진공 포장에 의한 식품의 저장 효과

김유라 ( You Ra Kim ),배동훈 ( Dong Hoon Bai ) 단국대학교 신소재기술연구소 2008 신소재 연구논집 Vol.16 No.-

초등 교과교육 : 물발자국 개념에 기반을 둔 초등학교 고학년용물 환경교육 프로그램 개발 및 효과분석

김유라 ( You Ra Kim ),이지은 ( Ji Eun Lee ),이상원 ( Sang Won Lee ) 서울敎育大學校 初等敎育硏究所 2014 한국초등교육 Vol.25 No.1

본 연구는 인간이 사용하는 물의 양의 나타내는 지표인 물발자국 개념에 기반을 둔 초등학교고학년용 물 환경교육 프로그램을 개발하여 초등학교에서 물의 소중함, 물 오염과 부족, 물의 보존과 같은 물 환경교육을 효과적으로 실시하는데 목표가 있다. 이를 위하여 초등학생의 학습자 발달단계뿐만 아니라 복잡한 시스템이나 인과관계에 대한 이해보다는 일상생활과 연계된 내용으로 프로그램을 개발하였다. 프로그램 개발은 물 환경교육과 물발자국에 대한 이론적 고찰, 물 환경교육 모형 설정, 교육프로그램 목표 및 주제 선정, 교육프로그램 교수·학습 과정안 개발 및 전문가 검토, 수정·보완 절차에 따라 수행하였다. 개발한 프로그램의 효과를 검증하기 위하여 초등학교 5학년 한 개 학급(30명)을 대상으로 창의적 재량활동시간에 적용하고, 물 환경에 대한 인식과 태도의 사전·사후 검사를 실시하였다. 본 연구의 결론은 다음과 같다. 첫째, 초등학교 고학년 물 환경교육에 활용할 8개 주제 16차시 프로그램을 개발하였다. 개발된 프로그램은 교수·학습 과정안, 활동지, 읽기 자료, 평가 자료로 구성된 교사용 교수자료와 활동이 포함된 활동지, 읽기 자료, 학습자용 자료로 구성하여 실제 수업에 사용될 수 있도록 하였다. 둘째, 개발된 환경교육 프로그램은 초등학교 고학년의 물 환경에 대한 인식과 태도를 향상시키는 교육적 효과가 있었다. 결론적으로 본 연구에서 개발된 교육프로그램은 초등학교 현장에서 최근 국제적으로 중요한 문제로 대두되고 있는 물 오염과 부족, 물의 보존 등과 관련한 물 환경교육을 수행할 수 있는 하나의 사례로서, 우리 사회가 지속가능한 사회로 나아갈 수 있도록 현장 교사들이 물 환경교육을 효율적으로 학생들에게 적용할 수 있는 자료를 제공하는데 그 의미가 있다. The purpose of this study was to develop a water footprint concept-based water environmental education program for the upper grades of elementary school, and to investigate the influence on the water environmental awareness and attitude of 5th graders in an elementary school in Gyeonggi-Do. To achieve the effectiveness of water environmental education containing precious water, pollution and shortages of water, the program developed and implemented the following 4 stages of preparation, development, verification, field test and evaluation. The experiment made progress as pretest-posttest control group design, and the program was applied to a class in creative discretionary activity hour for 8 weeks. A questionnaire was used to survey the effects of student`s awareness and attitude on water environment. The i-STATistics program was used to analyze the results. The major results were as follows. First, the developed program follows the three regions which are contents process of water conservation, practical part on water conservation based on water footprint concept and expanding environments principle in accordance with students` cognitive development stages. Second, the developed program is made up of eight parts: our life and precious water, water pollution and shortages, understanding of water footprints, closing & textiles life to reduce the water footprints, dietary life to reduce the water footprints, housing life to reduce the water footprints, reducing water footprints of school and local community life, and reducing water footprints of national and international society life. Third, the developed program had a positive effect on elementary school student`s awareness and attitude on water environment through quantitative and qualitative analysis. In conclusion, the developed program was an applicable example on water-related environmental education in the elementary school. The study provide to teaching material to proceed with effective class on water environmental education by field teacher toward sustainable society. Therefore, more intensive research needs to be conducted more intensively and systematically on the teaching and learning methods related to a water environmental education in elementary school.

후천 코눈물관 막힘에서 두 가닥 실리콘관 삽입술의 장기효과

김유라,안민,You Ra Kim,MD,Min Ahn,MD 대한안과학회 2012 대한안과학회지 Vol.53 No.11

Purpose: To evaluate the long term effectiveness of double silicone tube intubation in the treatment of acquired nasolacrimal duct (NLD) obstruction. Methods: The authors treated 68 eyes of 40 patients with acquired NLD obstruction. The resolution of preoperative symptoms and signs of NLD obstruction was evaluated 18 months after the operation. Results: The mean age of patients was 64.7 years and the mean follow-up period was 24.91 months. The symptoms and signs of NLD obstruction were improved in 85.29% of the patients. The partially obstructed group showed a higher success rate (89.29%) than the completely obstructed group (50.0%). A prolapse of silicone tubes to the ocular side was observed in 2 eyes, and 2 eyes had inflammatory signs after the procedure. Conclusions: Double silicone tube intubation is an effective primary therapy with NLD obstruction, especially for patiensts with simple partially obstructed NLD instead of dacryocystorhinostomy.

