Endosulfan 이 흰쥐체내의 Cytochrome P - 450 효소계에 미치는 영향

김인선(In Seon Kim),이강봉(Kang Bong Lee),심재한(Jae Han Shim),서용택(Yong Tack Suh) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.2

To investigate the effects of endosulfan on cytochrome P-450 enzymes in mouse(Balb c.), endosulfan was given by an intraperitoneal dose of 7.5 ㎎/㎏. The treatment of endosulfan increased the cytochrome P-450 content by 3.3 to 42 fold, cytochrome b_5 content by 2.3 to 3.8 fold, NADPH cytochrome P-450 reductase activity by 5.3 to 6.4 fold and total haem content by 3.1 to 3.6 fold of mouse liver after 48 hrs of intraperitoneal injection. Endosulfancytochrome P-450 absorption spectrum exhibited miximum at 387㎚ and 389 ㎚ and broad near 407 ㎚ in the liver microsome. Reduced P-450-CO spectrum of the liver microsome exposed by the treatment of endosulfan showed maximum at 449 ㎚ and 450 ㎚ compared to that of the control having maximum at 451 ㎚, which indicated endosulfan induced cytochrome P-450 new isozymes. Aldrin epoxidase activities in the mouse liver and kidney were increased by 2.8 and 2.1 fold by the treatment of endosulfan. Also 7-ethoxyresorufin dealkylase activities in the mouse liver and kidney were elevated by 1.7 and 1.8 fold by treatment of endosulfan.

생쥐에서 Cytochrome P-450 효소계에 의한 ${\alpha}$-Endosulfan의 시험관내 대사시험

김인선,이강봉,심재한,서용택,Kim, In-Seon,Lee, Kang-Bong,Shim, Jae-Han,Suh, Yong-Tack 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.5

생쥐(Balb/C) 간과 신장의 microsomal cytochrome P-450 효소계에 의한 ${\alpha}$-endosulfan의 시험관내 대사시험을 수행하였다. ${\alpha}$-Endosulfan은 endosulfan lactone(EL), endosulfan hydroxyether(EHE), endosulfan alcohol(EA), endosulfan sulfate(ES), endosulfan ether(EE) 그리고 ${\beta}$-endosulfan(${\beta}$-E) 등으로 대사되었으며 주요 대사산물은 간에서 EL(13.2%) 및 EA(11.5%)이었으며 신장에서 EA(17.4%) 및 EHE(19.3%)이었다. Microsome 배양액중 유기용매 추출성 대사산물은 63.4%이었으며 수용성 대사산물은 37.1%이었다. 수용성 대사산물은 EA(83.9%), EHE(4.5%) 그리고 ES(2.3%)로서 주요 수용성 대사산물은 EA이었다. Piperonyl butoxide는 ${\alpha}$-endosulfan으로부터 EE의 생성을 86%, EA의 생성을 92% 그리고 EHE, EL 및 ES의 생성을 대부분 저해하였다. In vitro metabolism study of ${\alpha}$-endosulfan by liver and kidney microsomal cytochrome P-450 monooxygenase system of the mouse(Balb/C) was performed. ${\alpha}$-Endosulfan was metabolized to endosulfan lactone(EL), endosulfan hydroxyether(EHE), endosulfan alcohol(EA), endosulfan sulfate(ES), endosulfan ether(EE) and ${\beta}$-endosulfan(${\beta}$-E). The main metabolites of ${\alpha}$-endosulfan were EL(13.2%) and EA(11.5%) in liver microsome and EA(17.4%) md EHE(19.3%) in kidney microsome. The $^{14}C$-activity of organic extractable fraction and water soluble fraction were 63.4% and 31.7% in liver micosome incubates respectively. The water soluble metabolites were EA(83.9%), EHE(4.5%) and ES(2.3). Piperonyl butoxide treatment inhibited the formation of EE by 86%, EA by 92% and EHE, EL and ES were barely formed.

