한국산 강도래목 곤충 2종 ( Oyamia nigribasis , Kamimuria coreana ) 의 생활사와 우화 양상에 관한 연구

김종선,나철호 ( Jong Sun Kim,Chul Ho Ra ) 한국하천호수학회 1996 생태와 환경 Vol.29 No.2

A study was conducted to gain information on the life cycles and emergence patterns of Oyamia nigribasis and Kamimuria coreana collected from Mt. Mudung and Byongpung in Korea. Oyamia nigribasis and Kamimuria coreana were semivoltine species which were taken two years to complete their life cycle, but some individuals hatched lately were lasted three years. The emergence patterns of both species were the extended type and the adults of O. nigribasis emerged between April to August and K. coreana emerged May to August. They emerged in the night and spent about 30∼40 minutes. O. nigribasis had some delayed hatching or diapause because their early instars were occurred between February and March, but this was not clear in K. coreana.

탐진강 수계의 수서곤충 군집에 관한 연구

김종선,함순아,나철호 ( Jong Sun Kim,Soon Ah Ham,Chul Ho Ra ) 한국하천호수학회 1996 생태와 환경 Vol.29 No.2

The present study was performed to analysis of aquatic insects community at Tamjin river system from September 1994 to January 1995. The total taxa of aquatic insects was identified 93 species 72 genera 37 families in 9 orders. Among them major groups were Ephemeroptera (22 species) and Coleoptera (20 species). The fauna was dominated by Ephemeroptera (52.8%), Diptera (14.3%), and Trichoptera (12.8%). The seasonal species number of collected specimens was 57 in summer, 54 in autumn and 50 in winter. Mostly dominant species were belonged to Ephemeroptera, above all Uracanthella rufa dominated and its indices ranged from 0.34∼0.99. Diversity indices were 0.13∼3.62. According to the saprobic system based on the diversity indices of aquatic insects, all the sites were determined as β-mesosaprobic or origosaprobic area.

Cloning of subtilisin Carlsberg gene from Bacillus licheniformis

김종선,이은경,양철학,Kim, Jong-Sun,Lee, Eun-Kyeong,Yang, Chul-Hak 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.2

Bacillus licheniformis로부터 썹틸리신 칼스버그 효소의 유전정보를 담고 있는 유전자를 15-mer의 합성된 올리고뉴클레오티드를 probe로 사용하여 "in-situ colony hybridization technique"으로 찾아내었다. 제한효소 Pst1으로 절단된 chromosomal DNA를 pBR322의 Pst1부위에 연결시킨 후 이것으로 대장균을 형질전환시켜 얻은 3,500여개의 콜로니를 in-situ colony hybridization technique를 써서 조사한 결과 probe에 hybridization되는 양성콜로니를 한개 얻었다. 여기에서 얻은 재조합유전자를 pS113이라 명명하였으며 제한효소로 이를 분석해 본 결과 4.6Kb 정도의 유전자가 삽입되었고 제한효소들의 절단부위는 이마 보고된 Jacobs의 결과와 대개 일치하였다. 위에서 얻은 유전자를 Bacillus에서 발현시키기 위해 제한효소 Pst1으로 절단된 pS113을 pMK4의 Pst1부위에, 연결시킨 후 이것으로 Bacillus를 형질 전환시켰다. 형질전환된 Bacillus를 카세인 플레이트에서 프로테아제의 활성을 조사해 본 결과 유난히 큰 활성을 보이는 것이 얻어졌다. The gene encoding subtilisin Carlsberg from Bacillus licheniformis has been isolated by the "in-situ colony hybridization technique" using synthetic oligonucleotide as a probe. Pst1 digested chromosomal DNA was ligated into the Pst1 site of pBR322. The ligation mixture was used to transform E. coli and the transformants were screened by the in-situ colony hybridization technique. Out of 3,500 colonies screened, one colony was hyridized to the probe. The recombinant DNA of this positive clone was named pS113. Digestion of pS113 by restriction endonucleases showed that the size of the inserted DNA was about 4.6 Kb and the restriction map was nearly like that of Jacobs'. To express the gene in Bacillus, Pst1 digested pS113 was ligated into the Pst1 site of pMK4. The ligation mixture was used to transform B. licheniformis and the transformants were assayed on casein plate for protease secretion. We obtained a clony having a distinctively large halo.

