삼잎국화 추출물의 피부세포 생리활성 효과

김준섭,Kim, Jun-Sub 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.3

The objective of the present investigation was to obtain vitamin, mineral, flavonoid, and polyphenol profiles of Rudbeckia laciniata (RL), and to examine the effects of extract of RL (RLE) on various physiological activities of HaCaT keratinocyte for the utilization of RL as natural raw materials to develop functional food. To accomplish this purpose, we checked the contents of the general nutrients of RL. The contents of vitamin A, vitamin $B_1$ and vitamin $B_2$ were $7.49{\mu}g/g$, $51.96{\mu}g/g$, and $132{\mu}g/g$ respectively, while vitamin C and vitamin $D_3$ were not detected. The contents of mineral such as Ca, K and Fe were 2.01 mg/g, 6.06 mg/g and 0.03 mg/g respectively. Total flavonoid contents of RLE were 0.25 mg/g, and total polyphenol were estimated as 1.43 mg/g. Because RL contains high levels of vitamin A which is associated with skin aging, we investigated the effect of RLE on physiological function of keratinocytes with respect to skin aging. We found that RLE significantly increased the growth rate of HaCaT cells and reduced ultraviolet radiation B (UVB)-induced cellular toxicity. Also, the extract of Rudbeckia laciniata attenuated the UVB-induced reactive oxygen species (ROS) generation in a dose-dependent manner in HaCaT cells. In addition, treatment with the extract dose-dependently increased migration activity of HaCaT cells. Thus, these findings indicated that RLE could regulate the physiological activity of keratinocytes, and may be used to develop functional foods.

역마이셀을 이용한 Sm₂O₃ 도핑 CeO₂ 나노분말의 합성 및 특성

김준섭,배동식,Kim, Jun-Seop,Bae, Dong-Sik 한국재료학회 2012 한국재료학회지 Vol.22 No.4

The preparation of $Sm_2O_3$ doped $CeO_2$ in Igepal CO-520/cyclohexane reverse micelle solutions has been studied. In the present work, we synthesized nanosized $Sm_2O_3$ doped $CeO_2$ powders by reverse micelle process using aqueous ammonia as the precipitant; hydroxide precursor was obtained from nitrate solutions dispersed in the nanosized aqueous domains of a micro emulsion consisting of cyclohexane as the oil phase, and poly (xoyethylene) nonylphenylether (Igepal CO-520) as the non-ionic surfactant. The synthesized and calcined powders were characterized by Thermogravimetry-differential thermal analysis (TGA-DTA), X-ray diffraction analysis (XRD), and Transmission electron microscopy (TEM). The crystallite size was found to increase with increase in water to surfactant (R) molar ratio. Average particle size and distribution of the synthesized $Sm_2O_3$ doped $CeO_2$ were below 10 nm and narrow, respectively. TG-DTA analysis shows that phase of $Sm_2O_3$ doped $CeO_2$ nanoparticles changed from monoclinic to tetragonal at approximately $560^{\circ}C$. The phase of the synthesized $Sm_2O_3$ doped $CeO_2$ with heating to $600^{\circ}C$ for 30 min was tetragonal $CeO_2$. This study revealed that the particle formation process in reverse micelles is based on a two step model. The rapid first step is the complete reduction of the metal to the zero valence state. The second step is growth, via reagent exchanges between micelles through the inter-micellar exchange.

빈카 마이너 추출물의 항균 및 면역활성 연구

김준섭,강조은,유일환,정경환,문기성,이향렬,Kim, Jun-Sub,Kang, Jo-Eun,Yu, Il-Hwan,Jung, Kyung-Hwan,Moon, Gi-Seong,Lee, Hyang-Yeol 한국응용과학기술학회 2015 한국응용과학기술학회지 Vol.32 No.1

