금은화, 연교, 포공영 혼합물의 항염증 작용에 관한 연구

최강민 ( Kang Min Choi ),전주현 ( Ju Hyun Jeon ),김은석 ( Eun Seok Kim ),성기정 ( Ki Jung Sung ),김영일 ( Young Il Kim ) 대전대학교 한의학연구소 2021 혜화의학회지 Vol.30 No.1

Objective : The purpose of this study is to investigate the inflammatory-control effects of Cheonghyeol-antidote complex(Lonicera japonica Thunberg, Forsythia viridissima Lindley, and Taraxacum platycarpum H. Dahlstedt complex, CHA) in LPS-induced RAW264.7 cell and mouse inflammation models. Method : For in vitro and in vivo experiment, Indicators such as cell viability, mRNA expression level(iNOS, IL-6, IL-1β, COX-2, TNF-a), Inflammatory factor production(NO, IL-6, IL-1β, TNF-a), and protein phosphorylation level(ERK, JNK, p38) were analyzed. For in vivo experiment, Indicators such as mRNA expression level(iNOS, IL-6, IL-1β, COX-2, TNF-a), Inflammatory factor production(IL-6, IL-1β, TNF-a), protein phosphorylation level(ERK, JNK, p38) and immune cell(white blood cell, lymphocyte) were analyzed. Results : 1. In vitro experiment In cell viability of CHA, CHA showed cell viability below 90% at concentrations of 400 μg / ml or more. In mRNA expression level, IL-6 and IL-1β showed a significant decrease at all concentrations except 25 μg / ml concentration, and iNOS, COX-2, and TNF-a showed a significant decrease at all concentrations of CHA compared to the control group. In inflammatory factor production, NO and TNF-a showed a significant decrease at all concentrations except 25 μg / ml concentration of CHA, and IL-1β showed a significant decrease at 100, 200 μg / ml concentration of CHA compared to the control group. IL-6 showed a significant decrease at all concentration of CHA compared to the control group. In protein phosphorylation level, ERK and p38 showed a significant decrease at all concentrations except 25 μg / ml concentration of CHA and JNK showed a significant decrease at all concentrations of CHA compared to control group. 2. In vivo experiment In mRNA expression level, iNOS, COX-2 and TNF-a showed a significant decrease in all administration groups of CHA compared to the control group. In Inflammatory factor production, IL-6, IL-1β and TNF-a showed a significant decrease in all the administration groups of CHA. In protein phosphorylation level, ERK, JNK, and p38 showed a significant decrease in all the administration groups of CHA. In the immune cells, leukocytes and lymphocytes showed a significant decrease in all the administration groups of CHA. Conclusions : This study shows that CHA has antioxidant and inflammatory-control effects on LPS-induced RAW264.7 cells. It is hoped that further research will be conducted on the individual mechanisms of Lonicera japonica Thunberg, Forsythia viridissima Lindley, and Taraxacum platycarpum H. Dahlstedt.

계작지모가우슬탕(桂芍知母加牛膝湯) 약침이 류마티스 관절염 생쥐에 미치는 영향

정순현 ( Soon Hyun Jung ),조종관 ( Chong Kwan Cho ),김소연 ( So Yun Kim ),김영일 ( Young Il Kim ) 대전대학교 한의학연구소 2016 혜화의학회지 Vol.24 No.2

