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?티스속근경과 탈지된 크로톤종자의 혼합추출물(CP-2)이 동물체내에서의 수종 암세포증식에 미치는 영향
문정조,김춘원,한영복,경홍기,홍은경,조영호,김종배 中央醫學社 1993 中央醫學 Vol.58 No.5
It has been reported that CP-2 extracted from the mixture of coptis and croton tiglium L. showed a cytotoxicity against various tumor cell lines. Based on this result, we have investigated furthre the effect of CP-2 on the growth of tumor cells in animal and in vivo characterization of combination antitumor chemotherapy with CP-2 and commercial chemotherapeutic agents. The most effective amount of CP-2 was 125 mg/kg in mice bearing P388 and L1210 scitic tumors, and S-180 and Ehrlich solid tumors. When the inhibitory effect on the growth of tumors were measured by tumor weight and size after inoculation with S-180 and Ehrlich cell lines, and administration of CP-2, it showed significant inhibitory effects against both cell lines. It inhibited also the growth of tumors in CDF1 mice inoculated with P388 and L1210 lines while administrations of croton tiglium L. extract along, coptis extract alone and only mitomycin-C did not show significant effects. It is interested that CP-2 enhanced the antitumor action of chemotherapeutic agents such as Picibanil, mitomycin-C and (3-interferon, suggesting that it contains a compound showing synergistic effect in addition to the compound with direct cytotoxicity to tumors, and the possibility that CP-2 can be used for a combination antitumor chemotherapy with other known chemotherapeutic drugs.
솔잎, Pinus densiflora Sieb.et Zucc., 의 항암효과(抗癌效果)에 대한 연구(硏究)
문정조,한영복,김진석,Mooon, Jeong-jo,Han, Young-bok,Kim, Jin-suk 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.4
The pine needles, Pinus densiflow Sieb. et Zucc., which is a feed for goats showing a low incidence rate of cancer were evaluated to confirm the potent anticancer effects, with or without several conventional anticancer drugs. The pine needles collected from Mt. Buk-Han located near Seoul were extracted with 95% methanol and methand and concentrated. From the methanol extract, SOM-A, was extracted dichlormethane and SOM-B was extracted with ethyl acetate. SOM-C was extracted with distilled water. These extracts were tested for their antitumor activities in vitro and in vivo. Among them, SOM-A and SOM-C exhibited potent antitumor activities described as belows. 1. The cytotoxic effects of SOM-A and SOM-C were examined against in vitro cultured murine and humman tumor cells. SOM-A showed strong cytotoxicity against human tumor cell lines and SOM-C showed strong cytotoxicity against murine tumor cell lines tested. 2. The antitumor effects of SOM-A and SOM-C were examined against P388 and L1210 of mouse ascitic tumors. The highest mean survival time(MST) ration was 151%(P388) for SOM-C(90mg/kg). 3. To compare the antitumor effects of SOM-A, SOM-B, and SOM-C against solid tumors, S-180 and Ehrlich carcinoma were implanted subcutaneously to mice on Day O. The drugs were given intraperitoneally to mice once a day on Days 1-20, and the tumor weights were measured on Day 21. SOM-A showed inhibition of tumor growth more than 50% in the experiment on S-180 and Ehrlich, and SOM-C also markedly inhibited tumor growth. However, SOM-B had no effect. 4. SOM-C combined with ${\alpha}$-interferon and SOM-C combined with Mitomycin-C enhanced the antitumor activities against murine ascitic tumors P388 leukemia.
Isoguanosine과 Berberine 1 : 1 혼합물의 항암효과
김정한,이상준,한영복,문정조,김종배,Kim, Jung-Han,Lee, Sang-Jun,Han, Young-Bok,Moon, Jung-Jo,Kim, Jong-Bae 대한약학회 1994 약학회지 Vol.38 No.2
Isoguanosine and berberine 1:1 mixture[l:[1(mole:mole)] has been prepared and evaluated by measuring antitumor effects against various tumor cell lines in culture and in mice. We reported that the synergistic effect of isoguanosine and berberine mixture has been revealed compared with each of isoguanosine and berberine increased by $3{\sim}8$ times than that of each components in various tumor cell lines in vitro. The most effective dose of isoguanosine and berberine mixture was 60 mg/kg/day in mice bearing S-180 solid tumor, the %(1-T/C) values were 70%. Against the P-388 leukemia, isoguanosine-berberine mixture was the most effective at the dose of 60 mg/kg/day, the %T/C values were 163%.
?티스속 근경과 탈지된 크로톤 종자의 혼합 추출물(CP-2)이 수종 암세포에 대한 세포독성의 효과
김춘원,서경원,조영호,홍은경,한영복,문정조,경홍기,김정한,김종배 中央醫學社 1993 中央醫學 Vol.58 No.3
In order to develop anti-cancer drug with strong anti-cancer activity without side effects, we extracted by various steps from the mixture of Coptis chinensis (CO) and Croton tiglium L (CR), and evaluated it by measuring the cytotoxicity against various tumor cell-lines including normal spleen and thymus cells. Five murine cell lines including leukemia L-1210 and 9 human tumor cell lines including leukemia HL-60 derived from various organs such as stomach, liver, lung, colon and breast cancers were used. The cytotoxicity were measured by the well-known colorimetric [3-(4,5dimethylthiazol-2-yl)2,5 -diphenyltetrazolium bromice] (MTT) assay. The extract (CP-2) from the mixture of CO and CR were very effective to all tumor cell lines with different degrees but showed no significant effects on normal spleen and thymus cells. Specially stomach SNU-1, lymphoma Raji and colon SNU-C2A compared with others showed very strong cytotoxicity; 60? 80 %, 55 ?96 % and 40~80 %, within the range of 10 ~45 ?g/ ml, 10 ~90 ?g/ml and 55 ~96 ?g/ml of the extract (CP-2) respectively. The range of ID50 values were from 4.4 ?g/ml in lymphoma Raji to 350 ?g/mi in liver HEP-3B, which were relatively low compared with other natural crude extracts reported elsewhere.