보라우무 메탄올추출물의 HT-29 대장암세포 증식 억제 효과

김은지,박소영,홍지은,신민정,임순성,신현경,윤정한,Kim, Eun-Ji,Park, So-Young,Hong, Ji-Eun,Shin, Min-Jeong,Lim, Soon-Sung,Shin, Hyun-Kyung,YoonPark, Jung-Han 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.4

해조류는 다양한 생리 활성을 나타내는 성분들을 함유하고 있어 항산화효과 등 다양한 생리활성 효과를 나타내는 것으로 보고되고 있다. 본 연구에서는 식용 가능한 28종의 해조류 메탄올추출물 중 보라우무 메탄올추출물이 인간의 대장에서 유래한 암세포인 HT-29세포의 세포증식을 억제함을 밝혔고, 가장 강한 HT-29 세포 증식 억제 효과를 나타낸 보라우무 메탄올추출물의 대장암세포 증식억제 기전을 밝히고자 하였다. 보라우무 메탄올추출물을 HT-29 세포 배양액에 여러 농도$(0{\sim}20{\mu}g/mL)$로 첨가하여 세포를 배양한 경우 보라우무 메탄올추출물 처리 농도가 증가할수록 세포증식이 현저히 감소하였고, apoptotic cell수는 현저히 증가하였다. Apoptosis의 주요한 조절인자인 Bcl-2 family 단백질 중 Bcl-2는 보라우무 메탄올추출물에 의해 변화가 없었고 Bax는 고농도에서만 소폭 감소하였다. 반면, t-Bid 단백질 수준은 보라우무 메탄올추출물의 처리에 의해 현저하게 증가하였다 Bcl-2 family Protein과 더불어 apoptosis의 조절에 주요한 역할을 하는 caspase 단백질의 경우 보라우무메탄올추출물 처리에 의해 cleaved caspase-8, -9, -7, -3 단백질 수준이 현저히 증가하였고, cleaved PARP 단백질수준도 현저히 증가하였다. 또한, caspase-8의 활성화를 유도하는 Fas 단백질 수준도 보라우무 메탄올추출물에 의해 현저하게 증가하였다. 이 결과로부터 보라우무 메탄올추출물은 caspase-8의 활성 증가에 의한 미토콘드리아 막 투과성 변화와 caspase-7/-3 활성의 증가를 통해 apoptosis를 유도함으로써 암세포의 증식을 억제한다는 결론을 내릴 수 있다. 본 연구는 보라우무를 항암효과를 지니는 기능성식품이나 항암제로 개발할 수 있는 가능성을 제시하고 있으며 향후 보라우무를 이용한 제품의 개발을 위해서는 유효성분의 동정 및 그 성분의 작용 기전에 대한 면밀한 추가 연구가 필요할 것으로 보인다. In the present study, twenty eight marine algae species were evaluated for their antiproliferative effect on HT-29 human colon cancer cells. Among these, the methanolic extract of Symphyocladia latiuscula (SL Ex) showed the highest inhibitory activity on HT-29 cell growth. In this study, we examined the mechanism by which SL Ex inhibited the HT-29 cell growth. Cells were cultured with various concentrations of $(0{\sim}20{\mu}g/mL)$ SL Ex. The SL Ex substantially decreased the viable cell numbers and induced apoptosis of HT-29 cells in a dose-dependent manner Western blot analyses of total cell lysates revealed that SL Ex increased the levels of cleaved caspase-8, -9, -7, and -3, and poly (ADP-ribose) polymerase in HT-29 cells. In addition, SL Ex increased truncated Bid levels but moderately decreased Bax levels at only $20{\mu}g/mL$. Furthermore, SL Ex did not affect Bcl-2 protein levels but increased the levels of Fas in HT-29 cells. The present results indicate that SL Ex inhibits cell growth via inducing apoptosis in human colon cancer cells. The mechanism of apoptosis induction by SL Ex involves caspase-8 activation leading to changes in mitochondrial events and subsequent activation of the caspase-7/caspase-3 cascade. Our finding may lead to the development of new therapeutic strategies for the treatment of colon cancer.

