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      • 주얼리 산업의 ESG경영 활동이 브랜드 이미지 및 브랜드 신뢰가 소비자 행동의도에 미치는 영향에 관한 연구=

        김선희 경기대학교 서비스경영전문대학원 2024 국내박사

        RANK : 247647

        최근 대중들에게도 관심인 ESG는 기업 경영전략의 핵심으로서 환경(E), 사회(S), 지배구조(G) 면에서 기업의 가치를 높이고 지속성장을 추구하는 기업의 필수적인 경영전략으로 대두되고 있다. 최근 기후 위기에 대응하는 정부의 환경정책과 ESG 정보공시 의무가 강화되면서 급속도로 변화하고 있는 반면에 주얼리산업 전반에서는 ESG경영 활동의 경쟁력이 매우 미비한 상황이다. ESG경영 활동은 다양한 부분과 많은 비용, 오랜 시간이 들어가는 실행하기 어려운 부분 중의 하나이겠지만 타 산업의 변화에 발맞춰 준비하는 것이 주얼리산업의 큰 과제이며, ESG경영 패러다임은 주얼리 산업군에서도 꼭 필요한 연구 대상으로 볼 수 있다. 본 연구는 주얼리 산업의 ESG경영 활동(환경, 사회, 지배구조)과 브랜드 이미지, 브랜드 신뢰, 소비자 행동의도(구매의도, 추천의도) 간의 영향 관계를 분석하는 데 목적이 있다. 이러한 목적을 달성하기 위하여 2023년 주얼리 및 ESG경영 활동에 관심이 있는 소비자를 조사대상자로 총 400부의 설문지를 배포 370부를 회수하여 불성실하게 응답한 28부를 제외한 총 342부 설문지를 통계분석에 사용하였다. 이러한 자료를 분석한 결과는 다음과 같다. 첫째, <가설 1>을 검정한 결과, ESG 활동 요인 중 환경요인은 브랜드 이미지에 긍정적인 영향을 미치지 않는 것으로 나타났으나, 사회요인과 지배구조요인은 긍정적인 영향을 미치는 것으로 나타났다. 둘째, <가설 2>를 검정한 결과, ESG 활동 요인 중 환경요인은 브랜드 신뢰에 긍정적인 영향을 미치지 않는 것으로 나타났으나, 사회요인과 지배구조요인은 긍정적인 영향을 미치는 것으로 나타났다. 셋째, <가설 3>을 검정한 결과, 브랜드 이미지는 브랜드 신뢰에 긍정적인 영향을 미치는 것으로 나타났다. 넷째, <가설 4>를 검정한 결과, 브랜드 이미지는 행동의도의 요인 중 구매의도 유의한 영향을 미치는 것으로 나타났고, 브랜드 이미지는 행동의도의 요인 중 추천의도에 유의한 영향을 미치는 것으로 나타났다. 다섯째, <가설 5>를 검정한 결과, 브랜드 신뢰는 행동의도의 요인 중 구매의도 유의한 영향을 미치는 것으로 나타났고, 브랜드 신뢰는 행동의도의 요인 중 추천의도에 유의한 영향을 미치는 것으로 나타났다. 이러한 분석 결과를 바탕으로 본 연구에서는 마케팅 관점에서 주얼리 기업의 지속성장의 가능성 측면에서 ESG경영에 대한 효율적인 이해를 돕고, ESG경영 전략이 향후 국내 주얼리 산업에서도 실현 가능성을 모색하는 데 도움을 주고자 한다. ESG, which has recently attracted public attention, is the core of corporate management strategy and is emerging as an essential management strategy for companies that increase corporate value in terms of environment (E), society (S), and governance (G) and pursue sustainable growth.. Recently, the government's environmental policy in response to the climate crisis and the obligation to disclose ESG information have been strengthened and are rapidly changing, while the competitiveness of ESG management activities in the jewelry industry as a whole is very weak. ESG management activities are one of the difficult parts to implement, requiring various aspects, a lot of cost, and a long time, but preparing to keep up with changes in other industries is a big task for the jewelry industry, and the ESG management paradigm is an essential research subject in the jewelry industry as well. can see. The purpose of this study is to analyze the influence relationship between ESG management activities (environment, society, governance) of the jewelry industry and brand image, brand trust, and consumer behavioral intention (purchase intention, recommendation intention). To achieve this purpose, a total of 400 questionnaires were distributed to consumers interested in jewelry and ESG management activities in 2023, and 370 questionnaires were collected. A total of 342 questionnaires, excluding 28 questionnaires that responded insincerely, were used for statistical analysis. The results of analyzing these data are as follows. First, as a result of testing <!-- Not Allowed Tag Filtered --><Hypothesis 1>, environmental factors among ESG activity factors were found to have no positive effect on brand image, but social factors and governance factors were found to have a positive effect. Second, as a result of testing <!-- Not Allowed Tag Filtered --><Hypothesis 2>, environmental factors among ESG activity factors were found to have no positive effect on brand trust, but social factors and governance factors were found to have a positive effect. Third, as a result of testing <!-- Not Allowed Tag Filtered --><Hypothesis 3>, brand image was found to have a positive effect on brand trust. Fourth, as a result of testing <!-- Not Allowed Tag Filtered --><Hypothesis 4>, brand image was found to have a significant influence on purchase intention among the factors of behavioral intention, and brand image had a significant influence on recommendation intention among the factors of behavioral intention. It was found that Fifth, as a result of testing <!-- Not Allowed Tag Filtered --><Hypothesis 5>, brand trust was found to have a significant influence on purchase intention among the factors of behavioral intention, and brand trust had a significant influence on recommendation intention among the factors of behavioral intention. It was found that Based on these analysis results, this study aims to provide an efficient understanding of ESG management in terms of the possibility of sustainable growth of jewelry companies from a marketing perspective and to help explore the feasibility of ESG management strategies in the domestic jewelry industry in the future.

