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        Cyclodextrin 분해효소의 정제 및 그 특성

        문영희,김용휘,심규광 한국농화학회 1990 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.1

        Cyclodextrin hydrolase from Bacallus stearodhermophilus KFCC 21203 was purified and the properties of the purified enzyme were investigated. The enzyme was purified 15 folds with 77% recovery by ammonium sulfate fractionation, DEAE-cellulose chromatography, and Ultro AcA 34 gel filtration. The specific activity and the molecular weight of the enzyme were 1.30 units/㎎ protein and about 29,500, respectively. The maximum activity of the enzyme was shown at 55℃ and pH 5.5. However, stable temperature and pH were 40 ℃ and 5.0∼8.0, respectively. The Km value for γ-cyclodextrin was 3.78 × 10^(-3) M. The degradation activity of the enzyme was selectively high for γ-cyclodextrin, and very low for β-cyclodextrin, but not for α-cyclodextrin. The decomposed products of γ-cyclodextrin were mainly glucose and maltose, and a little mlatotriose. The activity of the enzyme was very high for amylose, potato starch, corn starch, amylopectin and maltooligomer, and relatively high for glycogen and dextrin. The decomposed products of them were mainly glucose and maltose.

      • 아동학대관련 민간단체의 현황과 역할 및 발전 방향

        문영희 한국아동보호학회 2019 아동보호연구 Vol.4 No.2

        심각한 아동학대 사건이 발생할 때마다 정부가 제시한 아동학대대책들의 주요 내용은 ‘아동학대 조기발견’이었다. 아동학대의 조기발견을 위해 정부는 법과 제도를 통해 정책적으로 뒷받침하고, 민간영역에서는 공적영역이 놓치기 쉬운 아동학대예방 및 신고에 대한 인식개선을 위한 캠페인과 교육을 활발히 진행되는 등 다양한 노력을 하고 있다. 이러한 노력이 일환으로 아동학대 피해아동의 실제 발견율은 2001년 대비 2018년에는 12배 이상으로 증가하였다. 최근, 민간기관 차원에서 굿네이버스가 전문적인 서비스를 제공하기 위해 개발한 ‘아동보호 통합지원 전문서비스’ 모형은 학대피해아동 뿐만 아니라 가족에게도 상담, 치료, 양육기술 및 교육 등과 같은 서비스를 함께 제공하는 계기를 마련하였다. 이러한 서비스를 제공하는 다양한 민간기관들과의 협력체제 강화로 인해 민간단체에 근무하는 사회복지사들은 전문적인 상담접근이 용이해지는 등, 긍정적인 평가를 이어오고 있다. 오래전부터 미국, 영국, 호주 등과 같은 선진국에서는 아동학대에 대한 대응시스템을 공적 영역과 민간영역과의 공조체제를 갖춰서 아동학대관련 업무를 확보하고 있다. 우리나라도 민간단체로써 아동학대 업무를 전담해오던 중앙아동보호전문기관이 2020년부터는 ‘포용국가 아동정책’의 일환으로 공적영역에 편입됨에 따라 차츰 아동학대 및 아동보호와 관련된 공적전달체계가 확대되어 갈 것이다. 이에 본고에서는 한국아동학대예방협회의 창립 30주년을 맞이하여 한국아동학대예방협회를 비롯하여 아동학대 관련 5개의 민간단체(월드비전, 굿네이버스, 초록우산 어린이재단, 세이브더칠드런을 중심으로 하여 아동학대 관련 민간단체의 주요 활동을 살펴보고, 향후 아동학대관련 민간단체가 나아가야할 방향에 대해 모색해 보았다. Whenever a serious incident of child abuse occurred, the main content of the child abuse measures proposed by the government was the early detection of child abuse. For the early detection of child abuse, the government is making various efforts such as supporting policies and laws through the law and system. And the private sector actively conducted campaigns and education to improve awareness of child abuse prevention and reporting. As part of these efforts, the actual discovery rate of abused child increased more than 12 times in 2018 compared to 2001. Recently, Good Neighbors’ Professional Services for Child Protection Integrated, developed by Good Neighbors to provide professional services at the private agency level, served as an opportunity to provide services such as counseling, treatment, parenting skills and education to family of abused children as well as abused children. Due to the strengthening of the cooperative system with various private organizations that provide such services, social workers working for private organizations have enjoyed positive evaluations, such as facilitating professional counseling access. For a long time, developed countries such as the United States, the United Kingdom and Australia have secured child abuse-related work by establishing a cooperative system between the public and private sectors. Since ‘Korea Association for Prevention of Child Abuse and Neglect’, which has been dedicated to child abuse as a private organization, has been incorporated into the public domain as part of the ‘Inclusive State, Child Policy’ since 2020, the public delivery system related to child abuse and child protection will be gradually expanded. In this paper, in celebration of the 30th anniversary of ‘Korea Association for Prevention of Child Abuse and Neglect’, I reviewed five major private organizations(World Vision, Good Neighbors, Child Fund, Save the Children) related to child abuse, including ‘Korea Association for Prevention of Child Abuse and Neglect’, and I explored the future development direction of these private organizations.

