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항균제 및 향료의 마이크로캡슐을 이용한 항균 · 방향섬유에 관한 연구 (1) - 항균제 / 향료의 마이크로캡슐화 -
김호정,박차철,김한도 ( Ho Jung Kim,Cha Cheol Park,Han Do Kim ) 한국의류학회 1996 한국의류학회지 Vol.20 No.3
Microcapsules containing 2,4,4`-trichloro-2-hydroxydiphenyl ether and perfumes were prepared by the coacervation using poly (vinyl alcohol) and crosslinking agents. Effects of dispersing agents, core materials, agitating speed and crosslinking agents on microcapsule size were investigated. The mean and deviation of microcapsule diameters decreased with increasing agitation speed. The diameters of microcapsules decreased with increasing dispering agent concentration at 6,000 rpm of agitation speed, but it was not changed at 10, 000 rpm. The dispering effect of PVA is better than that of gum arabic. The slight increase in the diameter of microcapsule was observed when the amount of core material was increased. As the amount of crosslinking agent was increased, the diameter of microcapsule was decreased.
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BMP-2를 함유한 2상 알지네이트 담체를 이용한 골수줄기세포의 골분화
임현주,김학태,오은정,김태정,김한도,최진현,정호윤,Lim, Hyun-Ju,Kim, Hak-Tae,Oh, Eun-Jung,Kim, Tae-Jung,Ghim, Han-Do,Choi, Jin-Hyun,Chung, Ho-Yun 대한성형외과학회 2010 Archives of Plastic Surgery Vol.37 No.3
Purpose: The object of this study is to develop a novel BMP-2 delivery system for continuous osteogenic differentiation and to induce osteogenesis of stem cells using a bi-phase alginate carrier in vitro. Methods: Alginate nanoparticle loaded BMP-2 was prepared by the reverse emulsification-diffusion technique. Physical properties and release profiles of alginate carriers were measured by Instron and ELISA kit, respectively. Cell viability and alkaline phosphate activity of hBMSCs differentiation was also evaluated by MTS and Metra BAP assays, respectively. Results: Optimal concentration for bi-phase alginate carrier was determined as 2 wt% by evaluating mechanical and biological properties, and differentiation of BMSCs for bone regeneration. The 2% bi-phase alginate carrier had the lowest initial and final release ratio. In addition, the 2% bi-phase alginate carrier had a little higher ALP activity than the homogeneous carrier. An improved controlled release profile was obtained by combining alginate hydrogel with lyophilized particles. Conclusion: Bi-phase alginate carrier has many advantages such as biocompatibility and controlled release capability. It is expected to be effective as a scaffold and carrier in bone tissue engineering.
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