Detection of Human Papillomavirus In Tissues of Cervical Lesion: Comparative Study Between In Situ Hybridization and Polymerase Chain Reaction In Situ Hybridization

문성은 ( Sung Eun Moon ),( Young Tae Kim ),( Jae Wook Kim ) 대한산부인과학회 1998 대한산부인과학회 학술대회 Vol.82 No.-

현장계측 결과를 이용한 NATM터널의 지표침하영향범위 예측

채성은(Chae, Sung Eun),오한빈(Oh, Han Been),문훈기(Moon, Hoon Ki) 대한토목학회 2021 대한토목학회 학술대회 Vol.2021 No.10

근막 결손의 복원과 자궁천골인대 고정을 통한 전자궁적출술 후 질탈출의 성공적 교정

문화숙(Hwa Sook Moon),최진국(Jin Koo Choi),김경서(Kyung Seo Kim),박근식(Kyun Sik Park),황지영(Ji Young Hwang),문성은(Sung Eun Moon),한지원(Ji Won Han),김상국(Sang Gook Kim),김상갑(Sang Gap Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.7

목적 : 본 연구는 전자궁적출술 후 질탈출을 부위-특이적인 결함의 개념에 입각하여 치골경부 근막과 직장질 격막의 연결을 통한 골반내 근막의 복원과 자궁천골인대 고정술을 이용한 질탈출의 성공적인 수술적 교정의 방법과 결과를 보고하고자 한다. 연구 방법 : 1999년부터 2001년 8월까지 자궁적출술 후 질궁탈출 증상으로 문화병원 산부인과를 내원, 탈출된 질의 근막을 재건시킨 다음, 이를 자궁천골인대에 고정시킴으로써 질의 부위-특이적인 교정술을 받은 3명의 환자를 대상으로 하였다. 3명중 1명은 개복술을 하였고 2명은 내시경 수술을 하였다. 결과 : 환자의 연령 분포는 49-67세였으며, 수술시간은 평균 113분 (105-125)이었다. 세 환자 모두 수술 후 질의 상태가 해부학 및 기능적으로 정상이었으며, 9일째 특이사항 없이 퇴원하였다. 수술 후 HRT를 시행한 1명을 포함한 3명 모두 각각 24개월, 12개월, 7개월간의 추적 검사에서도 특이한 합병증이나 재발 없이 좋은 경과를 보였다. 결론 : 골반 근막 결손의 복원과 자궁천골인대 고정술은 전자궁적출술 후 질탈출의 교정에 매우 안전하고 효과적이며, 기능적인 방법으로 사료되며, 자궁천골인대를 질탈출 교정에 이용한 연구는 본 연구가 국내에서 처음이다. Objective : To report the method and efficacy of the site specific-defects repair in the endopelvic fascia and uterosacral ligament suspension for the correction of posthysterectomy vaginal vault prolapse. Methods : This study was performed in 3 patients with posthyterectomy vaginal vault prolapse who underwent the surgery for the reconstruction of endopelvic fascia and the correction of vault prolapsed vagina by using uterosacral ligament suspension at the department of Obstetrics and Gynecology in Moon-Hwa Hospital. Among them, 1 patient was treated by laparotomy, and 2 patients were treated by laparoscope. Results : The age of patients ranged from 49 to 67 years. The mean operation time was 111 minutes (105-120 minutes). The status of postoperative vagina was normal in all 3 patients, who were discharged 9 days after operation without symptoms. No complication or recurrence were found in them despite long-term follow-up for 24 months, 12 months, 7 months, respectively after operation. One patient has been treated with HRT therapy. Conclusion : The reconstruction of the defect of endopelvic fascia and uterosacral ligament fixation may be very safe, effective, and functional in the correction of vaginal vault prolapse. This study reports the treatment of vaginal vault prolapse using uterosacral ligament within Korea for the first time.