      • KCI등재

        호주 애버리진 서사와 기억의 정치학—샐리 모건의 나의 자리와 케이트 그렌빌의 비밀의 강을 중심으로

        문영희 21세기영어영문학회 2019 영어영문학21 Vol.32 No.2

        This study explores how Aboriginal narratives and the politics of memory in Sally Morgan’s My Place and Kate Grenville’s the Secret River dissolve ‘terra nullius’ ideology and the Australian pioneer myth. If My Place may ask the question ‘Who owns the past,’ The Secret River would probably answer ‘the white man must bear the responsibility for Australia’s colonization.’ Morgan writes an autobiographical novel as a descendant of the Stolen Generation who were taken away from their families forcibly and exploited economically and sexually. The narrator Sally is desperately in search of the family’s secret, which is related with a white station owner’s incestuous rape in the frontier age and the crucial violences of colonization. Her journey to ‘what it means to be an Aboriginal’ has become a rewriting of Aboriginal memories and white Australian history. The Secret River seemingly deals with the story of how a convict-settler William Thornhill took up a plot of land and settled down as a gentry-like settler, but it also unsettles the legitimacy of the pioneer myth that has played a powerful role in Australian history. Grenville deploys her own history of the ancestor, Solomon Wiseman, and the novel is deliberately organized from the perspective of Thornhill. Therefore, the author makes readers pay attention to the settler’s guilt and the denial of apology.

      • KCI등재

        헨리 제임스의『특사들』 — 실용주의적 인식의 전환

        문영희 21세기영어영문학회 2015 영어영문학21 Vol.29 No.1

        This paper examines the pragmatic experiences and conversion of perception in The Ambassadors. The hero, Lambert Strether, who is engaged to Mrs. Newsome, comes to Paris as an ambassador to take her son Chad Newsome home. The dilemma comes from the fact that Strether, as a man of sensibility, is burdened with Puritan dogmas. However, his moral sense does not remain inflexible and unchanged. It undergoes considerable change as he meets with new experiences in Paris. His inner experience demonstrates a great swing from a public to a private conscience, from a fixed code of conduct to a flexible code of conduct. Strether never stops re-describing experiences by judging each event through his own ‘metaphors.' In particular, the process by which he continuously reconstructs the meanings of the experiences through his moral imagination represents the epitome of the pragmatic attitude. Strether finds himself on the point of losing everything at the end of the novel, and he decides to return to Woollett “to be right.” His decision is a pragmatic conversion that overcomes society's values and finally leads him to perceptive freedom.