아크로모박테르 자일로속시단스에 의한 각막궤양 1예

김유라,유인천,이혜수,You Ra Kim,In Cheon You,Hye Soo Lee 대한안과학회 2011 대한안과학회지 Vol.52 No.11

Purpose: To report a case of corneal ulcer caused by Achromobacter xylosoxidans in a farmer. Case summary: A previously healthy 68-year-old man presented with unilateral redness and irritation after his eye was grazed by a cow’s tail. The patient had previously been treated in a local clinic for four days without improvement. Bacterial staining, culture, and an antibiotic sensitivity test were performed from a corneal scrape. The cultures revealed growth of A. xylosoxidans. The patient was treated with moxifloxacin and ceftazidime eyedrops. After three months of treatment, the infection was resolved with mild scarring. Conclusions: Although it is a rare pathogen, A. xylosoxidans should be considered as a potential pathogen in patients presenting with corneal ulceration due to trauma from an object contaminated by soil or animal feces and having a slowly progressive disease and localized infiltrate but showing Gram-negative bacilli on smear examination. J Korean Ophthalmol Soc 2011;52(11):1362-1365

생쥐를 이용한 스테로이드 유발 고안압 모델

김유라,강완석,권의용,조남천,이동욱,You Ra Kim,Wan Seok Kang,Eui Yong Kweon,Nam Chun Cho,Dong Wook Lee 대한안과학회 2014 대한안과학회지 Vol.55 No.8

Purpose: To determine whether rat eyes develop increases in intraocular pressure (IOP) in response to a topically applied corticosteroid and to investigate the relationship between ocular hypertension and apoptosis of retinal ganglion cells. Methods: IOP was monitored by rebound tonometry in a group of 10 rats that received topically administered dexamethasone in both eyes (experimental) and in another group of 5 rats that received artificial tears (control) three times daily for 4 weeks after the establishment of baseline IOP values. Only eyes that increased by more than 50% compared with the basal IOP were administered once per day for 5 weeks. After 8 weeks, selective immunofluorescence stain for retinal ganglion cells and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stain were conducted. Results: Among 20 experimental eyes, 11 eyes (55%) showed a greater than 50% increase in IOP compared with basal IOP. After 8 weeks, the mean IOPs for the experimental and control groups were 11.8 ± 1.4 mm Hg and 18.5 ± 1.0 mm Hg, respectively (<em>p</em> < 0.01). The counts of central retinal ganglion cells (RGCs) were 2718 ± 240 and 2612 ± 443, respectively (<em>p</em> = 0.294). The results of the TUNEL stain also showed no differences. Conclusions: Rat eyes exhibit a steroid-induced ocular hypertensive response with no local complications. However, maintaining ocular hypertension increased by 50% for two months was not enough to detect changes in RGCs. J Korean Ophthalmol Soc 2014;55(8):1202-1207

효모에서 Hrq1과 Rad14의 상호작용에 대한 연구

민문희,김민지,최유진,유민주,김유라,안효빈,김채현,권채연,배성호,Min, Moon-Hee,Kim, Min-Ji,Choi, You-Jin,You, Min-Ju,Kim, Uy-Ra,An, Hyo-Bin,Kim, Chae-Hyun,Kwon, Chae-Yeon,Bae, Sung-Ho 한국미생물학회 2014 미생물학회지 Vol.50 No.2

Hrq1은 곰팡이 유전체에서 생물정보분석에 의해 발견된 새로운 RecQ helicase이다. 이 단백질은 인간의 RECQL4와 가장 상동성이 높으며 최근의 유전학적 생화학적 연구를 통해서 유전체 안정성을 유지하는데 어떤 역할을 할 것으로 예상되었다. 본 연구에서는 RECQL4와 상호작용하는 것으로 알려진 인간 유전자들과 상동성이 있는 효모 유전자들이 Hrq1과 상호작용하는지를 yeast two-hybrid assay를 이용하여 조사하였다. 총 11개의 유전자를 조사한 결과, nucleotide excision repair (NER) 인자 중의 하나인 Rad14이 Hrq1과 상호작용하는 것을 발견하였다. 또한 정제한 단백질을 이용한 pull-down assay로 Hrq1과 Rad14 사이의 직접적인 상호작용을 확인하였다. Hrq1과 Rad14 사이의 yeast two-hybrid 상호작용은 4-nitroquinoline-1-oxide에 의한 DNA 손상으로 더욱 증가하였으며, 이러한 상호작용의 증가는 또 다른 NER 인자인 Rad4에 의존적이었다. 이러한 결과들은 Hrq1이 Rad14과의 상호작용을 통하여 NER 과정에 어떤 역할을 할 가능성을 제시하고 있다. Hrq1 is a novel member of RecQ helicase family, found in fungal genomes by bioinformatics analyses. It is most homologous to human RECQL4 and recent genetic and biochemical studies suggested that it may play roles in the maintenance of genome stability. In this study, we investigated yeast two-hybrid interactions between Hrq1 and the yeast genes homologous to the human genes that are known to interact with RECQL4. Among the 11 genes tested, Rad14, a nucleotide excision repair (NER) factor, was found to interact with Hrq1. In addition, pull-down assay with the purified proteins revealed direct protein-protein interaction between Hrq1 and Rad14. The yeast two-hybrid interaction was enhanced by the DNA damage induced by 4-nitroquinoline-1-oxide, which was dependent on the presence of Rad4, a key NER factor. These results suggest that Hrq1 may function in NER through interaction with Rad14.