간호 대학생의 환자 프라이버시 보호행동, 간호윤리의식 및 자아존중감의 관계

성기월 ( Ki Wol Sung ),김다솜 ( Da Som Kim ),한민영 ( Min Young Han ),곽은영 ( Eun Yeong Gwak ),김근영 ( Geun Yeong Kim ),김언주 ( Eon Ju Kim ),김현지 ( Hyun Ji Kim ),류선영 ( Sun Young Ryu ),문성은 ( Seong Eun Moon ),한선미 ( Seo 대구가톨릭대학교 사회과학연구소 2011 사회과학논총 Vol.10 No.-

The purpose of this study is to understand correlation between patient privacy act, nursing ethics, and self-esteem in nursing students. Subjects of the study include 234 students in third and fourth year of four-year nursing course in universities in Daegu and the period of collecting data was between 19 and 28 September, 2011. Patient privacy act was measured by using a tool created by Lee Miyeong (2005) but revised and supplemented so that it is suitable for students nurses who are the subjects of this research. Nursing ethics was measured by the tool created by Lee Yongsook (1990) and revised by Choi Yongran (2001). Rosenberg Self-Esteem Scale (RES) developed by Rosenberg (1965) and revised by Jeon Byeongjae (1974). The collected data was analyzed by Frequency Analysis, average and standard deviation, range and t-test, one-way ANOVA, Pearson`s Correlation, Stepwise Multiple Regression. Followings are the study result. 1) The average score of patient privacy act of student nurses was over the middle mark (81.48±12.36), and the average score of nursing ethics was the middle mark (72.40 ±8.29). The average score of self-esteem was over the middle mark (31.71±3.65). 2) The significant differences of the patient privacy act were grade (p=.013) and third year students. 3) In nursing students patient privacy act was positively related to nursing ethics. 4) The significant predictors of the patient privacy act were nursing ethics, self-esteem, and grades explaining 16.9% of patient privacy act. There should be a need of educational development which can raise student nurses` nursing ethics and self-esteem in order to improve student nurses` patient privacy act based on the result of the research.

성선자극호르몬 및 GnRH agonist을 동시에 중단하는 1$\sim$2일 단기 코스팅이 임신율을 포함한 난소과자극증후군 예방에 미치는 효과

이수경,주보선,박세희,이수경,김경서,문성은,문화숙,Lee, Soo-Kyung,Joo, Bo-Sun,Park, Sea-Hee,Lee, Su-Kyung,Kim, Kyung-Seo,Moon, Sung-Eun,Moon, Hwa-Sook 대한생식의학회 2007 Clinical and Experimental Reproductive Medicine Vol.34 No.1

목 적: 성선자극호르몬과 GnRH agonist (GnRH)를 동시에 중단하는 1$\sim$2 일간의 단기 coasting이 임신율을 포함한 난소과자극증후군 예방에 미치는 효과를 조사하고자 한다. 연구방법: 체외수정시술을 위한 과배란유도 시 15 mm 이상의 난포가 20 개 이상이고 혈중 E$_2$ 농도가 4,000 pg/ml 이상일 때 coasting을 시도한 37 명의 여성들을 대상으로 하였다. Coasting은 성선자극호르몬과 GnRH agonist를 동시에 중단하였으며, 초음파상 난포의 상태와 혈중 E$_2$ 농도 에 따라 1일 또는 2일 동안 시행하였다. 혈중 E$_2$ 농도, 채취된 난자 수, 수정율, 임신율 등을 후향적으로 비교 분석하였다. 결 과: 평균 혈중 E$_2$ 농도는 coasting 시작 당일 6,993 pg/ml 에서 hCG 투여일에 3,396 pg/ml로 감소하였다. 평균 채취 난자 수와 수정율은 15.7개와 70%였다. 15명 (40.6%)이 임신을 하였고 착상율은 15.2%였다. 26명 (70.3%)이 1일, 11명 (29.7%)이 2일 동안 coasting 하였다. 평균 혈중 농도의 감소율은 1일 coasting한 군에서 43%, 2일 costing한 군은 15% (첫날)와 81% (둘째날)이었다. 임신율은 두 군간 유사하였고, 중증도 이상의 OHSS는 발생하지 않았으며 3명 (8.1%)에서 경미한 OHSS가 나타났다. 결 론: 성선자극호르몬과 GnRH agonist의 통시 중단에 의한 1$\sim$2 일의 단기 coasting은 체외수정의 결과에 영향을 주지않고 OHSS 를 예방하는데 성공적으로 적용될 수 있을 것으로 사료된다. Objective: To evaluate the effect of short coasting, by withdrawing both gonadotropins and GnRH agonist (GnRHa), on the prevention in severe ovarian hyperstimulation syndrome(OHSS) without compromising pregnancy outcome. Method: Thirty-seven women who had been coasted during COH for IVF were coasted when $\geq$20 follicles > 15 mm with serum E$_2$ level of 4,000 pg/ml were detected. Coasting was initiated for one or two days depending on the status of follicle on ultrasound and serum E$_2$ level. Both gonadotropin and GnRHa were withheld for coasting. Retrospective study was carried and changes of serum E$_2$ levels, number of oocytes retrieved, fertilization rate, pregnancy rate were compared and analyzed. Results: The mean serum E$_2$ level fell from 6,993 pg/ml on the onset of coasting to 3,396 pg/ml on the day of hCG administration. The mean number of oocytes retrieved and fertilization rate were 15.7 and 70.0%, respectively. Fifteen patients were pregnant (40.6%) and implantation rate was 15.2%. Twenty-six (70.3%) patients were coasted for one day and 11 (29.7%) were coasted for two days. The mean decrease rate of serum E$_2$ level was 43% in one day coasting group and 15% (1$^{st}$ day) and 81% (2$^{nd}$ day) in two day coasting group. The pregnancy outcome was similar between the two groups. After coasting, no severe or moderate OHSS occurred in any patients and mild OHSS occurred in 3(8.1%) patients. Conclusions: Coasting for one or two days can be used successfully in the prevention of OHSS without compromising IVF cycle outcome.

