큰열매모자반(Sargassum macrocarpum) 추출물의 항산화 효과 및 세포 활성 효과

김숙희,Kim, Sook-hee 한국융합학회 2021 한국융합학회논문지 Vol.12 No.8

본 연구에서는 큰열매모자반 추출물의 식품 및 화장품으로서 이용성을 평가하기 위해 항산화능 및 항염능, 항비만효과를 확인하였다. 항산화실험으로서 폴리페놀, 플라보노이드, DPPH, ABTS, NO, FRAP을 실시하였다. 폴리페놀의 경우 30.81±1.12 mg/g으로 나타났다. 플라보노이드의 경우 25.72±0.94 mg/g으로 나타났다. DPPH 실험에서는 6.746 mg ascorbic acid / g extract의 항산화능을 나타내었으며, ABTS 실험에서는 15.59 mg ascorbic acid / g extract의 항산화능을 나타내었으며, NO 실험에서는 6.781 mg ascorbic acid / g extract의 항산화능을 나타내었다. FRAP에서는 큰열매모자반 추출물의 1 mg이 ascorbic acid 4.573±0.097 ㎍의 환원력을 보였다. 한편 세포실험에서는 큰열매모자반 추출물이 세포독성이 없음을 보였으며, NO 생성 억제능의 경우 25.95±0.85%의 염증 억제능을 보였으며, 29.75±2.35%의 지질 축적 억제능을 보여 큰열매모자반 추출물이 항염능, 항비만능을 가진 이너뷰티 제품 원료로서 사용가능함을 보였다. 향후 연구에서는 큰열매모자반이 포함하는 단일 물질들이 항산화, 항염, 항비만에 어떠한 기전으로 영향을 미치는지 연구할 필요가 있다. In this study, the antioxidant and anti-inflammatory, anti-obesity properties, of Sargassum macrocarpum extracts were identified to assess the availability of Sargassum macrocarpum extracts as cosmetics and foods. To measure antioxidant activity, we conducted TPC, TFC, DPPH, ABTS, NO, FRAP. For polyphenols, 30.81±1.12 mg/g was shown. Flavonoids showed 25.72±0.94 mg/g. The DPPH experiment showed an antioxidant function of 6.746 mg ascorbic acid/g extract, the ABTS experiment showed an antioxidant function of 15.59 mg ascorbic acid/g extract, and the NO experiment showed an antioxidant function of 6.781 mg ascorbic acid/g extract. In FRAP, 1 mg of the Sargassum macrocarpum extract showed a reduction of 4.573±0.097 ㎍ of ascorbic acid. In cytotoxicity experiments, Sargassum macrocarpum extracts showed a cell survival rate of more than 80% at all concentrations, and an inflammatory inhibition of 25.95±0.85%, and an lipid accumulation inhibition of 29.75±2.35%. These results indicate that Sargassum macrocarpum extract is available as an anti-inflammatory cosmetic and anti-obesity inner beauty material. In future studies, it is necessary to study how pure substances containing Sargassum macrocarpum extract affect antioxidants, anti-inflammatory and anti-obesity

소비자의 신선육 구매실태 조사

김숙희,이무하 ( Sook Hee Kim,Moo Ha Lee ) 한국축산학회 1986 한국축산학회지 Vol.28 No.2

In order to help fresh meat processing industry, a survey on consumer`s purchasing pattern of fresh meat was carried out in the area of apartments and houses in Seoul. A total of 1,000 housewives was interviewed with questionnaires. 1. Among meat and poultry, beef was the first preference, pork was the text and the third was poultry. With the increase of income, the preference for beef increased-as the income increased by 100,000 won, the increased amount of purchase was 300g for beef, 90g for pork and 0.1 head for poultry while with 10,000 won increase of groceries expense, purchase of beef, pork and poultry increased by 1608, 40g 0.04 head respectively. Accordingly, measures to boost the preference for pork and poultry should be taken to reduce the relative preference of high-income group for beef. 2. Because 40% of participants, the highest proportion said they would buy more meat if fresh meat in sold as graded and prepackaged forms with different prices, sales volume would increase if fresh meat industry adops the grading and prepackaging system. The meat color was the main criterion of consumer`s selection of meat at retail shops. Therefore, it is important for fresh meat processing industry to watch freshness of meat, selection of packaging films and temperature control at retail shops carefully to maintain meat color red.

