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單位生殖에 의한 감자 半數體植物 誘起 Ⅲ. 色素遺傳子 發現樣相에 따른 半數體植物 選拔
Hyun Mook Cho(趙賢默),Hei Young Kim Lee(金惠英),Il Gin Mok(睦一振) 한국육종학회 1993 한국육종학회지 Vol.25 No.1
Interspecific and Interploidal crosses were made between tetraploids and diploids to induce potato haploids. Tetraploids female parents were grouped into three different types according to the pigments on the stem. Pigments on seeds, seedlings and mature plants were used as phenotyphic markers to select haploids. Haploids were derived only from non-embryo spot seeds from all of the three types of the female parents. The positive selection for non-pigmented plants at seed and seedling stage was highly accurate to select haploid in the progenies of the female parents with green stem (group G), except the cv. Russet Burbank. Most of seedlings with white hypocotyl in the progenies of female parents with basal purple(group BP) or whole purple stem(group P), were haploids. However, 45.5% and 69.5% of the seedlings with white-purple hypocotyl derived from these groups were haploids, respectively. Because of the matroclinous effect of their female parents on plant pigmentation, the negative selection for purple pigmented plants at seed, seedling, growth and subsequently, harvest stage, was effective. As a consequence, a two step selection method was applicable for group BP and P ; select the seedlings with only white hypocotyl at seedling stage as haploids, and discard all the plants with purple pigments on the hypocotyl, stem and tuber skin according to gradual plant growth. Up to now, from 1,253 of non-embryo spot seeds, 199 haploid plants were confirmed by the phenotypic markers and subsequently root-tip chromosome counting. The efficiency of haploid induction through interspecific and interploidal crosses was affected by different tetraploids female parents.
감자의 4x×2x 종간교잡 후대 식물체의 배수성 차이에 따른 氣孔細胞의 量的形質 變化와 반수체식물 선발효과
Hyun Mook Cho(趙賢默),Hei Young Kim-Lee(金惠英),Il Gin Mok(睦一振) 한국육종학회 1994 한국육종학회지 Vol.26 No.1
To select haploids from the progeny plants obtained by interploidal and interspecific crosses of potatoes, stomatal cell characters were examined at three different growth stages as a potential indicator of ploidy level. The mean stomatal cell densities were 66.9, 45.0 and 38.5 per unit area(34 ㎛²) in haploid, triploid and tetraploid plants, respectively. The length and width of stomata were 27.5 : 18.8㎛, 34.3 : 21.0㎛ and 36.5 : 22.0㎛ in haploid, triploid and tetraploid plants, respectively. These characters had significant differences between ploidy levels with in the progeny plants but had relatively large quantitative variation within ploidy levels. On the other hand, the number of chloroplasts in a pair of guard cells, having the mean of 12.2, 18.4 and 20.2 in haploid, triploid and tetraploid plants, respectively, had large differences between ploidy levels with narrow variation within a ploidy level. These results indicated that counting chroloplasts in the guard cells among stomatal cell characters could be adopted as a rapid and accurate method for identifying haploids from large number of progeny plants of interploidal and interspecific crosses.
裁培種 감자 半數體와 近緣野生種間 交雜에서 endosperm balance number(EBN)의 영향
조현묵(Hyun Mook Cho),김혜영(Hei Young Kim-Lee),엄영현(Young Hyun Om),김정간(Jung Kan Kim) 한국육종학회 1997 한국육종학회지 Vol.29 No.2
The effect of Endosperm Balance Number (EBN) in interploidal and interspecific crosses between S. tuberosum dihaploids and tuber-bearing wild species was investigated. The crossability was evaluated by the percentage of berry set and the average number of seeds per berry. The percentages of berry set were 11.5%, 11.4%, 7.4% and 2.2% in 4x×4x, 4x×2x, 2x×2x and 2x×4x crosses, respectively. The number of seeds per berry was in the same order as the percentages of berry set. The crossability was greatly affected by the ratio of the maternal and parternal EBN in the endosperm. The crosses with the maternal and parternal EBN ratio of 1 : 1, 1 : 1/2 and 4 : 1 in the developing endosperm did not produce hybrid seeds. However, a lot of plump hybrid seeds were produced only in 2 : 1 of EBN ratio irrespective of differences of the species used or ploidy levels of the parents.
감자의 倍數性 變化에 따른 生育 및 收量關聯形質의 發現
Hyun Mook Cho(趙賢默),Sao Yong Ahn(安手鏞),Hei Young Kim-Lee(金惠英)Hwa Yeong Kim(金和泳) 한국육종학회 1994 한국육종학회지 Vol.26 No.2
The differences in expression of quantitative characters related to top growth and tuber yield of dihaploid(2n=2x=24), triploid(2n=3x=36) and tetraploid(2n=4x=48) potato progenies derived from interspecific crosses were investigated. Plant height and stem diameter among top growth characters and tuber size among tuber characters were significantly reduced according to the downward alteration of the ploidy levels of progeny plants. The terminal leaf index and the weights of tops and tubers of the dihaploid plants were significantly reduced as compared with those of the triploid or tetraploid plants as well, but no significant differences in the numbers of stems and tubers per plant due to the different ploidy levels were shown. However, all quantitative characters related to the top growth and tuber yield of progeny plants affected by ploidy differences showed relatively large variations among not only ploidy levels but also clones in the same ploidy levels.
한국산 마늘 품종 삼척, 대서, 서산의 Giemsa C-banding에 의한 핵형분석
김혜영,이병배 東國大學校 1994 東國論叢 Vol.33 No.-
한국산 마늘 Allium sativum cv 삼척, 대서, 서산에 대하여 Giemsa C-band 법을 통하여 핵형을 조사한바 그 결과는 다음과 같다. 1. 3품종의 염색체수는 모두 2n=16이었다. 2. 체세포분열 중기에서의 염색체의 크기는 품종삼척이 8.1㎛∼15.8㎛ 품종대서는 7.4㎛∼21.0㎛, 품종 서산은 7.4㎛∼19.3㎛이었다. 3. 3품종을 통하여 band의 소재는 염색체 양완의 말단에 주로 위치하고 있었다. 4. 품종 삼척은 F-염색체에, 대서와 서산은 G-염색체에 부수체를 갖고 있었으며 품종 대서와 서산은 H-염색체가 subtelocentric이었으며 나머지는 submetacentric 이었다. Present works were carried out to compare the karyotypes of garlic Allium sativum cv Samcho^k. Daeseo and Seosan. The sizes of somatic chromosomes at mataphase of A. sativum cv Samcho^k, Daeseo, Seosan with 2n=16 were ranged from 8.1㎛ to 15.8㎛, 7.4㎛ to 21.0㎛, and 7.4㎛ to 19.3㎛, respectively. A.sativum cv Daesso, seosan, had one set of subtelocentric chromosomes(chromosome H) with less than 33% or arm ratio. All the others were submetacentric chromosomes. The F chromosomes of A.sativum cv Samcho^k. G chromosomes of Daeseo and Seosan were the sate lite chromosome. Giemsa C-bands of these species are generally located in the end of each arm.