식물공장에서 재배한 브로콜리 새싹 용매 분획물의 항산화 활성

김은지,김미혜,Kim, Eun-Ji,Kim, Mi-Hye 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.1

This study was designed to determine the antioxidant activity of solvent fractions of broccoli sprouts grown by controlling the growing environment at the plant factory system. Fractionation was achieved with chloroform, n-hexane, ethyl acetate, butanol, water by 70% EtOH extract of the broccoli sprouts. Each solvent fraction was put through TLC and HPLC to separate active components. Higher antioxidant activities were observed for the butanol and ethyl acetate layers. Further evaluation of each of the 5 layers (LH1 to LH5) of the butanol fraction showed that the refined LH3 extract had a high antioxidant effect. Components with similar Rf values from TLC had the same retention times and peaks in the HPLC analysis. It was also determined that the sulforaphane content was high at the chloroform and butanol layers and the sulforaphane was responsible for, the high antioxidant activity. Thus, to use for functional materials, the butanol extract/layer of broccoli sprouts is recommended as the most effective.

개 정액의 냉동보존 시 κ-Carrageenan이 정자 성상에 미치는 영향

김은지,전유별,유일정,Kim, Eun-Ji,Talha, Nabeel A.H.,Jeon, Yu-Byeol,Yu, Il-Jeoung 한국동물생명공학회 2019 한국동물생명공학회지 Vol.34 No.1

This study was conducted to find out the effect that ${\kappa}-Carrageenan$ has on the properties of dog sperm when it was added to the cryoprotectant. Extender basically was contained 1.21 g Trizma base, 0.67 g citric acid, 0.4 g glucose, 0.03 g penicillin G, 0.05 g streptomycin sulfate. Extender1 was added with 0.1%, 0.2%, 0.3%, and 0.5% carrageenan, while extender2 was supplemented with glycerol. After freezing-thawing, the motility, viability, acrosome integrity, apoptosis, and ROS (reactive oxygen specifications) of sperm were measured to analyze the effects of the supplementation of carrageenan. Total Motile (TM), Rapid Progressive Motile (RPM), Medium Progressive Motile (MPM), and Immotile were measured through the CASA system after thawing in 37 degree water. Extender with 0.2% ${\kappa}-carrageenan$ ($64.26{\pm}0.49$) was significantly higher than control ($40.24{\pm}8.27$) (p < 0.05). RPMs of extender with 0.1%, 0.2% ${\kappa}-carrageenan$ ($57.64{\pm}6.34$, $56.47{\pm}1.35$) were significantly higher than the other groups (p < 0.05). Acrosome integrity was measured by dyeing to PSA-FITC with an epifluorescence microscope. Normal acrosome ratio of extender with 0.5% ${\kappa}-carrageenan$ ($61{\pm}8.03$) was higher than the other groups (p < 0.05). Apoptosis was measured with a FACSCalibur flow cytometer using FITC (FITC Annexin V Apoptosis Detection Kit). Treated groups of ${\kappa}-carrageenan$ of 0.1% ($0.81{\pm}0.05$), 0.2% ($0.85{\pm}0.05$) were significantly higer (p < 0.05) than control. Modified SYBR/PI staining was used for determination of viability and DCF staining was used for evaluation of ROS. Viability and ROS were not significantly different from other groups. In conclusion, adding a certain concentration of carrageenan to the extender of cryopreservation, carrageenan contributes to the improvement of the sperm motility, acrosome integrity and prevention of apoptosis.

항암 치료 후 발생한 수족증후군에 대한 한의학적 치료의 효과에 대한 고찰

김은지,박찬란,손창규,조정효,이남헌,Kim, Eun-ji,Park, Chan-ran,Son, Chang-gue,Cho, Jung-hyo,Lee, Nam-hun 대한한방내과학회 2021 大韓韓方內科學會誌 Vol.42 No.3

