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      • KCI등재

        Retention Factors and Resolutions of Amino Benzoic Acid Isomers with Some Ionic Liquids

        류경호,Jinzhu Zheng,Yulia Polyakova 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.6

        Ionic liquids in the form of organic salts are being widely used as new solvent media. In this paper three positional isomers, o-amino benzoic acid, m-amino benzoic acid, and p-amino benzoic acids were separated with four different ionic liquids as mobile phase additives using high performance liquid chromatography (HPLC). The following ionic liquids were used: 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]), 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIm][BF4]), 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]), and 1-octyl-3-meth-ylimidazolium methylsulfate ([OMIm][MS]). The effects of the alkyl group length on the imidazolium ring and its counterion, and the concentrations of the ionic liquids on the retention factors and resolutions of amino benzoic acid isomers were tested. The results of the separations with ionic liquids as the eluents were better than those without ionic liquids. Excellent separations of the three isomers were achieved using 2.0~8.0 mM/L [OMIm][MS] and 1.0~8.0 mM/L [EMIm][MS] as the eluent modifiers.

      • KCI등재

        Results of Urine Culture and Antimicrobial Sensitivity Tests According to the Voiding Method Over 10 Years in Patients with Spinal Cord Injury

        류경호,김윤범,양승옥,이정기,정태영 대한비뇨의학회 2011 Investigative and Clinical Urology Vol.52 No.5

        Purpose: We studied the results of urine cultures and antimicrobial sensitivity tests according to the voiding method used by spinal cord injury (SCI) patients over a recent 10-year period. Materials and Methods: We retrospectively analyzed 1,236 urine samples and their antimicrobial sensitivity tests for 112 patients who had used only one voiding method between January 2000 and December 2009. The voiding methods were classified into four groups: clean intermittent catheterization (CIC), suprapubic catheterization, urethral Foley catheter, and spontaneous voiding. Results: Of the 1,236 urine samples, 925 (74.8%) were positive and 279 (30.2%) had more than one bacteria. The CIC group showed the lowest rate of bacteriuria, colony counts, and polymicrobial infection (p<0.001). Causative organisms were mostly Gram-negative bacteria (84%), including Pseudomonas aeruginosa (22.9%), Escherichia coli (21.1%), Klebsiella species (6.7%), and Citrobacter species (6.3%). The rate of Gram-positive bacterial infection was 13.6%, and major pathogenic organisms were Streptococcus species (8.6%) and Staphylococcus species (2.6%). Major pathogenic organisms and the results of antimicrobial sensitivity tests differed according to the voiding method. Conclusions: Although the patient's condition and preferences are important when choosing the method of bladder management, CIC is the best voiding method for reducing urinary tract infections in SCI patients. When immediate use of antibiotics is needed for treatment of urinary tract infections, an appropriate antibiotic can be chosen according to the voiding method on the basis of our study and can be administered before the results of an antimicrobial sensitivity test are available.