      • 위기청소년의 가정환경과 심리행동특성연구 : Drop in center를 중심으로

        김선희 인하대학교 교육대학원 2012 국내석사

        RANK : 247647

        <국문초록> 위기청소년의 가정환경과 심리행동 특성 연구 - Drop in center를 중심으로- 2012년 2월 인하대학교 교육대학원 심리치료상담전공 金善熙 지 도 교 수 朴榮信 본 연구는 위기청소년의 가정환경과 심리행동 특성을 연구하는데 주된 목적이 있다. 이를 위해 서울, 인천 및 대전에 있는 청소년드롭인센터를 중심으로 위기청소년 가정의 구조적 환경, 부모 및 본인의 학업기대, 위기청소년의 가출에 대한 질적 분석을 하였다. 또한 위기청소년의 일탈행동과 관련된 심리특성을 질적으로 분석하였다. 질적 분석 외에 양적 분석으로서, 부모자녀관계와 위기청소년의 심리특성 및 행동특성에 대한 상관분석을 하였다. 본 연구를 위한 조사는 2010년 12월부터 2011년 5월까지 6개월 동안에 실시하였으며, 분석대상은 총 251명(남: 122명, 여: 129명)이었다. 측정도구는 박영신(2010)이 제작한 질문지를 사용하였다. 연구 결과는 다음과 같다. 첫째, 위기청소년 가정의 구조적 환경 중 부모의 학력은 고졸이, 직업은 판매서비스직이, 가정경제수준은 중류층이 가장 많았다. 부모 및 본인의 학업기대는 대졸이 가장 많았다. 위기청소년의 가출 평균은 13회였고, 가출의 대표적인 이유는 부모와의 갈등이었으며, 가출 뒤의 대표적 숙박 장소는 PC방이었다. 둘째, 위기청소년의 일탈행동과 관련된 심리특성을 질적으로 분석한 결과, 학교에 결석하는 주된 이유는 학교가 싫어서였으며, 학교를 자퇴하는 주된 이유는 학교에 다니기 싫어서였다. 성매매 아르바이트를 하는 주된 이유는 돈을 벌려고 이였으며, 성관계하는 주된 이유는 성적 욕구로 나타났다. 자살을 시도하는 주된 이유는 살기 싫어서였으며, 절도를 하는 주된 이유는 돈이 없어서였다. 본드나 신나를 흡입하는 주된 이유는 호기심이었다. 셋째, 부모자녀관계와 위기청소년의 심리특성과의 관계를 분석한 결과, 부모의 무관심은 위기청소년의 낮은 자기효능감과 밀접히 관련되었다. 부모와 갈등이 많을수록 위기청소년의 도덕적이탈이 높았으며, 부모가 무관심할수록 위기청소년의 스트레스 증상 및 우울이 높았다. 또한 부모자녀관계와 위기청소년의 행동특성과의 관계를 분석한 결과, 부모가 무관심할수록 위기청소년의 학업성취도가 낮았으며, 부모와 갈등이 많을수록 위기청소년들이 일탈행동을 많이 하였다. 주제어: 위기청소년, 부모자녀관계, 가출, 학교 자퇴, 성매매 아르바이트, 성관계, 자기효능감, 도덕적이탈, 스트레스, 일탈행동

