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        보문 : 생물화공 ; 인공 폐 보조장치 내에서의 유체 유동 모델링에 대한 연구

        김기범 ( Gi Beum Kim ),박영란 ( Young Ran Park ),김상진 ( Shang Jin Kim ),홍철운 ( Chul Un Hong ),강형섭 ( Hyung Sub Kang ),김진상 ( Jin Shang Kin ),김성종 ( Seong Jong Kim ),김민호 ( Min Ho Kim ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.2

        본 연구에서는 혈관 내 인공 폐(intravenous lung assist device)를 개발하기 위하여 CFD를 사용하여 새로운 형태의 인공 폐 모형에 대한 유체의 흐름 특성을 모델링하였다. 모델링을 위하여 중공사(hollow fiber)는 무시하였으며 vertical type과 tangential type이 모델로 사용되었다. 유체의 흐름 특성을 예측하기 유체의 입출구로 1개로 하였을 때와 2개로 하였을 때 그리고 입출구를 관의 중심부(vertical)와 관 벽의 접선방향(tangential)에 위치하였을 때의 흐름 특성을 파악하였다. 실험 결과, tangential type과 같이 원통의 접선 방향으로 유체의 입구와 출구를 설정할 경우 vertical type에서 나타나는 흐름이 없는 영역(정체층)을 제거할 수 있었다. 또한 tangential type은 와류형태의 흐름이 지배적이며 한쪽으로 편중된 흐름이 아닌 복잡한 형태의 흐름이 발생하는 것으로 나타났다. 또한 유체의 입출구가 2개일 때 유체가 편중된 흐름이 발생하지 않고 관 전체에 복잡한 형태로 흐름이 발생하는 것으로 나타났다. 실험 결과를 통하여 우리는 유체가 유입되는 입구와 출구가 tangential type이며 각각 2개일 때 유체의 흐름이 복잡하며 정체층이 발생하지 않는 흐름이 발생한다는 것을 확인할 수 있었다. In this study, the characteristic of fluid flow in the new type lung assist devices has been established using computational fluid dynamics(CFD). For the modeling, the hollow fiber was ignored, and vertical types and tangential types were used for the model. Which was to analyze the flow characteristics of the fluid Dow model when there exists 1 and 2 input/output pods, and when the input/output ports is located at the center of the cylinder and at the tangential direction with the cylinder wall. The modeling results showed that it was possible to eliminate no-flow region(stagnant layer) as shown in the vertical type when an inlet and an outlet were installed on the tangential direction of the cylinder as shown in the tangential type. Also, in the tangential type, vortex-type flow appeared as dominant, and it showed a complicated flow not deviated to one side. When the number of input/output was two, there was no deviated flow, and complicated flows were generated all across the tube. From the test result, it was found that input/output of flow was tangential type and complicated flows with no stagnant layer would be generated when there are two inputs/outputs, respectively.

      • KCI등재

        보문 : 생물화공 ; 자가면역글로불린 G 측정을 위한 표면탄성파 바이오센서에 대한 연구

        김기범 ( Gi Beum Kim ),정우석 ( Woo Suk Cheong ),박영란 ( Young Ran Park ),김상진 ( Shang Jin Kim ),김성종 ( Seong Jong Kim ),강형섭 ( Hyung Suk Kang ),김진상 ( Jin Shang Kim ),홍철운 ( Chul Un Hong ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.2

        본 연구에서 탄성표면파(SH-SAW) 디바이스의 지연선에 코팅된 골드 위에서 면역 글로불린 G(IgG) 검출을 위한 SH-SAW 센서를 개발하고자 하였다. 실험결과, 금표면 위에 anti-MIgG 혼합물을 일관되게 고정시킬 수 있었다. G-anti MIgG 혼합물과 blocking buffer를 이용한 골드 표면 위에 고정화 하였을 때 주파수 변위를 측정한 결과, G-anti MIgG는 초기 주파수에서 75.1 kHz 주파수 변위를 보였으며 blocking buffer는 215.7 kHz의 주파수 변위를 보였다. 100 MHz 센서에서 MIgG의 농도가 25, 50, 75, 100 μg일 때 46.3, 127.45, 161.21, 262.39 kHz 주파수 변위를 보였다. In this study, we have developed shear horizontal(SH) surface acoustic wave(SAW) sensors for the detection of immunoglobulin G(IgG) on the gold coated delay line of SH-SAW devices. As the result of the experiment, we could uniformly immobilize anti-MIgG(mouse IgG) conjugate on the surface of gold. When displaying results of immobilization on the surface of gold using G-anti MIgG conjugate and blocking buffer in frequency shift, G-anti MIgG conjugate showed frequency shift of 75.1 kHz in the initial frequency, and blocking buffer showed frequency shift of 215.7 kHz. When various concentrations of MIgG was added in 100 MHz type sensor, the sensor showed 46.3, 127.45, 161.21 and 262.39 kHz frequency shift at 25, 50, 75 and 100 μg MIgG concentration, respectively.

