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근시(近視)의 침·지압 치료에 관한 고찰 - 한국어권, 영어권, 중국어권을 중심으로 -
문혜연,천진홍,김기봉,Moon, Hye Yeon,Cheon, Jin Hong,Kim, Ki Bong 대한한방소아과학회 2013 대한한방소아과학회지 Vol.27 No.4
Objectives The purpose of this study is to obtain knowledge and views on treatment of acupuncture and acupressure for myopia. Methods The key words such as 'myopia, acupuncture', 'myopia, acupressure', 'refractive errors, acupuncture', 'refractive errors, acupressure' are used for the research through PubMed. Total 13 studies among the searched 66 were analyzed, except duplicated studies and have little relation to myopia or acupuncture and acupressure. Results Total 13 studies were selected: RCT (5), NRCT (3), UCT (3), Review (2). RCT had two studies on treatment by stimulating auricular acupoints, one study on a treatment by stimulating auricular acupoints and meridian acupoints, one study on a treatment to stimulate auricular acupoints with medication, and one study on electroacupuncture. In NRCT, two studies were on treatment to stimulate auricular acupoints with medication and only one study was on analysis of effect according to intensity of acupuncture. UCT had two studies on acupuncture, and one study on treatment to stimulate auricular acupoints. In Review, there were one study on introduction of Chinese eye exercises, and one study on systematic review for effectiveness and safety of acupuncture. Conclusions The effect on treatment of acupuncture and acupressure for myopia had mostly positive results. However, still not many researches were done to conclude this found. Also, the design of the experiment should be more accurate with systematically designed experimental group and control group.
옥수수 뿌리로부터 분리한 Membrane - bound ATPase 의 특성에 관한 연구
문혜연(Hye Yeon Moon),노광수(Kwang Soo Roh),심웅섭(Woong Seop Sim) 한국식물학회 1981 Journal of Plant Biology Vol.24 No.4
The membrane-bound ATPases were separated on sucrose gradient from corn roots and characterized by pH optima, sensitivity to monovalent salt, Km and Vmax. The pH optima for the activity of all the ATPases associated with 13,000g pellet and 13,000∼80,000g pellet were 5 and 9, respectively. The ATPases in Fractions B and C of the 13,000g pellet were more active at pH 4 than pH 9. While, in the case of Fractions D, E and F, they were reverse. The activities of the ATPase in Fractions A and C of the 13,000∼80,000g pellet were greater at pH 5 than pH 9. On the other hand, the ATPases in Fractions B, D, E, and F were more active at pH 9 than pH 5. The optimum concentration of ATP for the assay was about 3 to 5mM. The Km`s for the membrane-bound ATPases in 13,000g pellet and in 13,000∼80,pppg pellet were 0.25mM. While Vmax values for 13,000g pellet were from 8.0 to 12.5 μM Pi/㎎ protein/hr, according to pH values, those for 13,000∼80,000g pellet were from 35.7 to 55.6 μM Pi/㎎ protein/hr. Activities of the membrane-bound ATPases in both 13,000g pellet and 13,000∼80,000g pellet were stimulated with increasing the concentration of K^+.
Effects of Cyclic AMP on the Activities of Ribosome and Elongation Factors EF-1 and EF-2
심웅섭,문혜연,Sim, Woong-Seop,Moon, Hye-Yeon 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.3
단백질의 생합성에 필요한 여러가지 components의 활성에 미치는 cAMP 의 효과를 조사하였다. cAMP로 처리된 ribosome의 활성은 대조구에 비하여 27% 증가하였으며 EF-1 과 EF-2의 활성은 cAMP에 의하며 각각 17%와 20% 증가 하였다. The effects of cAMP on the activities of the various components necessary for protein biosynthesis were investigated. 1. The rate of protein biosynthesis was increased about 27% by ribosomes treated with cAMP. 2. The activities of elongation factors EF-1 and EF-2 were considerably stimulated by exogenous cAMP.