Objectives : The purpose of this study is to prove the effect and mechanism of Gamikyejakjimogawusul-tang(GKHA) herbal acupuncture on induced rheumatoid arthritis model of DBA/1 mice. Methods : We check effect of GKHA extract on the AST, ALT, Creatinine, BUN of serum and cell viability of GK extract in RAW 264.7 cells to test the stability of this study. In vitro, we measure total phenol contents, total flavonoid contents, DPPH free radical scavenging activity, ABTS cation radical scavenging activity of Gamikyejakjimogawusul-tang, effect of GK extract on ROS(Reactive Ooxygen Species) production to estimate a anti-oxidant capacity, and we also measure effect of GK extract on NO (Nitric Oxid), IL-1β, IL-6, IL-17, IL-21, TNF-α, MCP-1, GM-CSF production in RAW 264.7 cells to estimate a anti-inflammatory efficacy. In vivo, we compare a rheumatoid arthritis manifestation between control and experimental group and estimate a AI. Then we check effect of GKHA on the level of WBC, neutrophil, lympocyte, monocyte in the blood to see the effect of immune cells in blood. In addition we measure effect of GKHA on the level of hs-CRP, IgM, IgG, IL-1β, IL-6, IL-17, IL-21, TNF-α, MCP-1, GM-CSF in serum. We observe effects of GKHA on imaging of cartilage degeneration using micro CT-arthrography in paw hind. And we calculate effects of GKHA that reduced BV ratio, BS/BV ratio using 3D Micro-CT. Lastly we observe effects of GKHA histopathologic examination analysis. Results : 1. The toxicity on liver and kidney was disregardable and the cytotoxicity against RAW 264.7 cells was also disregardable. < In vitro > 1. Total phenol contents and total flavonoid contents in GK extract were in high level. 2. DPPH free radical scavenging activity and ABTS cation radical scavenging activity were increased according to concentration of GK extract 3. ROS production was significantly decreased in GK extract (at 10, 100 ㎍/ml). 4. NO, IL-6, TNF-α, MCP-1 production were significantly decreased in GK extract(at 10, 100 ㎍/ml). IL-17, GM-CSF production were significantly decreased in GK extract(at 1, 10, 100 ㎍/ml). IL-1β, IL-21 production were also decreased but there was no statistical significance. 5. 25x observation after H&E and M-T staining, infiltration of immune cells and subsidence of the cartilage and damage to the synovial cells were decreased. Conclusions : This study showed that GKHA extract had anti-oxidant capacity, anti-inflammatory efficacy. GKHA extract also had inhibiting effect on the process of rheumatoid arthritis and can protect joint and cartilage. So we expect that GKHA extract can be a meaningful treatment to rheumatoid arthritis patients.

족관절염좌(足關節捻挫) 환자(患者) 26례(例)에 대(對)한 체침(體鍼) 치료(治療)와 Trigger Point 치료(治療)와의 비교(比較) 고찰(考察)

김영일,김영화,이현,이병렬,Kim, Young-Il,Kim, Young-Hwa,Lee, Hyen,Lee, Byung Ryul 대한침구의학회 2001 대한침구의학회지 Vol.18 No.5

Objective : This study is to compare acupuncture treatment group with Trigger point treatment group about Ankle sprain treatment Methods : From March 1th 2001 to May 31th 2001, the Clinical comparison studies were carried out 26 cases of patient with Ankle sprain with acupuncture treatment group and, who had been treated in the Dept. of Acupuncture and Moxibustion, Taejon Oriental Medical Hospital, Taejon University. Results : 1. About period of treatment : 0-1week was the most number 9 cases, 1-2weeks were 3 case 2-3weeks were 1 case in the Acupuncture treatment group, and 0-1weeks were the most number 11 cases, 1-2weeks were 2 cases in the Trigger point treatment group. 2. About effect of treatment : Excellent were 7 cases, Good 3 cases, Fair 3 cases in the Acupuncture treatment group, and Excellent were 8 cases, Good 4 cases, Fair 1 case in the Trigger point treatment group. 3. About effect of treatment in the distribution of grade in descending order : As a genial rule, GrIII of 1 case changed to 0 case, Gr II 2 cases to 0 case, Gr I 10 cases to 3 cases in the Acupuncture treatment group, and GrIII of 1 cases changed to 0 cases, GrII 3 cases to 0 case, Gr I9 cases to 1 case in the Trigger point treatment group. Conclusion : The Trigger point treatment group is more effective than the Acupuncture treatment group.