생쥐 체내에서 $^{14}C-{\alpha}-Endosulfan$의 대사

김인선,이강봉,심재한,서용택,Kim, In-Seon,Lee, Kang-Bong,Shim, Jae-Han,Suh, Yong-Tack 한국환경농학회 1996 한국환경농학회지 Vol.15 No.1

생쥐 체내에서 $^{14}C-{\alpha}-Endosulfan$의 흡수, 분포, 대사, 배설 등의 동태를 구명하기 위하여 약제를 7.5mg $kg^{-1}$ 수준으로 복강주사 처리하여 수행한 시험 결과를 요약하면 다음과 같다. 1. 약제처리 후 4 일 이내에 처리 방사능의 63.9%가 소변을 통해 배설되었고, 그 후부터는 배설율이 일정하였다. 2. 약제처리 후 각 조직별 방사능의 분포는 처리 30분 이내에는 심장에 가장 높게 분포하였으며, 2시간에는 간과 신장에서 그 분포율이 현저하게 증가하였으며, 시간이 경과함에 따라 그 분포율은 점점 감소하였다. 3. $^{14}C-{\alpha}-endosulfan$은 생쥐 체내에서 ${\beta}-endosulfan({\beta}-E)$, endosulfan ether(EE), endosulfan sulfate(ES), 그리고 endosulfan alcohol(EA)로 대사되었으며 주요 대사산물은 간에서 EA(13.25%) 그리고 신장에서는 EHE(19.37%)였다. 4. 소변중에 배설된 대사산물로는 EA(43.21%), ES(4.78%), ${\beta}-E$(7.21%), EE(3.72%), EHE(18.04%) 등이었다. Absorption, distribution, excretion and metabolism of $^{14}C-{\alpha}-Endosulfan$[1,4,5,6,7,7-hexachloro-8,9,10-=trinorborn-5-en 2,3-ylenebismethylene]sulfite) were studied in male mouse(Balb/c) after single intraperitoneal treatment as the dose level of 7.5 mg/kg body weights. After treatment of $^{14}C-{\alpha}-endosulfan$, the radioactivity was rapidly excreted into the urine(63.9 %) within 4 days, thereafter the excretion ratio was constant. Radioactivity levels in the tissues was reached maximum 0.5 hr in heart, 2 hrs in liver and kidney after the treatment, then decreased with time. Endosulfan was metabolized to ${\beta}-endosulfan({\beta}-E)$, endosulfan ether(EE), endosulfan sulfate(ES), and endosulfan alcohol(EA). The main metabolites were EA(13.25 %) in liver and endosulfan hydroxyether(EHE)(19.37 %) in kidney. The urinary metabolites were EA(43.21 %), ES(4.78 %), ${\beta}-E$(7.21 %), EE(3.72 %) and EHE(18.04 %).

생쥐에서 Cytochrome P - 450 효소계에 의한 α - Endosulfan 의 시험관내 대사시험

김인선(In Seon Kim),이강봉(Kang Bong Lee),심재한(Jae Han Shim),서용택(Yong Tack Suh) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.5

In vitro metabolism study of α-endosulfan by liver and kidney microsomal cytochrome P-450 monooxygenase system of the mouse(Balb/C) was performed. α-Endosulfan was metabolized to endosulfan lactone(EL), endosulfan hydroxyether(EHE), endosulfan alcohol(EA), endosulfan sulfate(ES), endosulfan ether(EE) and β-endosulfan(β-E). The main metabolites of α-endosulfan were EL(13.2%) and EA(11.5%) in liver microsome and EA(17.4%) and EHE(19.3%) in kidney microsome. The ^(14)C-activity of organic extractable fraction and water soluble fraction were 63.4% and 31.7% in liver micosome incubates respectively. The water soluble metabolites were EA(83.9%), EHE(4.5%) and ES(2.3%). Piperonyl butoxide treatment inhibited the formation of EE by 86%, EA by 92% and EHE, EL and ES were barely formed.

