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      • 갑상선암에서 MAGE 1-6 mRNA를 검출하는MAGE common primer의 의의

        남철우,최영식,박요한,이강대 고신대학교의과대학 2006 고신대학교 의과대학 학술지 Vol.21 No.2

        Background: MAGE (melanoma antigen gene) was named as cancer/testis specific antigens since their expression is only detected in the testis or cancer cells. Because of their relatively specific expression in the cancer cells, they have been considered as the early diagnosis of several cancers, or the appropriate targets for the specific immunotherapy mediated by cytotoxic T lymphocytes. Therefore, there were many reports about the expression of MAGE genes in various types of malignant tumors, but only a few reports in human thyroid neoplasms. The purpose of this study was to determine whether the MAGE common primers expressed in different histological types of thyroid tumors and to see the clinical usefulness of MAGE common primers on the thyroid tumors. Methods: Fifty-nine patients who underwent thyroidectomy at Kosin Medical Center from January, 2001 to April, 2002 were included in the study. Of the 59 patients 40 were papillary carcinoma, 4 follicular carcinoma, 3 medullary carcinoma, 1 anaplastic carcinoma, 4 nodular hyperplasia, 3 adenomatous goiter, and 4 follicular adenoma. Total cellular mRNA was extracted from those 48 cancer tissues, 11 benign tissues and additional eleven normal control thyroid tissues. Nested reverse transcriptase-polymerase chain reactions (nested RT-PCR) with the MAGE common primers designed to detect MAGE 1-6 genes were employed in this study. With probes confirmed by DNA sequencing. Results: Of those 48 cases of thyroid carcinomas 10 cases (20.8%) were expressed MAGE common primers. In the 40 papillary carcinoma, MAGE common primers were expressed in 17.5%. In the four follicular carcinoma, they were expressed in two cases, and in one case of one anaplatic carcinoma. However, in the three cases of medullary carcinoma, MAGE gene was not expressed. None of 11 benign tumors and 11 normal tissues expressed any of these MAGE common primers. The sensitivity of MAGE common primers in thyroid tumors was 20.8% and the specificity was 100%. Conclusion: These results suggested that MAGE common primers were expressed low in the malignant thyroid tumors but it was not expressed in benign tumors and normal tissues. It is suggested that MAGE common primers can be used for preoperative differential diagnosis of follicular carcinoma from benign follicular adenoma which cannot be made by fine needle aspiration cytology of thyroid tissue. However, further study is needed for clinical application of MAGE common primers expression as tumor markers in the thyroid tumors.

      • KCI등재

        (Cu-Ni-Co-Fe)계 매트의 고온고압 황산침출연구

        南哲祐,박경호,金洪仁 한국자원공학회 2004 한국자원공학회지 Vol.41 No.5

        망간단괴로부터 제조되는 매트상과 유사한 합성 (Cu-Ni-Co-Fe)매트상을 제조하여 고온고압하에서 황산에 의한 구리, 니켈, 코발트 그리고 철의 침출특성을 조사하기 위해 황산농도, 반응시간, 반응온도, 산소압이 유가금속들의 침출율에 미치는 영향을 검토하였다. 고액농도 25g/500㎖에서 황산농도 1.0%(V/V), 산소압력 10 ㎏/㎠, 반응온도 150 그리고 반응시간 이 2시간의 경우 니켈, 코발트 그리고 구리 모두 98% 이상 침출되었으며 철은 1.4%가 용해되었다. 황산농도가 1.0% (v/v)보다 높으면 침출액의 pH가 낮아져 철의 침출율이 급격히 증가하였다. 니켈과 코발트의 침출율은 침출온도와 산소압력에 별 영향을 받지 않으나 구리는 큰 영향을 받았다. The sulfuric acid pressure leaching of artificial matte which was similar to the matte prepared from manganese nodules was carried out to investigate the extraction behaviors of copper, nickel, cobalt and iron. The effects of sulfuric acid concentration, leaching time, temperature and oxygen pressure were studied. The extractions of copper, nickel and cobalt were over 98% by 10kg/cm2 oxygen pressure leaching with 1.0%(v/v) H2SO4 and 25g/500ml pulp density at 150 , 2 hours. At this condition, iron was extracted only 1.4% with precipitated as hematite. The leaching of iron increased with increase of H2SO4 concentration due to high acidity of solution. The leaching temperature and oxygen pressure give little effects on leaching of nickel and cobalt while great on copper.