      • 통합유치원 교사의 교직관, 교사효능감, 교직헌신도에 관한 연구 : -대구광역시를 중심으로-=

        김선희 대구대학교 2013 국내석사

        RANK : 247647

        This research aims to examine teaching profession ideologies, teacher efficacies, degree of devotions to teaching profession based on whether integrated kindergarten teachers completed integrated education related training and whether the courses are completed, and to find relations among teaching profession ideologies, teacher efficacies, and degree of devotions to teaching profession in detail. Research subjects set for these purposes are as followings. First, how is teaching profession ideologies related to personal backgrounds (whether integrated education related training and courses are completed) of integrated kindergarten teachers ? Second, how is teacher efficacies to teaching profession related to personal backgrounds (whether integrated education related training and courses are completed) of integrated kindergarten teachers ? Third, how is degree of devotions to teaching profession related to personal backgrounds (whether integrated education related training and courses are completed) of integrated kindergarten teachers ? Fourth, how are teaching profession ideologies and teacher efficacies of integrated kindergarten teachers related? Fifth, how are teacher efficacies and degree of devotions to teaching profession of integrated kindergarten teachers related? Sixth, how are teaching profession ideologies and degree of devotions to teaching profession of integrated kindergarten teachers related? Subjects of this research are 186 of public and private kindergarten teachers located in Daegu metropolitan city, where integrated education is implemented, and questionnaire was used to measure teaching profession ideologies, teacher efficacies, and degree of devotions to teaching profession. IBM SPSS Statistics 21program was used for t-test of differences among teaching profession ideologies, teacher efficacies, and degree of devotions to teaching profession. In addition, Pearson’s product-moment correlation coefficient was calculated to explore relations between teaching profession ideologies and teacher efficacies, teacher efficacies and degree of devotions to teaching profession, and teaching profession ideologies and degree of devotions to teaching profession. Results of the research are as followings. First, teaching profession ideologies of integrated kindergarten teachers do not show statistical significance regarding integrated education related training and completion of the courses. Second, teacher efficacies of integrated kindergarten teachers show statistical significance regarding integrated education related training and completion of its courses. Third, degree of devotions to teaching profession of integrated kindergarten teachers does not show statistical significance regarding integrated education related training and completion of its courses. Fourth, significant positive correlation is shown between two variables, teaching profession ideologies and teacher efficacies of integrated kindergarten teacher. Fifth, teacher efficacies and degree of devotions to teaching profession of integrated kindergarten teachers show positive correlation. Sixth, teaching profession ideologies and degree of devotions to teaching profession of integrated kindergarten teachers show significant positive correlation.

      • 커뮤니티 댄스를 통해 형성되는 라포 탐색 : 척도 개발을 중심으로

        김선희 경희대학교 체육대학원 2018 국내박사

        RANK : 247647

        The study found that the fundamental solution to the decline of human quality values in modern society, excessive individualism and serious social problems, lies in the restoration of human love and community consciousness. The purpose of this study was to identify the concept of composition of the rapport formed through community dance and to develop measures to measure the value of community dance to the rapport formation. Development of measuring tools was a priority in order to achieve the objectives of this study, and the research methods utilized for verification of validity and reliability after measurement tool development were of exploratory Factor analysis and a confirmatory factor analysis based on Delphi analysis techniques in the third stage. The results of the study are as follows: A total of 55 preformed content items were extracted through open questionnaires. And 39 preliminary surveys of 55 items that were closed questionnaires. Finally, the contents of 39 community dancing rappas were classified into 5 categories. "Positive Thinking", "Community Relations", "Stable Psychology", "Positive Relationship with Others", and "Overcoming Loneliness" were classified into 5 categories. The significance of building intimacy in community dance is to develop community dance activities that are well felt, well expressed, well enlightened, between community dance leaders, participants and among participants. It also has a positive effect on strengthening community dance solidarity. In community dancing, rapport formation has a positive effect on developing community dance activities. And it will be able to promote the community dance by effectively utilizing the formation of rapport of this community dance. That is, the characteristics of these identified participants can provide opportunities for community dance leaders to help improve the quality of relationships among community dance participants and improve the quality of life for each participant. It will contribute to a better community by directly linking all of the personal, social and public values gained through local dance from the results of this study. 본 연구는 현대사회에서 인간의 질적 가치의 하락, 과도한 개인주의, 심각한 사회문제 등에 있어서 근본적인 해결책이 인간애의 회복과 관계의 회복, 공동체 의식의 회복에 있으며, 그에 앞서 전제되어야 할 조건인 라포형성을 위한 적극적인 실천과 방법이 강구되어야 하며 이에 커뮤니티 댄스의 치유적 가치를 준거로 춤을 통해 형성되는 라포의 구성 개념을 규명하는데 목적을 두었다. 본 연구의 목적달성을 위하여 측정 도구의 개발이 우선적으로 필요하였으며 측정 도구 개발 후 타당성과 신뢰성 검증을 위해 활용한 연구 방법은 3단계 델파이 분석 기법을 기반으로 한 탐색적 요인분석 및 확인적 요인분석이었다. 연구 방법 절차로 도출된 결과는 개방형 설문을 통해 예비 추출한 55개의 라포형성 내용 항목이 추출되었다. 그리고 폐쇄형 설문지로 진행된 55개 문항의 예비조사는 39개로 추출되었다. 마지막으로 39개의 커뮤니티 댄스 라포형성 내용을 5개의 범주로 구분할 수 있다. 5개 범주로 구분된 “긍정적 사고”, “공동체 관계”, “안정적 심리”, “타인과의 긍정적 관계”, “외로움 극복”은 39개의 세부항목을 내포하였다. 커뮤니티 댄스에서 라포형성이 지니는 의미는 커뮤니티 댄스를 통해 잘 느끼고, 잘 표현하고, 잘 깨우치고, 커뮤니티 댄스 지도자와 참가자 간 그리고 참가자끼리의 사이에서 커뮤니티 댄스 활동을 발전시키고 커뮤니티 댄스 단체의 결속을 강화하는데 긍정적인 작용을 한다는 것이다. 그리고 이러한 커뮤니티 댄스의 라포형성을 효과적으로 활용하여 커뮤니티 댄스의 활성화를 도모할 수 있을 것이다. 커뮤니티 댄스의 지도자가 커뮤니티 댄스 일반 참여자들의 동기를 자극할 수 있는 프로그램을 개발하는 데 활용할 수 있다. 그리고 본 연구 결과를 바탕으로 커뮤니티 댄스 참가자들의 활동 참가 전, 참여자가 개인의 특성을 파악하는데 도움이 될 것이다. 즉 이렇게 파악된 참여자의 특성은 커뮤니티 댄스 참여자들 사이에 관계의 질을 개선하고, 참여자 개개인의 삶의 질을 향상시키는데 커뮤니티 댄스 지도자가 일조할 수 있는 기회를 제공할 수 있을 것이다. 또한, 공유와 공감의 시대를 열어 가야 하는 현대인들에게 집단지성으로서의 예술이라 할 수 있는 커뮤니티 댄스 참여자들이 경험하는 라포형성의 5가지 요인은 무용의 가치에 대한 실질적 접근과 통합적 적용을 가능하게 하는 준거로 활용될 수 있으며, 커뮤니티 댄스를 통해 체득되는 개인적 가치, 사회적 가치, 공적 가치를 모두 직접적으로 연결시킴으로써 더 나은 공동체 사회를 만드는데 기여할 것이다.

