HPLC를 이용한 건강기능식품 중 코엔자임 Q10 성분 분석

노기미,정자영,임동길,김미경,박경식,윤태형,홍진,박선영 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.1

A method based on high-performance liquid chromatography (HPLC) with ultraviolet detection has been developed to quantify coenzyme Q10 (CoQ10) in raw materials and dietary supplements. Single-laboratory validation has been performed on the method to determine linearity, selectivity, accuracy, limits of quantification (LOQ) and repeatability for CoQ10. An excellent linearity (r = 0.999) was observed for CoQ10 in the concentration range 15.625~500 μg/mL in dietary supplement. Observed recovery of CoQ10 was found to be between 98.33 and 99.38%. LOQ was found to be 250 μg/mL Repeatability precision for CoQ10 was between 0.15 and 0.21% relative standard deviation (RSD). Further, limited studies showed that some adulterants and degraded material could be satisfactorily separated from CoQ10 and identified.

국내 유통 주류 중 중금속 실태조사

노기미,강경모,백승림,최훈,박성국,김동술 한국식품위생안전성학회 2010 한국식품위생안전성학회지 Vol.25 No.1

This study was conducted to estimate the contents of heavy metals including lead, cadmium,arsenic and total mercury in alcoholic beverages in Korea. Concentration of Hg was analyzed by gold amalgamation method, using mercury analyzer, while concentration of Pb, Cd and As was analyzed by ICP-MS. Concentration (μg/kg) of heavy metal in fermented liquors were ; for Pb 9.9 ± 8.4(0~38.0), Cd 5.8 ± 4.9(0~5.4), As 28.6 ± 19.4(1~96.4),Hg 0.7 ± 1.2(0~10.6). Concentration (μg/kg) of distilled liquors were : for Pb 4.4 ± 5.7(0~29.3), Cd 2.0 ± 2.5(0~10.3),As 12.0 ± 17.0(0~95.6), Hg 0.2 ± 0.3(0~2.3). Concentration(μg/kg) of other liquors were ; for Pb 7.5 ± 5.1(0~13.7),Cd 5.8 ± 3.9(0.6~11.2), As 25.2 ± 39.0(0.5~103.3), Hg 0.3 ± 0.1(0.1~0.5). The present study showed that difference of the amount of constituent in a same category of food are not affect to the content of heavy metals among them. The residual levels of takju, yakju, sake, beer, fruit wine, soju, whiskey, brandy, general distilled liquor, liquor, other liquors are within the maximum levels, prescribed by Korea food code. It is given that heavy metal exposure of Pb,Cd, As, Hg from consumption of alcoholic beverages (takju, yakju, sake, beer, fruit wine, soju, whiskey, brandy, general distilled liquor, liquor, other liquors) are less than 0.03%, 0.06%, 0.01%, 0.01% (mean) in provisional tolerable weekly intake (PTWI) respectively, indicated by FAO/WHO.

건조생강의 감마선 조사에 의한 휘발성 향기성분 변화

노기미(Ki-M No),서혜영(Hye-Young Seo),Rajendra Gyawali,심성례(Seong-Lye Shim),양수형(Su-Hyeong Yang),이성진(Sung-Jin Lee),김경수(Kyong-Su Kim) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.6

본 연구에서는 세계적으로 널리 이용되고 있는 건조 향신료 중 건조생강의 감마선 조사에 의한 휘발성 향기성분의 변화를 관찰하였다. 비조사 건조생강과 10 k㏉의 선량으로 조사처리된 건조생강을 SDE방법으로 휘발성 성분 추출후 GC/MS로 분석하였다. 비조사 시료와 조사시료에서 각각 83종, 71종의 화합물이 분리 동정되었으며, 감마선 조사에 따른 뚜렷한 상관관계는 없었다. 건조생강의 주된 향기성분으로는 α-zingiberene, β-sesquiphellandrene, geranial, (Z, E)-α-farnesene, β-phellandrene으로 이는 조사 처리된 건조생강의 휘발성 향기성분의 조성과 유사함을 알 수 있었다. 방사선 비조사 및 조사 처리된 정유성분의 총량은 각각 98.27%에서 98.12%로 0.15% 감소하였으며 monoterpene은 17.15%에서 17.90%로 미량 증가하였으며, sesquiterpene은 60.99%에서 61.24%로 증가하였다. 반면 oxygenated monoterpene과 oxygenated sesquiterpene은 각각 0.5%와 0.65% 감소되었다. 성분들 중에서 감마선 조사에 따라 α-zingiberene, β-sesquiphellandrene, geranial, (Z,E)-α-farnesene, β-phellandrene 등의 함량이 감소됨을 알 수 있었지만 이는 소량으로 감마선 조사가 건조생강의 관능적 요소에 미치는 영향으로 보기는 어렵다. The effect of gamma irradiation on volatile components of Korean dried ginger (Zingiber officinale Roscoe) was studied and compared with non-irradiated sample. Volatile compounds from non- and irradiated samples were extracted using simultaneous distillation-extraction (SDE) apparatus and analyzed by gas chromatography-mass spectrometer (GC/MS). A total of 83 and 71 compounds were identified and quantified from non- and irradiated dried ginger at dose of 10 k㏉. Identified components were hydrocarbons, alcohols, aldehydes, esters, ketones and miscellaneous compounds. The terpenoid compounds in volatile flavor compounds identified from non and irradiatied dried ginger were 98.27% and 98.12%, respectively. α-zingiberene, β-sesquiphelland rene, geranial, (Z,E)-α-farnesene, β-phellandene were detected as major volatile compounds of two experimental sample. The amount of volatile components in the samples was changed by irradiation but the profile of volatiles in non- and irradiated dried ginger were the same.