      • 제6차와 제7차 교육과정에서 초등학교 과학 교과서 삽화 비교 분석 : Centered on the 3rd Year Class of the Elementary School

        남철우,권영길 光州敎育大學校 初等敎育硏究所 2002 初等敎育硏究 Vol.17 No.1

        This study was intended to examine the difference and the suggestion through comparative analysing the illustrations in the 3rd elementary science textbooks of the 6th with the 7th curriculum. The findings of this research were as follows. (1) Compared with the 6th curriculum, 448 more illustrations are presented in the 7th curriculum science textbook and the teacher's teaching techniques should be, therefore, improved the using ability of the illustration in the science education. (2) Although more than 96% of the illustrations are those of photographs and pictures in the 6th curriculum, those in the 7th consist of many kinds of the illustrations-photographs, picture, diagrams and cartoons. Especially, the more frequent introductions of the cartoons in the textbook give familiarity to the students, and it suggests that the textbooks were constructed in consideration of the student's intellectual development. (3) The roles of illustrations in the 6th and the 7th curriculum are mostly guidance for experiments and the supply of the learning materials. but considering that the learning motivation is very important in the teaching-learning process. the percentage of the illustrations in accordance with the motivations must be increased, (4) As the result of the analysis of illustration in the each domains, in the 6th curriculum there are the most numbers of illustrations in the biology domain and the least in the physics domain. But contrastively in the 7th curriculum there are the most in the energy and the least in physics, and namely those are distributed widely. (5) Analysing the kinds of illustrations in the each domains, in the both sixth and seventh curriculum the photographs are used in the physics mostly, and it is, therefore, interpreted because of the reason that the more realistic material supply should be needed. (6) According to the roles of illustrations in the each domains, there are the many illustrations for guidance of the experiments in the both curriculum, and contrastively in the biology part and the earth science, the many illustrations are introduced for material supply. In this viewpoint it is known that the illustrations of the physics domains and the energy are constructed for experiment, and those of the biology domain and earth science for investigation and observation of the given materials. (7) the average numbers of illustrations to be taught in a period or in a page are introduced more twice in the sixth text-book than the seventh. It is, naturally, concluded that the teachers' training process for using the illustrations is needed urgently.

      • KCI등재

        GaAs 웨이퍼 스크랩에서 갈륨 침출에 관한 연구

        남철우,이효숙,홍성웅,원창환 대한금속재료학회(대한금속학회) 1995 대한금속·재료학회지 Vol.33 No.2

        Gallium is a relatively rare element and its principal use in the manufacture of semiconducting compounds, mainly GaAs. Consequently, a large increase in the demand for gallium is anticipated in the future. Recycling of GaAs wafer scrap is required to solve the environmental problems caused by arsenic and to reutilize gallium which is an expensive metal. To recovery of gallium from the GaAs scrap, the basic study on the gallium leaching was performed with varying leaching agents and conditions. The important results were obtained such as followings; Nitric acid was the most effective leaching agent in comparison with HCl and H₂SO₄. More than 99% of Gallium was leached at conditions; 2N HNO₃, 500RPM, -100+200 mesh and at 60℃. The apparent activation energy of the leachuig reaction was 93.05 KJ/mole. The total reaction rate of this reaction was as follow : [dGa(III)/dt]_i=k′[GaAs]^(2.32)·[HNO₃]^(3.20)·Exp(-93.05/RT)

      • KCI등재

        교과교육 사조의 고찰에 따라 새로 권장되는 교수학습방법

        남철우 한국초등과학교육학회 1999 초등과학교육 Vol.18 No.1

        This paper describes some major trends in primary and secondary education now. It is a selective views of significant topics with important implication for the future. These trends are summarized as follows, after examining historical development of Teaching-learning Methodology in primary and secondary education. 1. Education is seen as part of interdisciplinary world; emphasis is on relating education to the students' world, which is not compartmentalized. 2. Emphasizing students' individual growth with their own goal of lives. 3. Open-education with variable contents and purposes of education. 4. Education for all from that for some. 5. Consructivist based from behavior based. 6. Cooperative learning, peer tutoring, group projects. 7. Authentic assessment; assessment integrated with instruction. 8. The teacher is a facilitator of learning and a learner as well; students are learners and teachers in some situations; networks emerge instead of one-way forms or communication. 9. Spiral curriculum from single exposure.

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