      • 교통사고 다발지역 원인 추론 분석과 인지적 요인을 통한 해석 연구 : 영등포 로터리 중심으로

        김선희 서울시립대학교 디자인전문대학원 2017 국내석사

        RANK : 247647

        The development of traffic creates new roads. In this process, new roads are added to existing roads, and roads with deformed structures are created. These deformed roads cause frequent traffic accidents which are also social problems. The government has been selecting the places where traffic accidents are frequent and making improvements every year, but traffic accidents are still not decreasing. Therefore, the researcher became aware of the guidelines of facilities could be wrong from the beginning, recognized the necessity of finding solutions by analyzing the possible causes, and proceeded with this research. Specifically, the researcher worked on the problems of traffic accidents in Yeongdeungpo Rotary using design analysis method. The Yeongdeungpo Rotary district is one of the areas where traffic accidents are the most frequent. The road environment in this area is very complicated and many drivers experience various forms of confusion. In recent years, various interpretations and solutions have been proposed to solve the problems caused by the complex structure of the area, but most of them are only measures to smooth traffic flow. There are still no fundamental measures to lower the rate of traffic accidents. If design analysis methods are applied to find out the causes of the frequent occurrence of traffic accidents in this area, it would be a great contribution to the establishment of a fundamental solution. As a result of this research, the causes of traffic accidents in the Yeongdeungpo Rotary Area are as follows: 1. Drivers can become confused because they believe they drive on the straight forward lane. 2. The ambiguousness of direction arrows gives confusion to drivers and disturbs their perception. 3. It is difficult for them to check their lane while driving. 4. In addition, if they stop while driving, it is difficult for them to interpret and connect all informations around them. 5. Therefore, at the point where drivers should decide their direction, the most needed information should be given priority. 6. Highly risky roads tend to have lots of informations, making it difficult for drivers to recognize all of them. 7. Errors are found in the contents of road signs: some of them are overly mixed or abbreviated. 8. There are lanes where road directions are given earlier than road signs. 9. It is difficult for drivers to check road signs of all directions. 10. The drivers’ view is not secured and they cannot prepare for any possible situation on the road. The purpose of this study is to investigate the causes of drivers’ cognitive errors in the Yeongdeungpo Rotary district through observations and analysis. The reason why there are lots of traffic accidents in the area is considered to be ‘self-generated by interactions between humans and the road environment.’ Of course, observational analysis can raise the problem of the objectivity, for subjective reasoning can intervene. Nevertheless, the researcher presents this study because traffic accidents are highly influenced by personal tendency of each driver, which is a set of factors that cannot be objectified. This means that objective reasoning can also be an useful method. Therefore, the causes of traffic accidents proposed here need to be verified by appropriate methods to propose new creative road designs. 교통의 발달은 새로운 도로를 생성시킨다. 이 과정에서 기존 도로에 새로운 도로가 더해지며 기형적인 구조의 도로가 만들어지기도 한다. 이러한 기형적인 구조의 도로는 교통사고가 다발로 일어나는 원인이 되어 사회적 문제로 대두되었다. 이에 정부는 교통사고가 잦은 곳을 선정하여 매년 개선 사업을 벌이고 있지만, 여전히 교통사고는 줄어들지 않고 있다. 따라서 연구자는 애초부터 시설물의 가이드가 잘못되어 있지 않느냐는 문제의식을 가지게 되었다. 그렇기에 연구자는 원인 유추를 통한 해결 방법이 강구되어야 할 필요성을 인식하여, 본 연구를 진행했다. 구체적으로, 본 연구에서는 영등포 로터리에서 발생하는 교통사고의 문제점을 디자인적 분석 방법으로 찾고자 했다. 상습적으로 발생하는 교통사고 다발지역 중에서 으뜸인 지역이 바로 영등포 로터리 지역이다. 이 지역의 도로 환경은 매우 복잡한 구조를 띠고 있어서 많은 운전자는 다양한 형태의 혼란을 경험하게 된다. 최근 들어, 이 지역의 복잡한 구조로 인해 발생하는 문제를 해결하기 위해 다양한 해석과 해법이 제시되고 있으나 그 대부분이 교통의 흐름을 원활하게 하기 위한 대책일 뿐이다. 근복적인 차원에서 교통 사고율을 낮추는 대책은 별로 제시되고 있지 못한 형편이다. 디자인적 분석 방법을 적용해 이 지역에서 교통사고가 왜 빈번하게 발생하는지에 대한 원인을 유추할 수 있다면 근본적인 해결책 마련에 있어 큰 기여가 가능할 것이다. 연구자의 연구 결과, 영등포 로터리 지역에서 교통사고가 많이 발생하는 원인은 다음과 같았다. 1. 운전자가 주행하는 차선이 직진 개념이 되기 때문에 오류를 범한다. 2. 이때, 모호한 화살표 방향은 운전자에게 혼란을 주어, 오히려 지각에 방해된다. 3. 운전자는 주행 중에 자신의 차선을 확인하기 어렵다. 4. 또, 운전자가 주행 중 정지하게 되면, 정보를 연결하여 판단하기가 어려운 환경이다. 5. 그러므로 방향 결정할 때, 운전자 위치에서 필요한 정보가 우선적으로 제공되어야 한다. 6. 위험도가 높은 도로는 정보량이 많아서 운전자가 모두 인지하기 어렵다. 7. 표지판의 내용이 합쳐져 있거나 축약 표기되어 있는 오류가 발견된다. 8. 노면에 안내표지판보다 앞선 지명을 표기한 오류가 발견된다. 9. 모든 방향에서 운전자가 표지판을 확인하기 어렵다. 10. 운전자의 시야가 확보되지 않아 주행로의 상황에 대비하지 못한다. 본 연구는 영등포 로터리 지역을 대상으로, 운전자가 인지적 오류를 범하는 원인을 관찰 분석을 통해 살피고자 하였다. 그 이유는 이곳에서 발생하는 교통사고의 문제가 ‘인간과 환경의 관계에서 자생되는 것’으로 판단하였기 때문이다. 물론 관찰 분석은 주관적 추론이 개입되기 때문에 객관성이 부족하다는 문제 제기가 가능하지만 그럼에도 연구 결과물로 제시하는 이유는 교통사고의 경우 개인적 성향에 큰 영향을 받는 것이어서 객관화할 수 없는 요인들의 집합이기 때문에 주관적 추론 또한 유용한 연구방법일 수 있다고 판단하기 때문이다. 따라서 주장된 원인 유추들은 적절한 방법으로의 검증이 필요하며 이를 통해 창의적인 디자인 제안이 가능하다고 판단한다.