Agrobacterium tumefaciens KU12로부터 Octopine형 Ti 및 잠재 플라스미드의 제거에 의한 숙주 개발
하운환,이용욱,문혜연,심웅섭,Ha, Un-Hwan,Lee, Yong-Woog,Moon, Hye-Yeon,Sim, Woong-Seop 한국미생물학회 1994 미생물학회지 Vol.32 No.1
Agrobacterium tumefaciens KU12내에 존재하는 240kb 크기으 octopine형 Ti 플라스미드인 pTiKU12와 45kb 크기의 잠재 플라스미드인 pTi12를 제거하여 무독성의 A. tumefaciens 균주를 제조하였다. Octopine형 Ti 플라스미드인 pTiKU12는 고온(37${\circ}C$)에서의 배양과 ethidium bromide가 첨가되어 있는 배지에서의 배양을 각각 실시하여 제거하였으며, 잠재 플라스미드인 pTi12는 pTi12의 복제기원이 클로닝되어 잇는 재조합 플라스믿인 pYWXP와의 비화합성을 이용하여 제거하였다. pYWXP는 ethidium bromide가 첨가되어 있는 배지에서 고온(37${\circ}C$)으로 배양하여 제거하였다. Agrobacterium tumefaciens KU12 contains pTiKU12 (240kb) of the octopine type Ti plamsid and pTi12 (45 kb) of the cryptic plasmid. To make the avirulent A. tumefaciens, the octopine type Ti plasmid, pTiKU12, was cured with elevated temperature (37${\circ}C$) and ethidium bromide (EtBr), respectively. Also the cryptic plasmid, pTi12, was cured by the introduction of recombinant plasmid, pYWXP, made by pTi12 replication origin and pUC19. pYWXP was cured by elevated temperature (37${\circ}C$) and EtBr simultaneously.
Gibberellic Acid 의 작용기작에 관한 연구 ( 3 ) . cyclic AMP 에 의한 단백질의 생합성 및 인산화 반응의 조절
심웅섭,노광수,문혜연 ( Woong Seop Sim,Kwang Soo Roh,Hye Yeon Moon ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.1
In order to investigate cAMP`s involvement in the action mechanisms of gibberellic acid, the effects of cAMP on the biosynthesis and phosphorylation of proteins in the germinating maize seeds were studied. cAMP promoted the biosynthesis and phosphorylation of proteins. In the presence of actinomycin D, the stimulation of protein biosynthesis by cAMP was continuous during the germination period, while the protein phosphorylation was increased at the 3-day point after germination by CAMP. The stimulatory effect of cAMP on the protein biosynthesis was greater than that on the prntein phosphorylation during the early stages of germination. On the other hand, this pattern is reversed during the latter period. The above mentioned results are consistent with the facilitation effects of GA₃ on the protein biosynthesis and phosphorylation. Therefore, it seems to be that cAMP is related to the action mechanisms of GA₃.
자궁샘근증의 한의학적 치료에 대한 국내 연구 동향 고찰
이성엽,이윤재,소민지,문혜연,김노현,서창용,도호정,송현섭,신재권,Lee, Sung-Yub,Lee, Yoon-Jae,So, Min-Ji,Moon, Hye-Yeon,Kim, No-Hyeon,Suh, Chang-Yong,Do, Ho-Jeong,Song, Hyun-Seop,Shin, Jae-Kwon 대한한방부인과학회 2017 大韓韓方婦人科學會誌 Vol.30 No.4
Objectives: The purpose of this study is to examines the domestic study trends on adenomyosis treated with Korean medicine. Methods: We investigated the studies on Korean medicine treatment for adenomyosis via searching 8 online databases. Results: As a result, 12 records (14 cases) were found to be analyzed according to their modes of diagnosis, symptoms, interventions, treatment period, follow up period and relevant outcomes. In diagnosis of adenomyosis, the most used method was ultrasonography. The most common complaint for patients with adenomyosis was dysmenorrhea. The most applied intervention for adenomyosis was herbal medication. Acupuncture was the second most used. Conclusions: Analyzing the domestic study trends would be helpful to establish a basic knowledge of the future study. It is anticipated that this review benefits the future in-depth study to confirm the efficacy of Korean medicine treatments for adenomyosis.
Gibberellic Acid 의 작용 기작에 관한 연구 - 2. Actinomycin D 처리시 GA3 에 의한 단백질의 생합성 및 인산화반응의 (燐酸化反應) 조절
심웅섭(Woong Seop Sim),박홍덕(Hong Duok Park),노광수(Kwang Soo Roh),문혜연(Hye Yeon Moon) 한국식물학회 1982 Journal of Plant Biology Vol.25 No.1
As a part of the studies on the regulatory mechanism of gene expression by gibberellic acid, the effects fo GA_3 on the protein biosynthesis and phosphorylation in maize seedlings were investigated in the presence of actinomycin D. The activites of protein biosynthesis and phosphorylation in germination seeds treated with GA_3 were greater than these of the control at the 3-day point after germination. It is assumed that the enhancement of protein biosynthesis by GA_3 in the presence of actinomycin D is due to the effects of GA_3 on the translational processes in which protein is produced from the mRNA synthesized previously.