Media Card 환경에 적합한 DRM 표준 프로토콜 개발 및 기능검증

문정식 건국대학교 대학원 2006 국내석사

國際海洋法上 島山制度에 관한 硏究

문정식 경희대학교 대학원 1984 국내석사

황산리튬으로부터 침전 및 습식 전환법을 통한 수산화리튬 제조 연구

문정식 국립부경대학교 대학원 2024 국내석사

As the demand for High-Ni cathode materials increases rapidly, the importance of lithium hydroxide is emerging. Accordingly, it is necessary to study the efficient production method of lithium hydroxide monohydrate, which is an essential precursor for the production of lithium ion battery cathode materials. The purpose of this study is to explore the method of producing lithium hydroxide monohydrate from lithium sulfate monohydrate. First, lithium sulfate monohydrate was pyrolyzed and separated into lithium sulfate and water. After that, various precipitating agents (KOH, Ca(OH)2, Sr(OH)2, Ba(OH)2)) were used to compare and analyze the lithium hydroxide conversion rate of the lithium sulfate solution. As a result of the experiment, when Ba(OH)2 was used, the conversion rate was the highest at 95%. In consideration of the high conversion rate of Ba(OH)2, the optimization experiment was conducted with the lithium sulfate concentration, the reaction temperature, and the reaction ratio with the precipitating agent as variables, and a filtration process was performed to remove the precipitate. Finally, the conversion rate and lithium recovery rate of the lithium hydroxide solution were evaluated. The finally produced lithium hydroxide solution was dried at a temperature of 90°C or higher for 24 hours in a vacuum-Ar atmosphere to obtain 99.8% high-purity lithium hydroxide monohydrate.

Trichloroacetic acid 포집 방법에 의한 세포내 Peroxiredoxin의 산화환원상태 분석

문정식 전남대학교 대학원 2012 국내석사

Peroxiredoxin(Prx)은 활성 시스테인 잔기(peroxidatic Cys, CP)를 이용하는 과산화효소(peroxidase)이다. Prx는 H2O2와 반응하면 활성 시스테인 잔기(CP-SH)가 CP-SOH(sulfenic acid)로 산화되며, 다른 보존된 시스테인 잔기(resolving Cys, CR)와 각각 이황화 결합을 이루게 된다. Prx가 H2O2와 반응할 때 이차반응속도 상수는 105-107 M-1s-1이며, 전체 촉매주기의 대사 회전수(kcat)는 2-7s-1로 알려져 있다. 시험관에서 측정된 느린 대사 회전수를 토대로, Prx가 산화된 이황화 결합을 이루는 과정을 확인할 수 있을 것으로 생각되어왔다. Prx의 세포내 산화환원상태를 immunoblot으로 측정하기 위해서 Prx의 환원된 시스테인 잔기(SH)를 N-ethylmaleimide(NEM)를 이용해 알킬화하는 기존 방법 대신에, trichloroacetic acid(TCA) 침전법을 사용하여 시료를 제조하였다. 그 결과 시료 제조 방법에 따라 Prx의 이황화 결합 비율이 차이를 보였으며, 특히 Prx I보다 Prx II에서 큰 차이를 확인할 수 있었다. Prx II의 경우 여러 세포주에서 시료 제조법에 따라 약 50%의 차이를 확인하였다. 실험 결과 NEM을 처리한 기존의 방법에서는 Prx II의 이황화 결합 상태 비율이 28~63%까지 높게 나타났으나, TCA 침전을 사용하여 Prx를 빠르게 포집한 경우에는 Prx II의 이황화 결합이 약 10% 이하로 매우 낮았다. 세포주에 H2O2 처리를 한 경우에도 대부분의 Prx는 환원된 형태로 존재하였다. TCA 침전법을 사용할 경우, 원하지 않는 산화 반응이 일어나는 것을 기존의 방법보다 대폭 줄일 수 있는 것을 확인했다. 이 시료 제조 방법을 적용해 시험관에서 Prx의 산화환원 상태와, 세포내 Prx의 산화환원 상태를 정확하게 확인할 수 있었다. 앞으로의 연구에서 이 시료 제조 방법을 적용하여 Prx의 과산화효소 촉매반응 메커니즘에 대해 아직 발견하지 못한 연결 고리에 접근할 수 있을 것으로 추측한다.