Bacillus Licheniformis 로 부터 썹틸리신 칼스버그 유전자의 분리

김종선,이은경,양철학 ( Jong Sun Kim,Eun Kyeong Lee,Chul Hak Yang ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.2

The gene encoding subtilisin Carlsberg from Bacillus licheniformis has been isolated by the $quot;in-situ colony hybridization technique$quot; using synthetic oligonucleotide as a probe. Pstl digested chromosomal DNA was ligated into the Pstl site of pBR322. The ligation mixture was used to transform E. coli and the transformants were screened by the in-situ colony hybridization technique. Out of 3,500 colonies screened, one colony was hyridized to the probe. The recombinant DNA of this positive clone was named pS113. Digestion of pS113 by restriction endonucleases showed that the size of the inserted DNA was about 4.6 Kb and the restriction map was nearly like that of Jacobs`. To express the gene in Bacillus, Pstl digested pS113 was ligated into the Pstl site of pMK4. The ligation mixture was used to transform B. licheniformis and the transformants were assayed on casein plate for protease secretion. We obtained a colony having a distinctively large halo.

수서곤충을 이용한 탐진강 수계의 수질평가

김종선,함순아,나철호 ( Jong Sun Kim,Soon Ah Ham,Chul Ho Ra ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.2

This study was conducted to evaluate the biological water quality by aquatic insects community analysis in Tamjin river system. The total taxa of aquatic insects was identified in all sites 93 species. 71 genera, 37 families in 9 orders: In summer, 57 species, 43 genera, 24 families, 9 orders, in autumn, 54 species, 41 genera, 25 families in 7 orders, and in winter, 50 species, 41 genera. 26 families in 7 orders were recorded. According to the analysis of biological indices of dominance index(DI), diversity index(H`), pollution index(PI) and biotic index(BI), Water quality of majority in suveyed sites was either oligosaprobic or β-mesosaprobic. It was β-mesosaprobic in all sites except for site 5 in summer. Whereas in autumn, site 5, 6, 7, 10 were oligosaprobic and the rest were β-mesosaprobic. In winter, site 2, 4, 5, 6 and 7 revealed as oligosaprobic, site 10, 11, 12 and 13 as β-mesosaprobic, and site 8 as polysaprobic condition.

갑상샘암 환자에서 수술 및 치료과정에서 유도된 갑상샘 호르몬 결핍이 정서적 스트레스에 미치는 영향

김종선,최원정,장항석,이용상,오영자,석정호,Kim, Jong Sun,Choi, Won-Jung,Chang, Hang-Seok,Lee, Yong Sang,Oh, Young-Ja,Seok, Jeong-Ho 한국정신신체의학회 2012 정신신체의학 Vol.20 No.2

