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액체질소 직접분사 냉각방식 mRNA 백신 극저온 저장용기 제작
김기환(Kihwan kim),문정민(Jeongmin Mun),김한영(Hanyoung Kim),김유빈(Yubin Kim),백정현(Jeonghyeon Baek),김석호(Seokho Kim) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.05
In particular, as the mRNA vaccine becomes a major preventive vaccine in the case of COVID-19, the transportation method is being studied to meet global demand. For example, recently developed as a COVID-19 vaccine, Pfizer vaccines should be stored in the temperature range of -70℃, and similar vaccines should also be stored a -30 ℃ or below. However, existing cold storage systems are too high cost to apply storage systems below -30 ℃ and are difficult to apply in transportation. In this study, storage of direct injection using liquid nitrogen was manufactured and maintaining temperature of demanded target. In addition, vaccine storage containers were designed with considering thermal insulation, manufactured, and performance evaluations were implemented.
Sca-1+골수조혈세포에서 JAK2/STAT5/GATA-1 신호전달 경로를 통한 다채, 도두 그리고 두 조합물에 의한 조혈증진 조절에 관한 연구
김근회 ( Kun Hoae Kim ),김승형 ( Seung Hyung Kim ),조인식 ( In Sik Cho ),김한영 ( Han Young Kim ),김동선 ( Dong Seon Kim ),이영철 ( Young Cheol Lee ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.4
Objectives : Brassica campestris var narinosa (BCN), Canavalia gladiata DC semen (CGD) and their combinational prescription (BCN+CGD) have been use to demonstrate to regulate hematopoiesis. In the current study, we investigated whether Brassica campestris var narinosa, Canavalia gladiata DC semen and their combinational prescription is related to hemato-potentiating function using Sca-1+ hematopoietic stem cells (Sca-1+HSCs) as a testing system. Methods : Sca-1+HSCs isolated from femur in C57bl/6 mice with leukopenia and thrombocytopenia induced by cyclophosphamide (CTX). Then, Real-time PCR was performed to measure the mRNA expression, ELISA and haematopoiesis-related gene (EPO, TPO, IL-3, SCF, c-kit, GM-CSF), the phosphorylation of JAK2, GATA-1 and STAT-5a/b were observed by western blot, and the numbers of CD117+/Sca-1+ cell and the number of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E), semisolid clonogenic assay was performed. Result : When Sca-1+HSCs were treated with Brassica campestris var narinosa, Canavalia gladiata DC semen and their combinational prescription with rIL-3/rSCF, the expression of haematopoiesis-related (EPO, TPO, IL-3, SCF, c-kit, and GM-CSF) were significantly increased at the levels of mRNA as well as production in Sca-1+HSCs. Additionally, CGS enhanced phosphorylation of JAK2, GATA-1, and signal transducer and activator of transcription-5a/b (STAT-5a/b) in Sca-1+HSCs. Furthermore, their combinational prescription (BCN+CGD) significantly enhanced the growth rate of granulocyte erythrocyte monocyte macrophage colony-forming units (CFU-GEMM) and erythroid burst forming units (BFU-E) in vitro. Conclusion : These result suggest that Brassica campestris var narinosa (BCN) and Canavalia gladiata DC have hematopoietic enhancement via hematopoietic cytokine-mediated JAK2/GATA-1/ STAT-5a/b pathway, and their combinational prescription (BCN+CGD) has superior hematopoietic enhancement to those of individual extracts.