국제회의 참가기 - I: 나의 ICID 국제집행위원회의 참관기

남우,Nam, U 한국관개배수위원회 2010 한국관개배수회지 Vol.46 No.-

Lactic Acid Bacteria and Natural Product Complex Ameliorates Ovalbumin-Induced Airway Hyperresponsiveness in Mice

남우,김현지,김지수,남보라,배주현,김주연,박수동,이정렬,심재헌 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.5

The incidence of respiratory diseases, such as asthma, has substantially increased in recent times owing to environmental changes, such as air pollution. Induction of a chronic inflammatory response begins with production of biologically active mediators from the airway epithelium, which attracts and recruits inflammatory cells into the lung airway. In our previous study, we confirmed that Lactobacillus casei HY2782 and Bifidobacterium animalis spp. lactis HY8002 could improve lung inflammation in the COPD animal model. In this study, we investigated the effect of the HY2782 complex against airway hyperresponsiveness by using an ovalbumin (OVA)-induced animal model. An orally administered HY2782 complex on OVA-induced allergic asthma in a BALB/c mouse model was used. The present results showed that the HY2782 complex suppressed total immunoglobulin E in serum and bronchoalveolar lavage fluid (BALF). The cytokine production profile in BALF and serum revealed that the HY2782 complex showed reduced levels of Th2 cytokines among immune factors released due to the elevated allergic response. Levels of inflammatory mediators in BALF, MCP-1, MIP-2, and CXCL-9 were decreased by oral administration of the HY2782 complex. Lower numbers of eosinophils and neutrophils in BALF suggested that inflammation was ameliorated by the HY2782 complex. Histological observation of lung sections also showed infiltration of fewer cells. From results, we suggested that the HY2782 complex effectively responds to improvement of the immune response and airway hypersensitivity reaction because of the anti-inflammatory effect of the Pueraria lobata root extract and antioxidant effect of HY2782.

[축사] 특허뉴스 창간 16주년을 축하드립니다

남우기 한국특허신문사 2021 특허 뉴스 : 특허기업의 길라잡이 Vol.- No.181

Lactobacillus paracasei HY7015 Promotes Hair Growth in a Telogenic Mouse Model

남우,김현지,배주현,김지수,남보라,김주연,박수동,이정열,심재헌 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.7

In this study, we describe the effects of Lactobacillus paracasei HY7015 (HY7015) on promoting mouse hair growth. Since our purpose was to increase hair growth through oral administration, medicinal yeast, at a suitable concentration for application in mice, was used as a positive control. First, experiments were conducted to determine the effect of HY7015 on proliferation of hair follicle dermal papilla cells (HFDPC), which are important contributors to hair growth. HY7015 stimulated HFDPC proliferation in vitro and increased their secretion of vascular endothelial growth factor and insulin-like growth factor-1. In mouse experiments, oral administration of HY7015 promoted hair growth and hair follicle maturation in the dorsal skin, as well as increasing growth factor levels in mouse serum. In summary, we demonstrate that L. paracasei HY7015 consumption can promote hair growth by stimulating HFDPC proliferation and growth factor secretion. Follow-up studies are warranted to determine the underlying mechanism, using various approaches, including investigation of changes in intestinal microbiota and alteration of gene and protein expression.

Lactobacillus HY2782 and Bifidobacterium HY8002 Decrease Airway Hyperresponsiveness Induced by Chronic PM2.5 Inhalation in Mice

남우,김현지,배주현,김주연,남보라,이연지,김지수,박수동,이정렬,심재헌 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.6

Epidemiological studies have shown that exposure to particulate matter (PM) is associated with adverse health effects. Inhalation of fine particulate matter (PM2.5) is associated with elevated pulmonary diseases. However, the molecular mechanism underlying the initiation of lung inflammation following inhalation is unclear. In this study, we investigated the beneficial effects of two probiotics, Lactobacillus casei HY2782 and Bifidobacterium lactis HY8002, against PM-induced pulmonary inflammation. Model mice were subjected to chronic exposure of PM2.5. The results showed that PM2.5 enhanced oxidative stress and led to Th2 cytokine responses in the mice. PM2.5-exposed mice were orally administered with HY2782 and HY8002 from the day of first exposure to the end point of the study. The results showed that HY2782 ameliorated PM 2.5 exposure-enhanced leukocyte migration and activation of proinflammatory cytokines. HY2782 and HY8002 also prevented exacerbation of eosinophil and neutrophil infiltration in the bronchoalveolar lavage fluid. HY2782 and HY8002 significantly increased scavenging of PM2.5-induced reactive oxygen species and activated superoxide dismutase and catalase activity in the blood. These results indicate that the probiotics HY2782 and HY8002 protect against PM-induced pulmonary inflammation.