      • KCI등재

        전문안경사를 위한 안경광학과 교육과정 개선과 개발

        류경호,김창식 대한시과학회 2013 대한시과학회지 Vol.15 No.3

        목 적: 교육과정의 개선을 모색하기 위해 교육과정의 편성을 분석하였고, 이를 바탕으로 안경사의 전문성과 미래 안목을 기를 수 있는 교육과정을 제시하고자 한다. 방 법: 교육과정의 특성을 분석하였고 전공교육과정의 확대필요성에 대해 서울시, 경기도, 전라북도에서 안경원을 경영하고 있는 안경사 112명을 대상으로 설문조사를 실시하여 분석하였다. 결 과: 안경광학과 교육과정은 교양과정이 7개 과목 15학점으로 53개 과목 109학점이 개설된 전공과정 보다 비중이 월등히 낮게 조사되었다. 전공기초 6개 과목은 17학점으로 전체 전공과목의 15.5%로 조사되었으며, 전공심화 과정은 47개 과목 92학점으로 전체 전공과목의 84.5%로 조사되었다. 안경사의 전공기초 교과목의 수업시간 확대 필요도는 기초콘택트렌즈가 6개 과목 중에서 가장 높게 조사되었으며, 전공심화 교과목은 굴절검사실습과 하드콘텍트렌즈가 12개 과목 중에 가장 높게 조사되었다. 또한 안경사의 기타의견 제시에서는 안경조제실습의 시수 확대가 38명(33.9%)으로 가장 높게 조사되었다. 결 론: 안경광학과 교육목표인 안경사 양성을 달성하기 위해 교육과정을 설계하고 이를 실천하는 과정을 교육시켜야 하며, 사회가 요구하는 시대적, 환경적 변화를 수용하는 현장중심 교육에 더 많은 시간을 수업할 수 있는 교육과정 구조 변화가 필요할 것으로 사료된다. Purpose: This study analyzed the curriculum of Ophthalmic Optics to develop curriculum organization and to suggest improved curriculum which will enhance optician professionalism. Methods: A total of 112 opticians who run optical shops in Seoul, Gyeonggi-do and Jeollabuk-do in Korea participated in the study to investigate extension of major course in Ophthalmic Optics curriculum. Results: In Ophthalmic Optics curriculum, there are 7 liberal arts subjects which are 15 credits, 53 major subjects which are 109 credits, 6 basic major courses which are 17 credits and total 14.5% of whole major courses, and 47 intensive major courses which are 92 credits and total 85.5% of whole major courses. Opticians' demand for major subjects resulted as more education time are needed on 'basic contact lens' subject in basic major courses, and 'practice in refractive' subject and 'hard contact lens' subject in intensive major courses. Also in other opinions, more 'practice in ophthalmic dispensing' subject education time is needed among major courses. Conclusion: Opticians' need in Ophthalmic Optics curriculum is to develop more practice-based major subjects to enhance real work ability.

      • KCI등재

        Theoretical Analysis of Chromatographic Peak Asymmetry and Sharpness by the Moment Method Using Two Peptides

        류경호,Du Young Choi 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.6

        The analyses of peak shapes in chromatography are useful in operating chromatographic system. The asymmetry and sharpness of a chromatographic peak are estimated by the reversed-phase adsorption of two standard peptides (angiotensin II bradykinin) on C18. In this work, the average particle diameters of C18 were 5 and 15 m, while the pore sizes were 100 and 300 . The composition of the mobile phase was 50/50 vol. % of a binary mixture of acetonitrile and water with 0.1% TFA, and the particles were packed in a stainless column (4.6 150 mm). The third and the fourth central movement were calculated from the chromatographic elution curves by moment analysis. The peak asymmetry was determined by two theoretical calculations: the asymmetry factor by elution peak analysis and skewness with moment analysis. The sharpness was estimated by the fourth central moment. In this work, the most acceptable skewness was calculated when the pore size was 300 . The large excess was observed on small pore size.

      • KCI등재

        Mobile Phase Compositions for Ceramide III by Normal Phase High Performance Liquid Chromatography

        류경호,윤현식,Seung Pyo Hong,Chong Ho Lee,김세경,Jung Heon Lee 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.1

        Ceramide III was prepared by the cultivation of Saccharomyces cerevisiae. Ceramide III was partitioned from the cell extracts by solvent extraction and analyzed by Normal Phase High Performance Liquid Chromatography (NP-HPLC) using Evaporative Light Scattering Detector (ELSD). We experimentally determined the mobile phase composition to separate ceramide III with NP-HPLC. Three binary mobile phases of n-hexane/ethanol, n-hexane/Isoprophyl Alcohol(IPA) and n-hexane/n-butanol and one ternary mobile phase of n-hexane/IPA/methanol were demonstrated. For the binary mobile phase of n-hexane/ethanol, the first mobile phase composition, 95/5 (v/v), was step-increased to 72/23 (v/v) at 3 min. In the binary mobile phase, the retention time of ceramide III was 7.87 min, while it was 4.11 min respectively in the ternary system, where the mobile phase composition of n-hexane/IPA/methanol, 85/7/8 (v/v/v), was step-increased to 75/10/15 (v/v/v) at 3 min. However, in the ternary mobile phase, the more peak area of ceramide III was observed.

