가압가열 처리한 시판 돈육 소시지의 항원성에 미치는 소화효소의 영향

김서진,김꽃봉우리,송유진,이소영,윤소영,이소정,이청조,안동현,Kim, Seo-Jin,Kim, Koth-Bong-Woo-Ri,Song, Eu-Jin,Lee, So-Young,Yoon, So-Young,Lee, So-Jeong,Lee, Chung-Jo,Ahn, Dong-Hyun 한국축산식품학회 2009 한국축산식품학회지 Vol.29 No.2

Food allergy is a serious nutritional problem in both children and adults. Therefore, food allergenicity reduction methods are greatly needed. The allergenicity is altered by various manufacturing processes, and the digestibility of food proteins can be affected by food processing. This study was conducted to investigate the effect of in-vitro digestibility on the allergenicity of autoclaved market pork sausages using pepsin (30min) and trypsin (5, 30, 60, 90, and 120min). The binding ability of the porcine serum albumin (PSA) from sausages A and B significantly decreased by about 30 and 23%, respectively, after autoclave treatment (121; 5, 10, and 30 min). After the pepsin and trypsin treatments, the binding ability of products A and B at 30 min decreased. These competitive indirect enzyme-linked immunosorbent assay (ci-ELISA) results corresponded well with the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting results. The results demonstrated that the allergenicity of pork sausages considerably decreased after autoclave treatment, and were also maintained or decreased after enzyme treatment. Accordingly, autoclave treatment represents a promising processing technology for the reduction of the allergenicity of diverse food products.

가압가열 처리에 의한 시판 돈육 햄과 베이컨의 알레르겐성 저감화 효과

김서진,김꽃봉우리,송유진,이소영,윤소영,이소정,이청조,김규언,안동현,Kim, Seo-Jin,Kim, Koth-Bong-Woo-Ri,Song, Eu-Jin,Lee, So-Young,Yoon, So-Young,Lee, So-Jeong,Lee, Chung-Jo,Kim, Kyu-Earn,Ahn, Dong-Hyun 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.1

The pork hams and bacon comprising the most popular processed pork were treated with autoclave to investigate application of hypoallergenic pork. Among pork hams and bacon, two products with the highest binding ability were selected for experiments. The results of ci-ELISA on pork hams treated with autoclave showed that the binding ability of p-IgG and pigallergic patient's sera (P2) to PSA (porcine serum albumin) from pork ham samples by autoclave treatment at $121^{\circ}C$ for 30 min was slightly decreased. The binding ability to p-IgG of b and c bacon treated with autoclave was declined to below 16% and 11% as compared with control sample that showed 60% and 91% binding ability. The binding ability to P2 of b and c bacon treated with autoclave decreased to below 22% and 34% as compared with control sample that showed 95% and 126% binding ability. A result of immunoblotting on bacon showed that p-IgG as well as pig patient's sera did not recognize PSA well in autoclave treatment. The results obtained from this work indicated that autoclave treatment was effective for a reduction of allergenicity of pork hams and bacon. Therefore the autoclave treatment may be applied to development of hypoallergenic pork.

물리적 처리에 의한 돼지고기의 항원성 변화

김서진,김꽃봉우리,송유진,이소영,윤소영,이소정,이청조,박진규,이주운,변명우,안동현,Kim, Seo-Jin,Kim, Koth-Bong-Woo-Ri,Song, Eu-Jin,Lee, So-Young,Yoon, So-Young,Lee, So-Jeong,Lee, Chung-Jo,Park, Jin-Gyu,Lee, Ju-Woon,Byun, Myung-Woo,Ahn, D 한국축산식품학회 2009 한국축산식품학회지 Vol.29 No.6

The purpose of this study was to search for physical treatments to reduce allergenicity of pork. Physical treatments such as heating, autoclave, microwave, sonication, and irradiation have been used for food processing or reduction of allergenicity. The porcine serum albumin (PSA), known as a major allergen in pork, was extracted after physical treatments. The antigenicity of pork extracts by heating (80 and $100^{\circ}C$ for 20 min), autoclave ($121^{\circ}C$ for 5, 10, and 30 min), and microwave (for 5 and 10 min) was significantly decreased. Especially, the binding ability of p-IgG to pork extracts by autoclave for 30 min showed the greatest decrease (about 3%) in physical treatments. However, the antigenicity of pork was unaffected by sonication and irradiation treatment. These results indicated that the autoclave treatment was the most effective method to reduce the antigenicity of pork.

