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大麥 latent β=amylase의 活性化機構에 關한 硏究
南昌祐 同德女子大學校 1975 同大論叢 Vol.5 No.1
The activation mechanism by papain of latent β-amylase in barley was reinvestigated by using several proteolytic enzymes and 2-mercaptoethanol. The results optained were as follows: 1) Although papain has so far been regarded as one of the most effective reagents for activation of latent β-amylase in barley and therefore employed for the determination of the total β-amylase in barley seeds. But, during the course of investigation on extraction of β-amylase from ground barley, 2-mercaptoethanol was found to be more effective than papain and to be roble to activate latent β-amylase completely in the coexistence with papain. 2) At the same time, on the basis of the experimental results obtained during the extraction of barley β-amylase by the use of 5% potassium sulfate, 0.2% papain, 0.2M 2-mercaptoethanol and 0.2M 2-mercaptoethanol containing U.2n papain, the possibility was suggested that there were soluble but inactive types of β-amylase in the salt and papain extracts which were activated by the treatment with 2-mercaptoethanol, Their fluctuations during barley germination were also observed. 3) Trypsin and pepsin as T·ell as papain were effective in solubilizing and activating latent β-amylase, but the boiled preparations had no effect on the activation. From these results it was thought that there would be two kinds of activation mechanisms; (1) splitting of peptide bonds with proteolytic eilzymes, and (2) splitting of disulfide bonds with thiol compounds. 4) The total β-amylase activity determined whth 2-mercaptoethanol containing papain was found to be affected neither with nor without the gibberellic acid treatment during germination. Though the amount of active β-amylase in malt produced by normal germination did not reach to that of total β-amylase determined with 2-mercap- toethanol containing papain, the gibberellic acid treatment of barley was found to enhance the activation of latent β-amylase in vivo which resulted in the Increase in tile papain-soluble active β-amylase activity up to that of total β-amylase. From these results it is quite possible that all the types of latent β-amylase are activated by devices on malting method and that the increase in active β-amylase activity during germination is exclusively due to the activation of latent β-amyase formed during ripening and dormancy of barley seeds.
집토끼 신장 Soluble Form Trehalase의 정제 및 성상에 관한 연구
南昌祐 同德女子大學校 1984 同大論叢 Vol.14 No.1
Trehalase (α,α-trehalose I-D-glucohydrolase, EC 3.2. 1.28) responsible for the hydrolysis of trehalose was isolated and purified from rabbit kidney. The activity of trehalase in rabbit kidney homogenate was 232.08 units Per gram and detected 25.29% in cytosolic fraction, 44.48U in mitochondrial fraction and 30.23U in nucleic fractlorl. A soluble form of the enzme was purified from the cytosolic fraction. It was purified about 100-folds by ammonium sulfate precipitation, DEAE-cellulose column chromatography and Sephadex G-200 gel filtration. The soluble form trehalase was completely specific for trehalose as substrate. None of the other glucose disaccharides eihibited any significant activity. The product of the reaction was identified as D-glucose by paper chromatographic method. The optimum pH of the enzyme was 6.0 in phosphate buffer and shifted to 5.2 in acetate buffer and optimal temperature was 55 and the Km value for trehalose was estimated to be about 5.0 mM. The enzyme activity was activated as much as 10% by Na+ and K+ at the concentration of 1×10-3 M, and 36% by 1×10-4 M of Mg++. Its activity was inhibited 32% by 1×10-3M p-chloromercuribenzoate. The molecular weight of soluble form trehalase was estilnated to be approximately 70,000 by Sephadex G-200 gel filtration.
NMMO/물 용매계에서 셀룰로스/N-(2-하이드록시)프로필-3-트리메틸암모늄 키토산 클로라이드 블렌드 섬유의 제조
남창우,고석원,최재원,김영호 한국섬유공학회 2003 한국섬유공학회지 Vol.40 No.6
Cellulose and N-(2-hydroxy)propyl-3-trimethylammonium chitosan chloride (HTCC), a water-soluble chitosan derivative synthesized by the reaction of chitosan and glycidyltrimethylammonium chloride, were blended in solution and the method to prepare blend fibers using them was investigated. Various blend solutions with different HTCC contents (up to 20%) were prepared using 50% N-methylmorpholine-N-oxide (NMMO) aqueous solution as a co-solvent and the cellulose/HTCC blend fibers were obtained via dry-jet/wet spinning process. Some of quaternary ammonium groups in HTCC were lost and converted to primary amino groups during the dissolution and spinning process. The blend solution was brownish and transparent and no phase separation was observed. Moreover, SEM showed that HTCC component was distributed uniformly in cellulose. The blend fibers showed excellent antimicrobial activity even at very low content (1.0%) of HTCC.
Nanoporous Pt Thin Films with Superior Catalytic Activities by the Electrochemical Dissolution of Al
남창우,김천중,박병우,박예준 대한금속·재료학회 2009 METALS AND MATERIALS International Vol.15 No.6
Thin films of alloyed PtxAl1-x with various chemical compositions (x ≅ 0.27, 0.52, and 0.76) were obtained by a co-sputtering method. As cycles proceeded, PtxAl1-x thin films showed gradually increasing electrochemical surface areas (Pt-H), due to the electrochemical dissolution of Al from PtxAl1-x thin films, and this reaction led to the straightforward formation of Pt nanoporous structures. Among three different chemical compositions, Pt0.52Al0.48 thin films satisfied both enhanced catalytic activity and mechanical stability after cycling. Electrochemically fabricated nanoporous Pt thin films exhibited superior methanol-oxidation activities compared with untreated Pt and PtRu thin films.
