Ultra Real-Time PCR을 활용한 Avian Influenza Virus Subtype의 조기진단법

김상태,김영균,김장수,Kim, Sang-Tae,Kim, Young-Kyoon,Kim, Jang-Su 한국미생물학회 2011 미생물학회지 Vol.47 No.1

조류 인플루엔자 바이러스(AIV) 아형을 ultra-time PCR법(UPCR)을 이용하여 초스피드로 진단할 수 있는 방법을 고안하였다. 표적 대상의 프라이머는 AIV H5N1 아형의 hemagglutinin(HA) 유전자 중 가장 상보성이 높은 133 bp의 부위를 선택하였고, 실험의 안전을 위하여 인공합성의 방법으로 제작하였다. 압타머와 결합한 molecular beacon 기반 Mini-Opticon Q-PCR 기기를 사용한 UPCR법으로, 총 UPCR 반응액의 양을 10 ${\mu}l$으로, UPCR과 용융온도 분석시간을 15분 이내로 매우 짧게 단축시켰다. 민감도 측정에서 최소의 주형인 5분자의 HA 유전자만으로 정확히 AIV의 특이적 133 bp를 합성하였다. UPCR로 디자인된 이 PCR은 AIV 아형의 진단에 적용될 수 있을 뿐 아니라, UPCR이 기반되는 진단을 이용하여 다른 병원체에도 널리 적용 될 수 있을 것으로 기대된다. This ultra real-time PCR (UPCR) based diagnosis system for avian influenza A virus (AIV) subtype was designed. The target primer in this study was derived from H5N1 subtype-specific 133 bp partial gene of hemagglutinin (HA), and was synthesized by using PCR-based gene synthesis on the ground of safety. UPCR was operated by Mini-Opticon Q-PCR Quantitative Thermal Cycler using aptamer-based molecular beacon, total 10 ${\mu}l$ of reaction mixture with extraordinarily short time in each steps in PCR. The detection including UPCR and analysis of melting temperature was totally operated within 15 min. The AIV-specific 133 bp PCR product was correctly amplified until 5 molecules of HA gene as minimum of templates. This kind of PCR was drafted as UPCR in this study and it could be used to detect not only AIV subtype, but also other pathogens using UPCR-based diagnosis.

흰쥐 정소세포에서의 Cadmium 독성 작용과 Selenium 전처리의 영향

김상태,부문종,최임순 ( Sang Tae Kim,Moon Jong Boo,Rim Soon Choe ) 한국환경생물학회 1990 환경생물 : 환경생물학회지 Vol.8 No.1

To investigate the cadmium toxicity to testis and the effect of selenium pretreatment, male rats were subcutaneously injected with Cd and/or Se prior to Cd. In doing so, the respiration rates of mitochondria, the lysosomal activities of acid phosphatase and the cellular levels of GSH were measured. Also, the testicular ultrastructures were observed. Cd only-treated group showed that the respiration rates of mitochondria, the lysosomal activities of acid phosphatase and the level of GSH were reduced on the whole. In contrast to above group, Se-pretreated group appeared to be recovered: the respiration rate was restored more than that of control group and the enzyme activity was restored somewhat, however less than that of the control group. The level of GSH was recovered to the level of control group in Se-pretreated group. According to ultrastructural studies, Cd-treated group showed the swelling and destruction of mitochondria and the production of lipid droplets. In contrast, Se-pretreated group appeared not to be different from control group. So, all of these results would indicate that Se has a protective function against Cd toxicity in the rat testis.

