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무궁화꽃을 이용한 L-Arginine Sensor 開發에 關한 硏究
김의락,남기재,최성문,Kim Ui-Rak,Nam Ki-jae,Choi Sung-Moon 대한화학회 1992 대한화학회지 Vol.36 No.5
흰 무궁화꽃의 씨방을 이용하여 Tissue electrode를 제조하고, L-arginine을 정량하기 위한 최적조건을 조사한 결과 0.1M phosphate 완충용액에서 pH 8.0, 온도 $25^{\circ}C$, 조직두께 $20 {\mu}m$이었을 때이며, 이에 정량 가능한 직선범위는 $5.0 {\times} 10^{-3}M $∼ $1.0 {\times} 10^{-1}M$이었으며, 감응도는 54.0 mV/decade로 나타났고, 감응시간은 7∼8분이 소요되었다. $K_m$값은 $6.4 {\times} 10^{-6}(M)$이다. It is shown that receptacle tissue of the rose of sharon can serve as an effective biocatalyst by the construction of a potentiometric membrane electrode with response and selectivity for arginine. Electrodes using this tissue in conjunction with $NH_3$ gas sensing probes yield good response to arginine in $10^{-1}M to 10^{-3}M$ range and retain activity for a least 1 weeks. The arginine-selective membrane electrode using tissue slices of the rose of sharon have good selectivity in presence of other amino acids. The optimum conditions are pH 8.0, $25^{\circ}C$ and $20 {\mu}m$ thickness of slices in 0.1 M phosphate buffer solution and $K_m$ values is $6.4 {\times} 10^{-6}(M)$.
무궁화 꽃을 이용한 Alginine 선택성 sensor 개발에 관한 연구
김의락,손병기,남기재,최성문,전희숙,배진현 경북대학교 센서기술연구소 1991 센서技術學術大會論文集 Vol.2 No.1
It is shown that the rose of sharon receptacle tissue can serve as an effective biocatalyst in the construction of a potentiometric membrane electrode with response and selectivity for arginine, Electrodes using this tissue in conjunction with NH_(3) gas sensing probes yield good response to arginine in 1 X 10^(-1)M to 10^(-3)M range and retain activity for at least 2 weeks. The arginine-selective membrane electrode using tissue slices of the rose of sharon have good selectivity when operated at an optimum pH 8.0, 25℃, 20um thickness of slices and in 0.1M phosphate buffer solution.