자기면역성 뇌척수염에서 interleukin-1β converting enzyme의 발현

문창종,김승준,이용덕,신태균,Moon, Chang-jong,Kim, Seung-joon,Lee, Yong-duk,Shin, Tae-kyun 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.3

To elucidate the involvement of interleukin-$1{\beta}$ converting enzyme (ICE) in the course of experimental autoimmune encephalomyelitis (EAE), we induced EAE by immunizing rats with an emulsion of rat spinal cord homogenate with complete Freund's adjuvant supplemented with Mycobacterium tuberculosis (H37Ra, 5mg/ml) and then examined the expression of ICE in the spinal cord of rats with EAE. In normal rat spinal cords, ICE is constitutively, but weakly, expressed in ependymal cells, neurons, and some neuroglial cells. In EAE, many inflammatory cells are positive for ICE, and the majority of ICE+ cells were identified as ED1+ macrophages. During this stage of EAE, the number of ICE+ cells in brain cells, including neurons and astrocytes, increased and these cells also had increased ICE immunoreactivity. These findings suggest that the upregulation of ICE in both brain cells and invading hematogenous cells is stimulated by a secretory product from inflammatory cells, and that this enzyme is involved in the pathogenesis of EAE via the production of IL-1 beta.

자기면역성 뇌척수염 조직에서 CPP32의 면역조직화학적 관찰

신태균,문창종,안미정,위명복,Shin, Tae-kyun,Moon, Chang-jong,Ahn, Mee-jung,Wie, Myung-bok 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.3

The aim of this study was to evaluate the involvement of CPP32 (caspase-3), one of the death-related enzymes, in the course of experimental autoimmune encephalomyelitis (EAE). EAE was induced in Lewis rats immunized with an emulsion of rat spinal cord homogenate with complete Freunds adjuvant supplemented with Mycobacterium tuberculosis (H37Ra, 5mg/ml). The expression of CPP32 in the spinal cords of rats with EAE was studied. In normal rat spinal cords, CPP32 is constitutively, but weakly, expressed in neurons and some neuroglial cells. In the EAE spinal cords, many inflammatory cells were positive for CPP 32, and the majority of CPP32(+) cells were identified as ED1(+) macrophages. During this stage of EAE, the number of CPP32(+) cells in brain cells, including neurons and astrocytes, increased, and these cells also had increased CPP32 immunoreactivity. CPP32 immunor eactivity was not always matched with apoptosis of inflammatory cells in EAE lesions. We speculate that CPP32, which is constitutlvely expressed in brain cells, increases in response to neuroimmunological stimulation in both brain neuronal cells and inflammatory cells. The functional role of CPP32 in neuroimmunological disorders is discussed.

Trinitrobenzene sulfonic acid에 의해 유발된 랫드의 대장염에서 HemoHIM의 항염증 효과

이해준,김세라,문창종,김종춘,배춘식,강성수,정우희,박혜란,조성기,김성호,Lee, Hae-June,Kim, Se-Ra,Moon, Chang-Jong,Kim, Jong-Choon,Bae, Chun-Sik,Kang, Seong-Soo,Jung, U-Hee,Park, Hae-Ran,Jo, Sung-Kee,Kim, Sung-Ho 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.1

The cause and pathogenesis of inflammatory bowel disease remain unknown and no definitetherapy exists until now. The present study was conducted to investigate the anti-inflammatory effectsof a herbal preparation (HemoHIM) in colitis induced by 30 mg of trinitrobenzene sulfonic acid (TNBS)in rats. Sprague-Dawley rats were divided into 5 groups. Each group was treated with 1 mg ofHemoHIM/ml of drinking water, 4 mg of HemoHIM/ml of drinking water, 50 mg of HemoHIM/kgof body weight (i.p. once every other day) or 10 mg/kg of HemoHIMof body weight (i.p. onceevery other day) from the next day. After 2 weeks, rats were sacrificed and morphologic featuresof colons were examined. Ulceration, adhesion, thickening and dilatation were noticed in the colonicmucosa after TNBS instillation. Intraperitoneal injection of HemoHIM (50 and 100 mg/kg of bodyweight) showed the anti-inflammatory effect on adhesion, thickening, dilatation, ulceration, and theinhibition effect on damage score by 72.7% and 90.9%, respectively. Histologically, the colon of TNBS-treated rat showed inflammatory cell infiltration by polymorphonuclear cells, multiple erosive lesionsignificant improvement in these symptoms. The results obtained suggest marked anti-inflamatoryactivity of the HemoHIM at the dose levels examined.