Objectives: Hand-foot syndrome (HFS) is a common adverse effect of chemotherapy in cancer patients. This review synthesizes research results to assess the effect of traditional Korean medicine (TKM) on HFS in cancer patients. Methods: Four databases (PubMed, The Cochrane Central Register of Controlled Trials, Research Information Sharing Service, China National Knowledge Infrastructure) were searched for randomized controlled trials (RCTs) to assess the effects of TKM on HFS. The effects and quality of RCTs were assessed with the Cochrane risk of bias (ROB) tool. Results: Six RCTs met our study criteria. In all six, TKM showed improvement in HFS symptoms as well as quality of life scores when compared to the control group. However, the methodological quality of RCTs was relatively low due to the unclear or high ROB. Conclusions: TKM would be helpful to patients with HFS after chemotherapy. To clarify the clinical efficacy of TKM, well-designed studies are required in the future.

유해인자별 노출실태 및 분석방법 26 - 실내공기 중 포름알데히드(HCHO) 분석의 정확도 및 재현성 평가

김은지,Kim, Eun-Ji 대한산업보건협회 2013 산업보건 Vol.306 No.-

고온기 반밀폐형온실 냉방이 파프리카 생육과 광합성 특성에 미치는 영향

김은지,박경섭,구희웅,박가은,명동주,전용환,나해영,Kim, Eun Ji,Park, Kyoung Sub,Goo, Hei Woong,Park, Ga Eun,Myung, Dong Ju,Jeon, Yong Hwan,Na, Haeyeong 한국생물환경조절학회 2021 생물환경조절학회지 Vol.30 No.4

본 연구는 냉방이 가능한 반밀폐형온실과 일반 플라스틱온실에서의 정식 후 고온 스트레스가 파프리카에 미치는 영향 구명을 위해 수행하였다. 지열과 팬앤패드를 활용하여 냉방이 가능한 반밀폐형온실의 파프리카는 냉방이 되지 않는 3중 플라스틱 하우스의 파프리카보다 유의적으로 높은 광합성 속도를 보여 주었다. 플라스틱 하우스의 파프리카가 고온 스트레스에 의해 광합성 속도가 느려지는 것을 제시하고 있다. 초장은 반밀폐형온실이 13cm 더 높게 증가하였으며, 엽면적은 이식 후 2주차까지 생장 속도가 비슷하였으나 3주차 경과 시 반밀폐형온실이 플라스틱온실보다 47% 높은 차이를 보였다. 착과 수는 반밀폐형온실 10.6개/주, 플라스틱온실 4.6개/주가 착과하여 플라스틱온실 대비 반밀폐형온실이 130% 높게착과하였다. 과중 또한 반밀폐형온실과 플라스틱온실이 각각 566.7g/plant와 387g/plant으로 46% 차이를 나타냈다. 이상의 결과로 냉방이 가능한 반밀폐형온실에서 파프리카를 재배할 경우 일반 플라스틱온실보다 광합성과 생육이 양호하였음을 확인할 수 있었다. 따라서, 반밀폐형온실의 냉방 효율을 위한 요소기술을 일반 플리스틱온실에 적용하여 여름철 고온기를 극복한다면 수확량 및 품질 향상을 통한 농가소득 증대가 가능해질 것으로 기대된다. In this study, experiments were conducted to investigate the effects of high- temperature stress on paprika in a semi-closed greenhouse where cooling is available and a normal plastic greenhouse. Paprika grown in a semi-closed greenhouse in which geothermal cooling is provided showed a significantly higher speed of photosynthesis than paprika grown in a 3-layer plastic greenhouse in which there is no cooling system. It suggests that the photosynthesis speed of paprika in a plastic house decreases owing to high temperature stress. Plant height increased by 13cm more in the semi-closed greenhouse, and the size of leaf showed similar growth speed until the 2nd week after transplanting, however, after 3 weeks, the semi-closed greenhouse showed a big difference by 47% compared with the plastic greenhouse. In terms of the fruit count, the semi-closed greenhouse had 10.6 fruits/plant and the plastic greenhouse had 4.6 fruits/plant, indicating that the semi-closed greenhouse had a higher number of fruits by 130% than the plastic greenhouse. The fruit weight also presented a difference between the semi-closed greenhouse and the plastic greenhouse by 46%, which is 566.7g/plant and 387g/plant, respectively. According to the above mentioned results, it was validated that when paprika is cultivated in a semi-closed greenhouse where a cooling system is applied, photosynthesis and growth were better than in the normal plastic greenhouse. Thus, if the hot summer season is overcome by applying the elemental technologies for the cooling system to the normal plastic greenhouse, farm income may increase through improvement in the yield and quality.