      • KCI등재후보

        Microencapsulation 헤모글로빈 인공적혈구의 용혈

        김민호 ( Min Ho Kim ),강형섭 ( Hyung Sub Kang ),김진상 ( Jin Shang Kim ),백병걸 ( Byeong Kirl Baek ),홍철운 ( Chul Un Hong ),김성종 ( Seong Jong Kim ),신형식 ( Hyung Shik Shin ),김기범 ( Gi Beum Kim ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.2

        The purpose of this study was to reduce the hemolysis of artificial red blood cells by extracting phospolipids from egg yolk through microencapsulation to evaluate the oxygen transfer rate of artificial red blood cells in an intravascular artificial lung device. In this study, hemosome was produced to encapsulate hemoglobin which extracted the red blood cell of bovine blood with phospolipids reduced egg yolk. The solution blended blood with hemosome at the rate of 4 to 1 and normal blood measured the oxygen transfer rate. The hemolysis of the microencapsulated artificial red blood cell measured less than the normal red blood cell through absorptivity. Due to the phospolipids enclosing the red blood cell to protect it, the produced hemosome operated at a higher oxygen transfer rate by reducing the demolition of hemoglobin. Consequently, hemolysis was observed to advance through micoencapsulation.

      • KCI등재
      • KCI등재

        보문 : 생물화공 ; CFD 해석을 이용한 Balloon형 인공심폐기 설계를 위한 구조적 해석

        박영란 ( Young Ran Park ),심정연 ( Jeong Yeon Shim ),김기범 ( Gi Beum Kim ),김상진 ( Shang Jin Kim ),강형섭 ( Hyung Sub Kang ),김진상 ( Jin Shang Kim ),김민호 ( Min Ho Kim ),홍철운 ( Chul Un Hong ),김성종 ( Seong Jong Kim ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.2

        본 연구는 기존의 인공심폐기의 단점을 보완하기 위하여 혈액펌프를 사용하지 않고 혈류의 흐름을 유도할 수 있는 기구(balloon)형 인공심폐기 설계를 위한 구조적 해석을 시도하였다. 가상의 모형 인공심폐기 내에서의 혈류의 흐름패턴을 분석하기 위하여 CFD 모델링 방법을 사용하였다. 이 시스템의 작동원리는 막 산화기 주위를 기구를 사용하여 압력하중을 인가하여 주기적으로 수축·이완되도록 하였으며, 시간에 따라 변화하는 시간 함수 값은 sine 반주기와 sine 주기를 계산하여 적용하였다. 이와 같은 방법으로 기구형 인공심폐기를 설계할 경우 한 방향에 대한 혈류의 움직임을 유도할 수 있다는 가정 하에 구조적 해석을 하였다. 실험결과 CFD 시뮬레이션을 통하여 인공심폐기의 입구와 출구에서의 혈류의 속도와 압력을 측정하여 분석한 결과 한 방향에 대한 혈류의 유동이 발생하는 것을 확인하였다. 이와 같은 CFD 시뮬레이션은 혈류의 흐름특성을 미리 예측할 수 있어 인공심폐기 설계에 있어서 최적화된 디자인을 제공할 수 있을 것이라 판단된다. In this study, we attempted a structural analysis in order to design a balloon type extracorporeal membrane oxygenator that can induce blood flow without using blood pumps for the purpose of complementing the weakness in the existing extracorporeal membrane oxygenator. To analyze the flow characteristic of the blood flow within the virtual model of extracorporeal membrane oxygenator, computational fluid dynamics(CFD) modeling method was used. The operating principle of this system is to make the surface of the extracorporeal membrane oxygenator keep contracting and dilating regularly by applying pressure load using a balloon, and the ``Time Function Value`` that changes according to the time was applied by calculating a half cycle of sine waveform and a cycle of sine. waveform Under the assumption that the uni-directional blood flow could be induced if the balloon type extracorporeal membrane oxygenator was designed as per the method described above, we conducted a structural analysis accordingly. We measured and analyzed the velocity and pressure of blood flow at both inlet and outlet of the extracorporeal membrane oxygenator through CFD simulation. As a result of the modeling, it was confirmed that there was a flow in accord with the direction of the blood by the contraction/dilation. With CFD simulation, the characteristics of blood flow can be predicted in advance, so it is judged that this will be able to provide the most optimized design in producing an extracorporeal membrane oxygenator.