전자기력을 이용한 Al/Steel 판재의 스폿 용접의 미세조직 및 기계적 특성에 관한 연구

김인봉(In-Bong Kim),심지연(Ji-Yeon Shim),강봉용(Bong-Yong Kang),우기도(Kee-Do Woo) 대한용접·접합학회 2015 대한용접학회 특별강연 및 학술발표대회 개요집 Vol.2015 No.11

HPLC 형광분석법을 통한 벼에서 Brassinolide 의 검정

심재한(Jae Han Shim),김인선(In Seon Kim),이강봉(Kang Bong Lee),서용택(Yong Tack Suh),(E . D . Morgan) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.1

To determine brassinolide in rice(Oriza sutiva L.) using HPLC equipped with fluoresence detector, a highly sensitive fluorescence reagent. 1-cyanoisoindole-2-m-phenylboronic acid, was synthesized from the reaction of o-phthaldehyde, m-phenylboronic acid and KCN, then was reacted with brassinolide. The formation ratio of brassinolide boronate exhibited 90% up at the ratio of 20 : 1(㎍/㎍) of 1-cyanoisoindole-2-m-phenylboronic acid and brassinolide respectively. The detection limit of brassinolide boronate with fluoresence detector was 0.16 ng. Brassinolide was detected in heading stage(biomass : 10 g) and panicle formation stage(biomass : 100 g) of the rice(Oryza sativa L.) with quantity of 0.8 ㎍ and 0.2 ㎍ respectively. However, brassinolide was not detected in blooming and elongation stage.

방전시간이 전자기 펄스 접합부에 미치는 영향에 관한 연구

심지연(Ji-Yeon Shim),김인봉(In-Bong Kim),박동환(Dong-Hwan Park),강봉용(Bong-Yong Kang) 대한용접·접합학회 2015 대한용접학회 특별강연 및 학술발표대회 개요집 Vol.2015 No.11

회전형 저온 플라즈마 시스템을 이용한 분말식품의 균일한 살균 연구

김명찬,박덕모,한진수,우인봉,김동후,장성은,윤찬석,김인,Kim, Myung Chan,Park, Duck Mo,Han, Jin Soo,Woo, In Bong,Kim, Dong Hoo,Jang, Seong Eun,Yoon, Chan Suk,Kim, In 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.2

In this study, a relatively effective process is used to sterilize Escherichia coli on the surface of micro-sized calcium citrate powder using nitrogen and argon as process gases in a low-temperature vacuum plasma treatment. The purpose of this study is to confirm and to introduce the effectiveness of homogeneous surface treatment for the sterilization of fine inorganic powder by the rotatable low-temperature RF plasma system designed by ourselves. The results of the test using 3M petrifilm showed that there were no remarkable spots in the case of the surface of plasma treated powder, whereas the untreated powder showed many blue spots, which indicating that the E. coli was alive. After 5 days, in the same samples, the blue spots were seen to be larger and darker than before, while the plasma-treated powder showed no changes. The results from FE-SEM analysis showed that the E. coli was damaged and/or destroyed by reactive species generated in the plasma space, resulting in the E. coli being sterilized. Furthermore, the sterilization effects according to the selected parameters ($N_2$ and Ar; flow rate 30 and 50 sccm) adapted in this study were mutually similar, regardless of such different process parameters, and this indicates that homogeneous treatment of powder surfaces could be more effective than conventional methods. Therefore, the plasma apparatus used in this study may be a practical method to use in a powerful sterilization process in powder-type food.

