土川芎과 日川芎(Cnidium officinale Makino)의 細胞分類學的 硏究

都貞愛 梨花女子大學校 韓國生活科學硏究院 1969 韓國生活科學硏究院 論叢 Vol.2 No.-

A comparative study of the gros morphology and cytology of Toh Chun Gung and Ⅲ Chum Gung, the two taxa of Cnidium officinale MAKINO growing in Korea was carried out. The taxon of Toh Chun Gung is taller that of Ⅲ Chun gung and shows greater growth rate than that of the latter. The root of the former is irregular in shape, having large branches and that of the latter is taro-like in shape and has nodules. The pollens of oth taxa are subspheroidal or ellipsoidal, but the pollens of Toh Chun gung are sometimes subtriangular. The somatic chromosome number of Toh Chun Gung was found to be 2N=33 and that of Ⅲ Chun Gung 2N=22. Toh Chun Gung is considered as a triploid which may have originated from between Ⅲ Chun Gung, a diploid, and a tetraploid of the latter or other taxon of the genus Cnidium. The pollen fertility of Toh Chun Gung was found to be very low or almost zero.

半夏와 大半夏 形態學的 比較

都貞愛 梨花女子大學校 韓國生活科學硏究院 1979 韓國生活科學硏究院 論叢 Vol.22 No.-

南海岸 島嶼地에 自生하는 大半夏는 이미 記載된 半夏와 뚜렷한 差異가 있는 點으로는 着眼 두 種의 外部形態, 內部構造, 顯微化學的 및 染色體數를 調査한 결과 다음과 같은 所見을 얻었다. 外部形態가 큰 大半夏의 小葉은 廣 型 楕圓形에 基部가 深心?型이며 葉先이 銳尖頭形인데 半夏는 狀披針形이며 三出葉 合點部位와 葉柄下端에는 珠芽가 있고 肉芽가 붙은 球莖이나 大半夏에는 珠芽나 肉芽가 없었다. 大半夏 球莖組織에 있어서 두꺼운 cork층과 組織內 周邊部에 粘液道(mucilage duct)가 發達되었고 그속에 顆粒狀物質(granular substance)이 含有하고 있었다. 이 顆粒은 여러 染色反應과 Enzyme反應에 의해서 Phospho protain polysaccharide 物質이 들어 있음이 確認되어 有物의 현저한 差異가 있었다. 染色體實험에서 半夏의 染色體數는 112數로 나타났고 大半夏에서는 52數로 나타났다. 위에 所見으로 보아 大半夏는 半夏와 別個의 種으로 생각되며 未記錄種이다. This Comparative study was performed in order to invetigate taxanomic difference between Pinellia ternata and Pinnellia tripartita, the former having been collected in Kyung-gi province, and the latter, in chung-mu province southern coast of Korea. Howerever, Pinellia tripartita is still unrecorded in Korean botanical lists. the externaal morphologic features, anatomical structures, histochemical and cytological characteristics of these species are compared and observed. The results of the study are follows : The size of every organ in Oinellia tripartita is larger than those of pinellia ternata . Pinellia ternata have bulbillus of leaf petiol and lateral bud in the tuber, though such organs are not observed in pinellia tripartita. In the observations of microscopic structures, there is granular substance in mucilageduct of Pinellia trpartita but such a granular substance was not observed in Pinellia ternata. Histochemical and enzyme tretments revealed that the granular substance is a compound which is composed of phosphate protein and polysaccharidse. The diploid number of chromosomes in the tip cell of pinellia ternata were 112, while in pinellia tripartita the number was 52. It is concluded that these two plants are nto identical with each other, and the plant of the pinellia tripartita is to be enlisted newly into Korean botanical lists.