      • KCI등재

        환우(換羽, molting)에 의한 절식 후 산란계의 혈액 Mg2+과 K+ 변동

        고현규 ( Hyeon Kyu Go ),이세진 ( Sei Jin Lee ),조인국 ( In Gook Cho ),이문영 ( Mun Young Lee ),박혜민 ( Hye Min Park ),문아름 ( A Reum Mun ),김정곤 ( Jeong Gon Kim ),김기범 ( Gi Beum Kim ),김진상 ( Jin Shang Kim ),강형섭 ( Hyung S 한국임상수의학회 2011 한국임상수의학회지 Vol.28 No.6

        자연적 혹은 인위적 환우 기간의 절식은 산란계에서 심각한 대사성 스트레스가 될 수 있다. 절식 그리고 필수적으로 수반되는 사료 재급여 증후군의 대사성 스트레스는 ATP 생성과 밀접한 Mg2+, K+과 P 등의 무기염류 불균형을 야기할 수 있다. Mg2+은 생체 대상과정에서 필수적인 무기염류이며 스트레스는 생체 Mg2+ 요구량을 증가시킬 뿐만 아니라 Mg2+ 결핍을 야기할 수 있기 때문에 산란계에서 환우 기간의 절식에 관련된 혈액내 이온의 이온화 농도 및 결합형을 포함한 총농도의 변동을 관찰하였다. 환우 후에 대사성 스트레스와 관련된 생화학 인자의 변화와 수반하여 혈액내 Mg2+과 K+의 감소가 관찰되었다. 따라서 환우 기간의 절식 및 사료 재급여 증후군은 심각한 저마그네슘혈증 및 저칼륨혈증을 야기할 수 있으므로 환우 그리고 재급여 과정에서 Mg2+과 K+의 투여가 권장된다. Either the fasting during natural molting or the starvation in induced molting would be a severe metabolic stress to laying hens. The metabolic stress during starvation and subsequent refeeding syndrome could lead to unbalance of mineral homeostasis, including Mg2+, K+ and P required by ATP synthesis. Since Mg2+ is a fundamental ion for normal metabolic processes and stress may not only increase in demands of Mg2+ but also produce consequence of Mg2+ deficiency, we investigated the changes of blood ionized and total ions related to starvation during molting in laying hens. We founded the significant decrease in blood Mg2+ and K+ accompanied by the changes of biochemical parameters relating to increased metabolic stress after molting. These results suggested that appropriate Mg2+ and K+ supplements to laying hens could have beneficial effects during molting and subsequent refeeding that could produce a severe hypomagnesemia and hypokalcemia.

      • KCI등재

        육계에서 sulfathiazole 경구투여 후 혈장 및 조직내 잔류량

        서형석 ( Heyng Seok Seo ),임정철 ( Jung Chul Lim ),허부홍 ( Boo Hong Hur ),권정택 ( Jung Taek Kwon ),김성문 ( Seong Moon Kim ),천희웅 ( Hee Woong Chun ),최인방 ( In Bang Choi ),김진상 ( Jin Shang Kim ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3

        The purposes of this study were to characterize the disposition of sulfathiazole(ST) and to investigate the effects of sodium bicarbonate on the disposition of ST in broiler chicks(2.5~3.0kg). Animals were given ST acutely(10~80mg/kg, PO), and plasma, kidney, muscle, heart, liver and spleen samples were collected and analyzed for ST by high performance liquid chromatography. The plasma and tissue data was consistent with a one-compartment pharmacokinetic model. The drug is rapidly but incompletely(2.5~3.87%) absorbed with peak plasma and tissue levels being achieved within one hour after dosing. The plasma and tissue levels depended on drug dosage, and the descending order in concentration of ST was kidney>plasma>heart>muscle≥spleen≥liver from animals sacrificed at one hour after dosing. Moreover, significant positive correlations(r>0.9) existed between plasma and tissue levels of ST. In addition, sodium bicarbonate pretreatment decreased plasma level, indicating that an alkalinization stimulate the excretion of ST. Results of this study suggest that oral application of ST was rapidly absorbed and eliminated, and confirmed that tissue residues of ST can be estimated from plasma drug concentration in broiler chicks.