EtOH 추출법과 열수 추출법을 사용하여 빈카민 등의 빈카 알칼로이드 화합물을 함유한 빈카 마이너 추출물을 얻었다. 이들 추출물을 사용하여 비듬균, 효모, 그람양성 및 음성세균에 대한 항균활성을 측정 하였다. 비듬균(Malassezia furfur)에 대하여 표준물질인 빈카민과 EtOH 추출물은 항균활성을 보이지 않은 반면 열수 추출물은 항균활성을 나타내었다. 또한 열수 추출물은 그람양성 및 음성세균에 속하는 바실러스(Bacillus sp.)와 대장균(Escherichia coli)에 대해서도 항균활성을 나타내었다. 추출물의 세포독성은 HaCaT 세포(인간 케라티노사이트 세포), HT-29 세포(인간 결직장 선종암 세포), Raw 세포(인간 대식세포)를 대상으로 측정하였으며, 해당 세포들에 대해 특별한 세포독성은 발견 되지 않았다. 또한 형광기반의 탐색물질인 디클로로플루오신 디아세테이트(DCFDA)를 이용한 활성산소종을 측정하였다. 그 결과 HaCaT 세포와 HT-29 세포군에서 활성산소종의 형성이 약 20% 정도 증가하는 것을 알 수 있었다. Vinca alkaloid compounds including vincamine from Vinca minor L. have been extracted by ethanol and hot water extraction methods. Antimicrobial properties of those extracts have been investigated against dandruff causing microorganism Malassezia furfur, yeast, Gram positive and negative bacteria. Vincamine standard and ethanol extract showed no sign of antimicrobial activity, whereas hot-water extract had the activity against M. furfur. Furthermore hot water extract had antimicrobial activity against Gram positive Bacillus sp. and Gram negative Escherichia coli. Cytotoxic properties of those extracts have also been investigated with HaCaT cell (human keratinocyte), HT-29 cell (human colorectal adenocarcinoma cell) and Raw cell, showing no significant cytotoxic effects. We also measured the ROS using dichlorofluorescein diacetate (DCFDA), a popular fluorescence-based probe for reactive oxygen species detection. The result showed the increasement of reactive oxygen species formation (20%) in HaCaT and HT-29 cell lines.

빈카 마이너 추출물에 함유된 알칼로이드들의 분광학적 분석 및 항산화 효능 연구

김준섭,주지혜,강조은,장선동,정경환,문기성,이향렬,Kim, Jun-Sub,Joo, Ji-Hye,Kang, Jo-Eun,Jang, Sun-Dong,Jung, Kyung-Hwan,Moon, Gi-Seong,Lee, Hyang-Yeol 한국응용과학기술학회 2015 한국응용과학기술학회지 Vol.32 No.1

빈카마이너 L.의 알칼로이드는 HPLC의 PDA 검출기를 이용하여 측정하고 이를 표준시료인 빈카민의 자외선/가시광선 흡수 스펙트럼과 비교하여 분석하였다. 플라보노이드류를 포함하여 추출물에서 9개 이상의 화합물에 대한 자외선/가시광선 흡수 스펙트럼을 얻었다. 추출방법에 따라 에탄올 추출물과 열수 추출물을 얻고 이를 DPPH 라디칼 소거법에 의해 항산화 효과를 조사하였다. 그 결과 에탄올 추출물과 열수 추출물 모두 비교군인 케르세틴과 비타민 C 대비 약 14~15%의 항산화 효과를 보여 추출법에 상관없이 유사한 결과를 보였다. Alkaloids of Vinca minor L. were characterized by HPLC equipped with the photo diode array detector and compared their UV/vis absorption spectra with an index material such as vincamine. UV/vis absorption spectra of more than 9 compounds belong to various classes such as flavonoids were shown and characterized. Extracts with ethanol or hot water were tested against the DPPH (2,2-diphenyl-1-picrylhydrazyl) free-radical to investigate their antioxidant activity. Based on the results, those extracts show about 14-15% of antioxidant activity of quercetine and vitamin C used as standards.

대식세포의 혈청으로 식균된 자이모잔의 탐식능에 대한 삼잎국화 추출물의 효과

김준섭,Kim, Jun-Sub 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.3

Phagocytosis is a primary and an essential step of host defense, and is triggered by the interaction of particles with specific receptor of macrophages. In this study, we investigated the effect of extracts of Rudbeckia laciniata (RLE) on the phagocytic activity of macrophage, by monitoring the phagocytosis-associated signal transduction. RLE markedly increased phagocytosis of serum-opsonized zymosan particles (SOZ), while phagocytosis of IgG-opsonized zymosan particles (IOZ) or none-opsonized zymosan particles (NOZ) remained unaffected. However, RLE did not affect the binding of opsonized zymosan particles (OZ) with the cell surface of macrophage. This suggests that RLE may regulate SOZ-induced intracellular signaling during phagocytosis of macrophage. To confirm this hypothesis, we investigated whether RLE was involved in the RhoA-mediated signal transduction during phagocytosis of SOZ. Inhibitors of the RhoA-mediated signaling pathway, such as Y-27632 (for ROCK), ML-7 (for MLCK), and Tat-C3 (for RhoA), totally blocked phagocytosis of SOZ enhanced by RLE, as well as phagocytosis of SOZ. Additionally, RhoA activity was markedly increased when cells were treated with RLE, suggesting that RLE could increase the phagocytic activity of macrophage via RhoA-ROCK/MLCK signal pathway. Thus, RLE may be used to develop functional foods for immunity.