조기난소부전 여성에서 자연 임신 2예

이수경 ( Soo Kyung Lee ),주보선 ( Bo Sun Joo ),문성은 ( Sung Eun Moon ),김상갑 ( Sang Kap Kim ),구자성 ( Ja Sung Koo ),문화숙 ( Hwa Sook Moon ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.11

Premature ovarian failure (POF) is a syndrome defined as hypergonadotropic hypogonadism associated with amenorrhea, oligomenorrhea or other forms of menstrual irregularity for at least 3 consecutive months before the age of 40. The management of POF is approached by HRT, emotional support and infertility treatment. Women with premature ovarian failure who desire to become pregnant are best treated by assisted reproductive technology with donor oocyte. However, POF has the possibility of a 5-10% spontaneous pregnancy. The physician should recommend the patient to consult with their physician if they have any symptoms of pregnancy or no withdrawal bleeding after HRT. Therefore we report two cases of spontaneous pregnancies in women with premature ovarian failure.

Alendronate에 의한 MC3T3-E1 골모세포주의 분화와 Id-1과 Id-2의 발현 증가

박민정 ( Min Jung Park ),주보선 ( Bo Sun Joo ),이지은 ( Ji Eun Lee ),김현정 ( Hyun Jung Kim ),문성은 ( Sung Eun Moon ),문화숙 ( Hwa Sook Moon ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.3

목적: Alendronate는 대표적인 비스포스트네이트로서 파골세포의 활성을 떨어뜨려서 과도한 골흡수를 억제하는 약제로 알려져 왔으나, 최근에는 골세포 증식 및 분화를 증가시킨다고 보고되었다. 그러나 alendronate가 조골세포 분화에 미치는 기전에 대한 연구는 매우 부족하다. Id (Inhibitors of DNA binding/differentiation)은 helix-loop-helix (HLH) 계열에 속하는 전사인자이며, BMP에 의해 유도되는 골세포 분화과정동안 주요한 역할을 하는 것으로 알려져 있다. 그러므로 본 연구는 alendronate가 골세포 분화와 Id-1 및 Id-2 유전자의 발현에 미치는 효과를 조사하고자 한다. 연구 방법: 조골모세포인 MC3T3-E1 세포주에 alendronate를 다양한 농도 (10-9 M-10-4 M)와 시간 (24, 48 및 72시간)으로 처리한 후 조골세포로의 분화 정도를 ALP 활성도와 조골세포 분화표지자인 ALP, Col 1, OCN에 대한 발현을 역전사-중합효소반응 (RT-PCR) 방법으로 조사하였다. 또한, alendronate을 처리 후 Id-1 및 Id-2의 유전자 발현을 RT-PCR 방법으로 조사하였다. 결과: Alendronate는 ALP 활성도뿐만 아니라 ALP, Col 1 및 OCN의 mRNA 발현을 증가시키며, Id-1 및 Id-2의 발현도 증가시켰다. 이러한 alendronate에 의한 골세포 분화는 고농도보다 저농도에서 골세포 분화에 효과적이었다. 결론: 본 연구는 골형성과 관련된 전사인자 Id-1과 Id-2의 발현이 alendronate에 의한 조골세포 분화 과정동안 촉진됨을 보여주고 있으며, 이러한 alendronate 효과는 농도 의존적으로 골세포 분화에 영향을 미치는 것으로 사료된다. Objective: Alendronate, a widely used bisphosphonates, acts to inhibit bone resorption by interfering with the activity of osteoclasts. Recently, it has been reported that alendronate also may increase bone proliferation and osteoblastic differentiation. However, little is known about mechanism of the action of alendronate on osteoblast differentiation, especially in transcription level. Inhibitors of DNA binding/differentiation (Ids) are helix-loop-helix (HLH) transcription factors and play an important role in BMP-induced osteoblast lineage-specific differentiation. Therefore, this study was aimed to investigate the effect of alendronate on osteoblast differentiation and expression of Id-1 and Id-2. Methods: MC3T3-E1, pre-osteoblast cell line, were treated with alendronate of various concentrations (10-9 M-10-4 M) and time periods (24, 48 and 72 hours). And then, the effect of alendronate on osteoblast differentiation was examined by alkaline phosphatase (ALP) activity and RT-PCR for osteoblast differentiation markers such as ALP, type 1 collagen (Col 1), and osteocalcin (OCN). The expressions of Id-1 and Id-2 were measured by RT-PCR. Results: Alendronate treatment increased not only ALP activity, but also expressions of ALP, Col 1, and OCN. Also, alendronate treatment up-regulated the mRNA levels of Id-1 and Id-2 genes. This alendronate-induced osteoblastic differentiation is more effective in lower doses rather than high doses. Conclusion: This study shows that the expression of transcription factor Id-1 and Id-2 was increased in a dose-dependent manner during alendronate-induced osteoblast differentiation.

