한국재래닭의 MHC 유전자형 분석

라세둘,정기철,서동원,최강덕,이준헌 한국가금학회 2009 한국가금학회 정기총회 및 학술발표회 Vol.26 No.-

The chicken major histocompatibility comple(MHC) is known to associated to disease resistance and susceptibility to several pathogens. Within the chicken MHC between BG and BF region, the microsatellite marker LEI0258 is known to be highly related with the serological haplotypes and 51 different haplotypes were already identified. In this study, the LEI0258 genotypes were investigated for identifying MHC allele diversity in Korean native chicken. The MHC haplotype was analyzed by PCR screening and sequencing of the LEI0258 region in 4 different chicken breeds including Korean native chicken black, Korean native chicken brown, Cornish and Rhode island red. Upon verification of the identified alleles, this results will greatly contribute for breed identification markers, with combination of other valuable markers.

Identification of SNPs in Highly Variable Lysozyme Gene in Korean Native Chicken Populations

라세둘,강보석,임희경,최강덕,이준헌 충남대학교 농업과학연구소 2010 농업과학연구 Vol.37 No.3

Single nucleotide polymorphisms (SNPs) in chicken lysozyme (LYZ) gene were investigated in this study. The identification of SNPs in both exon and intron in LYZ gene has led to understanding of evolution for the domestic chicken populations. A total of 24 samples from two Korean native commercial chicken populations (CCPs) were used for the initial identification of SNPs by mixing three DNA samples for sequencing experiments. By comparing with red jungle fowl (RJF), two commercial chicken populations have 18 common polymorphisms. Between two commercial chicken populations, 15 polymorphisms were identified. Of the 33 polymorphisms identified, two indels (21 and 4 bp) were found. Whereas, only one polymorphism in exon 2 at the bp position 1426 was a non-synonymous substitution (p.Ala49Val), indicating the amino acid changes. The identified non-synonymous substitution (p.Ala49Val) is located close to the catalytic sites of the enzyme, which might affect its activity. In our investigation,the polymorphisms in LYZ gene can provide broad ideas for the variation of Korean native chicken populations from the ancestor of chicken breeds as well as the some biological functions of the LYZ gene.

Identification of SNPs in Highly Variable Lysozyme Gene in Korean Native Chicken Populations

라세둘,강보석,임희경,최강덕,이준헌,Hoque, M.R.,Kang, B.S.,Lim, H.K.,Choi, K.D.,Lee, J.H. Institute of Agricultural Science 2010 Korean Journal of Agricultural Science Vol.37 No.3

닭의 진화를 이해하기 위하여 변이가 많다고 알려진 LYZ 유전자의 엑손과 인트론에 존재하는 단일염기다형이 본 연구를 통해 확인되었다. 2개의 한국 재래실용계에서 총 24개체의 DNA 샘플이 본 연구에서 이용되었으며 단일염기 다형의 확인을 위하여 3개체의 샘플을 혼합하여 염기서열 분석을 실시하였다. 적색야계와의 비교를 통하여 두 한국 재래실용계는 18개의 염기서열변이를 확인할 수 있었으며 한국 재래실용계 간에는 15개의 염기서열 변이를 확인할 수 있었다. 총 33개의 변이 중 두 개의 삽입변이(21 bp와 4 bp)가 확인되었다. 한편, 2번째 엑손의 1426 bp 위치에 존재하는 단일염기 다형(p.Ala49Val)은 아미노산의 변이를 나타내는 미스센스 돌연변이로 확인되었다. 이 돌연변이는 이 lysozyme 효소의 촉매작용을 하는 위치에 놓여 있어 효소의 활성과 밀접한 관계가 있을 것으로 추정된다. 본 연구에서 밝혀진 LYZ 유전자의 변이는 이 유전자의 기능뿐 아니라 한국 재래실용계 집단의 구조를 이해하는데 기초자료로 이용될 것으로 사료된다. Single nucleotide polymorphisms (SNPs) in chicken lysozyme (LYZ) gene were investigated in this study. The identification of SNPs in both exon and intron in LYZ gene has led to understanding of evolution for the domestic chicken populations. A total of 24 samples from two Korean native commercial chicken populations (CCPs) were used for the initial identification of SNPs by mixing three DNA samples for sequencing experiments. By comparing with red jungle fowl (RJF), two commercial chicken populations have 18 common polymorphisms. Between two commercial chicken populations, 15 polymorphisms were identified. Of the 33 polymorphisms identified, two indels (21 and 4 bp) were found. Whereas, only one polymorphism in exon 2 at the bp position 1426 was a non-synonymous substitution (p.Ala49Val), indicating the amino acid changes. The identified non-synonymous substitution (p.Ala49Val) is located close to the catalytic sites of the enzyme, which might affect its activity. In our investigation, the polymorphisms in LYZ gene can provide broad ideas for the variation of Korean native chicken populations from the ancestor of chicken breeds as well as the some biological functions of the LYZ gene.