포스트 코로나를 대비하는 예술교육: 언택트 시대의 몸교육에 관한 소고

김서진 ( Seo-jin Kim ),문영 ( Young Moon ) 대한무용학회 2020 대한무용학회논문집 Vol.78 No.3

This study began as a pandemic shock urging us to change our way of life. Is body-based education in the age of untouched a crisis or an opportunity? It summarize an idea of arts education combined with technology that will be divided before and after COVID-19.

교육용 애플리케이션의 활용이 지적장애 초등학생의 푸드코트 이용하기에 미치는 효과

김서진 ( Kim Seo-jin ),장혜성 ( Chang Hae-song ) 대구대학교 한국특수교육문제연구소 2017 특수교육저널 : 이론과 실천 Vol.18 No.1

장애학생들이 일반적으로 동기가 낮고 흥미와 관심을 가지지 못하는 어려움이 있다는 점을 고려해 볼 때, 스마트러닝은 다양한 멀티미디어적인 기능의 활용을 가능하게 하므로 동기유발에 도움이 된다. 또한, 스마트폰의 교육용 애플리케이션은 다양한 종류의 수준별 교육용 콘텐츠를 보유하고 있어 지적장애 학생들의 능력에 맞는 적절한 맞춤형 개별학습을 제공할 수 있다는 장점을 지니고 있다. 그러므로 본 연구에서는 특수학급에 재학 중인 지적장애 초등학생 3명에게 교육용 애플리케이션을 활용한 지역사회중심교수를 실시하였을 때, 푸드코트 이용기술 수행에 미치는 영향과 다양한 장소에서 일반화되고 유지될 수 있는지를 알아보고자 하였다. 실험설계는 대상자간 중다간헐기초선 설계방법을 사용하였다. 교실중재는 교육용 애플리케이션 자료를 활용하여 푸드코트 이용기술을 교수하고, 지역사회중재는 중재패키지를 이용하여 실제 지역사회 푸드코트에서 교수하였다. 연구 결과, 교육용 애플리케이션을 활용한 지역사회 중심교수를 실시하였을 때 모든 대상학생들은 푸드코트 이용기술을 습득하였으며, 습득된 기술은 학습하지 않은 지역사회 푸드코트에서 일반화되었으며, 중재 종료 3주 후에도 유지되었다. 위와 같은 결과는 교육용 애플리케이션의 다양한 멀티미디어적인 기능이 장애학생들의 흥미와 관심을 이끌어 낼 뿐 아니라, 시공간의 제약 없이 학습이 언제 어디서나 즉시 이루어질 수 있기에 기술 습득에 효과적이라는 결론을 도출할 수 있었다. A purpose of this study is to know that elementary school students with intellectual disabilities can maintain their learned knowledge and skills through educational application of smart learning after passing over 4 weeks. To be specific, the study wants to know whether three students who had been educated by community-based instruction can use these skills again at the same food court and do that by themselves. Meantime, the multiple probe design across participants had been used as an experimental design in the study. At the arbitration stage, students had been educated with educational application, various strategies for the arbitration at the same time. In the generalization stage, students had been experienced new food court three times during specific time. After this, maintenance test was conducted three times as well. To conclusion of this study, the community-based instruction with educational applications of smart learning is an effective and useful method for students with disabilities to obtain food court or other places using independently.

폐경 후 골감소증 여성에 대한 12주간의 영양교육과 운동 중재 전,후식품 및 영양소 섭취량 변화와 골밀도 지표 변화와의 관계

김서진(Seo Jin Kim),강서정(Suh Jung Kang),박윤정(Yoon Jung Park),황지윤(Ji Yun Hwang) 대한지역사회영양학회 2013 대한지역사회영양학회지 Vol.18 No.3

