간헐성 복통과 구토를 주소로 하는 중장염전이 동반된 장 이상회전 1례

김종식,정주영,박동철,김상우,김홍주,김영훈,Kim, Jong-Shik,Chung, Ju-Young,Park, Dong-Churl,Kim, Sang-Woo,Kim, Hong-Joo,Kim, Young-Hoon 대한소아소화기영양학회 2002 Pediatric gastroenterology, hepatology & nutrition Vol.5 No.1

저자들은 간헐적인 구토와 복통을 주소로 내원한 6세 된 남아에서 상부 위장관 조영술과 복부단층촬영으로 진단된 중장염전을 동반한 장 이상회전 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Intestinal malrotation is a developmental anomaly resulting from embryologic failure of fixation and rotation of the gut and predisposed to midgut volvulus and small bowel obstruction. Acute midgut volvulus is most often encountered in the newborn period. But older children and adults may have a history of intermittent episodes of partial volvulus presenting with recurrent colicky abdominal pain accompanying bilious vomiting. We experienced a case of intestinal malrotation complicated by midgut volvulus presented with recurrent vomiting and abdominal pain in a 6-year-old boy.

메타프로테오믹스의 미생물생태학적 응용

김종식,우정희,김준태,박년호,김충곤,Kim, Jong-Shik,Woo, Jung-Hee,Kim, Jun-Tae,Park, Nyun-Ho,Kim, Choong-Gon 한국미생물학회 2010 미생물학회지 Vol.46 No.1

미생물 군집과 기능연구를 위한 최근의 새로운 분석기술의 발전은 다양한 유전 관련 정보를 제공해왔다. mRNA를 포함하는 핵산을 기초로 한 연구를 뛰어넘어서 메타프로테오믹스는 미생물 군집의 유전형 및 표현형의 특징적인 정보를 보다 정교하게 제공할 수 있다. 이미 서로 다른 미생물 생태계인 해수, 인간의 배설물, 활성 슬러지, 산성 광산 폐수 생물막, 토양 등에 메타프로테오믹스 기술이 유용하게 사용되었다. 이들 연구는 여러 측면에서 상당히 다르지만 미생물 군집의 구조, 기능, 생리, 상호관계, 생태, 진화적 측면을 결정적으로 상호 연결한다는 것을 밝혀냈다. 본 총설은 메타프로테오믹스에 대한 현재까지의 가장 최신의 정보를 요약하여 제공함으로써 메타프로테오믹스에 대한 정확한 이해와 활용을 통해 다방면의 메타프로테오믹스가 가능하도록 하고자 하였다. New technologies are providing unprecedented knowledge into microbial community structure and functions. Even though nucleic acid based approaches provide a lot of information, metaproteomics could provide a high-resolution representation of genotypic and phenotypic traits of distinct microbial communities. Analyzing the metagenome from different microbial ecosystems, metaproteomics has been applied to seawater, human guts, activated sludge, acid mine drainage biofilm, and soil. Although these studies employed different approaches, they elucidated that metaproteomics could provide a link among microbial community structure, function, physiology, interaction, ecology, and evolution. These approaches are reviewed here to help gain insights into the function of microbial community in ecosystems.

Membrane Microarray를 이용한 Resveratrol에 의해 차별적으로 발현되는 유전자 군의 분석

김종식,장민정,김효은,김순영,김병오,손호용,Kim, Jong-Sik,Jang, Min-Jung,Kim, Hyo-Eun,Kim, Soon-Young,Kim, Byung-Oh,Sohn, Ho-Yong 한국생명과학회 2007 생명과학회지 Vol.17 No.8

본 연구에서는 다섯 종류의 phytochemical (resveratrol, genistein, epicatechin gallate, diaIly disulfide, caffeic acid phenetyl ester)과 sulindac sulfide가 암 억제 단백질 p53을 유도할 수 있는지에 대해 연구하였다. 처리한 모든 phytochemical에 의해 p53 단백질의 발현이 강하게 유도된 반면, sulindac sulfide에 의해서는 p53 단백질이 유도되지 않았다. 처리한 phytochemical 중 포도껍질이나 와인에 많이 들어있는 resveratrol에 의해 p53 단백질이 농도의존적 혹은 처리시간 의존적으로 증가 발현되는 것을 확인하였다. 암 억제 단백질인 p53 하위 단계의 유전자들만 집적되어 있는 membrane microarray를 이용하여 실험을 수행한 결과, 25개의 유전자가 up-regulation 된 반면, 2개의 유전자가 down-regulation 되는 것을 확인하였다. Up-regulation 되는 유전자중 4개를 선택하여, RT-PCR을 수행한 결과 모두 membrane microarray 실험의 결과와 일치하였다. 게다가 p53 null인 HCT116 세포주를 이용한 RT-PCR을 통하여 TSP-1 유전자의 발현은 p53 의존적이지 않은 반면, MASPIN 유전자는 p53 의존적임을 확인하였다. 이러한 연구 결과는 resveratrol에 의한 화학적 암 예방법의 분자생물학적 기전을 이해하는데 도움을 줄 것으로 기대된다. In the present study, we investigated whether several phytochemicals (resveratrol, genistein, epicatechin gallate, dially disulfide, caffeic acid phenetyl ester) and sulindac sulfide could induce expression of tumor suppressor p53 protein in human colorectal HCT116 cells. We found that p53 was dramatically induced by all phytochemical treatments except sulindac sulfide. Among treated phytochemicals, we selected resveratrol for further experiments because it is one of the highest p53 inducer. Using a Western blot analysis, we found that resveratrol induced p53 in a dose- and time-dependent manner. Additionally, using membrane-based microarray analysis, we found that twenty-five genes were up-regulated and two genes were down-regulated by resveratrol treatment. Among the up-regulated genes, we selected 4 genes and performed reverse-transcription-PCR to confirm microarray data. The results of RT-PCR were highly accorded with those of membrane microarray. In addition, we found that thrombospondin-1 (TSP-1) expression was not dependent on p53 presence, whereas mammary serine protease inhibitor (MASPIN) expression was dependent on p53 expressed by resveratrol treatment. The results of this study may help to promote our understandings of the molecular mechanisms of chemoprevention that are mediated by resveratrol in human colorectal cancer.