논문편 - 식생활 분야 : 식이내 단백질 제한과 회복이 흰쥐의 성장에 미치는 영향

김숙희,이귀세라 ( Sook Hee Kim,Gui Se Ra Lee ) 대한가정학회 1982 Family and Environment Research Vol.20 No.1

쌍발이모자반(Sargassum patens) 추출물의 항산화 및 항염효과

김숙희,Kim, Sook-hee 중소기업융합학회 2021 융합정보논문지 Vol.11 No.7

In this study, the antioxidant and anti-inflammatory properties of Sargassum patens extracts were identified. Antioxidant experiments included polyphenol concentration measurements, flavonoid concentration measurements, DPPH experiments, ABTS experiment NO experiments, and FRAP experiments. For polyphenols, 18.99±0.69 mg/g was shown. Flavonoids showed 11.89±1.16 mg/g. The DPPH experiment showed an antioxidant function of 19.78 mg ascorbic acid/g extract, the ABTS experiment showed an antioxidant function of 63.64 mg ascorbic acid/g extract, and the NO experiment showed an antioxidant function of 7.966 mg ascorbic acid/g extract. In FRAP, 1 mg of the moxibustion extract showed a reduction of 2.089 ㎍ of ascorbic acid. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions against inflammation induced by LPS. In cytotoxicity experiments, Sargassum patens extracts showed a cell survival rate of more than 80% at all concentrations, and an inflammatory inhibition of 30.64±0.23% at a concentration of 100 ㎍/mL. These results indicate that Sargassum patens extract is available as an anti-inflammatory cosmetic material. 본 연구에서는 쌍발이모자반 추출물의 항산화능 및 항염능을 확인하였다. 항산화능 실험에는 폴리페놀 농도측정, 플라보노이드 농도 측정, DPPH 실험, ABTS 실험, NO 실험, FRAP 실험을 실시하였다. 폴리페놀의 경우 18.99±0.69 mg/g으로 나타났다. 플라보노이드의 경우 11.89±1.16 mg/g으로 나타났다. DPPH 실험에서는 19.78 mg ascorbic acid / g extract의 항산화능을 나타내었으며, ABTS 실험에서는 63.64 mg ascorbic acid / g extract의 항산화능을 나타내었으며, NO 실험에서는 7.966 mg ascorbic acid / g extract의 항산화능을 나타내었다. FRAP에서는 쌍발이모자반 추출물의 1 mg이 ascorbic acid 2.089 ㎍의 환원력을 보였다. 한편 세포실험에서는 세포 독성과 LPS로 유도된 염증에 대한 항염능을 알아보았다. 세포독성의 경우 모든 농도에서 80%이상의 세포 생존률을 보였으며, NO 생성 억제능의 경우 100 ㎍/mL 농도에서 30.64±0.23%의 염증 억제능을 보여 쌍발이모자반 추출물이 항염능을 가진 화장품 원료로서 사용가능함을 보였다.

N-6 , N-3 지방산과 비타민 E 보강 식이가 전암성 병변에 미치는 영향

김숙희,최혜미 ( Sook Hee Kim,Hay Mie Choi ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.2

Weaning Sprague Dawley male rats were fed on com oil and perilla oil at 15l (w/w) of diet supplemented with vitamin E at 100 and 1,000IU/㎏ diet for 10 weeks. Hepatocellular carcinogenesis was induced by Solt & Farber modification (200 ㎎ diethylnitrosamine, 3 week feeding of 0.021 of 2-acetylaminofluorene, and partial hepatectomy). Placental glutathione S-transferase(GST-P) positive foci, the activities of glutathione S-transferase (GST), and glucose 6-phosphatase(G6Pase) and lipid peroxide values were measured. Lipid peroxide values in the perilla oil groups, in spite of its high degree of fatty acid unsaturation, were lower than expected and this trend was the same with 1,000IU vitanmin E compared to 100IU vitamin E. By perilla oil and 1,OOOIU vitamin E diet, lipid peroxide seemed to be eliminated by GST rather than to attact microsomal membrane. Perilla oil were seemed to reduce the area of placental GST-P positive foci more than com oil. The effect of vitamin E supplement on lipid peroxide was noticed only in com oil groups. This study suggests that not only degree of generation of lipid peroxide but also the effect of vitamin E supplement depend on the composition of fatty acid.