PCR in situ hybridization을 이용한 자궁경부 병변에서의 인유두종 바이러스의 검출: In situ hybridization과의 비교

김재욱(Jae Wook Kim),김영태(Young Tae Kim),문성은(Sung Eun Moon) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.11

목적: 중합효소연쇄반응법과 in situ hybridization법을 결합시킨 정위중합효소연쇄반응법 (polymerase chain reaction in situ hybridization, PCR/ISH)법을 이용하여 자궁경부병변에서 인유두종바이러스의 핵산(HPV DNA)을 규명하고, 같은 조직병변에서 in situ hybridization(ISH)법을 이용한 결과와 비교하기 위해 본 연구를 시행하였다. 연구방법 및 대상: 1998년 5월부터 7월까지 자궁경부질확대경 조준하 자궁경부생검상 자궁경부상피내종양 및 침윤성 자궁경부암으로 진단받은 15예 및 32예를 이용하여 PCR/ISH법을 적용시켜 인유두종바이러스 핵산을 규명하고, 같은 조직병변을 ISH법을 적용시켜 결과를 비교하였다. 결과: 자궁경부상피내종양에서 ISH를 사용하여 26%, PCR/ISH법으로는 80%의 양성율을 보였고, 침윤성 자궁경부암에서는 각각 40%,84%의 결과를 얻었으며, 모든 예에서 ISH양성이면 PCR/ISH양성이었고 ISH양성이면서 PCR/ISH음성인 예는 하나도 없었다. 결론: PCR/ISH법은 ISH처럼 조직학적 병변의 형태를 그대로 유지하면서, ISH보다는 훨씬 높은 검출율을 보여 효과적인 HPV DNA 검출기법이 될 것으로 사료된다. Objective: The aim of this study was to identify human papilloma virus(HPV) infection in cervical lesions by using polymerase chain reaction in situ hybridization(PCR-ISH) and to compare the results of PCR-ISH with conventional in situ hybridization(ISH) Methods: Forty-seven randomly chosen neutral-buffered formalhyde-fixed cervical biopsies in which cervical intraepithelial neoplasia and invasive cervical cancer had been diagnosed were tested for HPV DNA by PCR-ISH and ISH. The method of PCR-ISH includes deparaffinization of specimens, PCR amplification of DNA, in situ hybridization and detection of amplified products. Results: The positivity rate of ISH was 36% for all biopsies and 26%, 40% for CIN and invasive cancer respectively. By using PCR-ISH the positivity was 80%, 84% respectively. All PCR-ISH positive cases were ISH positive and no ISH positive was PCR-ISH negative. Conclusion: In our study HPV DNA, undetectable by standard in situ hybridization, was detectable by PCR in situ hybridization. Our study shows that PCR in situ hybridization is highly sensitive method for detection of HPV in cervical neoplastic specimens with morphological evidence.