백서(白鼠)의 족삼리(足三里)에 시술한 청풍등약침(靑風藤藥鍼)이 항암(抗癌) 및 면역증강(免疫增强)에 미치는 영향

김영일,홍권의,임윤경,이현,이병렬,김태한,Kim, Young-Il,Hong, Kwon-Eui,Yim, Yun-Kyoung,Lee, Hyeon,Lee, Byung-Ryul,Kim, Tae-Han 경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.1

Objectives and methods : To study on the anti-cancer, anti-metastasis and immune response improvement effects of Herbal-acupuncture with Sinomenii acuti Lignum infusion solution(SAL-HAS), we injected Sinomenii acuti Lignum infusion solution into Joksamni$(ST_{36})$ of C57BL/6 mouse which is corresponding to human Joksamni(ST36). We observed its effect on the number of $CD25^+/CD4^+,\;CD8^+/CD3e^+,\;CD69^+/B220^+,\;NK1.1^+/CD3e^+$ cells in mouse PBMCs(peripheral blood mononuclear cells), the number of the pulmonary colony, and the effect on MST(mean survival time) and ILS(increase in MST over control) of C57BL/6 mice implanted intravenously with B16-F10 melanoma. Results and Conclusions : 1. The spleen cells proliferation of the sample groups treated with SAL-HAS extract has increased significantly compared with that of the control group. 2. The percentage of the $CD25^+/CD4^+,\;CD8^+/CD3e^+,\;CD69^+/B220^+,\;NK1.1^+/CD3e^+$ cells in C57BL/6 mouse PBMCs of the sample groups treated with SAL-HAS has increased compared with that of the control group. 3. The pulmonary colony number of the sample groups SAL-HAS has decreased significantly compared with that of the control group. 4. MST and ILS of the sample groups SAL-HAS have increased significantly compared with those of the control group.

족삼리(足三里)(St36)의 전침자극(電鍼刺戟)이 fMRI상 뇌활성(腦活性) 변화(變化)에 미치는 영향(影響)

김영일,김영화,임윤경,이현,이병렬,김연진,Kim, Young-il,Kim, Young-hwa,Lim, Yun-kyoung,Lee, Hyen,Lee, Byung-ryul,Kim, Yeon-jin 대한침구의학회 2003 대한침구의학회지 Vol.20 No.5

Objective: Recently. many studies have showed the evidences of the effect of the Electro-acupuncture treatment through scientific. Methods : One of these methods is functional MRI. We performed electro-acupuncture on Zusanli(St36) and observed the change of brain activation using fMRI. Zusanli(St36) is located on the lateral side of the lower leg. 3 cun(寸) inferior to the Patella of the lower border. Theoretically and clinically. this point has been considered very important for gynecological disorders. spleen and stomach disorders. and psychological disorders. To see the effects of electro-acupuncture stimulation on Zusanli(St36). the experiment was carried out on twelve healthy volunteers. using the gradient echo sequence with the 3.0T whole-body fMRI system(ISOL). After the needle insertion on right Zusanli(St36). 2 Hz of electric stimulation was given for 30 seconds. repeated five times. with 30 seconds' intervals. The Image analysis including motion correction. talairach transformation, and smoothing was done with SPM99. Results ad conclusion : The electro-acupuncture stimulation on Zusanli(St36) activates Brodmann Area 6, 13, 2, 19, 21, 22, 39, 40, 38, 3which indicates the pathways of the electro-acupuncture stimulation on Zusanli(St36) and the possibility of the relationship of the electro-acupuncture stimulation on Zusanli(St36) with autonomic nervous system, internal organic system.

원격검침 설비의 네트워크 상태감시를 위한 SNMP 기반의 저압 AMI 망관리시스템 개발

김영일,박소정,김영준,정남준,최문석,박병석,Kim, Young-Il,Park, So-Jeong,Kim, Young-Jun,Jung, Nam-Jun,Choi, Moon-Suk,Park, Byung-Seok 한국전력공사 2016 KEPCO Journal on electric power and energy Vol.2 No.2