政策執行에 영향을 미치는 要素에 關한 硏究 : 政策類型을 中心으로

문정식 慶熙大學校 行政大學院 1989 국내석사

國際海洋法上 海洋科學調査 制度에 관한 硏究

문정식 경희대학교 대학원 1988 국내박사

Positively expansive homeomorphisms on σ-compact metric spaces

문정식 慶尙大學校 1988 국내석사

國際海洋法上 島嶼制度에 관한 연구

문정식 경희대학교 대학원 1985 국내석사

존 칼빈과 윌리암 퍼킨스의 언약사상 : 그 연속과 발전

문정식 亞細亞聯合神學大學校 2014 국내박사

The main question I intended to address in my research for Ph.D degree is the continuity and progress of Covenant Theology between John Calvin the Reformer in Geneva and William Perkins the Puritan in Cambridge that I named “Calvin-Perkins’ chain”. In order that my dissertation makes clear their coherence by the same understanding of the covenantal interpretation of the Scripture, particularly the Decalogue i.e. the Law as the doctrinal congruence, and I present Calvin’s influence in Perkins as well as the origin of Perkins’ covenant theology through their continuity of the Christological and mutual covenant. Through this research, even though Perkins’ comprehension of the Decalogue is based on the exposition of Scripture as well as on the Reformed traditions clearly along with Augustine. As a result, it is obvious that Perkins’ covenant theology is a legacy of the influence of Augustine and Calvin, who understands the Law as the covenant with God. For this research, I would like to use the covenant of works concerning God’s grace in the Decalogue on Perkins’ base. Following Calvin’s division, if we are able to separate them, we would not find out their difference neither Sanctification in Calvin, nor the covenant activism in Perkins. In addition there is the improved one in Ames along with Calvin and Perkins, particularly the ecclesiastical application of covenant theology. Thesis : In Calvin and Perkins, there is the continuity and progress of Christological and mutual Covenant from Augustune to Ames by “Calvin-Perkins’ chain”. Tentative chapter summaries: in Chapter 1, as introduction, it is necessary to suggest four points, first of all the statement of thesis, then survey of scholarship, then the methods of this research for the expected contribution, and lastly debates on this issue. Particularly, I criticise that the opinion is R.T Kendall’s argument against Calvin and Calvinist, who insists a negative position between them in order to disconnect the continuity of the Reformers and the Puritans, especially the theological relationship between Calvin and Perkins. Thus in this dissertation, I suggest a coinage, “Calvin-Perkins’ chain” which means their continuity of Christological and mutual covenant. In chapter 2, we research what origin of Calvin’s covenant is Augustine’s. Even though the real origin Calvin’s covenant is the Bible, there is the church tradition of covenant theology from Augustine in him. This chapter shows an inchoate Christological and mutual covenant in Augustine’s De Civitate Dei , so that it reveals “Augustine- Calvin chain” as the covenantal coherence. The chapter 3 is one pole of the thesis “Calvin-Perkins’ chain”, which displays the covenant theology of Calvin. In this part we are delighted to survey Calvin’s understanding of the Decalogue in Calvin’s Commenataries and Christianae Religionis Institutio . Furthermore, he was granted Christ as the end of the Law, so that it was possible for him to be justified by faith because the law led them to Christ and the Law is the new rule to work out our salvation in Calvin’s 3rd use of the law. Consequently, we find out the Law is both the covenant of grace to lead us Christ and the new path to bring to keep itself for Sanctification. The chapter 4 is another important pole of the thesis “Calvin-Perkins’ chain”, which reveals the covenant theology of Perkins in his Armilla Aurea and other works. In works, we search out the same covenant type with Calvin, which is Christological and mutual covenant on Perkin’s interpretation of the Decalogue. Additionally, it is great to analysis Perkins’ through his same understading about the 3rd use of the Law in order to promote the believers’ spontaneous reaction to God’s grace. The more to interpret the doctrine of Sanctification by the covenant theology for the ungenerated and the generated respectively, the better to confirm the importance of Perkins’ Christological and mutual covenant like Calvin. In chapter 5, I show what Perkins’ covenant theological legacy among his descendants, William Ames, who worked for Dorts assembly in Holland and influenced Jonathan Edwards in New England, respectively. Ames’ covenant theology is particularly the ecclesiastical emphasis upon the same base with Calvin and Perkin’s covenant. Even though Ames was an exile in Holland, by his Medulla Theologiae so he influenced the Synod of Dordt to keep the covenant theology also known as TULIP as its chairman’s counselor. Furthermore Ames’ works were used to have a direct effect on the Reformed theologians to be grown in New England, especially Jonathan Edwards. Here Ames’ covenant also is able to be called “Perkins-Ames’ covenant” along with “Calvin-Perkins’ chain”. In conclusion, the thesis “Calvin-Perkins’ chain” is the substantial role to liaise the Christological and mutual covenant theology between Augustine and Ames.