Few studies investigated the effects of nutrition education and exercises in women with osteopenia. This study examined the relationship between changes in dietary intakes and changes in indicators related to bone health in postmenopausal women with osteopenia (?2.5 ≤ T-score ≤ 1) after a 12-week intervention. Thirty-one postmenopausal women aged > 50 years residing in Seoul were recruited and participated in nutritional education regarding bone health and general nutrition practices and aerobic exercises (three times a week; 60 min per session). Twenty-five subjects completed the study and were eligible for the analysis. Bone mineral density (BMD) at femoral neck was measured by dual energy x-ray absorptiometry. Serum calcium, osteocalcin, and intact parathyroid hormone (PTH) were also measured. Dietary intake was estimated by using a one-day 24 recall by a clinical dietitian. After 12 weeks, meat consumption increased (P = 0.028) but vegetable intake decreased (P = 0.005). Intakes of animal protein (P = 0.024), vitamin B1 (P = 0.012) and vitamin B2 (P = 0.047) increased, and sodium intake decreased (P = 0.033). Intact PTH (P = 0.002) decreased and osteocalcin (P = 0.000) increased, however, BMD decreased (P = 0.000). Changes in mushroom consumption were positively correlated with femoral neck BMD (r = 0.673, P = 0.003). Changes in animal iron intake were negatively correlated with intact PTH (r = ?0.488, P = 0.013) but were positively correlated with osteocalcin (r = 0.541, P = 0.005). These results suggested that the association between animal iron intake and biochemical markers of bone turnover may play an important role in bone metabolism. Further studies are needed to shed light on complicated mechanisms of diet, hormonal levels of bone metabolism, and bone density. (Korean J Community Nutr 18(3) : 213~222, 2013)

외상환자 수액 투여에서 알루미늄 호일을 이용한 단열 효과

김서진 ( Seo Jin Kim ),선경훈 ( Kyung Hoon Sun ),박용진 ( Yong Jin Park ),김선표 ( Sun Pyo Kim ) 대한외상학회 2014 大韓外傷學會誌 Vol.27 No.2

Purpose: The temperature of a warm fluid infused into a patient is lowered because the line that allows the fluid to be infused into the patient is exposed to room air. This study evaluated the effects of aluminum foil used as an insulator surrounding the fluid infusion lines when using warm crystalloid fluids to treat traumatic shock patients. Methods: The study measured the differences in fluid temperature between infusion lines with and without the aluminum-foil insulation. We used 1L of normal saline at 40。C as the infusion fluid, and the fluid infusion line was 200 cm long. The differences in temperature were measured for various fluid flow rates from 12,000 mL/min to 100 mL/min. We performed three experiments at each flow rate. Results: The results showed the differences in temperature between the groups with and without the aluminum insulation were significant for flow rates above 100 mL/min. Conclusion: Hypothermia in trauma patients results in many adverse complications such as peripheral vascular constriction, tissue hypoxia, metabolic acidosis, heart dysfunction and so on. Thus, the use of warm fluids and blood components is essential to reduce the probability of hypothermia. This study showed the aluminum foil wrapped around the infusion line had an insulator effect. As a result, such a wrapping can be used to avoid the adverse effects of hypothermia.

삼지구엽초로부터 분리한 Icariin의 생리활성

김서진(Seo Jin Kim),박명수(Myoung Su Park),딩티안(Tian Ding),왕준(Jun Wang),오덕환(Deog Hwan Oh) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.10