연구목적 : 갑상샘암 환자는 수술 및 방사성 요오드치료를 포함한 치료과정 동안 우울, 불안을 포함한 정서적 디스트레스(distress)를 경험할 수 있다. 이번 전향적 연구는 분화갑상샘암 환자의 치료과정 중 수술 전, 수술 후, 일시적 갑상샘 기능저하 상태의 세 시점에서 우울과 불안 정도를 비교하기 위해 시행되었다. 방 법 : 병원우울불안척도와 디스트레스온도계를 사용하여 41명의 분화갑상샘암 환자의 수술 전, 수술 후, 일시적 갑상샘 기능저하 상태의 우울, 불안 정도를 순차적으로 측정하였다. 전체 환자군을 대상으로 치료과정에 따른 디스트레스의 변화를 비교하였고, 디스트레스온도계로 측정한 스트레스 점수 4점을 기준으로 스트레스가 높은 군과 낮은 군으로 나누어 디스트레스의 변화를 비교하였다. 모든 통계분석은 Window용 IBM SPSS (PASW) 18.0을 이용하였으며 디스트레스의 변화는 반복측정 분산분석으로 분석하였다. 결 과 : 병원불안우울척도의 불안점수는 수술 전($6.93{\pm}3.97$)이 수술 후($4.22{\pm}2.92$) 및 일시적 갑상샘 기능저하상태($4.93{\pm}3.64$)에서 보다 유의하게 높았다. 한편, 스트레스가 높은 군과 낮은 군에서 병원우울불안척도의 불안점수는 측정시기별로도 유의한 차이를 보였고 두 집단 사이에도 유의한 차이를 보였고 병원우울불안척도의 측정시기별 차이는 유의하지 않았고 두 집단간의 차이만 유의하였다. 결 론 : 분화갑상샘암 환자의 치료과정에서 유도되는 갑상샘 호르몬 결핍은 분화갑상샘암 환자의 정서적 디스트레스에는 유의한 영향을 미치지 않는다. 분화갑상샘암 환자들에서의 정서적 디스트레스는 수술 전에 경험하는 불안감 및 염려와의 연관성이 높아보이며, 수술 전 정서적 디스트레스에 대한 평가와 스트레스 감소를 위한 개입이 필요하다. Objectives : Thyroid cancer patients may experience emotional distress during cancer treatment including surgical operation and radioactive iodine treatment. The aims of this prospective study were to investigate changes of anxiety and depressive symptoms in patients with differentiated thyroid cancer(DTC) under preoperative, postoperative and short-term hypothyroidism state. Methods : Using the Hospital Anxiety and Depression scale(HAD) and the Distress Thermometer, we sequentially assessed the levels of anxiety, depression and distress in 41 DTC patients at 3 time points such as preoperative state, postoperative state and short-term hypothyroidism state. Results : The HAD-anxiety score was significantly higher in preoperative state($6.93{\pm}3.97$) than postoperative state($4.22{\pm}2.92$) and short-term hypothyroidism state($4.93{\pm}3.64$). Any other significant change in depression or distress thermometer score was not observed. Especially, difference of HADS score between the distress and none-distress groups was significant in preoperative state and post-operative state, but the difference become not significant in the short-term hypothyroidism state. Conclusions : Induced thyroid hormone deficiency during cancer treatment does not significantly affect emotional distress in patient with DTC. Anxiety and depression in these patients may be associated with distress of the patient before active cancer treatment.

일 지역 다문화 가족에 대한 인식도 조사 연구

김종선,박경순,김태용,Kim, Jong-Sun,Park, Kyung-Soon,Kim, Tae-Yong 한국가정간호학회 2009 가정간호학회지 Vol.16 No.1

Purpose & Method: Based on a large amount of research concerning the recognition of local residents in an era of an increasing number of multi-cultural families, this study aimed to provide fundamental data for program development and policies. As well, the data was stratified based on understanding of multi-cultural society, recognition of multi-cultural families, and social and economic adaptability of multi-cultural families. Result: The available literature supports the view that an understanding of a multi-cultural society and recognition of multi-cultural families encourages an emphasis on maintaining ethnicity and respect for diverse cultures. Parental approval for friendships with members of multi-cultural families tends to be encouraged, although there is still resistance to marital ties with such families. The literature indicates that immigrant women participate in economic activities of their adopted locale and adapt to the new societal structure. A positive correlation between recognition and interests in social and economic adaptiveness was apparent. Conclusion: Findings of the study suggest that complement, development, and application of realistic programs and institutional policies are required to understand multi-cultural families and establish a multi-cultural society. Ongoing research will be valuable in resolving outstanding questions and clarifying political alternatives.