혈중 E2 농도가 체외수정술의 임신율에 미치는 영향

박세희 ( Sea Hee Park ),주보선 ( Bo Sun Joo ),송수진 ( Soo Jin Song ),김경서 ( Kyung Sue Kim ),문성은 ( Sung Eun Moon ),문화숙 ( Hwa Sook Moon ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.11

목적: hCG 투여 당일 혈중 E2 농도가 체외수정술의 임신결과에 미치는 효과를 조사하고자 한다. 방법: 체외수정시술을 시행받은 455 주기를 대상으로 hCG 주사 당일 측정한 E2 농도에 따라 A군 (<1,000 pg/mL), B군 (1,000~2,000 pg/mL), C군 (2,000~3,000 pg/mL), D군 (3,000~4,000 pg/mL), E군 (≥4,000 pg/mL)으로 나눈 다음 체외수정술 결과를 비교하였다. 결과: hCG 투여 당시 혈중 E2 농도는 평균 3,745.3 pg/mL였으며, 채취된 평균 난자수는 10.1개였다. 148주기 (32.5%)에서 임상적 임신이 확인되었으며, 착상률 12.2%, 분만율 18.7%였다. E2 농도가 증가할수록 채취된 난자수는 증가된 경향을 나타냈다. 임신율 또한 E2 농도가 증가함에 따라 점차 증가하다가 D군 (50.0%)에서 가장 높은 다음, E군에서 다시 감소하였다. 38세 미만 여성에서는 전체 환자 군에서와 유사한 결과를 보인 반면, 38세 이상 여성에서는 임신율과 분만율 모두 C군에서 유의하게 가장 높았다. 결론: 이러한 결과는 혈중 E2 농도가 농도 의존적으로 임신결과에 영향을 미칠 수 있으며, 임신결과와 양성적 상관관계를 가지는 최적의 E2 농도 범위가 있음을 시사하고 있다. 과도한 E2 농도에 의한 임신결과의 저해는 여성 나이가 38세 이상일 경우 더욱 민감하였다. Objective: To evaluate the effects of serum E2 levels on the day of hCG administration on the pregnancy outcome of IVF-ET after controlled ovarian hyperstimulation (COH). Methods: Data of 455 cycles of fresh IVF-ET with COH were retrospectively investigated. Serum E2 levels on the day of hCG administration were categorized into 5 groups; A (<1,000 pg/mL), group B (1,000~2,000 pg/mL), group C (2,000~3,000 pg/mL), group D (3,000~4,000 pg/mL), and group E (≥ 4,000 pg/mL). Results: Mean E2 levels on the day of hCG administration were 3,745.3 pg/mL and mean number of retrieved oocytes were 10.1. Of 455 cycles, 148 (32.5%) cycles were clinically pregnant. Implantation rate was 12.2% and delivery rate was 18.7%. The number of obtained oocytes increased with increasing levels of serum E2. Pregnancy rate gradually increased as E2 levels increased up to the group D, but began to fall in the group E. In younger women (<38 yrs), the IVF-ET outcomes were similar to those of total patients but in older women (≥38 yrs), pregnancy rate and delivery rate were significantly higher in the group C than other groups. Conclusions: This result shows that serum E2 levels have a concentration-dependent effect on the pregnancy outcome and there is an optimal range of E2 levels to achieve for a successful pregnancy. Excessive E2 levels seem more deleterious to the pregnancy outcome in older women aged ≥38 years.

배·급수 시스템에서 부식방지제의 적용 평가 연구

우달식,문정기,구성은,최종헌,김주환,문광순 대한상하수도학회 2003 상하수도학회지 Vol.17 No.5

This study evaluated the effect of the corrosion inhibitors on the corrosion control performance in drinking water distribution system. A phosphate-zinc based inhibitor formulated was for the efficient corrosion control in drinking water distribution system. In a continuous-circulation test, 99% reduction in corrosion rate was achieved. The optimum concentration of the corrosion inhibitor was found to be 2.2㎎ PO₄/L. The effect of the corrosion inhibitor was significant for the pipes of carbon steel, galvanized steel and copper, but less for that of stainless steel. The corrosion inhibitor can be an effective cure for corrosion and red water problems by prevention the old pipes from further corrosion. Tap water in Mokpo and Kwangyang were more corrosive than the water in the rest of 4 other regions(Seoul, Pohang, Pusan, Puyeo). Tap water in Pusan was high in various ionic concentrations in general. The corrosion inhibitor reduced the corrosion rate by 76-94%, depending on the origin of the tap water.