滋襄强壯劑의 粘質性 生藥에 관한 硏究 : Liliaceae의 麥門冬, 天門冬 및 黃精類

都貞愛 梨花女子大學校 韓國生活科學硏究院 1987 韓國生活科學硏究院 論叢 Vol.39 No.-

Liliaceae에 屬하고 滋養, 强壯, 强精藥으로 흔히 쓰이며, 資源이 풍부한 粘質性의 生藥인 麥門冬, 天門冬, 黃精, 玉竹에 關하여 生藥學的으로 比較 硏究하였다. 生藥形態가 類似하고 臨床的으로 같은 용도로 쓰이는 맥문동, 천문동과 黃精 玉竹의 形態 및 內部構造를 觀察하고 生藥粉末을 檢體로 하여 理化學的 實驗과 抗菌實驗을 시도하였다. 맥문동, 천문동의 塊根 皮層애 작은 形態의 束針晶이 나타나고 황정과 옥죽 根莖組織內에서는 粘液質을 함유한 粘液道와 큰 형태의 束針晶이 많이 觀察되었다. 物性實驗에서 各 生藥粉末의 性狀은 다르게 나타났으며 一般成分分析에서 含量의 差異는 근소하였다. 抗菌실험에서 各 生藥의 水浸液凍結乾燥 粉末과 EtOH Extract를 檢液으로 하여 Bacillus subtilis, Staphylococcus aureus, pseudomonas areuginosa 및 Escherichia coli에 對한 抗菌作用의 유의성을 平板培養法으로 檢定한 結果 EtOH Ex.의 各 檢液에서 各 種菌에 對하여 濃度差異를 두고 抗菌性이 나타났으나 水浸液檢液에서는 나타나지 않았다. 結果 各 粘質性生藥間의 共通的인 特徵과 差異點이 比較되었다. Several crude drugs in Liliaceae which are frequenty employed in Chinese drug dispensing as nutritious or tonic agents were investigated to detect any differences on morphological, physico-chemical and antimicrobial properties. Crude drugs tested were Liriopis Tuber, Asparagi Tuber, Polygonati Rhi-zoma and Polygonati Rhizoma (Oc-juck). The findings of this study were as follows. 1. Small Raphides of ca-oxalate were observed in the cortex of Tuber and large Raphides in abundant mucous substance were presented in the Rhizoma. 2. The result of physico-chemical analysis showed some differences in chemical constituents. 3. The EtOH Ext. of Liriopis Tuber, Polygonati Rhizoma, Polygonati Rh-izoma(Oc-juck) showed antibacterial activities (MIC<100mg/ml). Asparagi Tuber showed antibacterial activities against Bacillus substilis(50mg/ml<MIC<100mg/ml), S. aureus. P. aeruginosa and E. coli (100mg/ml<MIC<250mg/ml). No antifungal activities were observed from both the aqueous Ext. and EtOH Ext. of these crude drugs.

Morus alba L.(桑根皮)의 形態學的 硏究

都貞愛 梨花女子大學校 韓國生活科學硏究院 1980 韓國生活科學硏究院 論叢 Vol.26 No.-

漢方에서 桑白皮는 Morus alba系와 M. bombycis系 等의 變種, 品種 또는 여러 雜種의 것을 混用하고 있다. 本 硏究의 目的은 Morus alba系에 屬하는 水原大葉, 龍用秋雨 및 개량서변의 桑根皮에 對하여 形態學 및 組織學的 見地에서 差異點을 比較하는데 있다. 1.水原大葉의 根皮 겉껍질은 淡褐色~黃褐色이며, 暗紫褐色의 코르크 껍질이 떨어지기 쉬으며, 紫色의 세로 주름을 이루고, 內側面은 엷은 黃色~엷은 褐色의 섬유성이며, 두께는 1.0~2.5mm이다. 龍川秋雨의 것은 失褐色~赤褐色의 外皮에 赤紫色의 코르크껍질이 全面 세로 주름을 많이 만들고, 內側面은 黃白色이고, 두께는 0.7~3.0mm이다. 개량서변의 것은 褐色~赤褐色의 外皮 暗紫色의 코르크껍질이 세로 주름을 잘게 이루며, 赤褐色을 띤 많은 皮目이 있다. 內側面은 淡黃褐色이며, 두께는 0.7~2.0mm이다. 2. 세 종류의 根皮의 內部組識學的構造는 대체로 類似하나 龍川秋雨의 것이 皮層 內에 乳管의 分布數가 많았으며, 수선의 방사조직이 특히 발달되었다. 3. 水原大葉 개량서변에 있어서는 대체로 皮層 內의 수선이 규칙적으로 배열되고 수산칼슘의 결정이 많이 관찰되었다. 4. 이들 3種의 根皮組織內에 分布된 篩部纖維(bast tiber)는 肥厚된 膜들이고 本質化는 거의 되지 않고 있다. 5. 根皮 皮層 內에 많이 分布된 수상칼슘결정형은 單晶~多角形이며, 이들의 결정들은 유기물질 및 무기물질과의 集合體 結晶으로 보아진다. 본 연구의 실험재료 수집에 협조하여 주신 경희대학교 약대 유경수 교수님께 감사를 드립니다. In Korea, the mixtures of dried roots of Morus alba L. and Morus bombycis, and other varieties and hybid Morus are ofren used for oriental herb drug preparation. Some confusion and problems are raised in the evaluation of drug action as the material are not homologous. The aim of this study is to establish criteria which distinguish those materials to help more precise preparing of crude drugs with Morus alba L. series. Morphological and histological experiments have been applied and then htese sseries are compared each oter. The obtained results are as follows: (1) Root bark of Morus alba L. (Soo Won Dae Yup) shows dark yellowish brown colour, and outer cork is purple in colour and shrunk around the bark. The inner layer of the cork shows light yellowish brown colour and the thickness is between 1.0mm to 2.5mm. Root bark of Morus alba L. (Yong Chun Choo Woo) is light brown in colour and outer cork shows red brown. Transverse shrunken bands are observed, its inner layer is white-yellow in colour, the thickness is 0.7mm to 3.0mm. Root bark of Morus alba L. (Kae Ryan Seo Ban) shows red-brown, and outer cork is dark-purple in colour. Long shrunken bands are laying transversely. The colour of inner layer of cork isyellosish brown, and the thickness is around 0.7mm to 2.0mm. (2) The structures of these root cortexes are closely similar, however, large number of latex tubes were existed in the inner cortex, primary and secondary phloem of Yong Chun Choo Woo. Well developed pith rays were also observed. (3) The other two varieties have less developed pith rays and many crystals of calcium-oxalate are observed. (4) The bast fibers are consisted of thick cell wall, and these are observed in these varieties, however, the fibers are not lignified. (5) The crystals in these root cortexes show solitary or polygonal shapes and these crysta ls can be considered as a complex of organic materials and calcium salt byy its solubility to mineral acids.