DNA markers in chicken for breed discrimination

라세둘,이승환,이준헌 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.2

There is an emerging interest in using DNA markers for breed identification in animals. This article reviews the breed identification markers in chicken, mainly developed in Chungnam National University, with particular emphasis on the mitochondrial DNA markers and the nuclear DNA markers including the SNPs in MHC region and the plumage color related MC1R markers. This information would be very useful for an appropriate conservation breeding program as well as for the establishment of molecular markers for chicken breed identifications.

DNA markers in chicken for breed discrimination

라세둘,이승환,이준헌,Hoque, Md. Rashedul,Lee, Seung-Hwan,Lee, Jun-Heon Institute of Agricultural Science 2012 Korean Journal of Agricultural Science Vol.39 No.2

There is an emerging interest in using DNA markers for breed identification in animals. This article reviews the breed identification markers in chicken, mainly developed in Chungnam National University, with particular emphasis on the mitochondrial DNA markers and the nuclear DNA markers including the SNPs in MHC region and the plumage color related MC1R markers. This information would be very useful for an appropriate conservation breeding program as well as for the establishment of molecular markers for chicken breed identifications.

DNA markers in chicken for breed discrimination

Md. Rashedul Hoque(라세둘),Seung-Hwan Lee(이승환),Jun-Heon Lee(이준헌) 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.2

There is an emerging interest in using DNA markers for breed identification in animals. This article reviews the breed identification markers in chicken, mainly developed in Chungnam National University, with particular emphasis on the mitochondrial DNA markers and the nuclear DNA markers including the SNPs in MHC region and the plumage color related MC1R markers. This information would be very useful for an appropriate conservation breeding program as well as for the establishment of molecular markers for chicken breed identifications.

Identification of SNPs in Highly Variable Lysozyme Gene in Korean Native Chicken Populations

M. R. Hoque(라세둘),B. S. Kang(강보석),H. K. Lim(임희경),K. D. Choi(최강덕),J. H. Lee(이준헌) 충남대학교 농업과학연구소 2010 농업과학연구 Vol.37 No.3

닭의 진화를 이해하기 위하여 변이가 많다고 알려진 LYZ 유전자의 엑손과 인트론에 존재하는 단일염기다형이 본 연구를 통해 확인되었다. 2개의 한국 재래실용계에서 총 24개체의 DNA 샘플이 본 연구에서 이용되었으며 단일염기 다형의 확인을 위하여 3개체의 샘플을 혼합하여 염기서열분석을 실시하였다. 적색야계와의 비교를 통하여 두 한국재래실용계는 18개의 염기서열변이를 확인할 수 있었으며 한국 재래실용계 간에는 15개의 염기서열 변이를 확인할 수 있었다. 총 33개의 변이 중 두 개의 삽입변이(21 bp와 4 bp)가 확인되었다. 한편, 2번째 엑손의 1426 bp 위치에 존재하는 단일염기 다형(p.Ala49Val)은 아미노산의 변이를 나타내는 미스센스 돌연변이로 확인되었다. 이 돌연변이는 이 lysozyme 효소의 촉매작용을 하는 위치에 놓여 있어 효소의 활성과 밀접한 관계가 있을 것으로 추정된다. 본 연구에서 밝혀진 LYZ 유전자의 변이는 이 유전자의 기능뿐 아니라 한국 재래실용계 집단의 구조를 이해하는데 기초자료로 이용될 것으로 사료된다. Single nucleotide polymorphisms (SNPs) in chicken lysozyme (LYZ) gene were investigated in this study. The identification of SNPs in both exon and intron in LYZ gene has led to understanding of evolution for the domestic chicken populations. A total of 24 samples from two Korean native commercial chicken populations (CCPs) were used for the initial identification of SNPs by mixing three DNA samples for sequencing experiments. By comparing with red jungle fowl (RJF), two commercial chicken populations have 18 common polymorphisms. Between two commercial chicken populations, 15 polymorphisms were identified. Of the 33 polymorphisms identified, two indels (21 and 4 bp) were found. Whereas, only one polymorphism in exon 2 at the bp position 1426 was a non-synonymous substitution (p.Ala49Val), indicating the amino acid changes. The identified non-synonymous substitution (p.Ala49Val) is located close to the catalytic sites of the enzyme, which might affect its activity. In our investigation, the polymorphisms in LYZ gene can provide broad ideas for the variation of Korean native chicken populations from the ancestor of chicken breeds as well as the some biological functions of the LYZ gene.