국내·외 유·아동 디지털 교육 동향과 디지털 교육 발전 방안 탐색
남창우 육아정책연구소 2021 육아정책포럼 Vol.69 No.-
디지털 전환으로 인한 유·아동의 디지털 접근성 향상은 뉴노멀을 맞이할 것으로 전망된다. 한편, 잦은 디지털 접촉으로 인하여 제기되는 부작용으로 인하여 유·아동은 물론 유·아동을 둘러싼 인프라 전반에 대한 개혁이 요구된다. 본고에서는 디지털 리터러시 함양을 목적으로 수행된 디지털 교육 관련 연구와 동향을 탐색함으로써, 디지털 교육의 현황을 파악하고 유·아동 디지털 교육 혁신의 구체적인 아이디어를 제안하고자 하였다. 이에 국내·외 디지털 교육 관련 문헌을 분석하여, 공통적으로 제안되는 함의점을 기반으로 몇 가지 제언을 제시하였다. 첫째, 향후 유·아동 디지털 리터러시를 함양을 위한 부모 및 보호자의 태도를 환기할 필요가 있다. 둘째, 디지털 교육의 확장 및 접근성을 위하여 지역사회 특히 대학과 연계된 통합 시스템 도입이 요구된다. 마지막으로 유·아동 디지털 리터러시 역량 연계된 부모 디지털 교육 체제 수립도 정책적으로 연구 및 사업이 진행될 필요가 있다.
Social Welfare Analysis of Policy-Based Finance with Support for Corporate Loan Interest
남창우 한국개발연구원 2021 KDI Journal of Economic Policy (KDI JEP) Vol.43 No.4
We analyze the social welfare effect when a policy-based financial system (PFS) enters a decentralized financial market. Particularly, the PFS in this case supports the interest spread for corporate loans held by firms with heterogeneous bankruptcy decisions under an imperfect information structure. Although support for capital costs through the PFS expands the economy consistently, the optimal level of PFS out of the corporate loan market is estimated to be 8.6% by a simulation model considering social welfare adjusted by the disutility of labor. This result is much lower than the recent level of PFS in the Korean financial sector.
대학생의 ICT 자아효능감과 플립러닝에 대한 인식 간의 관계 탐색
남창우,박영희,권종실 부산대학교 교육발전연구소 2017 교육혁신연구 Vol.27 No.2
연구목적: 본 연구는 대학교육에서 대학생들의 ICT 자아효능감과 플립러닝에 대한 인식 간에 어떠한 관계 가 있는지 탐색하였다. 연구방법: 이를 위해서, 본 연구는 부산지역의 4년제 D대학교의 교육공학 관련 과 목을 수강하는 대학생 120명을 대상으로 플립러닝에 대한 오리엔테이션을 진행하였다. 또한, 이들을 대상으 로 플립러닝에 대한 인식 검사와 ICT 효능감 검사를 진행하고, 이를 분석하였다. 연구결과: 주요 연구결과 를 살펴보면, 첫째, 대학생의 플립러닝에 대한 인식과 ICT 자아효능감 간에는 통계적으로 유의미한 상관관 계가 있는 것으로 나타났다. 또한, 두 변인의 하위 영역 간의 관계에 있어서는, 플립러닝에 대한 인식의 하 위 변인 중에 ‘온라인 수업에 대한 선호도’와 ‘자기주도학습기반 ICT 효능감’이 정적인 상관관계를 나타냈 다. 둘째, 대학생의 플립러닝에 대한 전체적인 인식은 ICT 자아효능감의 2가지 하위 요인인 ‘자기주도학습 기반 ICT 효능감’과 ‘스캐폴딩기반 ICT 효능감’으로 설명된다는 연구 모형은 유의미한 것으로 나타났다. 셋 째, 토론 강좌의 수강 경험이 많을수록 ICT 자아효능감의 하위변인인 ‘자기주도학습기반 ICT 효능감’과 ‘스 캐폴딩기반 ICT 효능감’이 모두 대체로 비례해서 높은 것으로 나타났다. 결론: 이와 같은 결과는 대학교육 에서 플립러닝을 본격적으로 도입할 때, 학습자의 이전 학습 경험과 ICT 효능감 수준 등 학습자 특성을 고 려한 개별화된 맞춤형의 교수설계 및 지원 방안을 마련할 필요가 있다는 것을 의미한다.
The Effects of Financial Support Policies on Corporate Decisions by SMEs
남창우 한국개발연구원 2016 KDI Journal of Economic Policy (KDI JEP) Vol.38 No.3
This paper investigates the effectiveness of public credit guarantee programs and interest-support programs for SMEs (small and medium enterprises). First, assuming that there is an imperfect information structure in the SME loan market, we analyze how SME support financial programs affect the corporate decisions made by SMEs with regard to default or loan sizes. In addition, this paper theoretically computes the optimal levels of credit guarantee amounts and the interest-support spread under equilibrium with imperfect information in a competitive loan market. Second, the paper empirically analyzes the continuous policy-treatment effect with the GPS (generalized propensity score) method. In particular, we consider the ratio of guaranteed debt to the total debt as a continuous policy treatment. The empirical results show that marginal effects of a credit guarantee on SMEs’ productivity, profitability, and growth potential decrease with the ratio of guaranteed debt to the total debt. In addition, the average effect of a credit guarantee is maximized when this ratio is at 50% to 60%.