전자선 조사와 저장기간에 따른 포도의 휘발성 유기성분 변화

심성례,정인선,노기미,김준형,권중호,김경수 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2009 한국식품저장유통학회 학술대회 Vol.2009 No.-

건생강에 함유된 키랄성 향기성분의 이성질체 조성 분석

서혜영(Hye-Young Seo),노기미(Ki-Mi No),심성례(Seong-Lye Shim),류근영(Keun-Young Ryu),한규재(Kyu Jae Han),라젠드라 게왈리(Rajendra Gyawali),김경수(Kyong?Su Kim) 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.7

건생강 제품의 진위판별을 위한 기초자료를 제공하기 위하여 건조된 생강으로부터 휘발성 향기성분을 추출하여 향기특성을 분석하고, MDGC/MS에 의해 키랄성 휘발성분의 enantiomeric composition을 분석하였다. 건생강에서 총 57종을 동정하였으며 주요 휘발성 향기성분은 zingiberene, β-sesquiphellandrene, β-bisabolene, (E,E)-α-farnesene 및 α-curcumene이었다. 건생강에 함유된 키랄성 향기성분 중에 서 enaniomeric composition을 측정하기 위해 선택된 키랄화합물 중 α-pinene 및 nerolidol은 높은 enantiomeric purity(>96%)로 검출되었으며, β-pinene은 (R)-enantiomer 만이 검출되어 특이적이었다. α-Terpineol은 (R)-enantiomer가 72%로 높게 나타났으며 linalool과 4-terpineol은 두 이성질체가 혼합된 것으로 나타났다. Limonene의 enantiomeric excess(ee, %)는 17.2%로 (S)-enantiomer가 주요 이성질체이었다. 따라서 이러한 키랄성 향기성분의 enantiomeric composition은 건생강의 진위평가를 위한 변수로 사용될 수 있을 것으로 생각된다. The volatile compounds of Zingiber officinale Roscoe were extracted by simultaneous steam distillation and extraction (SDE) method and identified with gas chromatography/mass spectrometer (GC/MS) analysis. Enantiomeric compositions of chiral compounds were determined by multidimensional gas chromatography/ mass spectrometer (MDGC/MS). A total of 57 compounds were identified and quantified, including zingiberene, β-sesquiphellandrene, β-bisabolene, (E,E)-α-farnesene and α-curcumene. Among them, zingiberene (38.41%) was found as the predominantly abundant component. α-Pinene and nerolidol in dried ginger were detected by high enantiomeric purity (>96%) for (S)-form, and β-pinene was detected only (R)-form. The enantiomeric composition of α-terpineol revealed 72.0% for (R)-form, and linalool and 4-terpineol showed mixtures of both enantiomers. (S)-Enantiomer was the major enantiomer of limonene having enatiomeric excess of 17.2%. Hence the enantiomeric composition of these compounds can be used as parameter for authenticity control of Zingiber officinale.

신 제형 클로렐라제품의 총 엽록소 시험법 개선

김유경,박영식,이은숙,한재갑,노기미,임동길,정자영 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.1

A new and improved analytical method involving alkaline pyridine extraction was proposed to quantity chlorophyll contents in syrup and candy type chlorella products. The performance of analytical method was compared with the conventional Korea food standard method which involves acetone extraction. The application of sonication chlorophyll extraction form alkaline pyridine sample was also explored. The analytical procedure was validated by evaluating accuracy, precision and reproducibility. For liquid samples, the pyridine extraction method showed higher accuracy and precision compared to acetone extraction method. The CV values of pyridine extract method and the acetone extraction method were 18.82 and 40.0, and the accuracy to theoretical values were 106.3%and 78.1%, respectively. When sonication extraction method was applied to the pyridine extraction, the precision was improved as indicated by reduced CV values from 18.82 to 11.36. The improved performance of pyridine-sonication extraction was also validated by recovery test of chlorophyll that was previously spiked into the sample matrix. For solid matrix, the pyridine extraction method showed better performance in analysis of chlorophyll in solid food matrix (CV = 7.05) compared to conventional acetone extraction method (CV = 30.0). However, the accuracy to theoretical values of pyridine and acetone extraction methods only showed only 62.7% an 40%, respectively. The relatively low accuracy of pyridine extraction method (62.7%) was improved to 99.4% by applying additional sonication extraction method. The improved performance of applying additional sonication extraction was validated by standard deviation,CV values and accuracy to theoretical values.