자연방사선 고준위 지역 사육 소의 림프구 미소핵 발생 평가

이해준(Hae-June Lee),강창모(Chang-Mo Kang),김세라(Se-Ra Kim),문창종(Chang-Jong Moon),김종춘(Jong-Choon Kim),김일화(Ill-Hwa Kim),조성기(Sung-Kee Jo),장종식(Jong-Sik Jang),김성호(Sung-Ho Kim) 한국독성학회 2006 Toxicological Research Vol.22 No.4

Cytogenetic and hematological analysis was performed in peripheral blood obtained from cattle bred in the high background radiation areas (HBRA, Goesan-gun, Cheongwon-gun,Boeun-gun) and a control area. The frequencies of gamma-ray induced micronuclei (MN) in the cytokinesis- blocked (CB) lymphocytes at several doses were measured in 3 cattle. An estimated dose of radiation was calculated by a best fitting linear-quadratic model based on the radiation-induced MN formation from the bovine lymphocytes exposed in vitro to radiation over the range from 0 mGy to 1,969 mGy. The measurements performed after irradiation showed dose-related increases in the MN frequency in each donors. The results were analyzed using a linear-quadratic model with a line of best fit of y = (0.0583 ± 0.0137)D + (0.0366 ± 0.0081)D² + (0.0093 ± 0.0015) (y = number of MN/CB cells and D = irradiation dose in Gy). MN rates per 1,000 CB lymphocytes of cattle from the Goesangun, Cheongwon-gun, Boeun-gun and the control area were 6.50 ± 2.72, 9.00 ± 4.50, 10.89 ± 4.23 and 9.60 ± 4.70, respectively. The MN frequencies of CB lymphocytes from cattle bred in 4 areas mean that the values are within the background variation in this experiment. The MN frequencies and hematological values were similar regardless of whether the cattle were bred in the HBRA or the control area.

홍삼사포닌 투여의 다낭성난소에 의한 불임 치료효과 및 기작연구

김세은,오동민,심경미,정문진,임성철,나승열,이윤렬,강성수,문창종,김종춘,김성호,배춘식,Kim, Se-Eun,Oh, Dong-Min,Sim, Kyung-Mi,Jeong, Moon-Jin,Lim, Sung-Chul,Nah, Seung-Yeol,Lee, Yun-Lyul,Kang, Seong-Soo,Moon, Chang-Jong,Kim, Jong-Choon,Kim 한국현미경학회 2009 Applied microscopy Vol.39 No.1