홈네트워크 보안 실상 및 개선방안

남우기 한국정보통신설비학회 2022 한국정보통신설비학회 세미나 Vol.2022 No.03

벼의 Doubled-haploid 집단육성과 SSR 마커를 이용한 유전자 지도작성

김경민,남우일,권용삼,손재근,Kim, Kyung-Min,Nam, Wu-Il,Kwon, Yong-Sham,Sohn, Jae-Keun 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.3

본 연구에서는 1998년부터 2003년 하계까지 약배양 기법 및 콜히친 처리를 이용하여 개발한 '삼강벼/낙동벼' DH(doubled-haploid) 183계통의 주요 농업 형질을 조사$.$분석하였다. DH 집단의 주요 농업형질을 조사한바 초장, 간장, 수장, 삼절간장, 수수 및 출수일수는 양적형질의 특징인 넓은 범위의 변이폭, 연속적인 빈도 분포양상 및 양친을 초월하는 초월분리 현상을 보였다. SSR 마커를 이용한 유전자지도의 작성에는 양친에 다형성을 나타내는 136개의 마커를 사용하였다. 작성된 유전자 지도는 전체 길이가 1,909cM이었으며, 마커간 평균길이는 14 cM을 나타내었다. A doubled-haploid (DH) population was developed through anther culture of F$_1$ plants obtained from a cross between a japonica cultivar, 'Nagdongbyeo', as male parent and a indica cultivar, 'Samgangbyeo', as female parent. Segregation modes for plant length, culm length, panicle length, third internode length, and days to heading in the DH lines showed nearly normal distribution with wide range of variation. A molecular map with 136 simple sequence repeat (SSR) markers was constructed using the DH population. The total map distance was 1,909 cM and the average interval of marker distance was 14 cM.

벼의 Doubled-haploid 집단육성과 SSR 마커를 이용한 유전자 지도작성

김경민,권용삼,남우일,손재근 한국식물생명공학회 2004 JOURNAL OF PLANT BIOTECHNOLOGY Vol.31 No.3

A doubled-haploid (DH) population was developed through anther culture of F1 plants obtained from a cross between a japonica cultivar, Nagdongbyeo', as male parent and a indica cultivar, Samgangbyeo', as female parent. Segregation modes for plant length, culm length, panicle length, third internode length, and days to heading in the DH lines showed nearly normal distribution with wide range of variation. A molecular map with 136 simple sequence repeat (SSR) markers was constructed using the DH population. The total map distance was 1,909 cM and the average interval of marker distance was 14 cM. 본 연구에서는 1998년부터 2003년 하계까지 약배양 기법 및 콜히친 처리를 이용하여 개발한 ‘삼강벼/낙동벼’ DH (doubled-haploid) 183계통의 주요 농업 형질을 조사․분석하였다. DH 집단의 주요 농업형질을 조사한바 초장, 간장, 수장, 삼절간장, 수수 및 출수일수는 양적형질의 특징인 넓은 범위의 변이폭, 연속적인 빈도 분포양상 및 양친을 초월하는 초월분리 현상을 보였다. SSR 마커를 이용한 유전자 지도의 작성에는 양친에 다형성을 나타내는 136개의 마커를 사용하였다. 작성된 유전자 지도는 전체 길이가 1,909 cM이었으며, 마커간 평균길이는 14 cM을 나타내었다.

鹽害土壤改良에 있어서 水理傳導度의 影響

具滋雄,南佑 전북대학교 농업과학기술연구소 1986 農大論文集 Vol.17 No.-

The laboratory study was carried out in order to produce fundamental data for determining water requirements, reclamation periods and reasonable practices for reclaiming salt-affected soils. Reclamation experiments with gypsum treatments were performed to analyze effect of soil hydraulic conductivity on reclaiming salt-affected soils, using 6 soil samples collected in Gyehwa-do reclaimde land Boochang tideland. The results are as follows; 1.The salt-affected soils used in this study were saline-sodic soils with high exchangeable sodium percentage and electrical conductivity. 2.The adsorbed sodium and clay contents of Boochang soils were greater than those of Gyehwa-do soils, and the initial hydraulic conductivity was extremely low in Boochang soils compared with Gyehwa-do soils. 3.The hydraulic conductivity was maintained for a long time with increase of gypsum amount. The effctive amount of gypsum for reclaiming salt-affected soils was more than 1.2 ton/10a in Gyehwa-do soils and 2.4 ton/10a in Boochang soils. 4.When the depth of water leached per unit depth of soil (Dw/Ds) became about 0.3, Gyehwa-do soils were fairly reclaimed and the relative hydraulic conductivity in this case was 73-92% in 1.2 ton/10a gypsum treatment area. 5.Boochang soils were sufficiently reclaimed when Dw/Ds became approximately 0.5, and the relative hydraulic conductivity was about 25% in 2.4 ton/10a gypsum treatment area. 6.The leaching time elapsed until Dw/Ds became 0.3-0.5 in 1.2ton/10a or more gypsum treatment area, was required roughly 10 times in Boochang soils compared with Gyehwa-do soils.

Oral Administration of Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 with Cinnamomi Ramulus Extract Reduces Diet-Induced Obesity and Modulates Gut Microbiota.

정운희,남우,김현지,김주연,남보라,장성식,이정렬,심재헌,박수동 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.2

Obesity is a major health issue worldwide, and is associated with many diseases including type 2 diabetes. In this study, we evaluated the anti-obesity effects of combinations of two lactic acid bacteria (LAB), Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032, and Cinnamomi Ramulus (CR) extract, and explored the mechanism through which they modulate gut microbiota using diet-induced obese mice. Male C57BL/6J mice were randomly divided into five groups that received a high-fat diet (HFD), HFD and LAB (HFD+LAB), HFD and CR extract (HFD+CR), HFD with LAB and CR extract (HFD+LAB+CR), or normal diet for 10 weeks. The mice in the HFD+LAB+CR group showed significant reductions in body weight gain, in particular epididymal fat and liver, blood leptin levels, and an increase in the levels of blood adiponectin. In addition, the LAB and CR extract altered the gut microbiota, mainly increasing the alpha diversity. These results demonstrate that a mixture of two LAB (Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032) and CR extracts alleviate HFD-induced obesity, and has potential of being used as a strategy for the treatment of obesity.