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김학산,남홍우,민경완,송형운,정경은 대한소화기학회 1991 대한소화기학회지 Vol.23 No.4
Spontaneou bacterial peritonitis is a serious problem in patients with cirrhotic ascites, with an incidence of 10% to 27% in hospital admission and mortality over 40%. The conventional method of ascitic fluid culture has a low sensitivity in detecting causative organism of spontaneous bacterial peritonitis. We have prospectively 1) compaired the sensitivity of two culture methods (conventional vs blood culture bottle method with thioglycollate broth) in cirrhotic patients with spontaneous bacterial peritonitis, 2) determined the optimal inoculation volume of ascites, 3) identified the causative organism of SBP. During the 12 month period,$quot;twenty two episodes of SBP occured in 46 patients with cirrhotic ascites. The conventional method grew an oirganism in 5 episodes whereas the blood culture bottle method did in 16 episodes (22.7% VS 72.7%, $lt; 0.05). Ten-mililiter inoculation of ascites into a blood culture bottle were superior to 5-mililiter inoculation in detecting organism, but not significantly (72.7%(59.0%, p$gt;0.05). We concluded that the inoculation of ascitic fluid to a blood culture bottle is more sensitive than the conventional method and shoule be used routinely for ascitic culture in cirrhotic patients.