급식유형에 따른 중 · 고등학생의 학교급식 만족도

김숙희(Kim Sook Hee),이경애(Lee Kyoung Ae),유춘희(Yu Choon Hie),송요숙(Song Yo Sook),김우경(Kim Woo Kyung),윤혜려(Yoon Hei Ryeo),김주현(Kim Ju Hyeon),이정숙(Lee Jung Sug),김미강(Kim Mi Kang) 韓國營養學會 2003 Journal of Nutrition and Health Vol.36 No.2

밀버섯의 항암성분에 관한 연구

김숙희(Sook Hee Kim),김진숙(Jin Sook Kim),진미림(Mi Rim Jin),김하원(Ha Won Kim),최응칠(Eung Chil Choi),김병각(Byong Kak Kim) 한국생약학회 1994 생약학회지 Vol.24 No.4

To find antitumor components from higher fungi, the mycelia of Collybia confluens (Pers. ex Fr.) Kummer were cultured in artificial media. For efficient production of the mycelia, the influences of various modifications of culture conditions were examined. A water-soluble protein-bound polysaccharide fraction, Fr. A, was obtained from the mycelia by hot water extraction. When Fr. A was purified and fractionated by DEAE-cellulose and Sephadex G-200 gel filtration chromatographies into four fractions which were designated B, C, C-I and C-II. The tumor inhibition ratios of these fractions ranged from 46% to 75%, against the solid forms of sarcoma 180 in ICR mice at doses of 20 and 50 ㎎/㎏/day when given intraperitoneally. Especially, Fr. C which was named Collyban(CB) exhibited a marked life-prolonging effect of the mice against ascitic forms of sarcoma 180 at a dose of 50 ㎎ via i.p. administration. To extend spectra of the antitumor activities and eliminate the effects of allograft rejection, the characterization of antitumor effects of CB was performed in syngeneic host-tumor systems. It did not show any antitumor activity against L1210 murine leukemia in CD2F1 mice but prolonged their life span against ascitic forms of MM46 carcinoma in C3H/He mice. Also it exhibited antitumor activity against human cervical cancer HeLa cells that were xenografted into nude mice having BALB/c genetic backgrounds by the i.p. injection at a dose of 100 ㎎/㎏/day. In order to characterize the antitumor components, CB was examined by chemical analysis. It was acidic protein-bound polysaccharides composed of 31% polysaccharide, 27% protein and 3% hexosamine. CB was fractionated into two fractions, Fr. C-I(M.W.: 500 Kd) and Fr. C-II (M. W.: 30 and 8 Kd) by Sephadex G-200 gel filtration chromatography.

비경쟁적 집단 활동이 유아와 교사가 인식하는 유아의 친사회적 행동에 미치는 영향

김숙희(Kim sook hee),송수희(Song soo hee) 한국포괄영유아아동교육지원학회 2013 포괄영유아,아동교육지원연구 Vol.1 No.2

본 연구는 비경쟁적 집단 활동이 유아와 교사가 인식하는 유아의 친사회적 행동에 미치는 영향에 대하여 알아보고자 하는데 그 목적이 있다. 본 연구의 대상은 만 4세 36명이였으며 자료 분석은 t-test를 사용하였다. 본 연구의 결과는 다음과 같다. 첫째, 비경쟁적 집단 활동은 유아가 스스로 인식한 유아의 친사회적 행동과 정적인 상관관계가 나타났다. 둘째, 비경쟁적 집단 활동은 교사가 인식한 유아의 친사회적 행동에 정적인 상관관계가 나타났다. 셋째, 비경쟁적 집단 활동은 유아가 인식한 친사회적 행동의 하위영역에서는 개인정서조절 능력과 교육기관 적응능력에서 높게 나타났으며 교사가 인식한 친사회적 행동의 하위영역에서는 지도성, 도움주기, 의사소통, 주도적 배려, 접근시도, 나누기, 감정이입 및 조절에서 유의미하게 나타났다. This study has the purpose to examine the effect of non-competitive group play activities on the prosocial behaviors of young children recognized by themselves and the prosocial behaviors of young children recognized by teachers Of all 4-year-old children in Y kindergarten, Chuncheon City, I selected 17 young children in Class A as an experimental group and 19 young children in Class B as a comparative group for determining the objects of this study. The procedures of this study were composed of preliminary exam, pretest, experiments for 14 weeks and post-test, and I conducted the experiments 26 times for 14 weeks from May. 02, 2011 to Aug. 24, 2011. The results of this study are as follows. First, there is a significant difference in the prosocial behaviors of young children recognized by themselves with respect to the non-competitive group play activities. As examining sub-factors, there is a significant difference in personal emotional regulation competence and adaptability in the kindergarten, but there is no significant difference in interpersonal relationship formative competence. Second, the non-competitive group play activities have an effect on the prosocial behaviors of young children recognized by teachers. These all have an effect on the leadership, offering help, communication, attempting access, initiative consideration, sharing, empathy and emotional self-regulation. As a result of this study, the non-competitive group play activities positively affect the prosocial behaviors of young children Therefore, these can be usefully applied to the prosocial behaviors and formulation of self-concept on the ground.