      • KCI등재후보

        Parameter Estimation of Perillyl Alcohol in RP-HPLC by Moment Analysis

        류경호,Chong Ho Lee,Ji Hoon Kang 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.1

        Parameter estimations were made for the reversed-phase adsorption of perillyl alcohol (POH), a potent anti-cancer agent, on octadecylsilyl-silica gel (ODS). The average particle diameter of ODS was about 15 mm, and the particles were packed in the column (3.9 x 300 mm). The mobile phase used was a mixture of acetonitrile and water, in which the acetonitrile ranged between 50 and 70 (v/v %). The first absolute moment and the second central moment were determined from the chromatographic elution curves by moment analysis. Experiments were carried out using POH solutions within the linear adsorption range. The fluid-to-particle mass transfer coefficient was estimated using the Wilson-Geankoplis equation. The axial dispersion coefficient and the intraparticle diffusivity were determined from the slope and intercept of a plot of H vs 1/u0, respectively. The contributions of each mass-transfer step were axial dispersion, fluid-to-particle mass transfer, and intraparticle diffusion.

      • 안경광학과 전공교육과정에 대한 안경사들의 요구도 분석

        류경호 대한시과학회 2009 대한시과학회지 Vol.11 No.3

        목적: 현장에서 근무하고 있는 안경사들을 대상으로 안경광학과 전공교육과정의 영역별 주요 교과목에 대한 안경사들의 만족도, 직무적합성, 확대필요성을 분석하여 차후 교육과정 편성에 기초자료를 제공하고자 실시되었다. 방법: 서울, 경기도, 전라도 지역의 안경사 경력 5년 이하 220명을 대상으로 설문을 실시하여, 불성실 응답자 27명을 제외한 193명을 대상으로 선정하였고, 전공교육과정에 대한 영역별 교과목은 안광학, 안과학, 안경조제가공(조제가공, fitting), 검안학(굴절검사, 안기능검사, 양안시검사), 콘택트렌즈, 안경재료학의 6개 영역으로 구분하였다. 설문자료의 측정은 리커트형 5단계 척도를 이용하였으며, 수집된 자료는 t-test와 Anova 검사를 하였고, 사후검증은 Scheffe 방식으로 분석하였다. 결과: 6개 영역 이론 교과목의 만족도는 안광학을 제외하고는 비슷한 점수를 보이고 있었으며, 3개 영역의 실습 교과목(안경조제가공 영역, 검안학 영역, 콘택트렌즈)에서도 모두 비슷한 점수를 보였다. 또한 이론 교과목이 실습 교과목보다 높은 점수를 보이고 있어 이론수업을 실습수업보다 더 만족하는 것으로 나타났다. 전공교육과정에 관해 안경사들이 느끼는 직무적합성은 6개 영역 이론 교과목에서 이론만 있는 영역의 교과목보다 이론과 실습이 있는 과목의 직무적합성이 더 높은 것으로 나타났고, 3개 영역의 실습 교과목은 절대점수(3점) 보다 높은 4.45로 조사되었다. 전공교육과정의 확대필요성은 6개 영역 이론 교과목 중 안경조제가공과 검안학만이 절대점수(3점) 보다 높게 나타난 반면, 3개 영역의 실습 교과목에 대해서는 모두 4점(필요하다) 이상으로 조사되었다. 3개의 실습교과목 영역 가운데 검안학 영역의 점수가 가장 높았으며, 검안학 교과목 중에서도 굴절검사 실습 과목이 가장 높게 조사되었다. 결론: 안경사들은 전공교육과정에서 안경조제가공 영역과 검안학 영역의 이론 및 실습 과목을 확대하여 편성해야 한다고 인식하고 있었고 특히, 굴절검사의 실습 교과목의 확대필요성을 인식하고 있었다. Purpose: This research was practiced for provide a mental data an organization educational courses after analyzing optician`s satisfactory degree, relevance of duty and necessity of extension about major subjects at region of major educational courses of Ophthalmic Optics in the subjects of optician`s who working in the optical shop. Methods: By making up questionnaire of 220 person who had under 5year experience in working in Seoul, Gyeonggi-do and Jeolla-do, and selecting 193 person except 27 person who answered the questionnaires insincerely. And the major educational courses were classified into six regions: Ophthalmic Optics, Ophthalmology, Optical Dispensing & Fitting(Optical Dispensing, Optical fitting), Optometry(Refraction, Eye Examination, Binocular Vision), Contact lens and Ocular Optics Materials. We made use of the pattern Likert five-step index, and the collected data were inspected in t-test and Anova. Post verification was practiced in analyzing then in the method Scheff`e. Results: The satisfactory