      • 도시지역 일부 주부들의 자가투약 실태와 관련요인

        김선희 조선대학교 보건대학원 2006 국내석사

        RANK : 247647

        This study is to identify the current status of self-medication of housewives who have had experiences of self-medication for the last one year and analyse its factors to disclose their relations. To survey the recognition on instruction of pharmacists about medication, this study prepared self-reporting type questionnaire, targeting 600 housewives who are above twenties and have had self-medication for the past one year living in Gwangju city from September 15. 2005 to September 30. 2005. The results are presented as follows: Of the subjects who have had self-medication experience for the past one year, the ones who are self-medicated for the last two weeks were 24.6%. In respect to self-medication, most of the subjects bought medicine at pharmacy and relied on self-medication because the disease is not serious. The past experience of medication and advice from doctors or pharmacists were main influential factors on self-medication. A digestive, a pain pill, and cold tablets were the main subjects medicine of self-medication. This study found that the subjects learned information on self-medication through a manual. They read little or never the instructions because they already know how to use or the contents are complicating. In terms of economy, convenience, remedial effects and reliability, they were generally satisfied. For the last one year, 16.3% of the self-medicated subjects for the last one year and 13.5% of the subjects who had experiences of self-medication recently has side-effects. When a side-effect occurred, most of the stopped the medication. The instruction on medication was generally given, but it is not enough. Most of the instruction was about how to use the medicine and pharmacists' anxiety on purchasing of other medicine was thought to be obstacle to active instruction of medication. In respect to the instruction of medication, they complained about limited contents and asked more detailed information on side effects and effects of medication. Through the analysis, this study identified self-medication for recent two weeks and the relations between variables. As the subjects made more effort to maintain health, the rate of self-medication was significantly high (p=0.049). The grade of knowledge of the groups who were self-medicated on medicine was 5.0± 1.6, and that of the group who were not self-medicated was 4.6± 1.7. There was a significant difference (p=0.022). As frequency of the instruction on how to medicate by pharmacists was higher, self-medication rate was significantly high (p=0.022). However, attention to age, education, number of families, job, income, exercise, drinking, health, information on health, understanding on the instruction and help for instruction were not significantly related. In conclusion, this study found that 24.6 % of the housewives had experiences of self-medication for recent two weeks and 100% of the subjects had experiences of self-medication for the last one year. When self-medication is attempted, this study suggests that knowledge on medicine and drugs should be properly given, and pharmacists should provide higher-quality medication service and information as main information providers. However, this study suggests that further research on factors which are demonstrated to have relations with self-medication and induce proper self-medication behaviors are needed.

