GFP 리포터를 이용한 외부 푸마르산 유도 dctA 유전자 발현 특성 파악

김주한,홍순호,Kim, Joo-Han,Hong, Soon-Ho 한국미생물학회 2011 미생물학회지 Vol.47 No.2

본 연구에서는 외부의 푸마르산을 인식하는 DcuS/R TCS에 의하여 발현이 조절되는 dctA 유전자의 발현 특성을 GFP를 이용하여 관찰하였으며, 1 mM의 푸마르산을 감지하여 GFP를 발현 시킬 수 있음을 확인하였다. 결론적으로, 개량된 E. coli는 간단한 dctA 프로모터와 GFP의 융합을 이용하여 푸마르산을 모니터 할 수 있으며, 이것은 상승된 푸마르산 농도 조건하에서 원활히 작동함을 확인할 수 있었다. In Escherichia coli, DcuS/R two-component system controls fumarate import and utilization related gene expression. To understand the dynamic response of the bacterium DcuS/R two-component system with respect to fumarate concentrations, DcuS/R induced dctA promoter was integrated with GFP reporter protein. Expression monitoring study using recombinant strain showed that dctA promoter was upregulated with 1 mM of fumarate in M9 minimal medium.

정상 골조직과 경계가 불명확한 요추 골연골종 - 증례보고 -

김주한,오우석,정흥섭,이기찬,서중근,Kim, Joo-Han,Oh, Woo-Suk,Chung, Hung-Seob,Lee, Ki-Chan,Suh, Jung-Keun 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.6

Spinal osteochondroms are very rare, and are thought to arise through a process of progressive endochondral ossification of aberrant cartilage of a growth plate, as a consequence of congenital defect or trauma. A case of diffuse type osteochondroma involving the posterior elements of L1-L5 that progressed after laminectomy in a 33-year-old man is reported. Usually, the spinal osteochondroma shows clear demarcation between tumor margin and normal spine elements, and can be exised completely. However, there was no clear demarcation between tumor and normal spine element in our case and therefore it was not possible to removal completely.

Desmoplastic Fibroma of the Skull;A Case Report, Review of the Literature, and Therapeutic Implications

김주한,박정율,정용구,서중근,김성남,서연림,Kim, Joo Han,Park, Jung Yul,Jung, Yong Gu,Suh, Jung Keun,Kim, Sung Nam,Suh, Yeon Lim The Korean Neurosurgical Society 2001 Journal of Korean neurosurgical society Vol.30 No.8

결합조직형성 섬유종은 드문 골종양으로 조직학적으로는 양성이나 국소적으로 빨리 자라는 질환이다. 두개골에 발생한 결합조직형성 섬유종은 지금까지 9례가 보고되고 있으며, 이 중 8명은 여자에서 발생하다. 이 논문은 남자에서 발생한 두개골 결합조직형성 섬유종을 보고하며, 문헌을 검토하여, 이 질환의 임상양상 및 조직학적소견, 그리고 치료방법등을 보고하고자 한다. 환자는 21세 남자로 두통을 호소하으며, 두부 전산화 단층 촬상 뇌을 침습한 소견이 없는 단일 골용해성 부위가 관찰되었으며, 조직학적 검사상 결합조직형성 섬유종으로 밝혀졌다. 면역조직화학적 염색상, 종양의 에스트로겐과 프로게스테론 수용기는 음성이었다. 정상골을 포함한 종양을 전적출하으며, 35개월간 추적관찰결과 재발소견은 없었다. 다른 부위의 결합조직형성 섬유종과 비교했을 때, 종양의 정상골을 포함한 전적출이 두개골에서 가능하므로, 재발이 없는 것으로 생각되며, 남자에서 발생한 결합조직형성 섬유종을 치험하기에 문헌검토와 함께 보고하는 바이다. Desmoplastic fibroma(DF) is a rare neoplasm of the bone, and is histologically benign but locally aggressive disease. A total of nine cases of DF involving skull have been reported in the literature, and among these eight are females. In this report, the clinical findings and histopathology of a case with DF of the skull in a male patient is presented with a review of the literature with an emphasis on treatment modalities. A 21-year-old man presented with headache. CT scan revealed a solitary and lytic skull lesion without brain invasion. DF was confirmed by histological evaluation. On immunohistochemical staining of the tumor was negative for estrogen or progesterone receptors. After total resection of tumor with wide surgical margin, there was no recurrence during the 35 months of follow-up period. Although longer follow up period maybe needed, treatment of this type of tumor with complete resection of tumor tissue along with a wide margin may provide long disease-free state compare to the high recurrence rates in DF of other sites.