흰쥐에서 대사작용 억제에 의한 혈중 Mg²⁺ 조절

김종식,김상진,김진상,Kim, Jong-shick,Kim, Shang-jin,Kim, Jin-shang 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.1

Magnesium ($Mg^{2+}$) plays an important role in the regulation of a range of intracellular processes. Regulation of extracellular $Mg^{2+}$ contents was studied in the anesthetized Sprague-Dawley (SD) rats. Animals were injected intraperitoneally with sodium nitrite ($NaNO_2$), and circulating $Mg^{2+}$($[Mg^{2+}]c$) was measured after the injection and then 10 and 20 minutes later. A dose-dependent increase in $[Mg^{2+}]c$ was observed in animals injected with $NaNO_2$ at a dose of 10mg/kg or higher. Pretreatment with methylene blue prevented the $NaNO_2$-induced increase in $[Mg^{2+}]c$. $[Mg^{2+}]c$ displayed an inverse linear correlation with hemoglobin and exponential correlation during $NaNO_2$ injection. Injection of KCN or rotenone also induced an increase in $[Mg^{2+}]c$. An increase in $[Mg^{2+}]c$ was observed when respiration rate was reduced from 100/min (140ml/min) to 10/min (14ml/min) during 30 min. These results indicate that changes in $[Mg^{2+}]c$ inversely reflect alteration of ATP in a model of metabolic inhibition.

Correction of Dose Distribution at Total Body Irradiation using Compensator

김종식,조현상,김영곤,조정근,주상규,박영환,Kim Jong Sik,Cho Hyun Sang,Kim Young Kon,Cho Jung Keun,Ju Sang Kyu,Park Young Hwan 대한방사선치료학회 1997 大韓放射線治療技術學會誌 Vol.9 No.1

The using of compensator is required to adjust the irregular dose distribution due to irregular thickness of the body in Total Body Irradiation. Aluminuim, copper or lead is generally used as compensator. In our study, we would like to introduce a result of the attenuation and compensation effect of radiation use compensator made by duralumin and its clinical use. The thickness of compensator was calculated by the attenustion of radiation, which was measured by polystyrene phantom and ionization chamber(farmer). The compensation effect of radiation was measured by diode detector. All of conditions were set as in real treatment, and the distanc from source to detector was 446 cm. We also made fixation of device to easily attach the compensator to LINAC. Beam spoiler was menufactured and placed on the patient to irradiate sufficient dose to the skin. diode detector were placed on head, neck, chest, umbilicus. pelvis and knee with each their entranced exit points, and datas of dose distribution were evaluated and compared in each points for eleven patients(Feb. 96-Feb. 97). The attenuation rate of irradiation by duralumin compensator was measured as $1.4\%$ in 2mm thickness. The mean attenuation rate was $1.3\%$ per 2mm as increasing the thickness gradually to 50 mm. By using duralunim compensator, dose distribution in each points of body was measured with ${\pm}2.8\%$ by diode detectior. We could easily calculate the thickness of compensator by measuring the attenuation rate of radiation, remarkably reduce the irragularity of dose distribution duo to the thickness of body and magnify the effect of radiation therapy.

정상 치근과 치주질환에 이환된 치근면의 Electron Probe Microanalysis와 주사전자 현미경에 의한 연구

김종식,김종여,임성빈,정진형,Kim, Jong-Sig,Kim, Chong-Yeo,Lim, Sung-Bin,Chung, Chin-Hyung 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.2