사람에서의 PCO와 몇몇 측면에서 유사한, 실험적으로 유발된 다낭성난소(polycystic ovary, PCO)는 장기간 작용하는 estradiol valerate (EV)에 의해 유발된다. 랫드에서 스테로이드로 유발한 PCO 모델에서 홍삼 총사포닌의 역할에 대한 우리의 이전연구는 전침이 난소의 nerve growth factor (NGF) 농도를 조절하는 것을 검증하였다. 실제로 PCO와 관련된 난소 기능부전의 병리기전에서 신경성 요소의 관련성은 난소로의 교감신경유출(sympathetic outflow)의 증가로 인해 높아진다. 따라서 본 연구에서는 치료제로서의 GTS 투여가 PCO를 유발한 랫드에서 교감신경 활성을 조절할 것이라는 가설을 시험해보고자 하였다. 본 연구에서는 스테로이드로 유발한 PCO에 내재하고 있는 병태생리학적 과정과 연관된 NGF 단백질과 NGF mRNA를 분석함으로써 이루어졌다. PCO가 유발된 랫드에서 EV 대조군은 oil 대조군과 비교하였을 때 유의적으로 높은 NGF mRNA의 발현을 나타내었으며, GTS 투여군은 EV 대조군에 비해 유의적으로 낮은 NGF mRNA의 발현을 나타내었다. 그러나 NGF 단백질은 oil 대조군에 비해 EV 대조군과 GTS 투여군에서도 유의적인 변화를 발견할 수 없었다. 이러한 결과는 EV가 설치류에서 낭종 형성과 무배란을 유발하는 병리학적 단계의 구성요소일 수 있는 난소의 신경향성 단계(neurotrophic state)를 조절한다고 생각할 수 있다. Experimental induction of polycystic ovary (PCO) resembling some aspects of human PCO syndrome was produced using the long-acting compound estradiol valerate (EV). Our previous study on the role of Korean red ginseng total saponins (GTS) in a steroid-induced PCO rat model demonstrated that electro-acupuncture modulates nerve growth factor (NGF) concentration in the ovaries. In fact, the involvement of a neurogenic component in the pathology of PCO-related ovarian dysfunction is preceded by an increase in sympathetic outflow to the ovaries. In the present study, we tested the hypothesis that therapeutic GTS administration modulates sympathetic nerve activity in rats with PCO. This was done by analyzing NGF protein and NGF mRNA expression involved in the pathophysiological process underlying steroid-induced PCO. EV injection resulted in significantly higher ovarian NGF mRNA expression in PCO rats compared to control rats, and PCO ovaries were counteracted by GTS administration with significantly lower expression of NGF mRNA compared to EV treated ovaries. However, NGF protein was unaffected in both EV and GTS treated ovaries compared to control rats. These results indicate that EV modulates the neurotrophic state of the ovaries, which may be a component of the pathological process by which EV induces cyst formation and anovulation in rodents.

손바닥선인장 발효물의 급여가 이유자돈의 성장에 미치는 영향

박달수 ( Dal Soo Park ),이기현 ( Ki Hyun Lee ),문창종 ( Chang Jong Moon ),손원근 ( Won Geun Son ),신태균 ( Tae Kyun Shin ) 한국수의공중보건학회 2004 예방수의학회지 Vol.28 No.1

암컷 랫드에서 Methylcyclohexane의 단회 경구투여 독성시험

김성환 ( Sung Hwan Kim ),임정현 ( Jeong Hyeon Lim ),신인식 ( In Sik Shin ),문창종 ( Chang Jong Moon ),김성호 ( Sung Ho Kim ),신동호 ( Dong Ho Shin ),김종춘 ( Jong Choon Kim ) 한국산업위생학회 2011 한국산업보건학회지 Vol.21 No.1

The present study was carried out to investigate the potential acute toxicity of methylcyclohexane (MCH) by a single oral dose in female rats. The test chemical was administered once by gavage to female rats at dose levels 0, 1,250, 2,500, and 5,000 mg/kg. Mortalities, clinical findings, and body weight changes were monitored for the 14-day period following the administration. At the end of 14-day observation period, all animals were sacrificed and complete gross postmortem and histopathological examinations were performed. Treatment-related clinical signs, as evidenced by depression, soft feces, decreased locomotion activity, solid perineal region, crouching position, and anorexia were observed in all treatment groups in a dose-dependent manner. At the dose level of 5,000 mg/kg, decreased or suppressed body weight gain was found during the study period. At the scheduled necropsy, one case of congestion of the intestine and an increase in the weights of liver and kidney were observed in the 5,000 mg/kg group. Histopathological examinations exhibited an increased incidence of glomerular atrophy, congestion/hemorrhage, and focal degeneration/necrosis in the liver and an increased incidence of congestion, and inflammatory cell infiltration in the kidney. On the basis of the results, it was concluded that a single oral administration of MCH resulted in some adverse effects on clinical sign, body weight gain, and organ weight and histopathology in the liver and kidney in female rats. In the experimental conditions, the minimal lethal dose (LD10) of MCH was greater than 5,000 mg/kg.