      • KCI등재

        개정 「아동복지법」과 아동학대

        문영희 한양법학회 2012 漢陽法學 Vol.23 No.1

        On august 4, 2011, the Ministry of Health completely revised ‘The Child Welfare Act’(enforced 08.05.2012, Law No.11002) specifically expanding guarantees and protections of the rights of children. The new law focused founding and supplementing particularly on child abuse. Meanwhile, the problem of child abuse in our society has not garnered much attention except for the problem of sexual abuse, and child abuse becomes more problematic when parents are in a economically difficult situation. Moreover, child abuse is still treated as a problem with in the family. Not worthy of much social attention, whereas these are many laws to help the underprivileged. Under these circumstances, the revision of ‘The Child Welfare Act’ is very desirable. In particular, although this law is inferior to requiring establishment of ‘The Child Abuse Prevention Act’ in separate way through several papers and books I wrote, this is very meaningful because part of the suggested contents as the improvement method for effective prevention of child abuse and protection of the abused child were reflected. However, in this revision, while treating the child abuse problem as part of the child welfare, this law still partially deals with the problem of service about the abused child with context like overall child in need of public assistance. Also, contents about prevention, detection, processing, and post-processing,of child abuse are too incomplete, so the law is of limited use in dealing with child abuse. As a result, in this paper, we will analyze current regulations about child abuse of this law, and its problems and improvement methods will be discussed by focusing on the regulation’s contents.

      • KCI등재

        논문 : 명대(明代) 학자들의 연면사(聯綿詞) 연구 소고(小考) -《병아(騈雅)》와 《통아(通雅),련어(連語)》를 중심으로

        문영희 중국어문학회 2011 中國語文學誌 Vol.35 No.-

        聯綿詞是中國詞彙系統當中一種比較特殊的詞彙, 指的是兩個字聯綴在一起無法分開來解釋的雙音節詞。自宋代張有《複古編》<聯綿字>裏最早提出"聯綿字"槪念之後, 到了明代有兩位學者編撰了與聯綿詞有關的著作。一本是朱謀위的《변雅》, 령一本是方以智的《通雅》。《변雅》在語義、訓고方面都흔有價値。每一條目用一個訓釋詞來說明幾個聯綿詞, 通過這種訓고方式, 我們可以發現許多個聯綿詞構成一個大語義場的情況。此外, 從中旣可以考察幾個聯綿詞之間的語義關系, 比如同義關系、等義關系、上下義關系等, 同時也可對有幾個義項的多義詞加以考察。《通雅·련語》在語音方面的分析흔有參考價値。方以智根據"因聲知義"的基本觀念, 對於所收錄的聯綿詞進行分析, 闡明了聯綿詞異形之間的音轉關系。同時在每一條目下例擧一個詞的異形, 從而使我們能一目了然地看到聯綿詞的異形態。總而言之, 《변雅》和《通雅·련語》爲聯綿詞的硏究提供了흔有價値的語言資料和硏究方法, 在聯綿詞硏究史上可以說是占據了十分重要的地位。

      • 생강나무의 消炎. 鎭痛作用에 관한 연구(Ⅱ)

        文永熙,高在鍾,金庚完 朝鮮大學校 1996 藥學硏究誌 Vol.18 No.1

        Lindera obtusiloba has been used for the treatment of fever, abdominal distention and pain in traditional medicine. The anti-inflammatory activity of the extracts from Lindera obtusiloba was investigated utilizing carrageenin-induced edema, granuloma pouch in rats. The effects of this agent on capillary vascular permeability and acetic acid-induced writhing in mice were also examined. It showed to have significant analgesic activity on the writhing syndrome in mice induced by 0.7% acetic acid at Water extract of root(600㎎/㎏). It was also inhibites the increase in carpillary vascular permeability induced by acetic acid in mice. It showed anti-inflammatory activity in 1% carrageenin-induced edema with oral administeration in rats and exhibited significant preventive effect on edema at Water extract of root(600㎎/㎏). The water extract of root from Lindera obtusiloba showed significant inhibition on granuloma and exudate formation in rats. In the present study, the Water extract of root was indicated to have the analgesic and anti-inflammatory activity.

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