신경교종에서 뇌동맥내 Carboplatin주입후 발생한 안구 합병증 - 3례보고 -

김주한,이장보,정용구,박정율,이훈갑,서중근,Kim, Joo-Han,Lee, Jang-Bo,Chung, Yong-Gu,Park, Jung-Yul,Lee, Hun-Kap,Suh, Jung-Keun 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.5

Carboplatin intra-arterial chemotherapy(IAC) has an advantage of increased uptake during the first passage of the drugs through tumor capillaries. Although not common, this type of therapy is known to cause neurological complications, myelosuppression, and ototoxicity. However, the incidence of ocular toxicity is reported to be rare. Eleven of our patients with glioma(Grade II Astrocytoma : 3, Grade III Astrocytoma : 1, Grade IV Astrocytoma : 5, Gliofibroma : 1, Oligodendroglioma : 1) underwent IAC regimen with carboplatin($300mg/m^2$) which were administrated after blood-brain barrier disruption. Of there, 3 patients had ocular complications after supra-ophthalmic IAC injection of carboplatin but fully recovered following steroid therapy. Although our results from IAC seem to be favorable for these patients, we suggest that its complications, such as ocular toxicity, need to be carefully considered prior to treatment.

레이저 마이크로 접합 기술

김주한,이철구,Kim, Joo-Han,Lee, Chul-Ku 대한용접접합학회 2007 대한용접·접합학회지 Vol.25 No.2

생산 및 오염 통제를 위한 유인일치 (誘引一致) 조세 / 보조금제도에 대한 연구

김주한,김재철 ( Joo Han Kim,Jae Cheol Kim ) 한국경제학회 1996 經濟學硏究 Vol.44 No.2

초기상태가 불량한 자발성 뇌출혈 환자의 예후 - 70세 이상의 고령환자를 대상으로 -

김주한,이자규,임동준,권택현,박정율,정흥섭,이훈갑,서중근,Kim, Joo Han,Lee, Ja Kyu,Lim, Dong Jun,Kwon, Tack Hyun,Park, Jung Yul,Chung, Hung Seob,Lee, Hoon-Kap,Suh, Jung Keun 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.2

Objective : The prognosis of spontaneous intracerebral hemorrhage often depends on initial neurologic condition, size and location of hemorrhage and associated intraventricular hemorrhage. However, age of patient, coagulation state and other associated vascular diseases may also play a role when present. In recent years, the geriatric population has been increasing. The age distribution of the patients with intracerebral hemorrhages also has been increased, accordingly. However, such patients, especially when associated with poor initial conditions often tend to be managed rather conservatively. The authors analyzed retrospectively on forty-five patients with spontaneous intracerebral hemorrhage over the seventies with poor initial condition to find out whether there exists a difference of outcome between surgery and non-surgery group. Material and Method : A total of 45 patients over seventies with spontaneous intracerebral hemorrhage with Glasgow Coma Scale(GCS) 4-8 treated over last six years were included. The validity of surgical management for these patients as well as clinical variables which might have been operated on the outcome of these patients were evaluated. The Glasgow Outcome Scale(GOS) after three months was used for comparison of outcome. Results : In surgical group(19 cases), mean age was 74.5 years old, mean hematoma volume 67.2ml and mean GCS score 5.7 points. In nonsurgical group(26 cases), mean age was 79.3 years old, mean hematoma volume 32.1ml, and mean GCS score 6.8 points. Mortality rate in surgical group was 47.4%(9 patients), including 2 cases of post-operative rebleeding, while that in nonsurgical group was 46.2%. However, when patients with initial GCS 4-6 points and over 30ml in hematoma volume were regrouped, mortality rate in surgical group was 46.2%, whereas mortality rate in nonsurgical group was 66.7%. Conclusion : It is concluded that the mortality rate is much low in surgery group with initial GCS less than 6 points and hematoma volume over 30cc. There was no significant difference of outcome in patients with basal ganglia and thalamic hemorrhage. However, surgical treatment lowered the mortality and morbidity rate in patients with subcortical and cerebellar hemorrhage.

폴리머 마이크로 장치에 대한 레이저 투과 마이크로 접합

김주한,신기훈,Kim, Joo-Han,Shin, Ki-Hoon 대한용접접합학회 2005 대한용접·접합학회지 Vol.23 No.5

A precise bonding technique, transmission laser bonding using energy transfer, for polymer micro devices is presented. The irradiated IR laser beam passes through the transparent part and absorbed on the opaque part. The absorbed energy is converted into heat and bonding takes place. In order to optimize the bonding quality, the temperature profile on the interface must be obtained. Using optical measurements of the both plates, the absorbed energy can be calculated. At the wavelength of 1100nm $87.5\%$ of incident laser energy was used for bonding process from the calculation. A heat transfer model was applied for obtaining the transient temperature profile. It was found that with the power of 29.5 mW, the interface begins to melt and bond each other in 3 sec and it is in a good agreement with experiment results. The transmission IR laser bonding has a potential in the local precise bonding in MEMS or Lab-on-a-chip applications.