랫드에서 PMMA와 Cross-Linked Dextran 혼합물의 생체 안전성 및 부피 효과에 대한 연구

장민우 ( Min Woo Jang ),진부갑 ( Bu Kab Jin ),이상훈 ( Sang Hun Lee ),박재홍 ( Jae Hong Park ),류정민 ( Jung Min Ryu ),윤승필 ( Seung Pil Yun ),박성원 ( Sung Won Park ),김형석 ( Heyong Seok Kim ),문창종 ( Chang Jong Moon ),서국현 한국조직공학·재생의학회 2010 조직공학과 재생의학 Vol.7 No.1

This study examined bio-safety and volume-related effect of the mixture of polymethylmethacrylate (PMMA) and Cross-linked Dextran as a soft tissue augmentation. To examine bio-safety and volume-related effect of the mixture of PMMA and Cross-linked Dextran in rat, gross, hematological, and pathohistological examinations were performed in comparison with CRM (R) DX (Cross-linked hyaluronic acid sodium salt, Dextranomere; commercial product) and saline. During the experimental period, there was no dead rats and scratching behavior which is a most common symptom when rejecting foreign body was observed. The volume of injected mixture was maintained sufficiently. Complete blood count(CBC) finding indicated that there were no significant differences in the number of inflammatory cells between control and experimental group. In addition, the parameters of hepatic toxicity (aspartate aminotransferase: AST, alanine transaminase: ALT, alkaline phosphatase: ALP, γ-glutamyl transpeptidase: γ- GTP, lactate dehydrogenase: LDH), renal toxicity (creatinine, blood urea nitrogen: BUN, cholesterol, albumin: ALB, phosphorus), and other organ toxicity (calcium, triglyceride, and creatine phosphokinase: CPK) using serum biochemical analysis did not show the significant differences between the control and experimental group, suggesting that systemic toxicity was not found in liver, kidney, and other organs. Histopathological findings indicated that the subcutaneous tissue had red color microsphere which capsulated by collagen near the injection site. Furthermore, the infiltration of inflammatory cells were not observed in the injected sites of all experimental groups. In conclusion, the mixture of PMMA and Cross-linked Dextran can be a safe substance for soft tissue augmentation maintaining tissue volume.

Increased expression of P53 and Bax in the spinal cords of rats with experimental autoimmune encephalomylitis

문창종,김승준,위명복,김희석,정종태,박전홍,지영흔,Tanumab, Naoyuki,Matsumoto, Yoh,신태균 濟州大學校 農科大學 動物科學硏究所 2000 動物科學論叢 Vol.15 No.1

The expression of pro-apoptotic molecules, p53 and Bax, in the spinal cord of rats with experimental autoimmune encephalomyelitis (EAE) was examined. Apoptosis was confirmed by the terminal deoxynucleotidyl transferasemediated dUTP nick end-labeling (TUNEL) method. TUSEL (+) apoptotic cells were mainly either ED1 (+) macrophages or T-cells in the parenchyma of E N . Western blot analysis showed that both p53 and Bax expression significantly ( p < 0.01) increased in the spinal cords of EAE rats at the peak stage, and thereafter declined. An immunohistochemical study showed that inflammatory cells (notably T cells) in the parenchyma express p53 and Bax, while brain cells, includng neurons and glia, were devoid of these nuclear staining of these molecules. The nuclear expression of p53 largely matches apoptotic cells in the parenchyma of EAE. These finchngs suggest that pro-apoptotic molecules, p53 and Bax, may play an important role in eliminating T cells in the parenchyma in EAE.