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제연호(Yeon Ho Je),진병래(Byung Rae Jin),노종열(Jong Yul Roh),장진희(Jin Hee Jang),강석권(Seok Gwon Kang) 대한바이러스학회 1999 Journal of Bacteriology and Virology Vol.29 No.3
We have now constructed a novel recombinant baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) producing polyhedra with Bacillus thuringiensis (Bt) CryIAc crystal protein. The recombinant polyhedra produced by the recombinant baculovirus, Btrus, in insect cells was characterized. The recombinant baculovirus has two independent transcription units in opposite orientations with two promoters, p10 or polyhedrin gene promoter each initiating transcription of either native polyhedrin or fusion protein with polyhedrin and Bt CrylAc crystal protein. Surprisingly, this recombinant baculovirus stably produced recombinant polyhedra which were nearly similar to those of wild-type AcNPV. The immunogold staining experiment showed that the recombinant polyhedra were assembled with polyhedrin and Bt Cry1Ac crystal protein, and contained virus particles. Insecticidal toxicity of recombinant polyhedra of Btrus to the faU webworm, Hyphantria cunea, was strikingly improved in comparison with the wild-type AcNPV.
거세미나방속 해충에 독성을 가지는 Bacillus thuringiensis 균주의 분리 및 특성
장진희,노종열,제연호,이대원,우수동,설광열,강석권 한국잠사학회 1996 한국잠사곤충학회지 Vol.38 No.2
To isolate Bacillus thuringiensis toxic to Spodoptera species, we collected soil samples in Korea. In these samples, we characterized 7 B. thuringiensis isolates toxic to Spodoptera exigua or S. itura from soil, granary and sericultural farm samples. The 7 isolates were named B. thuringiensis STB-1,STB-2, STB-3, STB-4,STB-5, STB-6 and STB-7, respective. The bioassay of these islates against S., exigua and S.litura showed highly insecticidal activity. The serotypes of them were determined by agglutination tests wsing 33 antisera ; STB-1 and STB-2 are identical to B. thuringiensis subsp. Kurstaki, and STB-3, STB-4 and STB-5 are identical to subsp. Kenyae STB-6 and STB-7 did not react with 33 antisera. STB-1 and STB-3 which have different gene types from B. thuringiensis subsp. Kurstaki and subsp. Kenyae are identified new isolates. STB-6 and STB-7 which show no agglutination in serological tests have cryLA(a), cryLA(b), cryIC, and cryII genes are also identified new isolates. Molecular weights of parasporal inclusions of all isolates were determined approximately 130 kDa by SDS-polyacrylamide gel electrophoresis.