川芎類의 細胞分類學的 硏究

都貞愛 梨花女子大學校 韓國生活科學硏究院 1970 韓國生活科學硏究院 論叢 Vol.4 No.-

A comparative study of karyotype of Cnidium officinale Complex growing in Korea was carried out. The study shoes following:The somatic chromosome number of Ill Chun Gung was found to be 2N=22and that of Toh Chun Gung 2N=33,that of Toh Chun Gung(Ullung Island)2N=44 The karyotype of species Ill Chun Gung were as follows:the 22 chromosome were in 11 pairs,which could be classified into the 5 pairs of Submedium chromosome,4 pairs of Medium ones and 2 pairs of Subterminal of which one had satellite. The karyotype of Toh Chun Gung were as follows: the 33 chromosome were in 16 pairs one chromosome.8 pairs of Submedium chromosome,6 pairs of Medium ones and 2 pairs of Subterminal and one terminal chromosome. The karyotype of Toh Chun Gung (Ullung Island) were as follows:the 44 chromosome were in 22 pairs,13 pairs of Submedium,8 pairs Medium and 1 pairs of Subtrminal of which had satellite. In size of stoma and cell,Toh Chun Gung is strickingly larger than,Ill Chun Gung. The pollen ferility of Toh Chun Gung was zero,Ill Chun Gung was 1-3%. In the clone of Cnidium officinlae Makino growing in Korea,the karyotype was the orginal one,while,that of Toh Chun Gung presumably derives from the orginal karyotype.Toh Chun Gung is verified to be the polyploid in genus Cnidium. Ill Chun Ghun was found a diploid,and Toh Chun Gung was found a triploid,Toh Chun Gung(Ullung Island),was found a tetraploid.

天南星의 形態學的 硏究

都貞愛 梨花女子大學校 韓國生活科學硏究院 1978 韓國生活科學硏究院 論叢 Vol.20 No.-

Arisaema屬에 屬하는 種類들은 起原植物인 천남성 Arisaema amurense Max. var. serratum Nakai, 점백이천남성 Arisaema penimsulae Nakai, 두루미천남성 Arisaema heteraphlum Blume과 倍數體植物로 存在하는 種 그리고 基本種과의 混種으로 形態가 類似한 것으로 分類의 혼돈이 되고 있으며, 天南星 生藥中에도 Arisaema屬 種類등의 球莖으로서 또 各各의 雌雄株의 球莖으로서 混在되어 있음을 알 수 있다. 本 硏究는 光陵에 主로 많이 自生하는 種類로서 各各의 雌雄株의 分布狀態와 開花期의 各各의 器管을 比較 觀察한 결과 현저한 差異를 檢討하였다. 現在의 市販品 天南星 生藥中에는 Arisaema屬과 他의 Pinellia屬의 球莖이 混入되어 있어 이를 分類 鑑別하고자 本 實驗에서도 天南星球莖의 組織學的 內部構造와 細胞內含有物을 調査한 結果 Pinellia屬과 類似點이 있다고 보나 內部 形態學的으로는 鑑別이 可能하다고 思料된다. We explained that the crude drugs Arisaema on markets were confused numbers of the material which originated from the tuber of Arisaema species. This paper attempts to observe the morphological characteristics of dioecious of Arisaema species in Kwannung. The organs of the pistillate Arisaema are larger than those of the staminate Arisaema in size. The sexual identification is easy in the flowering season. The author carefully reviewed the histological structure of Arisaema. The observed results were follows: The microscopic study of tuber on transverse section is divided into external and internal parts by the ring of intercellular secretory sac. Raphides of calcium oxalate contained in mucilage duct. Collateral vascular bundle, are extremely similar to those of Pinellia. Most parenchyma cells of the inner parts were full filled with spherical starch grains in the cytoplasm. The starch grains divided into tow groups according to the staining PAS strong positive or weak positive.