황백(黃柏)이 토끼의 전립선요도압 및 혈압에 미치는 영향

김은지,한양희,Kim, Eun-ji,Han, Yang-hee 대한한방내과학회 2017 大韓韓方內科學會誌 Vol.38 No.2

Objective: This study was undertaken to examine the effect of Cortex Phellodendri on prostatic urethral pressure and mean arterial blood pressure of rabbits. Methods: To measure prostatic urethral pressure and mean arterial blood pressure, a Mikro-Tip catheter transducer was inserted and positioned in the prostatic urethra and left carotid artery. After a stabilizing period, phenylephrine ($1{\mu}/kg$) was intravenously administered two or three times to increase the urethral pressure and mean arterial blood pressure. Cortex Phellodendri (2.5 mg/kg and 5 mg/kg doses of Cortex Phellodendri extracted from 80% Ethanol) was administered intravenously, followed by phenylephrine, with no time interval between the doses. The urethral pressure and mean arterial blood pressure were then measured to determine whether they had stabilized. Results and Conclusion: Cortex Phellodendri appeared to inhibit phenylephrine-induced increases in prostatic urethral pressure and mean arterial blood pressure.

조등산(Diaoteng-san, Choto-san, 釣藤散)과 GB34(陽陵泉) 전침치료를 활용한 약인성파킨슨증후군 환자의 증례 1례

김은지,송호준,김현호,한양희,임정태,Kim, Eun-ji,Song, Ho-joon,Kim, Hyun-ho,Han, Yang-hee,Leem, Jung-tae 대한한방내과학회 2017 大韓韓方內科學會誌 Vol.38 No.5

Drug-induced Parkinsonism is the most frequently observed type among the cases of secondary Parkinsonism. Besides typical parkinsonian symptoms, such as tremor, rigidity and bradykinesia, drug-induced Parkinsonism manifests with additional simultaneous symptoms like orobuccolingual dyskinesia, mixed type of tremor (resting, action), and symmetry of expressions. We present a case of drug-induced Parkinsonism, affected by taking the antiulcer drug cimetidine. Jodeung-san extract (Choto-san, Tsumura Co. 10) was administered for 7 days and acupuncture (electronic, auricular, pharmacopuncture) was conducted 3 times. The clinical outcomes were then evaluated through the patient's global impression of change, visual analogue scale, and Hoehn and Yahr stage. After the treatment, the clinical features, such as tremor and orobuccolingual dyskinesia, disappeared. The combination of Jodeung-san and electro-acupuncture at GB34 could therefore be a remedy for the patients with drug-induced Parkinsonism.

해외 서점 탐방-일반 유통을 거치지 않은 모든 책이 '모사쿠샤' 서점

김은지,Kim, Eun-Ji 대한출판문화협회 1998 출판저널 Vol.236 No.-

유통기구를 통하지 않는 모든 책들을 갖춘 '모사쿠샤' 서점은 출판사와 단체, 독자 모두에게 환영받고 있다. 10평 남짓한 공간에 반핵.노동.장애인, 한국.북한관련서, 대학신문과 팜플렛 등이 주제별로 빼곡하게 들어차 있다.

간다거리의 스포츠 전문서점 '쇼센 북마트'

김은지,Kim, Eun-Ji 대한출판문화협회 1998 출판저널 Vol.238 No.-

올해로 개점 31년째인 '쇼센북마트'는 지하 1층 지상 4층에 스포츠 관련서와 비디오테이프.CD를 갖추고 있다. 축구.야구 등 인기종목뿐만 아니라 합기도.유도.태극권 등 격투기 같은 희귀종목 관련서는 타의 추종을 불허한다. 또 모든 스포츠 잡지의 과월호를 모두 갖추고 있어 스포츠광들의 발길이 끊이지 않는다.