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都載喆,韓甫植,孫建鎬,金玉子 영남대학교 자원문제연구소 1983 資源問題硏究 Vol.2 No.-
An analytical study was carried out by means of TLC, IR and GC on the lipid components in the seed of Korean Xanthium strumerium and Sesamum indicum which was chosen for the comparison with the Xanthium strumerium. Total lipid content in chloroform-methanol extracts of Xanthium strumerium and Sesamum indicum were found to be 43% and 44%, respectively with triglycerides being major components. It was estimated that small amounts of 1,3-diglycerides, 1,2-diglycerides free fatty acids and sterols were also present in the extract. The Xanthium strumerium seed oil was found to contain myristic, palmitic, palmitoleic. stearic, oleic and linoleic acid, with the content of trace, 5.03, trace, 2.08, 16.98, 75.91%in total fatty acids, and trace. 4.36, trace. 2.34, 17, 14, 76.16% in triglycerides, respectively. On the other hand, the Sesamum indicum seed oil contained myristic, palmitic, stearic, oleic, linoleic, linolenic and arachidic acid, with the content of trace, 6.63, 3.91, 39.46,60.00%, trace and trace, respectively.
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도재철,정근영,손건호 영남대학교 약품개발연구소 1994 영남대학교 약품개발연구소 연구업적집 Vol.4 No.-
A flavone glycoside was isolated from the aerial parts of Cirsium nipponicum Makino in good yield and identified as pectolinarigenin 7-O-α-_(L)-rhamnopyranosyl (1→6)-β-_(D)-glucopyranoside, pectolinarin, on the basis of chemical and spectroscopic evidence.
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도재철,박노찬,장성준,조광현,박인화,손재권,김수웅 한국동물위생학회 1998 韓國家畜衛生學會誌 Vol.21 No.1
This study was carried out to identify the infection rate of common internal parasites according to the age and the incidence of single or mixed parasitic infections from outside rearing dog in Kyungbuk province. Fifty-one dogs in 4 dog rearing farms were examined, and fece samples of dog's were freshly collected from farm ground and rectum swabs. The results obtained from this study were summarized as follows ; 1. Out of 51 dogs examined, 9 dogs were infected with some helminths, and mean infection rate was 17.6%. 2. The frequently encountered helminths were Ancylostoma caninum and Trichuris vulpis (32.3%), Toxascaris leonina(16.7%), Dipylidium caninum(8.3%) and Isospora sp(8.3%), in order. Specially the prevalence of Toxascaris leonina was relatively high in this study than other reports, and its significance was discussed. 3. The infection rate in younger dogs was higher than that of older dogs, especially that of the dogs under 6 months old was 31.3%, 6 months to 1 year old dogs was 0.8%, and over 1 year old dogs was 18.2%, respectively. 4. Single infection rates with Ancylostoma cantnum, Trichuris vulpis, Toxascaris leonina, Dipylidium caninum and Isospora sp were 11.8%. But double infection rates with the Ancylostoma caninun plus Trichuris vulpis and Toxascars leonina was 5.8%.
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Rat에 caffeine 경구투여후 시간경과별로 혈액과 간조직에서 혈액화학성분, 지질 및 단백질 구성성분의 변화
도재철,허린수,Do, Jae-cheul,Huh, Rhin-sou 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4
This study was conducted to identify the effects of caffeine on the lipid and protein components or blood chemistry levels of the serum as well as the total homogenate, mitochondrial and microsomal fraction of the rat(Sprague-Dawley, female) liver. Acute test were conducted to determine those effects. The acute test was conducted by dividing rats into 7 groups according to the time lapsed after a single oral administration of 100mg/kg caffeine(that is control, 2hrs, 4hrs, 8hrs, 24hrs, 48hrs and 72hrs lapsed group). The concentrations of glucose, urea nitrogen, uric acid, creatinine, total protein, albumin, A/G ratio, triglyceride, total cholesterol, HDL-cholesterol, free fatty acid, phospholipid as well as the activities of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and alkaline phosphatase(ALP) were measured in the serum of each experimental groups. The concentrations of the carbonyl group, malondialdehyde(MDA) and the patterns of the SDS-PAGE(Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) were analyzed to determine the oxidative damages and metabolic changes on the lipid and protein components in the serum, and total homogenate, mitochondrial and microsomal fractions of the rat liver. The results obtained from this study were summarized as follows; 1. The concentrations of serum glucose were significantly higher(p<0.01) between 4(143.0mg/dl) and 8hrs(138.0mg/dl) in comparison to that of the control(101.1mg/dl) after a single oral administration of caffeine(100mg/kg). While on the other, there were no significant differences in the concentrations of urea nitrogen, uric acid, creatinine, total protein, albumin and albumin/globulin(A/G) ratio in comparison to those of the control. 2. The concentrations of total cholesterol and HDL-cholesterol in serum were significantly higher(p<0.01) between 4(77.4mg/dl, total cholesterol) and 8hrs(64.7mg/dl, HDL-cholesterol) in comparison to those of the control(62.8, 46.7mg/dl) after a single oral administration of caffeine(100mg/kg). On the other hand, the concentrations of triglyceride in serum were significantly lower(p<0.01) after 8hrs(38.8mg/dl) in comparison to that of the control(66.5mg/dl). 3. The activities of AST in serum was significantly higher(p<0.05) from 2hrs(149U/L) to 8hrs(178U/L) in comparison to the control(112U/L) after a single oral administration of caffeine(100mg/kg). The activities of ALT in serum were significantly higher(p<0.01) at 4(45.5U/L), 24(49.3U/L), 48(46.8U/L) and 72 hrs(42.3U/L) in comparison to that of the control(39.7U/L) after a single oral administration of caffeine(100mg/kg). On the other hand, there were no significant differences in the activities of ALP in comparison to that of the control. 4. The concentrations of free fatty acid in serum were significantly higher(p<0.01) at 8hrs(65.0mg/dl) in comparison to that of the control(37.6mg/dl) after a single oral administration of caffeine(100mg/kg). However, there were no significant differences in the concentrations of carbonyl group and malondialdehyde within serum, and liver homogenate, mitochondrial and microsomal fractions in comparison to that of the control. 5. The patterns of SDS-PAGE in serum, mitochondrial and microsomal fraction of the liver showed no significant differences.