degree in 6 theoretical courses showed similar except Ophthalmic Optics, and the 3 practice courses(Optical Dispensing & Fitting, Optometry, Contact lens) got similar marks. Also it appeared that they were more satisfied with theoretical lessons than practical lessons in that the theoretical subjects got higher marks than the practical subjects. And the relevance of duty which optician`s felt about major educational courses was higher more in the both theoretical and practical courses than in the only theoretical courses in the 6 theoretical courses. The 3 practical courses got 4.45 higher than the absolute mark(3). While necessity of extension about major educational courses showed that the marks of Optical Dispensing & Fitting, Optometry in the 6 theoretical courses only were higher than the absolute mark(3), those of the 3 practical courses were more than 4. The mark of Optometry was the highest of those of 3 practical courses, and the Refraction is the highest in the subject of Optometry. Conclusions: Opticians recognize that the theoretical and practical subjects of Optical Dispensing & Fitting and Optometry in the major educational courses must be extension, and they especially recognized necessity of extension of practical subjects in Refraction.

      • KCI등재

        生體 氣에 대한 波動共鳴的인 연구

        류경호,김경철,이용태 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.2

        The present condition of study on the wave-resonance for the bio-KI is observed. And the probroms of reception attendant on the stimulation of wave is observed centering around the sensory organ. The results is as follows: In the wave-resonant stand point, the tendency of studying on KI is showed in the several field all over the world. Because it is originated radionics, the wave-resonant tools of MRA insistenting minute-magnetism-resonance-apparatus need the more severe data in the side of electric circuit. The wave resonance apparatus according to the frequency occurance transmits low-frequency's vibration ratio to the electric stimulating aspect. The wave-water is considered on the application of wave-resonance transcription on the water, and needs the comprehension of torsion-field level. The wave-stimulation of the bio-KI and the reception of the sensory-organ is observed the connection of the sensory-organ and it's corresponding wave. The informations recorded in the wave are distinguished patternly. And the several shaping waves transmit the informations each other through the resonnance. The wave theory is explained the LEE(理) as the wave-pattern and the KI(氣) as the revelation of the patterned-wave in the LEE-KI theory(理氣論), moreover the SHIN(神) as the information of the wave-pattern and the KI as the energy of the wave and the JEUNG(精) as the material of the wave in the JEUNG-KI-SHIN theory(精氣神論). In this point, the study on the wave-resonance of bio-KI is thought that it is significantly in the study method for oriental medicine. The sum of the wave makes the moving body and forms the universe. It means that the several wave patterns gather and form new field. The pattern is a kind of the information and the information is not materially. The information of wave-pattern is the arrangement and the combination of the material source.

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