조선그리스도교련맹 『성경전서』(1990/2010) 신약성경에 대해 분석적 평가(V): 요한계시록

김주한 ( Joo Han Kim ) 한국대학선교학회 2021 대학과 선교 Vol.47 No.-

본 논문은 조선그리스도교련맹의 『성경전서』(1990/2010) 요한계시록을 분석적으로 평가한다. 『성경전서』는 대한민국의 『공동번역 성서』(1977)를 개정한 것으로 (민영진에 의해) “공동번역 성서의 평얀 교정본”이라고 명명되기도 했다. 그 이유는 『성경전서』의 편집자들이 고유명사의 변경, 맞춤법상의 교정, 어휘 변경, 대우법 조정, 문장 전체 교정, 편집상의 교정, 교정되지 못한 곳, 유사문미 탈락이라는 8개의 기준을 가지고 『공동번역 성서』를 개정했기 때문이다. 유사문미 탈락이라는 기준을 제외한 나머지 7개의 변화들이 『성경전서』의 요한계시록 본문에서 발견된다. 이 사실은 조선그리스도교련맹의 『성경전서』가 일단의 체계적이고 신중한 개정작업의 결과물임을 말해 준다. 그러나 본 논문은 새롭고 개정된 헬라어 비평본들의 출현(예. 많은 본문 변화를 담고 있는 NA28/UBS5와 2022년에 출판되기로 예고된 성경)과 남북한 통일 시대를 준비하기 위한 새로운 북한어 성경이 요구된다는 점을 지적한다. This article tries to analytically evaluate on the Revelation of John in the Bible(1990/2010) of the Korean Christian Federation. This Bible adapted The Common Translation(1977) of South Korea, and is commonly called the “Pyeongyang Recension of The Common Translation”(Dr. Young Jin Min’s word) because it was adapted in base of the following eight principles, i.e. changes of proper names, changes of orthography, changes in vocabularies, changes in honorific style, changes in editing marks, some non-corrected errors and homoioteleuta from The Common Translation. Except the homoioteleuta, the above-mentioned changes are found in the Revelation of John of the Bible of the Korean Christian Federation. This says that this Bible is a result of some systematic and considerable recension. However, this analysis is more contributive to indicate that a new translation in North Korean is requested because of both an advent of new and revised Greek New Testament editions( eg. NA²⁸/UBS⁵ and the next edition in 2022 containing many textual changes) and demands for a Bible to prepare the era of reunification of South and North Koreas.

H.264/AVC 병렬 MB Search를 이용한 3D Encoder

김주한(Joo-Han Kim),김진상(Jin-Sang Kim),조원경(Won-Kyung Cho) 한국정보기술학회 2011 Proceedings of KIIT Conference Vol.2011 No.5

3D 영상을 압축하기 위해서는 시간적 중복을 이용한 motion estimation(ME)과 인접 영상간의 중복을 이용한 disparity estimation(DE)방법이 필요하다. ME와 DE는 비교하는 대상이 다를 뿐 압축 알고리즘은 동일하다. 압축 알고리즘 중 H.264/MPEG-4 AVC의 variable block size ME(VBSME)는 이전의 fixed block size ME(FBSME)보다 더 높은 압축 효율을 보여주었다. 하지만 기존의 VBSME의 macro block(MB)선택 방법은 16by16 MB에서 4by4 MB로 선택하기 때문에 block size선택 속도가 느리다는 단점이 있다. 이 문제를 해결하기 위해서는 모든 경우의 MB size를 동시에 계산하여 선택해줘야 한다. 이 방법을 이용하여 VBSME와 VBSDE를 Pipelined Hardware Architecture기반의 SOC로 설계한다. We need to motion estimation(ME) and disparity estimation(DE) for 3D image compression.. ME and DE as a different compression algorithms to compare the target is the same. Of compression algorithms, H.264/MPEG-4 AVC of the variable block size ME (VBSME) of the previously fixed block size ME (FBSME) showed a higher compression efficiency. However, the existing VBSME macro block (MB) 16by16 MB How to choose a selection from the 4by4 MB block size selection is slow. To solve this problem, you calculate and choose the MB size at the same time. Using this method, a Pipelined Hardware Architecture VBSDE VBSME and as SOC-based designs.