      • 연안 해역에서 분리한 해양 세균의 유류분해능 평가

        김선희 순천대학교 대학원 2017 국내박사

        RANK : 247647

        This study was performed for the purposes of bacterial diversity analysis in crude oil-contaminated coastal areas through petroleum oil enrichment cultures, evaluation of marine bacterial isolates for their biodegradation potential of oils, construction of bacterial consortia applicable for the bioremediation of oil-contaminated marine environment and investigation of effect of additional carbon and nitrogen sources on petroleum degradation, and analyses of genome and genes responsible for the degradation of oils. In bacterial diversity analysis, a total of eighty one colonies were randomly picked up from the marine agar(MA) plate containing diesel, gasoline, or kerosene and used for further studies such as bacterial identification and diversity analysis, biodegradation potential, etc. Bacterial identification study based on 16S rRNA revealed 21 species which belong to 12 genera (Advenella, Bacillus, Brevibacterium, Corynebacterium, Kytococcus, Microbacterium, Micrococcus, Paenibacillus, Pseudomonas, Rhodococcus, Staphylococcus, and Stenotrophomonas) and 3 phyla such as Actinobacteia, Firmicutes, and Proteobacteria (β-Proteobacteria and γ-Proteobacteria). Proteobacteria was predominant phylum and occurred in the frequency rate of 51.8% (γ-Proteobacteria, 35.8%; β-Proteobacteria 16.0%), Firmicutes, 28.4%, and Actinobacteia, 19.8%. A total of twenty one species were isolated from diesel, gasoline, or kerosene enrichment culture. Nineteen species isolated from diesel were Advenella kashmirensis, Bacillus idriensis, Bacillus infantis, Bacillus marisflavi, Bacillus oceanisediminis, Brevibacterium frigoritolerans, Corynebacterium xerosis, Kytococcus sedentarius, Microbacterium maritypicum, Microbacterium oxydans, Micrococcus yunnanensis, Paenibacillus glucanolyticus, Pseudomonas brassicacearum, Pseudomonas corrugata, Pseudomonas thivervalensi, Rhodococcus qingshengiidj, Staphylococcus xylosus, Staphylococcus saprophyticus, and Stenotrophomonas rhizophila. Five species from gasoline were Advenella kashmirensis, Microbacterium oxydans, Pseudomonas brassicacearum, Pseudomonas taeanensis, and Pseudomonas thivervalensi. Six species from kerosene were Advenella kashmirensis, Bacillus infantis, Bacillus oceanisediminis, Paenibacillus timonensis, Pseudomonas brassicacearum, and Pseudomonas thivervalensi. In particular, three species such as Advenella kashmirensis, Pseudomonas brassicacearum, and Pseudomonas thivervalensi were commonly isolated from all the media containing diesel, gasoline and kerosene used in this study. Two species, Bacillus infantis and Bacillus oceanisediminis were commonly isolated from both diesel and kerosene; only Microbacterium oxydans were commonly isolated from both diesel and gasoline. Paenibacillus timonensis and Pseudomonas taeanensis were the only bacterium which was isolated from kerosene and gasoline, respectively. In order to construct a marine bacterial consortia which are applicable to the bioremediation of marine environment contaminated with petroleum oils, three bacterial species Brevibacterium frigoritolerans SHD-34, Pseudomonas brassicacearum SHG-4, and Pseudomonas taeanensis MS-3 were selected owing to their outstanding abilities to degrade petroleum oils. Each bacterium was morphologically, physiologically, and genetically were characterized and evaluated for their oil degradation potential. B. frigoritolerans SHD-34 was Gram-stain-positive, while P. brassicacearum SHG-4 and P. taeanensis MS-3 were Gram-stain-negative. The optimal temperature, pH, and salinity of the three bacteria for their growth were 30℃, 7.0 and 3%, respectively. The common temperature and salinity ranges for their growth were 10℃ to 45℃ and 0% to 6%. Four sets of bacterial consortia (KⅡ-1, KⅡ-2, KⅡ-3, and KⅢ) were constructed and tested for their petroleum oil degradation abilities, resulting in the selection of two bacterial consortia, KⅡ-1 (a combination of SHD-34 and SHG-4) and KⅡ-2 (a combination of SHD-34 and MS-3). GC-MS analysis showed that both consortia, KⅡ-1 and KⅡ-2 were found capable of more than 90% degradation of petroleum oils such as gasoline and kerosene used in the level of 1% concentration. Additional carbon and nitrogen sources were used in the level of 1% and investigated for their effects on the degradation of 1% kerosene by P. brassicacearum SHG-4, P. taeanensis MS-3, B. frigoritolerans SHD-34, KⅡ-1 and KⅡ-2. Investigation of five carbon sources such as glucose, lactose, maltose, D-fructose, and citric acid showed that lactose was found to make most positive effective on kerosene degradation with highest increased degradation rate of 93.8 to 100%. Other carbon sources such as maltose, D-fructose, and citric acid were shown to make rather negative effects with reduced rate of 54.3% to 80.1% in kerosene degradation by individual bacteria. In analysis of kerosene degradation with four nitrogen sources such as NH4Cl, peptone, urea, and yeast extract, yeast extract was found to make most positive effect with 100% degradation rate of kerosene by all the tested individual and consortium bacteria. In GC-MS analysis on the effect of carbon sources on the degradation of n-alkane compounds in kerosene, alkane compounds (C7 - C19) were almost