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하지홍,정운복,이상로,탁연빈,김종봉 ( Ji Hong Ha,Woon Bok Chung,Sang Lo Lee,Ryun Bin Tak,Jong Bong Kim ) 한국유전학회 1991 Genes & Genomics Vol.13 No.4
The Sapsaree is a nearly extincted Korean native dog whose historic habitat has included much of the Southeastern Korea. The only existing population of Sapsaree consists of 50 dogs living in the small area of Kyung-San Goon in Kyung-Pook Province. Field observations of the population indicated the presence of two distinct morphological phenotypes that are divided into Blue- and Yellow-Sapsaree. A RFLP (Restriction Fragment Length Polymorphism) analysis using pV47-2 probe and Sapsaree DNA indicated the existance of two distinct genetic stocks concordant with the morphological phenotypes.


삽사리의 가계 분석에 사용할 수 있는 DNA probe 의 선정
이상로,이호섭,정운복,탁연빈,박희천,김종봉,하지홍 ( Sang Lo Lee,Ho Seob Lee,Woon Bok Chung,Ryun Bin Tak,Hee Cheon Park,Jong Bong Kim,Ji Hong Ha ) 한국유전학회 1991 Genes & Genomics Vol.13 No.4
In order to breed the Korean native dog, sapsaree, which exist as a single population in KyungPook province, we are going to use DNAfingerprinting as a tool for studying the pedigree. In this experiment, 4 kinds of DNA probes, Exon 8, Intron B, M13, and pV47-2 were analysed by means of conventional Southern hybridization with Sapsaree genomic DNAs digested with restriction enzymes for their usefulness as probe. On the basis of their autoradiographic band patterns and resolution, pV47-2 was determined to be the best probe for studing the pedigree of sapsaree.
김종봉,윤인숙,옥현숙,탁영빈,하지홍 대구효성가톨릭대학교 자연과학연구소 1992 基礎科學硏究論集 Vol.1992 No.1
진도개와 삽사리 혈청 lactate dehydrogenase와 혈청 alkaline phosphatase의 동위효소 및 효소활성도를 분석하였다. 전기영동결과 진도개와 삽사리의 혈청에서는 5가지 종류의 LDH의 동위효소가 모두 확인되었다. LDH의 활성도는 진돗개의 경우 522.53 ± 279.96(U/L)이었고 삽사리는 534.10 ± 280.35(U/L)이었다. 진도개와 삽사리의 혈청 alkaline phosphatase전기영동상에서는 한 종류의 동위효소만 관찰되었고 활성도는 진도개의 경우 7.61 ± 4.52(K-A unit)였고 삽사리는 10.46 ± 7.10(K-A unit)였다. 삽사리의 ALP 활성도는 연령에 따라 커다란 차이를 나타내었다. Isozymes and activities of serum lactate dehydrogenase and serum alkaline phosphatase were examined in Korean Jindo dog and Sapsaree. The electrophoretic pattern of serum lactate dehydrogenase showed five isozymes. The activities of serum lactate dehydrogenase were 522.53 ± 279.96 and 534.10 ± 280.35(U/L) in Jindo dog and Sapsaree. One kind of isozymes was observed in electrophoretic phenotypes of serum alkaline phosphatase. The activities of serum alkaline phosphatase were 7.61 ± 4.52 and 10.46 ± 7.10 (K-A unit) in Jindo dog and Sapsaree. A significant difference of alkaline phosphatase was observed according to the age in Sapsaree.
하지홍,정운복,이상로,탁연빈,김종봉 대구효성가톨릭대학교 자연과학연구소 1991 基礎科學硏究論集 Vol.1991 No.1
The Sapsaree is a nearly extincted Korean native dog whose historic habitat has included much of the Southeastern Korea. The only existing population of Sapsaree consists of 50 dogs living in the small area of Kyung-San Goon in Kyung-Pook Province. Field observations of the population indicated the presence of two distinct morphological phenotypes that are divided into Blue- and Yellow-Sapsaree. A RFLP (Restriction Fragment Length Polymorphism) analysis using pV 47-2 probe and Sapsaree DNA indicated the existence of two distint genetic stocks concordant with the morphological phenotypes.
삽사리의 가계 분석에 사용할수 있는 DNA probe의 선정
이상로,이호섭,정운복,탁연빈,박희천,김종봉,하지홍 대구효성가톨릭대학교 자연과학연구소 1991 基礎科學硏究論集 Vol.1991 No.1
DNA지문법은 범죄감식, 친자감별, 가계분석, twin zygosity의 결정, 집단 유전학, 행동 생태학 등의 분야(jeffreys et al., 1985:Gill et al., 1985, 1987 : Lander, 1989:Neufeld and Colman, 1990)에서 널리 활용될 뿐만 아니라 멸종위기에 처한 동식물의 육종과 보존을 위해서도 없어서는 안되는 중요한 연구방법이 되었다.(Georges et al., 1988:Burke et al., 1989). 최근 미국에서는 멸종위기에 처한 퓨마와 매의 보존을 위해 이들 동물들에 대한 RFLP연구를 행했는데 현존집단내 혈통관계에 대한 중요한 정보를 얻을 수 있었다