactions between HRQ1 and several DNA repairgenes. Based on DNA damage sensitivities induced by 4-nitroquinoline-1-oxide (4-NQO) or cisplatin, RAD4 was foundto be epistatic to HRQ1. On the other hand, mutant strainsdefective in either homologous recombination (HR) or postreplicationrepair (PRR) became more sensitive by additionaldeletion of HRQ1, indicating that HRQ1 functions in theRAD4-dependent nucleotide excision repair (NER) pathwayindependent of HR or PRR. In support of this, yeast twohybridanalysis showed that Hrq1 interacted with Rad4,which was enhanced by DNA damage. Overexpression ofHrq1K318A helicase-deficient protein rendered mutant cellsmore sensitive to 4-NQO and cisplatin, suggesting thathelicase activity is required for the proper function of Hrq1in NER.
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