전자선 조사한 캠벨얼리 포도(Vitis Labrusca L.)의 휘발성 유기성분 변화

심성례 ( Sung Lye Shim ),노기미 ( Ki Mi No ),김경수 ( Kyong Su Kim ),송기동 ( Gi Dong Song ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2010 한국식품저장유통학회지 Vol.17 No.1

Monitoring of illegally added drugs in adulterated foods

임무송,김형일,강혜순,김은주,김미옥,노기미,신용우,김민석,양지영,장은정,조중훈,임철주,최선옥 한국분석과학회 2016 학술대회논문집 Vol.2016 No.11

Risk Assessment for Heavy Metals in Korean Foods and Livestock Foodstuffs

권영민,이경희,이행신,박선오,박정민,김진만,강경모,노기미,김동술,이종옥,홍무기,최달웅,Kwon, Young-Min,Lee, Kyoung-Hee,Lee, Haeng-Shin,Park, Seon-Oh,Park, Jung-Min,Kim, Jin-Man,Kang, Kyung-Mo,No, Ki-Mi,Kim, Dong-Sul,Lee, Jong-Ok,Hong, Moo- Korean Society for Food Science of Animal Resource 2008 한국축산식품학회지 Vol.28 No.3

This study was conducted to evaluate exposure level and risk of heavy metals in livestock foodstuffs and Korean foods. Based on the "Food Intake Data," a part of the 2005 National Health & Nutrition Survey and the "2005 Seasonal Nutrition Survey", 113 Korean foods items were selected. 3 samples from different manufacturers of each 113 items of Korean foods were purchased on summer and fall, so total 678 samples were used. The food groups were classified into 15 categories. For the livestock foodstuffs category, meats and poultry (chicken, pork, pork belly, beef, beef feet soup), milks and dairy products (milk, ice cream, liquid yoghourt, sherbet), eggs (egg) were selected. It was found that the daily amount of heavy metals intake (mg/person/day) from livestock foodstuffs is 0.00020 arsenic, 0.00000 cadmium, 0.00020 lead, and 0.00006 mercury, and the daily amount of heavy metals intake (mg/person/day) from Korean foods is 0.0265 arsenic, 0.0083 cadmium, 0.0067 lead, and 0.0028 mercury. Daily amount of heavy metals intake from livestock foodstuffs was low among the food groups. For risk assessment, PDI (Probable Daily Intake) was calculated and compared with PTWI (Provisional Tolerable Weekly Intake) of JECFA (Joint FAO/WHO Expert Committee on Food Additive). Relative hazard of these livestock foodstuffs was 0.006% in arsenic, 0.000% in cadmium, 0.085% in lead, and 0.149% in mercury. Relative hazard of Korean foods was 0.941% in arsenic, 14.676% in cadmium, 3.319% in lead, and 6.860% in mercury. Thus, livestock foodstuffs and Korean foods were as safe as satisfied with the recommended standards of JECFA.

음식점 식육 원산지 표시 모니터링

홍진,임동길,김미경,박경식,윤태형,노기미,정자영 한국식품위생안전성학회 2010 한국식품위생안전성학회지 Vol.25 No.2

The compulsory beef labelling system has launched from January 1st 2007 by the amended Food Hygiene Law, we were checked the actual conditions of beef origin with a nationwide scale by the Hanwoo differentiation specific test method which was developed by Korea FDA using 90 SNP biomarkers. The test method is useful tool to differentiate the beef origin carrying out the mission of KFDA's annual food safety management guidance. Also we have technically transferred the Hanwoo differentiation specific test method to other institutes as well regional KFDA and established the training program as a regular course in Korea Human Resource Development Institute for Health and Welfare. The beef used in this study were collected according to the 2009 Food safety guidance in roast beef restaurants where business site area greater than 100 m2. Total 216 samples were consisted of 48samples of the Seoul area and 168 of the region. The monitoring result from restaurants in all the region of Korea showed that 3 of 216 Hanwoo-labelled beefs were found out as a non-Hanwoo (1.3%). This results are gradually deceasing trend compared with 34.0% in 2005, 30.1% in 2006, 3.2% in 2007 and 5.14% in 2008. From these data, the Hanwoo differentiation specific test method on the settlement of the compulsory beef labelling system has an important role. As a outcome of this project, we might be considered the early settlement of the compulsory beef labelling system, technically transferred to other institutes and the establishment of regular training program of the test method.