경남 통영 민양마을 잘피밭의 어류 종조성

김준섭,최정윤,이유진,곽우석,Kim, Jun-Sop,Choi, Jung-Yoon,Lee, Yu-Jin,Gwak, Woo-Seok 한국해양학회 2013 바다 Vol.18 No.4

경남 통영시 민양 잘피밭에 서식하는 어류의 종조성 및 계절 변동을 조사하기 위해 2011년 5월부터 2012년 4월까지 예인망(surf net)을 이용하여 매월 어류를 채집하였다. 조사기간 동안 총 25종 3,880개체 3,145.1 g의 어류가 채집되었다. 조사기간 동안 채집된 어류는 대부분 소형어나 유어들이었고, 그 중 우점종은 1,970개체가 채집된 살망둑(Gymnogobius heptacanthus)이였으며, 그물코쥐치(Rudarius ercodes), 베도라치(Pholis nebulosa), 별망둑(Chaenogobius gulosus), 날망둑(Gymnogobius castaneus) 순으로 채집되어 총 개체수의 89.1%를 차지하였다. 잘피밭 출현 어류는 수온이 대체적으로 높은 6-9월 사이 다수의 개체가 출현하였고, 수온이 낮은 1-2월에 개체수와 종수가 적게 나타나 수온의 영향을 받고 있는 것으로 보인다. 한편, 종 다양도지수(H')는 2012년 4월이 가장 높게 나타났다. A total of 25 species, 3,880 individuals and 3,145.1 g of fishes were collected by a surf net from the eelgrass bed in Minyang. Most fishes collected in the eelgrass bed belong mainly to small fish species or juveniles. The dominant fish species were Gymnogobius heptacanthus, Rudarius ercodes, Pholis nebulosa, Chaenogobius gulosus, and Gymnogobius castaneus which accounted for 89.1% of total number of individuals collected. The number of individuals and biomass were higher from June to September when the water temperature was high. However, both the number of individuals and species showed the lowest from January and February. Highest species diversity index (H') was observed in April, 2012.

Hydroxymethylstyrene 아이오노머의 클러스터상 형성

김준섭,김선일,Kim, Joon Seop,Kim, Sun Il 대한화학회 1998 대한화학회지 Vol.42 No.6

해양플랜트용 LNG 제어용 초고압/초저온 밸브 개발

김준섭(J.S. Kim),김규철(G.C. Kim),이재익(J.Y. Lee),김기혁(G.H. Kim),장성철,김영주(Y.G. Kim),우남섭 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12

Numerical simulation using CFD(Computational Fluid Dynamic) in order to predict a mass flow rate and a flow coefficient form flow dynamic point of view. The outlet pressure was fixed by atmospheric pressure and calculated until increasing 1bar to 10bar. CFD analysis used STAR-CCM+ which is commercial code and Governing equations were calculated by moving mesh which is rotated 90 degrees when ball valve operated opening and closing in 1 degree interval. The result shows change of mass flow rate according to opening and closing angle of valve. The structural analysis used ANSYS which is a commercial code. Stress analysis result of internal pressure in valve showed lower than yield strength. This is expect to need more detail design and verification for stem and seat structure.

해양플랜트용 고압/고차압 제어밸브의 트림 개발

김준섭(J.S. Kim),김규철(K.C. Kim),김기혁(G.H. Kim),장성철(S.C. Jang),우남섭(N.S. Woo),김영주(Y.J. Kim) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12

This study have goal with conceptual design for offshore structures of high pressure drop control valve for localization valve for development accomplished with flow analysis based on provision of ANSI B16.34, ANSI B16.10, ANSI B16.25 In order to localize the Offshore structures high pressure drop control valve. This study is numerical analysis for zambil offshore project of high pressure drop control valve. The solver which ANSYS workbench used for offshore structures analysis. The working fluids assumed the glycerin(C3H8O3). The structural analysis used ANSYS which is a commercial code. Stress analysis result of internal pressure in valve showed lower than yield strength. This is expect to need more detail design and verification for stem and disk structure.

시화조력 발전기와 수력발전기의 동특성 비교분석

김희곤(Kim Hee Gon),장태현(Jang Tae Hyeon),김지찬(Kim Ji Chan),김종득(Kim Jong Deug),김준섭(Kim Jun Sub),옥연호(Ok Yeon Ho) 대한전기학회 2015 대한전기학회 학술대회 논문집 Vol.2015 No.7