Root surfaces affected by periodontal disease undergo various forms of changes. Cementum exposure from gingival recession may result in absorption of calcium, phosphorus, and fluoride and subsequent hypermineralization and increased radiodensity. Although some reports have suggested that inorganic content with root cementum might show various changes depending upon age or extent of periodontal disease, but no consensus can be reached regarding the the distribution of various elements. The present study examines the difference in mineral content between healthy and periodontal diseased roots by analyzing three areas per tooth along the root surface in cervico-apical direction using electron probe and scanning electron microscope. Healthy tooth that was extracted for orthodontic purpose was used as control. Experimental teeth include those with periodontal pocket depth exceeding 6mm and those with gingival recession and periodontal pocket depth of 2-4mm. Levels of Ca, P, Mg and Na were measured using wavelength dispersive x-ray spectrometer at three areas per tooth. The examined areas were located apical to cemento-enamel junction in control and periodontal ligament-depleted areas in experimental teeth. The corresponding areas were also examined with scanning electron microscope(x70) The results are as follows. 1. Minerals were detected in order of Ca, P, Mg and Na. In all root surfaces, levels of Ca and P were higher in dentin than in cementum. 2. Level of Mg was twice as high in dentin than in cementum. There was no significant difference in the level of Mg and Na between normal and periodontal diseased roots or between the various locations in the same root. 3. Level of Ca and P in the surface cementum showed no difference between normal and periodontal diseased root, although the areas in dentin with high level of either ion also showed high level of corresponding ion in cementum. 4. Difference in the Ca and P content between various locations within the same root was noted, although no coherent pattern existed. These results suggest that although the mineral content of the root cementum in periodontitis-affected tooth is affected by exogenous ions from saliva and food, but there was no difference in the mineral contents between normal and periodontally diseased root.

치이즈 숙성에 관한 연구 4 . 체다 치이즈 숙성중 질소화합물의 변화

김종식,장주익,김영교 ( J . S . Kim,J . I . Chang,Y . K . Kim ) 한국축산학회 1976 한국축산학회지 Vol.18 No.2

This experiment has been carried out td study the changes of caseins occurring in Cheddar cheese during ripening. The contents of total N, water soluble N, 12% TCA soluble N, and 5% NaCl soluble N were analyzed at intervals of 2 months and compared each other. Furthermore, total cheese casein, water soluble nitrogenous compounds, and 5% NaCl soluble nitrogenous compounds were fractionated by the use of DEAE-cellulose column. The results obtained were as follows: 1. The amounts of water soluble nitrogen in the cheese ripened for 2 and 4 months were 21.96% and 28.9% for total nitrogen in the cheese, respectively. On the other land, the amounts of 12% TCA soluble nitrogen in the cheese ripened for 2 and 4 months were 8.73% and 14.50% for total nitrogen, respectively. 2. The amounts, of 5%b NaCl soluble nitrogen in the cheese increased to 42.27% for 2 months ripening, but it decrease3 to 23.61% at 4th month. 3. Caseins of Cheddar cheese luring ripening were fractionated into 5 major and several minor peaks. Also, Soluble nitrogenous compounds in water and 5% NaCl were fractionated into 5 major and several minor ones, respectively. 4. At 4 months of ripening, total cheese casein and seluble nitrogenous compounds in 5% NaCl showed 10 and 4 bands on polyacrylamide gel electrophoresis, respectively.

시론-수학자가 이룬 노력의 산물 잊지말자

김종식,Kim, Jong-Sik 한국과학기술단체총연합회 1989 과학과 기술 Vol.22 No.7

생물학적 난분해성물질을 함유한 매립장 침출수의 응집 및 오존처리에 관한 연구

김종식,최준호 ( Jong Shik Kim,Joon Ho Choi ) 한국공업화학회 1998 공업화학 Vol.9 No.3

분절화된 인간 배아에서 세포자연사와 Fas, Fas-ligand, Bax, Bcl-2 발현에 관한 연구

김종식,김명신,양현원,류재혁,윤용달,배인하,정병준,송현진,Kim, Jong-Sik,Kim, Myoung-Shin,Yang, Hyun-Won,Yu, Chai-Hyeock,Yoon, Yong-Dal,Bae, In-Ha,Jung, Byeong-Jun,Song, Hyun-Jin 대한생식의학회 2002 Clinical and Experimental Reproductive Medicine Vol.29 No.3

Objective : The present study was performed to investigate whether apoptosis occur in human embryos by annexin staining and detect the expression of Fas, Fas-ligand (FasL), Bax, and Bcl-2 in human fragmented embryos derived from IVF-ET by immunofluorescence and Western blot analysis. Materials and Methods: Using annexin staining, immunofluorescence and Western blot analysis on normal and fragmented embryos, we were able to detect apoptotsis and apoptotic gene products in fragmented embryos. Result: Phosphatidylserine (PS) translocation, the marker for apoptosis, were detected frequently in fragmented embryos. Bcl-2 and Bax protein were detected in both fragmented and non-fragmented embryos. When fragmented embryos compared to normal embryos, immunofluorescent intensity of Bcl-2 tended to be lower in fragmented embryos. Bax gene expression increased in the fragmented embryos compared to the normal embryos. This result supports a model in which the molar ratio of Bcl-2 to Bax determines whether apoptosis induced or inhibited in human embryo. Fas was highly expressed in human preimplantation embryos but not FasL. It suggests that embryo may undergo apoptosis by binding with FasL produced by follicular or immune cells. Conclusion: The over expression of Bax and Fas will trigger apoptosis to lead embryo fragmentation and change embryo to be nonviable.