비행청소년의 분노와 충동성 조절을 위한 게슈탈트 관계성 향상 프로그램 효과 예비 연구

김숙희 ( Kim¸ Sook Hee ),김정규 ( Jungkyu Kim ) 한국게슈탈트상담심리학회 2014 한국게슈탈트상담연구 Vol.4 No.2

본 연구에서는 비행청소년을 대상으로 게슈탈트 관계성 향상 프로그램이 비행청소년의 분노와 충동성 조절 및 자기개념에 미치는 효과를 검증하였다. 연구 대상은 법원의 보호처분 명령에 따라 수도권 소재 아동보호 치료시설에 위탁 수용 중인 비행청소년들로써, 실험집단과 통제집단에 각각 8명씩 무선 할당하여 배정하였다. 치료집단에는 게슈탈트 관계성 향상 프로그램을 회기 당 120분씩 주 2회 5주에 걸쳐 실시하였다. 프로그램의 효과성을 검증하기 위하여 충동성 척도는 Barratt Impulsive Scale Ⅱ를, 분노 척도는 Novaco Anger Scale을, 자아개념검사 척도는 성신자아개념 척도를 각각 사용하였다. 본 연구의 결과는 다음과 같다. 첫째, 게슈탈트 관계성 향상 프로그램에 참여한 실험집단 비행청소년들은 통제집단에 비해 충동성이 유의하게 감소하지 않았다. 둘째, 프로그램에 참여한 실험집단 비행청소년들은 분노수준이 유의하게 감소하지 않았다. 셋째, 프로그램에 참여한 실험집단 비행청소년들은 자기개념이 유의하게 상승하지 않았다. 결론적으로 본 연구에서 시행된 게슈탈트 관계성 향상 프로그램은 비행 청소년의 충동성과 분노, 자기개념에 영향을 주지 않았음을 알 수 있었다. 마지막으로 본 연구의 한계와 후속연구를 위한 제언을 하였다. The purpose of this study was to verify effectiveness of Gestalt Relationship Improvement Program for probationary juvenile delinquent's anger and impulsivity control and self-concept. The subjects were 16 female juvenile delinquents on probation who were detained in a children preservation facility. Eight of them were randomly divided into experimental group and control group each. During the experiment period, the experimental group was treated with group therapy sessions of 10 times, twice a week. Each session of the program lasted 110 minutes using Gestalt Relationship Improvement Program. To measure the degree of impulsivity, Barratt Impulsiveness Scale II was used. Novaco Anger Scale and Sungshin Self-concept Scale were also used to assess anger level and self-concept respectively. The result of this study shows that those who participated in the Gestalt Relationship Improvement Program did not make a significant reduction in impulsivity and anger, and they did not make a significant increase in self-concept, either. In conclusion, the Gestalt Relationship Improvement Program implemented in this study was not helpful to probationary juvenile delinquents in terms of their impulsivity, anger and self-concept. The implications and limitations of the study were discussed along with suggestions for further research.

사회복지사들의 직무특성과 조직몰입이 직무만족에 미치는 영향

김숙희 ( Sook Hee Kim ),박용억 ( Yong Euk Park ) 한국노인의료복지학회 2010 노인의료복지연구 Vol.2 No.1

This study investigates effects of organizational commitment on job satisfaction of social workers who work at elderly care welfare facilities in 8 different regions in Daegu-Gyeongbuk. 250 social workers participated for 2 months, from June 1st to July 30th, 2009. 1) As characteristics of individual job performance are analyzes by ANOVA with 5 points as Likert`s highest score, the average point is 3.84±0.55, showing high job performance. Variables with statistical significance are age, education, marital status, working place, monthly income, and health condition. 2) ‘Characteristics of job and role’ are an average of 3.83±0.65, showing high characteristics of job and role. Variables with statistical significance are sex, age, education, marital status, religion, working place, monthly income, and health condition. 3) Organizational characteristics are an average of 3.65±0.51. Variables with statistical significance are age, marital status, relation (p<0.05), working place, monthly income, and health condition. 1. 4) Job satisfaction is an average of 3.21±0.32, which is low. A variable with statistical significance is health condition. 5) Job commitment is an average of 3.45±0.49, which is low. Variables with statistical significance are age, marital status, religion, working place, monthly income, and health condition. 6) Organizational commitment is an average of 3.45±0.49. Variables with statistical significance are individual character, job and role character, and organizational character. 7) `Job satisfaction` by job character and organizational commitment is an average of 3.21±0.32. Variables with statistical significance are individual characher by job performance, job and role character, organizational character, and job commitment. 8) Among factors on job satisfaction, individual character of job performance has the biggest impact, followed by the level of job commitment, sex, health condition, and employment type.