Studies on biomedical characteristics of neurodegenerative diseases using AD/PD patient-derived iPSC

문정식 가천대학교 메디컬캠퍼스 일반대학원 2024 국내박사

This study explores the potential of induced pluripotent stem cells (iPSCs) in understanding and treating neurodegenerative disorders like Alzheimer's Disease (AD) and Parkinson's Disease (PD). iPSCs were generated from peripheral blood mononuclear cells (PBMC) and fibroblast samples of AD patients, and PBMC samples of PD patients, utilizing Yamanaka factors and Sendai virus transfection. This research further advanced the generation of patient-mimicking neurons from iPSCs, successfully differentiating them into dopaminergic and cholinergic neurons, demonstrating their potential as in-vitro models for AD and PD. Notably, AD patient-derived iPSCs showed distinct potential in distinguishing patient-specific features and stages of neurological disorders. The study emphasized the response of these iPSCs to oxidative stress and the effects of antioxidants at the DNA, cellular, and mRNA expression levels. Using the CUPRAC method, oxidative DNA damage and the protective effects of antioxidants such as ascorbic acid (AA), Cysteine (Cys), and N-Acetylcysteine (NAC) were assessed, revealing significant protective effects against oxidative stress. Additionally, the study found an overexpression of the TXNIP gene in AD patient-derived iPSCs, linked to inflammation in AD and suggesting its importance in drug discovery and AD treatment. This research underlines the value of iPSCs in creating disease models for neurodegenerative disorders and highlights the therapeutic potential of targeting oxidative stress and related genetic pathways in AD. 본 연구는 알츠하이머 병(Alzheimer’s Disease)과 파킨슨병(Parkinson's disease)과 같은 신경퇴행성 장애를 이해하고 치료하는 데 유도 만능 줄기세포(induced pluripotent stem cell, iPSCs)의 잠재성에 대해 탐구하였다. 알츠하이머 병 환자의 말초혈액 단핵세포(PBMC)와 섬유아세포 샘플, 그리고 파킨슨 병 환자의 PBMC 샘플에서 야마나카 인자와 센다이 바이러스 transfection을 이용해 유도 만능 줄기세포를 제작하였다. 이 연구는 유도 만능 줄기세포로부터 환자 모방 뉴런을 생성하기 위해, 도파민성 및 콜린성 뉴런으로 분화시켰으며 알츠하이머 병과 파킨슨 병에 대한 in vitro 모델로서 잠재성을 확인할 수 있었다. 특히, 알츠하이머 병 환자 유래 유도 만능 줄기세포는 신경학적 장애의 환자 특유의 특성과 단계를 구별하는 뚜렷한 잠재력을 보여주었다. 본 연구에서 이 유도 만능 줄기세포의 산화 스트레스에 대한 반응과 DNA, 세포 및 mRNA 발현 수준에서 항산화제의 효과를 확인하였다. CUPRAC 방법을 사용하여 산화 DNA 손상과 아스코르브산(AA), 시스테인(Cys), N-아세틸시스테인(NAC)과 같은 항산화제의 보호 효과를 평가했으며, 산화 스트레스에 대한 중요한 보호 효과를 보여주었다. 또한, 연구는 알츠하이머 병 환자 유래 유도 만능 줄기세포에서 TXNIP(Thioredoxin Interacting Protein) 유전자의 과발현을 발견했는데, 이는 알츠하이머 병에서 염증과 관련이 있으며 약물 타겟 발견과 알츠하이머 병 치료에서 중요한 의미가 있음을 제시하였다. 이 연구는 신경퇴행성 장애 모델을 생성하기 위한 유도 만능 줄기세포로의 가치를 강조하고 알츠하이머 병에서 산화 스트레스 및 관련 유전 경로를 목표로 하는 치료법의 잠재적 가능성을 보여주었다.