KEPCO installed AMI (Advanced Metering Infrastructure) metering system for low-voltage customers from 2008. AMI metering system of KEPCO has operated 2.55 million customers and will plan to operate 22 million customers until 2020. KEPCO developed AMI NMS (Network Management System) to operate the meter reading network efficiently. NMS monitors the network status of DCUs (Data Concentration Unit) and modems. NMS provides functionalities of data collection and analysis. It collects property data of network device, network topology information, communication performance information, fault information, and etc. It analyzes collected data and controls network devices by remote access. AMI NMS collects about 370 MIBs (Mangement Information Bases) using SNMP (Simple Network Management Protocol). This paper introduces main functionalities, designed context, and implemented service screen. 한국전력공사는 200년부터 저압 고객에 대한 AMI (Advanced Metering Infrastructure) 기반의 검침시스템을 구축하여 운영하고 있다. 한전의 AMI 검침시스템은 현재 255만호에 설치되어 운영되고 있으며 2020년까지 2200만호를 설치하여 운영할 예정이다. 본 연구에서는 원활한 AMI 검침망 운영을 위해 DCU (Data Concentration Unit) 및 모뎀에 대한 네트워크 동작상태를 감시할 수 있는 AMI 망관리시스템을 개발하였다. AMI 망관리시스템은 네트워크 장비에 대한 속성 데이터 및 네트워크 토플로지 정보, 통신성능 정보, 장애정보 등을 수집하고, 데이터 분석을 통해 장애내역을 생성하며, 원격에서 장비를 제어하는 등의 기능을 제공하고 있다. AMI 망관리시스템은 전력선 통신망에서 네트워크 장비에 대한 약 370여개의 MIB (Management Information Base) 정보를 SNMP (Simple Network Management Protocol)를 이용하여 수집과 제어를 수행하고 있다. 본 논문에서는 AMI 망관리시스템에 대한 주요 기능과 설계내용, 구현된 서비스 화면을 소개하고자 한다.

목단피((牧丹皮).도인(桃仁) 배합(配合)이 항염증(抗炎症) 작용(作用)에 미치는 영향

김영일,이성준,허진,이태형,신동근,이재철,신용서,윤용갑,Kim, Young-Il,Lee, Sung-Jun,Huh, Jin,Lee, Tae-Hyung,Shin, Dong-Gean,Lee, Jae-Cheol,Shin, Yong-Seo,Yun, Young-Gab 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.1

Paeonia Suffruticosa and Prunus Persica have been used as oriental medicine for removal of fever, alleviation of pain, an anti-phlogistic effect and removal of extravasated blood. However, it has been never shown the effects of these herbal medicines on anti-inflammatory processes. This experiment was performed to show how these herbs could act as anti-inflammatory medicines at cellular level. Anti-inflammation effects of water extracts from Paeonia Suffruticosa and Prunus Persica as well as their mixture have been investigated, and the results were follows; 1) each extract slightly suppressed the expression and production of inflammatory mediators and enzymes such as NO, iNOS, IL-$1{\beta}$, and TNF-$\alpha$ in lipopolysaccharid(LPS)-stimulated RAW264.7 cells and mouse primary peritoneal macrophages in a dose-dependent manner. These suppressive effects, however, were synergistically increased by their mixture. 2) Each extract of Paeonia Suffruticosa and Prunus Persica insignificantly suppressed the activation and activity of NF-${\kappa}B$ in LPS-stimulated RAW264.7 cells, which controls the expression of inflammatory mediators such as NO, iNOS, IL-$1{\beta}$, and TNF-$\alpha$. However, extract mixture of Paeonia Suffruticosa and Prunus Persica suppressed effectively the activation and activity of NF-${\kappa}B$. 3) Each of Paeonia Suffruticosa and Prunus Persica induced translocation of NF-${\kappa}B$ to the nucleus from the cytosol and DNA-binding activity of nuclear NF-${\kappa}B$ in LPS-activated RAW264.7 cells. The extract mixture of Paeonia Suffruticosa and Prunus Persica showed more significant suppression of the NF-${\kappa}B$ translocation and its DNA-binding activity, as compared to those of the each extract. These results suggest that the extract mixture of Paeonia Suffruticosa and Prunus Persica may affect different control mechanisms for NF-${\kappa}B$ activation and the expression and production of NF-${\kappa}B$-dependent inflammatory mediators, indicating that this extract mixture may be useful for treatment of inflammatory diseases.

