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홍화씨가 치은섬유모세포, 치주인대세포 및 골아세포에 미치는 효과
두진수,신형식 원광대학교 치의학연구소 1998 圓光齒醫學 Vol.8 No.1
The purpose of this study was to evaluate the effects of Carthamus tinctorius LL on gingival fibroblasts, periodontal ligament cells and osteoblasts. Periodontal ligament cells and gingival fibroblasts were primarily from extracted premolar with non-periodontal diseases. Osteoblasts were getten from calvariae of rat. Cells were cultured with DMEM at 37℃, 5% CO_2, 100% humidity incubator. MTT assay and collagen synthesis were used as a method of this paper. The obtained results were as follows : 1. The cellular activity of gingival fibroblasts was significantly increased in 10^-4g/㎖ of Carthamus tinctorius L. at the culture of the first, the second and the third day. 2. The cellular activity of periodontal ligament cells was significantly increased in 10^3g/㎖ of Carthamus tinctorius L. at the culture of the first, the second and the third day. 3. The cellular activity of osteoblasts was increased and maintained in 10^-3g/㎖ and 10^-4g/㎖ of Carthamus tinctorius L. but was significantly decreased over the level of 10^-3g/㎖. 4. The collagen synthesis of periodontal ligament cells was significantly more increased in 10^-3g/㎖ than that of the control. and the collagen synthesis of osteoblasts was significantly increased in 10^-3g/㎖ too. These findings suggest that increased cellular activity and collagen synthesis by Carthamus tinctorius L may help the periodontal regeneration and reconstruction.
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두진수,강정구,유형근,신형식,Doo, Jin-Soo,Kang, Jung-Ku,You, Hyung-Keun,Shin, Hyung-Sik 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.3
Healing of periodontal tissues require the migration and proliferation of gingival fibroblasts and periodontal ligament cells. There is many evidences that the some agents like cytokines and polypeptide growth factors are mediate these cellular events in wound healing. Recently someone is interested in herbal drugs on periodontal tissue healing processes. The purpose of this study was to examine the effects of 4 herbal drugs, Carthami Flis, Moutan Redias Cortex, Scirpi Rhisoma, Seed of Carthamus tinctorius L. on human gingival fibroblasts and periodontal ligament cells. Periodontal ligament cells and gingival fibroblasts were primarily cultured from extracted premolar with non-periodontal diseases. The powder from extracted. herbal drugs were prepared with distilled water. Cells were cultured with DMEM at $37^{\circ}C$, 5% $CO_2$, 100% humidity incubator, and treated with each herbal drugs with proper concentration for 1, 2, and 3 days. The cell activity was determined by ELISA reader using MTT assay. There was the most significant elevation in $10^{-3}g/ml$ of almost herbal drugs on cellular activities. The result of this study demonstrated that Carthami Flis, Moutan Radicis Cortex, Scirpi Rhisoma, Seed of Carthamus tinctorius L. appears to have beneficial effect on healing process after periodontal treatment.
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