Control of Flutter using ASTROS^* with Smart Structures

김종선,남창호,Kim, Jong-Sun,Nam, Changho 한국항행학회 2001 韓國航行學會論文誌 Vol.5 No.1

최근에 통합 설계 최적화 프로그램인 $ASTROS^*$와 Aeroservoelasticity(ASE) 모듈에 지능구조물의 해석 모듈을 통합하는 연구가 수행되었다. 통합된 소프트웨어를 이용해 플러터 억제 시스템을 설계하는 연구를 F-16모델을 이용해 수행하였으며 능동 제어 시스템을 위하여 신경망을 이용한 제어기가 설계되었다. 압전작동기에 의해 발생한 변형을 고려하기 위해 지능구조물 모듈은 $ASTROS^*$내의 열응력 해석 모듈을 개량하여 개발되었으며 ASE내에서 조종면을 이용한 입력과 압전작동기를 이용한 입력의 상호 호환성을 가능하게 하였다. 수치 예를 통해 개발된 제어시스템이 플러터속도를 증가시키는 데 효과적임을 보였다. Recent development of a smart structures module and its successful integration with a multidisciplinary design optimization software $ASTROS^*$ and an Aeroservoelasticity module is presented. A modeled F-16 wing using piezoelectric actuators is used as an example to demonstrate the integrated software capability to design a flutter suppression system. For an active control design, neural network based controller is used for this study. A smart structures module is developed by modifying the existing thermal loads module in $ASTROS^*$ in order to include the effects of the induced strain due to piezoelectric actuation. The control surface/piezoelectric equivalence model principle is developed, which ensures the interchangeability between the control surface force input and the piezoelectric force input to the Aeroservoelasticity modules in $ASTROS^*$. The results show that the developed controller can increase the flutter speed.

낸드 플래시 메모리를 위한 CLOCK 알고리즘 기반의 효율적인 버퍼 교체 전략

김종선,손진현,이동호,Kim, Jong-Sun,Son, Jin-Hyun,Lee, Dong-Ho 한국정보처리학회 2009 정보처리학회논문지D Vol.16 No.6

최근에 낸드 플래시 메모리는 빠른 접근속도, 저 전력 소모, 높은 내구성 등의 특성으로 인하여 차세대 대용량 저장 매체로 각광 받고 있다. 그러나 디스크 기반의 저장 장치와는 달리 비대칭적인 읽기, 쓰기, 소거 연산의 처리 속도를 가지고 있고 제자리 갱신이 불가능한 특성을 가지고 있다. 따라서 디스크 기반 시스템의 버퍼 교체 정책은 플래시 메모리 기반의 시스템에서 좋은 성능을 보이지 않을 수 있다. 이러한 문제를 해결하기 위해 플래시 메모리의 특성을 고려한 새로운 플래시 메모리 기반의 버퍼 교체 정책이 제안되어 왔다. 본 논문에서는 디스크 기반의 저장 장치에서 우수한 성능을 보인 CLOCK-Pro를 낸드 플래시 메모리의 특성을 고려하여 개선한 CLOCK-NAND를 제안한다. CLOCK-NAND는 CLOCK-Pro의 알고리즘에 기반하며, 추가적으로 페이지 접근 정보를 효율적으로 활용하기 위한 새로운 핫 페이지 변경을 한다. 또한, 더티인 핫 페이지에 대해 콜드 변경 지연 정책을 사용하여 쓰기 연산을 지연하며, 이러한 새로운 정책들로 인하여 낸드 플래시 메모리에서 쓰기 연산 횟수를 효율적으로 줄이는 우수한 성능을 보인다.

사출성형공정 모니터링용 엣지 디바이스 개발 및 평가

김종선,이준한,Kim, Jong-Sun,Lee, Jun-Han 한국금형공학회 2020 한국금형공학회지 Vol.14 No.4

In this study, an edge device that monitors the injection molding process by measuring the mold vibration(acceleration) signal and the mold surface temperature was developed and evaluated its performance. During injection molding, signals of the injection start, V/P switchover, and packing end sections were obtained through the measurement of the mold vibration and the injection time and packing time were calculated by using the difference between the times of the sections. Then, the mold closed and mold open signals were obtained using a magnetic hall sensor, and cycle time was calculated by using the time difference between the mold closed time each process. As a result of evaluating the performance by comparing the process data monitored by the edge device with the shot data recorded on the injection molding machine, the cycle time, injection time, and packing time showed very small error of 0.70±0.38%, 1.40±1.17%, and 0.69±0.82%, respectively, and the values close to the actual were monitored and the accuracy and reliability of the edge device were confirmed. In addition, it was confirmed that the mold surface temperature measured by the edge device was similar to the actual mold surface temperature.