카드뮴 정맥주사시 랏트의 치사율과 간, 신에서의 양-반응관계

김인봉,박정덕,홍연표,장임원 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.4

To investigate the lethality and dose-response relationship in liver and kidney of rats at 48 hours after cadmium exposure, single doses of cadmium chloride were given to Sprague-Dawley male rats by tail-intravenous injection with several groups of doses ranging from 2 to 8 mg CdCl_2/kg for lethality and from 0.2 to 3.2 mg CdCl_2/kg for dose-response relationship. In addition, control was treated with distilled water by same method. Hepatic and renal cadmium concentrations were analyzed with I.C.P.(Inductively Coupled Plasm) Emission Spectrometry (Jobin Yvon, Model JY 24, France). Dosages over 4 mg/kg were relevant to LD_100 and LD_50 was calculated as 3.3 mg/kg at 48 hours after cadmium chloride exposure in rat. The concentration in liver and kidney were increased according to given doses, and dose-response relationship was expressed as log Cd in liver= 0.8799+0.913×log Dose (F=1116.1, r=0.9877) and log Cd in kidney= 0.2542+0.0653×log Dose (F=884.8, r=0.9845), respectively. Also, the correlation between hepatic and renal cadmium concentration was highly significant(p<0.01), and dose-response relationship was expressed as long Cd in kidney = -0.6649+1.0259×log Cd in liver (F=411.1, r=0.9676). I consider that this data will be useful for the further studying of low-dose cadmium toxicity.

자궁경부, 자궁내막 및 난소병변에서 Nucleolar Organizer Regions(NORs)에 관한 연구

허준용,김인선,홍성봉 고려대학교 의과대학 1989 고려대 의대 잡지 Vol.26 No.1

Nucleolar organizer regions (NORs) is a DNA loop which has a ribosomal RNA gene, and it can be readily demonstrated by argyrophilia of NORs-assocated proteins (Ag NORs). The number of Ag-NORs reflects the cellular activities and proliferation rates, and it is known that it correlates well with the DNA flow cytometrlc data. Recently, the Ag-NORs has been actively studied on many organs under the assumption that Ag-NORs may be useful in determining the malignant potential of the lesions. A silver staining technique to identify Ag-NORs was applied to eighty-one paraffin sections from the normal, premalignant and malignant lesions of uterine cervix, endometrium and ovary. The numbers of Ag-NORs in the nuclei of 100 cells of each case were counted on x1000 magnification, and the mean number of Ag-NORs for every 100 cells was calculated. The results were summarized as follows: 1. The mean numbers of Ag-NORs per nucleus in squamous metaplasia, mild, moderate and severe dysplasia of cervix were 1.41±0.29 (mean±S.E), 1.19±0.39, 1.31±0.49 and 1.40±0.40 respectively. In constrast, the mean numbers of Ag-NORs per nucleus in CIS (3 cases) was 2.46±0.30. In cervical invasive squamous cell carcinoma, the mean numbers of Ag-NORs in stage Ⅰa (5 cases), stage Ⅰb (5 cases), stage Ⅱa (7 cases) and more than stage Ⅱb(6 cases) were 2.69±0.41, 2.54±0.36, 2.61±0.22 and 2.63±0.24 respectively. The differences between the numbers of Ag-NORs in nuclei of squamous metaplasia and cervical squamous cell carcinemas were statistically significant (P <0.01-0.001). 2. In endometrium, the mean numbers of Ag-NORs per nucleus in proliferative phase (3 cases), secretory phase (5 cases), endometrial hyperplasia (5 cases) and endometrial carcinoma (9 cases) were 1.73±0.04, 1.44±0.09, 1.60±0.15 and 2.59±0.35 respectively. The difference between the numbers of Ag-NORs in nuclei of endometrial hyperplasia and endontetrial carcinoma was statistically significant (P<0.001). 3. Among mucinous and serous tumors of the ovary, the mean numbers of Ag-NORs per nucleus in benign (9 cases), borderline (9 cases) and invasive tumors (9 cases) were 0.91±0.11, 1.71±0.16 and 2.39 ± 0.31 respectively. The differences between these groups were statistically significant (P<0.001). In conclusion, firstly, the numbers of Ag-NORs is useful in differentiating between premalignant and malignant lesions in uterine cervix. But it has no correlation with the clinical stages of cervical cancer Secondly, the number of Ag-NORs is also useful in differentiating between endometrial hyperplasia and endometrial carcinoma. Lastly, the Ag-NORs are useful in classification of the ovarian common epithelial tumors into benign, borderline and malignant tumors.