completely degraded in the range of 99.36 to 100% degradation rate with glucose or lactose by B. frigoritolerans SHD-34, which is very similar in degradation pattern by consortium KⅡ-2. P. taeanensis MS-3 exhibited very different degradation rate according to alkane compounds with glucose. Heptane(C7) - decane(C10) were degraded to the range of 90.1 to 100% with glucose by P. taeanensis MS-3, while undecane(C11) - nonadecane(C19), to the range of 51.5 to 71.0% with the same carbon source. By strain MS-3 were alkane compounds (C7 - C19) degraded to the range of 95.3 - 100% with lactose. Consortium KⅡ-1 exhibited very similar n-alkane compounds degradation rate (≃more 100%) in the absence or presence of lactose, maltose and D-fructose, while glucose and citric acid rather reduced the degradation rate pattern in effect of carbon sources. In effect of nitrogen sources, alkane compounds (C7 - C19) were almost completely degraded to 100% degradation rate with yeast extract by B. frigoritolerans SHD-34, P. brassicacearum SHG-4, P. taeanensis MS-3, KⅡ-1, and KⅡ-2, suggesting yeast extract was the most effective one of the four nitrogen sources to enhance the kerosene degradation rate. In GC-MS analysis on the effect of carbon sources on the degradation of aromatic compounds in kerosene, lactose was found most effective one. Benzene or benzene-type compounds, indene, and naphthalene were all degraded to 99.7% and 98.7% with lactose by B. frigoritolerans SHD-34 and P. taeanensis MS-3, respectively. There were some variations in degradation rate of aromatic compounds in kerosene from bacterial strain to bacterial strain, but lactose made an common enhanced effect on the degradation. Glucose showed an positive effect in strain SHD-34, SHG-4, and KⅡ-2. Interestingly, maltose made an adverse effect on the degradation of benzene, indene, and naphthalene of kerosene in individual strains (42.3% - 66.6% in degradation rate), except for bacterial consortia, KⅡ-1, and KⅡ-2 (100% in degradation rate). In effect of nitrogen sources, all the aromatic compounds identified in kerosene, benzene, indene, and naphthalene were completely degraded with enhanced degradation rate of 100% when yeast extract was added by all the individual strains and consortia. The other nitrogen sources (NH4Cl, peptone, and urea) did not have a significantly enhanced effect in degradation rate, and in most cases there were rather adverse effects. Analysis of alkB genes for the degradation of n-alkane compounds obtained through PCR amplification showed that all the three strains, B. frigoritolerans SHD-34, P. brassicacearum SHG-4, and P. taeanensis MS-3 include alkB genes. The amplified genes from B. frigoritolerans SHD-34, P. brassicacearum SHG-4, and P. taeanensis MS-3 were all identified alkane monooxygenase. Alkane monooxygenase gene from B. frigoritolerans SHD-34, P. brassicacearum SHG-4 and P. taeanensis MS-3 shared 99.4%, 85.6%, and 98.2% similarities, respectively in deduced amino acid sequences with those of Atrhrobacter sp. ITRH49, Diezia sp. ITRH56, Microbacterium sp. ITRH47, Pantoea sp. BTRH11, Peudomonas sp. ITRI53, Pseudomonas sp. MIXRI75, Ochrobacterum sp. ITRH1, and Rhodococcus sp. ITRH43. In particular, P. taeanensis MS-3 was selected due to its novelty and potential to degrade a broad spectrum of oil substrate, and its genome was completely analyzed, showing that total number of bases were 5,460,155, and the number of genes were 5,111. Identification of genes responsible for the degradation of petroleum oils suggested the possibility that strain MS-3 could degrade both alkane and aromatic hydrocarbons. Aromatic hydrocarbons like benzene or benzene-substituted compounds were suggested to be degraded into major intermediates catechol or protocatechuate through upper pathways. Degradation of catechol and protocatechuate through lower pathways was considered to be occupied by catechol 1,2-dioxygenase and protocatechuate 3,4-dioxygenase. It was considered that alkane compounds might be degraded into primary alcohol or secondary alcohol by alkane monooxygenase in P. taeanensis MS-3. In conclusion, B. frigoritolerans SHD-34, P. brassicacearum SHG-4, and P. taeanensis MS-3 were all found to be capable of degrading petroleum such as diesel, gasoline, and kerosene, as well as all of them had alkB genes responsible for the degradation of alkane compounds. Lactose and yeast extract were found most effective common carbon and nitrogen source, respectively to enhance the degradation rate of petroleum oils. Interestingly, intensive further degradation study using kerosene as a model substrate of petroleum oils resulted in the production of data showing comparative degradation rates of individual chemicals of alkane and aromatic hydrocarbons in kerosene. In biodegradation study using bacterial consortia, consortia KⅡ-1 (a combination of SHD-34 and SHG-4) and KⅡ-2 (a combination of SHD-34 and MS-3) exhibited enhanced kerosene degradation rate, compared with that of individual strains. Genome study on P. taeanensis MS-3, which was selected owing to its novelty suggested that it had redundant genes responsible for the degradation of alkane and aromatic hydrocarbons. Based on the identified genes, putative metabolic pathways for the degradation of alkane and aromatic compounds in petroleum oils were proposed.