급성폐쇄각녹내장 환자의 초기치료에서 수정체유화술과 레이저홍채절개술 비교

이창훈(Chang Hoon Lee),유인천(In Cheon You),김유라(You Ra Kim) 대한안과학회 2016 대한안과학회지 Vol.57 No.2

목적: 급성폐쇄각녹내장 환자들을 대상으로 수정체유화술과 레이저홍채절개술의 효과를 비교하고자 한다. 대상과 방법: 급성폐쇄각녹내장으로 진단 받은 환자 61명을 대상으로 하여 연구를 진행하였다. 레이저홍채절개술 혹은 수정체유화술을 실시하였으며, 연령, 성별, 치료 시행 전후 안압, 시력, 안압하강제의 사용 개수 및 치료 시행 후 합병증에 대해 술 후 하루, 한달, 6개월, 12개월째 후향적으로 조사하였다. 경과관찰 기간 내 레이저홍채절개술 및 수정체 유화술이 병합된 경우 및 섬유주절제술을 받은 군은 제외하였으며, 발병 후 3일 이내 시술을 시행한 경우만 포함하였다. 결과: 총 61명의 환자 중 45명이 레이저홍채절개술을, 16명이 수정체유화술을 시행 받았다. 술 후 1일째 시력(logMAR)에서는 레이저홍채절개술군이 0.62 ± 0.51로 유의하게 높았으며(p=0.048) 6개월째에는 수정체유화술군이 0.07 ± 0.15로 통계적으로 유의하게 높았지만(p=0.013) 1년째 경과 관찰 시에는 두 군 간의 유의한 차이는 보이지 않았다. 술 후 안압의 경우 1달째에 수정체유화술군의 안압이 9.5 ± 1.3 mmHg로 낮게 관찰되었으나(p=0.032) 이외의 관찰 기간에서 통계적으로 유의한 차이는 보이지 않았다. 술 후 1년째의 안압 하강제의 사용 개수는 레이저홍채절개술군 0.7 ± 0.4개, 수정체유화술군 0.4 ± 0.5개로 유의한 차이를 보였다(p=0.031). 안내염이나 시력상실 등의 심각한 합병증은 두 군에서 모두 관찰되지 않았다. 결론: 급성폐쇄각녹내장의 초기 치료로서 초기 수정체 유화술은 레이저홍채절개술에 비해 술 후 시력 및 안압, 그리고 합병증 발생에 있어 유의한 차이를 보이지 않았으며, 술 후 1년째의 안압 하강제의 사용 개수가 적었다. Purpose: We compared the outcomes of laser iridotomy and primary phacoemulsification when treating acute angle-closure glaucoma. Methods: This study was conducted with 61 patients diagnosed with acute angle closure glaucoma from January, 2005 to January, 2015. The patients received either laser iridotomy or primary phacoemulsification. The age and gender of each patient, differences of intraocular pressure (IOP) before and after treatment, visual acuity, need for IOP lowering agents and complications were analyzed retrospectively at 1-day, 1-month, 6-month and 12-month after the treatment. Patients who received laser iridotomy with phacoemulsification or trabeculectomy were excluded from this study. Additionally, we included only cases in which treatments were given within 3 days after the onset of symptom. Results: Among the 61 patients, 45 patients received laser iridotomy and 16 patients received primary phacoemulsification. One day after the treatment the laser iridotomy group showed better outcome in their visual acuity (log MAR 0.62 ± 0.51; p = 0.048). At 6-month postoperatively, the primary phacoemulsification group showed better visual acuity (log MAR 0.07 ± 0.15; p = 0.013). However, at 12-month postoperatively, the visual acuities were not significantly different between the 2 groups. Regarding IOP, at postoperative 1-month, the group of primary phacoemulsification shows significantly lower IOP (9.5 ± 1.3 mm Hg), compared with the group of laser iridotomy. A significant difference was observed in the number of IOP lowering agents that patients at 12 month after the treatment. There were no severe complications in either group. Conclusions: Following the initial treatment of acute angle-closure glaucoma, the primary phacoemulsification showed no significant differences in postoperative visual acuity, IOP and complications compared to the outcomes of laser iridotomy. Additionally, the need for IOP lowering agents was less in the primary phacoemulsification groups at 1 year after the operation.