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都載喆,韓甫植,孫建鎬 영남대학교 자원문제연구소 1982 資源問題硏究 Vol.1 No.-
Dried ana stripped firmiana plataifolio seeds were extracted with chloroform-methanol solvent system. The yield was ca. 37%. The extract was examined by means of TLC,GC and IR. The extract was seperated into seven fractions by means of preparative TLC. These fractions were found to be large amounts of triglyceridea (78%) and small amounts of sterol esters, free fatty acids, 1,2-diglyceriaes and β-sitosterol. The extract as a whole and triglycerides fraction were transesterified with sodium methoxide. All the acid component, free and bound of the extract as a whole and triglycerides fraction were identified as palmitic, palmitoleic, stearic, oleic and linoleic acid, and the contents as determined by GC analysis were 16.42 (15.69 in triglycerides), 0.74(0.84), 2.98(1.98), 14.93(12.44) and 59.73%(62.23%), respectively. 02 Sesame seeds were stored at different conditions, paper bag at room temperature and low temperature, controlled atmosphere (polyethylene bag) at room temperature and low temperature. Chemical analysis on the oil was carried out during storage. No significant increase in acid value was observed and saponification value also remained almost unchanged throughout the storage period, which means that the molecular structure of the triglyceride was almost intact. Saturated fatty acids increased during storage while unsaturated fatty acids decreased. The degree of the change from unsaturated to saturated fatty acids appeared to be high at room temperature and paper bag storage compared to low temperature and controlled atmosphere storage.
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배양세포주(MDBK cell)내에 caffeine, 철분 및 vitamin E 혼합처리시 배양시간 경과에 따른 지질과 단백질 구성성분의 변화
도재철,허린수,Do, Jae-cheul,Huh, Rhin-sou 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4
This study was conducted to identify the effects of caffeine or combinations of caffeine and iron or vitamin E on the lipid and protein components in the MDBK(Mardin-Darby Bovine Kidney) cells. For the In vitro test, MDBK cells in ${\alpha}$-MEM(Minimum Essential Medium) were divided into 4 treatment groups according to drug types and dosages as follows; the control(group A), group B was treated with 0.3mM caffeine, group C was treated with 0.3mM caffeine and 0.3mM ferric chloride, group D was treated with 0.3mM caffeine and 0.3mM vitamin E. Those groups were further divided into 5 subgroups according to the time lapsed(control, 4hrs, 8hrs, 24hrs and 48hrs lapsed group). The concentrations of the carbonyl group and malondialdehyde(MDA) and the patterns of the SDS-PAGE(Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) and fatty acid compositions were analyzed to determine the oxidative damages and metabolic changes on the lipid and protein components in the MDBK cells. The results obtained from this study were summarized as follows; 1. The concentrations of carbonyl group and malondialdehyde in MDBK cells of group C were significantly higher(p<0.01) in comparison to the control, and increased according to the time lapsed. But the results of groups B and D were little different in comparison to the group C. 2. As the analytical results of fatty acid compositions in MDBK cells, the proportions of palmitoleic acid and linoleic acid in groups B, C and D were lower in comparison to the control, while the proportion of arachidonic acid in groups B, C and D were significantly higher(p<0.01) in comparison to the control. 3. In order to determine the oxidative damages to the protein in MDBK cells, the patterns of the SDS-PAGE were examined and the patterns of SDS-PAGE in groups C and D were significantly different between 43kd and 200kd of molecular weight.
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Rat체내 Sulfamethazine 경구투여 후 시간경과에 따른 간장, 신장 및 근육내 잔류함유량 측정
도재철,Do, Jae-cheul 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
Sulfamethazine sodium was orally administrated to Sprague Dawley female rats(body weight: 200~250g) with the sonde caude at the dose of 20mg of sulfamethazine sodium per 100g of body weight for 3 days to investigate the depletion rate of the drug from liver, kidney and muscle of rat. The results obtained were summerized as follows; 1. The mean concentrations of sulfamethazine in liver according to the time lapsed after oral administration of the sulfamethazine sodium were decreased from 1.27ppm at day 1 to 0.28ppm at day 4. 2. Sulfamethazine concentrations in kidney according to the time lapsed after oral administration of the sulfamethazine sodium were decreased from 0.77ppm at day 1 to 0. 12ppm at day 4. 3. The mean concentration of sulfamethazine in skeletal muscle according to the time lapsed after oral administration of the sulfamethazine sodium was at or below 0.09ppm within 4 days after withdrawl of medicated solution.
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