본 연구는 삼지구엽초의 기능성 식품학적 이용성 증진을 위한 기초적인 연구로써 우리나라 산야에서 존재하며 예로부터 약용으로 이용되고 있는 삼지구엽초의 주요성분인 icariin을 분리하여 생리활성을 검토하였다. 에탄올로 추출한 삼지구엽초를 극성이 다른 용매인 에틸아세테이트, 부탄올, 헥산, 물, 클로로포름 층으로 분리하여, icariin의 함량을 측정한 결과 각각 27.9, 2.5, 1.7, 1.4, 1.3 μg/g이었고, icariin 수율이 가장 많은 에틸아세테이트 분획물로부터 정제한 icariin 수율은 295.5 μg/g이었으며 대부분 에틸아세테이트에서 용출되는 것을 확인하였다. 용매별 분획물의 항산화 효과는 에틸아세테이트 분획층에서 49.0 μg/mL로 가장 높았으며, 부탄올(59.2 μg/mL), 헥산(119.8 μg/mL), 물(122.0 μg/mL), 클로로포름(138.5 μg/mL) 층의 순으로 나타났으며, 정제된 icariin(15.3 μg/mL)은 대조구인 ascorbic acid(19.5 μg/mL), α-tocopherol(18.2 μg/mL)보다 다소 높은 활성을 나타내었다. Salmonella Typhimurium을 이용한 돌연변이 및 항돌연변이성에 미치는 영향을 조사한 결과, 삼지구엽초에서 분리한 icariin은 TA98과 TA100세포 모두에서 돌연변이성을 나타내지 않았다. 인간면역세포인 B세포와 T세포에서의 면역활성시험을 측정한 결과, icariin은 대조구보다 B세포에서 1.27배, T세포에서 1.28배의 비교적 높은 면역증강활성을 나타내었다. 이러한 결과는 삼지구엽초를 이용한 기능성식품 및 식품재료로서의 이용성 증진을 위한 기초자료로 제공될 수 있을 것으로 판단된다. Epimedium koreanum Nakai is a wild medicinal plant commonly consumed in South Korea due to its health beneficial effects. In the present study, the antioxidative, antimutagenic and immunological activities of E. koreanum Nakai extracts were investigated for their use in food. The yields of icariin compounds from the ethanol extract as well as the ethyl acetate, butanol, hexane, water, and chloroform fractions of E. koreanum were 27.9, 2.5, 1.7, 1.4, and 1.3 μg/g, respectively. The icariin components (295.5 μg/g) were collected from the ethyl acetate fraction by thin layer chromatography (TLC) and analyzed via high performance liquid chromatography (HPLC). The antioxidant activities of each fraction were as follows: ethyl acetate (49.0 μg/mL), butanol (59.2 μg/mL), hexane (119.8 μg/mL), water (122.0 μg/mL), and chloroform (138.5 μg/mL), based on RC₅₀ μg/mL. Icariin, isolated and identified as the main component, showed strong antioxidant activity with a RC₅₀ value of 15.3 μg/mL, which was higher than those of ascorbic acid (19.5 μg/mL) and α-tocopherol (18.2 μg/mL). In an Ames test, none of the fractions produced mutagenic effects on Salmonella Typhimurium TA98 and TA100. In an immunomodulating activity test, the effects of E. koreanum Nakai on B cells (Rhamos) and T cells (Jurkat) were investigated. These results show that the growth and viability of B and T cells were increased by isolated icariin components for 1.27 and 1.28 fold, respectively. These results also provide preliminary data for the development of E. koreanum Nakai as an edible food material.

탄소 및 은 잉크 기반의 전위차 나트륨 이온 센서 제조 및 이의 전기화학적 특성

김서진 ( Seo Jin Kim ),손선규 ( Seon Gyu Son ),윤조희 ( Jo Hee Yoon ),최봉길 ( Bong Gill Choi ) 한국공업화학회 2021 공업화학 Vol.32 No.4

본 연구에서는 탄소 및 은 잉크를 사용하여 스크린 인쇄 공정을 통한 전위차 나트륨 이온(Na⁺) 센서를 제작하였다. 센서의 두 전극 구성은 Na⁺ 용액에서 네른스트 거동에 따라 전극의 전위차를 발생하였다. 제조된 Na⁺ 센서는 이상적인 네른스트 민감도, 빠른 응답 시간 및 낮은 검출 한계를 보여주었다. 네른스트 반응은 센서의 반복성 및 장기 내구성 테스트 시 안정적이었다. 탄소 전극에 코팅된 Na⁺ 선택막은 간섭 이온에 대해 나트륨 이온을 선택적으로 통과시켜 우수한 선택성을 증명하였다. 휴대용 Na⁺ 센서는 인쇄 회로 시스템을 사용하여 제작되었으며 다양한 실제 샘플에서 Na⁺ 농도를 성공적으로 측정하는 것을 증명하였다. A potentiometric sodium-ion (Na⁺) sensor was prepared using a screen-printing process with carbon and silver inks. The two-electrode configuration of the sensor resulted in potential differences in Na⁺ solutions according to Nernstian equation. The obtained Na⁺-sensor exhibited an ideal Nernstian sensitivity, fast response time, and low limit of detection. The Nernstian response was stable when the sensor was tested for repeatability and long-term durability. The Na⁺-selective membrane coated onto the carbon electrode selectively passed sodium ions against interfering ions, indicating an excellent selectivity. The portable Na⁺-sensor was finally fabricated using a printed circuit system, demonstrating the successful measurements of Na⁺ concentrations in various real samples.

지하역사 내 미세먼지 실시간 모니터링을 위한 광산란법 보정

김서진(Seo-Jin Kim),강호성(Ho-Seong Kang),손윤석(Youn-Suk Son),윤상렬(Sang-Lyeor Yoon),김조천(Jo-Chun Kim),김규식(Gyu-Sik Kim),김인원(In-Won Kim) 한국대기환경학회 2010 한국대기환경학회지 Vol.26 No.5