열적 제한요소를 고려한 열회수 증기발생기의 시동 특성 해석

김영일,김동섭,김재환,노승락,고상근,Kim, Young Il,Kim, Tong Seop,Kim, Jae Hwan,Ro, Sung Tack,Kauh, Sang Ken 대한기계학회 1999 大韓機械學會論文集B Vol.23 No.11

A thorough understanding of the transient behavior during start-up is essential in the design and operation of the heat recovery steam generator(HRSG). During this period of time, material that is exposed to high temperature and experiences a large temperature variation is subject to high thermal stress. In this work, a transient formulation of the HRSG is constructed including the estimation of the thermal stress and fatigue of the drum wall. Start-up behavior of a single-pressure HRSG is analyzed and the effect of bypassing part of the gas turbine exhaust flow on the thermal stress evolution is examined. It is found that the modulation of the gas flow rate using a bypass damper is very useful in view of reducing the thermal stress of the drum and ensuring the fatigue lifetime.

세신약침(細辛藥鍼)의 천식억제 및 면역조절효과에 대한 실험적 연구

김일구 ( Il Gu Kim ),김영일 ( Young Il Kim ),홍권의 ( Kwon Eui Hong ),임윤경 ( Yun Kyoung Yim ),이현,이병렬 ( Byung Ryul Lee ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.1

Objectives and methods: The aim of this study was to investigate the effect of AHCR(ASARI HERBA CUM RADICE) herbal acupuncture at St36 on ovalbumin-induced asthma in mice. C57BL/6 mice were sensitized and challenged with OVA(ovalbumin) for 12 weeks(once a week). Two experimental groups were treated with different concentrations(1%, 0.1%) of AHCR-HAS at Chok-samni (St36) for the later 8 weeks (3times/week). Results: 1. Lung weight of the mice group treated with AHCR-HA decreased significantly compared with that of control group. 2. Total Leukocytes in BALF of the mice group treated wtih AHCR-HA decreased significantly compared with those of control group. 3. The number of Eosinophils in BALF of the mice group treated wtih AHCR-HA decreased significantly compared with that of control group. 4. The number of Gr-1+ /CD11b+, CCR3+, CD4+, CD3e+/CD69+, IgE+/B220+ cells in the lungs of the mice group treated with AHCR-HA decreased significantly compared with that of control group. 5. The number of CD8+ cells in the lungs of the mice group treated with AHCR-HA didn`t show significant difference compared with that of control group. 6. The concentration of IgE, IL-13, IL-4 in serum of the mice group treated with AHCR-HA decreased significantly compared with that of control group. Conclusion: We conclude that AHCR-HA is effective on OVA-induced asthma in C57BL/6 mouse.

족삼리(足三里) 독활약침(獨活藥鍼)이 Collagen-induced Arthritis에 미치는 영향

양기영,김영일,이현,Yang, Gi-Young,Kim, Young-Il,Lee, Hyeon 대한침구의학회 2006 대한침구의학회지 Vol.11 No.2

Objective & Methods : The purpose of this study is to observe the effects of Angelicae Pubescentis Radix herbal-acupuncture solution(APR-HAS) at Joksamni(ST36) on collagen IT induced arthritis in DBA/1J mice. The author performed several experimental items to analyze several cytokines and immune cells related with RA. Results : 1. In the APR-HA group, the incidence of arthritis and arthritis index were significantly decreased. 2. In APR-HA group, the levels of IL-6, $INF-{\gamma}$, $TNF-{\alpha}$, IgG, IgM, $IL-{\beta}$ and Anti-collagen II in serum of the CIA mouse were significantly decreased. 3. In APR-HA group, the level of $IFN-{\gamma}$, IL-4 in the CIA mouse spleen cell culture were significantly decreased. 4. In histology, the cartilage destruction and synovial cell proliferation were decreased in the APR-HA group, and the collagen fiber expressions in the APR-HA group were similar with that of the Normal group. 5. In the APR-HA group, CD3e+/CD19+ and CD4+/CD8+ were similarly maintained as Normal group in the CIA mouse lymph nodes, 6. In the APR-HA group, CD3e+/CD69+ was significantly decreased in the CIA mouse joint. 7. In the APR-HA group, CD11a+/CD19+ and CD11b+/Gr-l+ were significantly decreased in the CIA mouse lymph nodes 8. In the APR-HA group, CD4+/CD25+ was decreased in the CIA mouse spleen cell. 9. In the APR-HA group, CD4+/CD25+ was similarly maintained as Normal group in the CIA mouse lymph nodes.