      • KCI등재

        기니픽에서 Magnesium의 혈관 이완과 혈압 하강 효과

        김정곤,강형섭,김진상 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2

        The present study evaluated the effects of various agents on magnesium sulfate (Mg^(2+))-induced relaxation in aorta and blood pressure lowering in anesthetized guinea pigs. Mg^(2+) inhibited phenylephrine (PE)- or 40 mM KC1-induced sustained contraction of endothelium-intact (+E) guinea pig aortic rings in concentration-dependent manner. In preparations preconstricted with PE or KC1, Mg^(2+)-induced concentration-dependent relaxations. In preparations preconstricted with PE or KCl, Mg^(2+)-induced relaxations were not affected by the removal of endothelium (-E) and by the pretreatment of aortic rings with NO synthase (NOS) inhibitors (L-NAME and LNNA), guanylate cyclase inhibitors (methylene blue and ODQ, adenylate cyclase inhibitor (MDL), K^(+) channel blockers (glibenclamide and tetrabutylammonium), nifedipine or ryanodine. However, Mg^(2+)-induced relaxations were inhibited by Na^(+)-Mg^(2+) exchange inhibitor (imipramine) or removal (or attenuation) of extracellular Ca^(2+) in +E aortic rings. PE-induced contraction was not inhibited by nifedipine. In addition, Mg^(2+)-induced relaxations were inhibited by phospholipase C inhibitor (NCDC) or inositol monophosphatase inhibitor (lithium), but not by protein kinase C inhibitor (staurosporine). In vi패 infusion of Mg^(2+), directly into the femoral veins of guinea pigs, elicited sustained decrease in arterial blood pressure. The Mg^(2+)-lowered blood pressure was attenuated by intravenous administration of imipramine or lithium, but not by methylene blue, indomethacin, tetrabutylammonium nifedipine, LNNA, L-NAME or saponin (as an endothelium removal agent). These results suggest that endothelium independent vasorelaxant effect of Mg^(2+) on aortic ring appear to play important roles on the antihypertensive action in guinea pig, most likely via the inhibitory action of Mg^(2+) on the intracellular C^(2+) involve in PLC-IP pathway and influx (through the Na^(+)-Mg^(2+) exchanger) into the cell in guinea pig aorta.

      • KCI등재

        관류 심장과 심장 세포에서 Insulin에 의한 Mg^(2+) 유리 조절

        정창우,강형섭,김진상 한국수의공중보건학회 2002 예방수의학회지 Vol.26 No.3

        It is well known that β-adrenergic stimulation increases the intracelluar cAMP concentration through activation of adenylate cyclase (AC) and evokes marked Mg^(2+) release in the heart, liver, and kidney. The goal of this study was to investigate the effect of insulin on isoproterenol (ISO), norepinephrine (NE), forskolin (FOS), cAMP, or dimaprit (DMP) induced Mg^(2+) efflux from the perfused rat or guinea pig heart and isolated myocytes. We hypothesized that insulin would regulate Mg^(2+) efflux induced by AC activators and cAMP analogues because insulin activates phosphodiesterase (PDE) in the hearts. The Mg^(2+) content in the perfusate was significantly higher in the presence than in the absence of insulin. The addition of ISO, NE, FOS, or cAMP to perfused rat or guinea pig heart and isolated myocytes induced a marked Mg^(2+) efflux. These effluxes were inhibited by insulin. The Mg^(2+) efflux could also be induced by DMP, a histamine H2-receptor agonist, in the perfused guinea pig heart and isolatd myocytes. This effect was also inhibited by insulin. In rat heart and myocytes, the histamine H2-receptor agonist had no effect on Mg^(2+) efflux. In conclusion, these data suggest that insulin regulates Mg^(2+) homeostasis and the inhibitory effect of insulin on adrenoceptor-stimulation or AC activation induced Mg^(2+) efflux may occur through a regulation of cAMP pathway in rat and guinea pig hearts.

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