      • 그림책 읽기 활동이 만2세 영아의 읽기 흥미도와 어휘력 발달에 미치는 영향

        김선희 우석대학교 2012 국내석사

        RANK : 247647

        국문초록 그림책 읽기 활동이 만2세 영아의 읽기흥미도와 어휘력 발달에 미치는 영향 아동복지 및 유아교육전공 김선희 지도교수 김경중 본 연구의 목적은 그림책 읽기 활동이 만2세 영아의 읽기흥미도와 어휘력 발달에 어떤 영향을 알아보는데 그 목적이 있다. 본 연구의 목적에 따라 연구에서 설정한 연구 문제는 다음과 같다. 1. 그림책 읽기 활동이 만2세 영아의 읽기 흥미에 미치는 영향은 어떠한가? 2. 그림책 읽기 활동이 만2세 영아의 어휘력에 미치는 영향은 어떠한 가? 본 연구의 연구대상은 J시에 위치한 D어린이집의 만2세반 10명과 M어린이집 만2세반 10명 총 20명을 대상으로 실시하였다. 영아의 읽기 흥미를 검사하기 위해 본 연구에서 읽기 흥미도는 PPRAI(Primanry Pupil Reading Attitude Inventory) (Askov & Fichbach, 1973)를 임원신(1994)이 번안, 수정한 읽기 흥미도 검사도구를 사용하였고, 어휘력 발달 측정은 김영태․장혜승․임선숙․백현정(1995)이 제작한 ‘그림 어휘력 검사’도구를 사용하였으며 자료분석은 대응 2-표본 비모수 검정(Wilcoxon 부호순위 검정), 독립 2-표본 비모수 검정(Mann -Whitney 검정), 독립 k-표본 비모수 검정(Kruskal-Wallis 검정)을 실시하였다. 본 연구결과는 다음과 같다. 첫째, 그림책 읽기 활동 후 영아의 읽기 흥미도에 있어서 통계적으로 유의한 차이가 있었다. 즉 그림책 읽기 활동 전 보다 그림책 읽기 활동 후 영아의 읽기 흥미도가 증가하였다. 그림책 읽기 활동 후 만2세 읽기 흥미도에 있어서 성별에 따라서는 사전검사와 사후검사 모두 통계적으로 유의한 차이가 나타나지 않았으며, 출생순위 또한 통계적으로 유의한 차이가 나타나지 않았다. 둘째, 그림책 읽기 활동 후 영아의 어휘력 발달에 통계적으로 유의한 차이가 있었다. 즉 그림책 읽기 활동 전 보다 그림책 읽기 활동 후 영아의 어휘력 발달이 증가하였다. 그림책 읽기 활동 후 만2세 어휘력 발달은 성별에 따라 사전검사와 사후검사 모두 영아의 어휘력에 통계적으로 유의한 차이가 나타나지 않았으며, 출생순위 또한 통계적으로 유의한 차이가 나타나지 않았다. ABSTRACT An Influence on Interest in 2 Year Children's Interesting in Reading and Vocabulary Developments of Picture Book Reading Activities Kim Seon Hee Major in Child Welfare and Childhood Education General Graduate School Woosuk University Supervised by Professor Kim Kyung Jung This study aims at looking into an influence on 2 year children's interest in reading and vocabulary developments of picture book reading activities. The subjects established in the study, according to the study, are as followings. 1. How is the influence on 2 year children's interest in reading of picture book reading activities? 2. How is the influence on 2 year children's vocabulary developments of picture book reading activities? The objects are 10 children, 2 year class, enlisted in D Kindergarten located at J city and 10 children, 2 year class, enlisted in M Kindergarten, total 20 children. In order to examine children's interest in reading, this study used an examination tool of the interest in reading produced with PPRAI (Primary Pupil Reading Attitude Inventory) (Askov & Fichbach, 1973) adapted and revised by Im Won Sik(1994), and an examination tool of picture vocabulary development produced by Kim Yeong Tae, Jang Hye Seung, Im Seon Suk, Baek Hyeon Jeong(1995). This study performed Paired Two Samples Test(Wilcoxon's Signed-ranks Test), Independent Two Samples Test (Mann- Whitney Test), and Independent K-Samples Test (Kruskal- Wallis Test) by non-parametric methods. The results are as followings. First, after reading picture books, children's interest in reading picture books showed a significant difference statistically. That is, children's interest in reading after reading picture books increased than before. 2 year children's interest in reading after reading picture books didn't show a significant difference statistically, according to sex and post-test and birth order didn't show a significant difference statistically and post-test. Second, after reading picture books, children's vocabulary development showed a significant difference statistically. That is, the development of children's vocabulary increased after reading picture books than before. After reading picture books, 2 year children's vocabulary development didn't show a significant difference statistically, according to sex and post-test and birth order didn't show a significant difference statistically and post-test.

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