SNP typing in Korean Native Chicken Mitochondrial D-loop region for Breed Identification

M. R. Hoque(라세둘),B. S. Kang,H. K. Lim,K. D. Choi,J. H. Lee 한국가금학회 2010 한국가금학회 정기총회 및 학술발표회 Vol.27 No.-

The mitochondrial (mt) DNA D-loop sequences have been widely used for identification of origin of the breeds in animals. In this study, single nucleotide polymorphisms (SNP) from chicken mtDNA were investigated for identifying breed differences between the two commercial Korean native chicken (KNC) breeds. A total of 566 individual birds were used in this study. Of these, 280 mtDNA sequences were obtained from different chicken breeds. The sequence data indicated that there are 14 mtDNA clades from the 27 nucleotide variations. We also investigated whether the D-loop hypervariable region in chicken mtDNA found four specific SNPs can be used for the breed identification. In addition to our previous study, three more SNPs were also included for discrimination of KNC breeds. Using the seven SNPs markers, the probabilities of breed identification in CCP1 and CCP2 were 78.4% and 90.8%, respectively. The results obtained in this study can help for the consumers when they select the KNC meats.

mtDNA의 COI 유전자 변이를 이용한 한국 야생오리 및 집오리의 계통분류학적 관계

진선덕,라세둘,서동원,백운기,이준헌 한국가금학회 2011 한국가금학회 정기총회 및 학술발표회 Vol.28 No.-

한국 재래닭의 MHC 영역 유전자형 분석

정기철,라세둘,서동원,박병권,최강덕,이준헌,Jung, Kie-Chul,Hoque, Md. Rashedul,Seo, Dong-Won,Park, Byung-Kwon,Choi, Kang-Duk,Lee, Jun-Heon 한국가금학회 2009 韓國家禽學會誌 Vol.36 No.4

닭의 MHC 유전자 내에 있는 microsatellite marker LEI0258은 혈청의 구분 및 질병 저항성 유전자로 많은 연구가 되어 있다. 본 연구는 유전변이가 있는 microsatellite marker LEI 0258을 한국 재래계 흑색종, 한국 재래계 갈색종, 코니쉬종, 로드아일랜드 레드종에 적용하여 품종 특이 유전자형 및 allele을 탐색하고, 품종 구분 활용에 가능하지 여부를 실험하기 위하여 실시하였다. 한국 재래계만이 가지고 있는 특이 대립 유전자는 찾을 수는 없었지만 품종간 유전자형의 빈도 차이를 보이는 대립 유전자들을 확인할 수 있어 마커의 조합을 통하여 품종을 구분할 수 있는 가능성을 제시하였으며, 질병 저항성 연구의 기초 자료로 그 이용성이 높을 것으로 사료된다. The chicken major histocompatibility complex (MHC) is known to be associated with disease resistance and susceptibility to several pathogens. The microsatellite marker LEI0258 is physically located between the BG and BF of MHC region and variations near this marker have been well documented. In this report, the LEI0258 marker was used to find specific alleles for the Korean native chicken. The MHC haplotype was analyzed by PCR screening and sequencing of LEI0258 region in four different breeds including black Korean native chicken, brown Korean native chicken, Cornish and Rhode island red. The serologically same MHC haplotypes showed the differences in repeat numbers, a few indels or single nucleotide polymorphisms by sequencing analysis. Even though we could not identify specific alleles for Korean native chickens, the genotypes analyzed in these breeds can give valuable information for the relationships with disease resistance and establishment of breeding strategies for the Korean native chicken.