난과식물의 생약학적 연구

도정애,Toh, Chung-Ae 한국생약학회 1994 생약학회지 Vol.25 No.3

In order to estimate accurate originality of the important crude drugs, Gastrodia, Dendrobium, Bletilla and Cremastra spp. were investigated comparing their morphological, anatomical and physicochemical characteristics and antibacterial, antifungal activities. The results of the studies as follows: 1. In morphological and anatomical studies, Korean Gastrodia contained more mucilage duct and symbiotic fungi than imported Gastrodia. Korean Dendrobium contained starch grains but without starch grains in the imported Dendrobium from China enclosed with thicken silicified wall. The corm of Cremasta appendiculata contained raphides of bundles with mucilage while the bulbs of Tulipa edulis contained several various starch grains form. 2. In physiological and TLC analysis, crude drugs in Orchidaceae contained common constituents with fluorescence and much mucilage. 3. The EtOH extracts of Gastrodia, Dendrobium, Bletilla, Cremastra showed antibacterial activities against B. subtilis and E. coli (Dendrobium>Gastrodia>Bletilla>Cremastra). But, no antifungal activities against C. albicans, A. niger were observed.

택사의 생약학적 연구

도정애,Toh, Chung-Ae 한국생약학회 1995 생약학회지 Vol.26 No.4

In order to clarify the originality of several Alisma Rhizomes, we investigated the differences of their morphological, anatomical and physiochemical characteristics. Morphological observation shows the variability of Alisma shape and quality. The diversities in morphological shapes and qualities of tuber were observed among different origins. Chinese product looked like corm shape, but korean's irregular shape. The microscopic observation of korean Alisma revealed the presence of irregular vascular bundle which was scattered transversely and longitudinaly, which was different from that of the rhizome. This facts suggest that the portion of crude drug in Alisma should be the corm rather than the rhizome. In the physicochemical differences, chinese Alisma contains larger amount of mineral elements such as K, Na and Ca than korean Alisma. On the contrary, protein contents in lyophilized powder of aqueous extracts in korean Alisma rhizome was significantly higher than chinese Alisma rhizome. The leaves and stems contained larger amount of Ca and Na than tuber, and may be used as the osmotic diuretics for chinese traditional medicine.

택사의 항균 및 항진균 작용에 관한 연구

도정애 梨花女子大學校 藥學硏究所 1997 藥學硏究論文集 Vol.- No.6

We previously reported the morphological, physiochemical characteristics of Al-ismae Rhizoma. In this study, we examined the antimicrobial and antifungal activities of 85% ethanol and water extracts of Alismae Rhizoma using disc method. Ethanol extracts of Korean Alismae Rhizoma showed antimicrobial activity on B. subtilis and S. aureus on the concentration dependent manner, whereas. Chinese Alismae Rhizoma revealed antimicrobioal activity on E. coli and N. gonorrhoeae. The water solution(500㎎/㎖) of each lyophilized powder of aqueous extracts didn't show any antimicrobial activity on B. subtilis, S. aureus and E. coli, on the contrary, they stimulated cell growth slightly. Ethanol extracts of Korean samples showed antifungal activity on A. niger, but not on C. albicans. Neither A. niger nor C. albicans were affected by Chineses samples.

택사의 항균 및 항진균 작용에 관한 연구

도정애,Toh, Chung-Ae 한국생약학회 1996 생약학회지 Vol.27 No.4

We previously reported the morphological, physiochemical characteristics of Alismae Rhizoma. In this study, we examined the antimicrobial and antifungal activities of 85% ethanol and water extracts of Alismae Rhizoma using disc method. Ethanol extracts of Korean Alismae Rhizoma showed antimicrobial activity on B. subtilis and S. aureus on the concentration dependent manner, whereas, Chinese Alismae Rhizoma revealed antimicrobioal activity on E. coli and N. gonorrhoeae. The water solution(500 mg/ml) of each lyophilized powder of aqueous extracts didn't show any antimicrobial activity on B. subtilis, S. aureus and E. coli, on the contrary, they stimulated cell growth slightly. Ethanol extracts of Korean samples showed antifungal activity on A. niger, but not on C. albicans. Neither A. niger nor C. albicans were affected by Chineses samples.