감은사의 사리장엄에 의한 2탑구성원리에 관한 연구

김상태,Kim, Sang-Tae 한국건축역사학회 2007 건축역사연구 Vol.16 No.2

The purpose of this article is making investigation of the principal composition of the twin-pagodas in Buddhist temple through ornaments of a case preserving relics of Buddha in the east and west pagodas of Gameunsa temple. This research has an important meaning in clarifying that the concept of placement two Buddhas together with lotus sutra, which was only applied to Bulkuksa temple, has been extended to Gameunsa temple. And this observation gave us a clear explanation for the principal composition at the twin-pagodas in Buddhist temple plans. The author obtained the following conclusion from this article : The cases preserving relics of the Buddha for the west and east pagodas are globally in similar form as the type of a royal palace. But the different points between eastern and western can be seen on the existence of Buddhist statues on the base floor, set-up of the gate at railings, and 4 lion statues at a coner of the base floor. In the western pagoda, there are Buddha statues above the base-floor of the cases preserving relics. These Buddha statues are composed of a boy monks and angels with playing music for the praise of the Sakyamuni Buddha lecturing the Lotus sutra at YoungChui Mountain. On the other hand, in the eastern pagoda, Buddha statues on the cases preserving relics are composed of a the Four Devas and monks as a defending God of the nation of Buddha. In the eastern pagoda, one can see something that does not exist in the western pagoda, which is a gate located in railing of a case preserving the relics. This gate described the scene of the Sakyamuni Buddha entering into the pagoda of 7 treasures. In a corner of the case preserving relics in the eastern pagoda, there is another thing which does not exist in the western pagoda. This is a sculpture of a lion which symbols the seat of Lion supporting the Buddha in the Dabotap(the pagoda of 7 treasures). When we observe the form and structure, the descriptions at the railings, the gate, the shrine, the statue of a lion, and the accessories in jewelries, at the cases preserving the relics in the eastern pagoda, one can realize that they have the same combination structures between the ones at Dabotaps in Bulguksa temple and those in China as well as in Japan, all of which represent the Gyun-Bo-Tap-Pum of Lotus Sutra. Among the distribution rules in the twin-pagodas in Buddhist temple, the application of the ideal of lotus was known to be effected only to Bulguksa temple. But from the result of the present article, it is more clearly proved by applying the Gameunsa temple that the distribution theory in the twin-pagodas in Buddhist temple was in fact based on the ideal of lotus.

정신병과 건강생활-정신건강이란 무엇인가

김상태,Kim, Sang-Tae 한국건강관리협회 1976 건강소식 Vol.4 No.9

직장인의 건강관리-직장인의 스트레스와 정신장애

김상태,Kim, Sang-Tae 한국건강관리협회 1987 건강소식 Vol.11 No.1

직장인의 스트레스와 나아가 이로 인한 정신장애의 극복책은 우선적으로 스트레스의 바른 해소책 개발과 인격적 취약성의 자각 및 인격 성숙을 위한 부단한 노력 이다.

현대인의 히스테리를 진단한다

김상태,Kim, Sang-Tae 한국건강관리협회 1989 건강소식 Vol.13 No.5

피로수명예측을 위한 잔류강도 저하모델의 파라미터 결정법 제안(II)

김상태,장성수,Kim, Sang-Tae,Jang, Seong-Su 대한기계학회 2001 大韓機械學會論文集A Vol.25 No.9

A new method of parameter determination in the fatigue residual strength degradation model is proposed. The new method and minimization technique is compared experimentally to account for the effect of tension-compression fatigue loading of spheroidal graphite cast iron and graphite/epoxy laminate. It is shown that the correlation between the experimental results and the theoretical prediction on the fatigue life and residual strength distribution using the proposed method is very reasonable. Therefore, the proposed method is more adjustable in the determination of the parameter than minimization technique for the prediction of the fatigue characteristics.

지식정보화 사회를 대비한 특수재능보유자 전형

김상태,Kim, Sang-Tae 한국대학교육협의회 2000 大學敎育 Vol.105 No.-

꿈과 수면

김상태,Kim, Sang-Tae 한국건강관리협회 1993 건강소식 Vol.17 No.12

주입선 형태가 타이타늄 합금의 주조성에 미치는 영향

김상태,방몽숙,양홍서,박상원,박하옥,임현필,Kim, Sang-Tae,Vang, Mong-Sook,Yang, Hong-So,Park, Sang-Won,Park, Ha-Ok,Lim, Hyun-Pil 대한치과보철학회 2007 대한치과보철학회지 Vol.45 No.4

Statement of problem: Despite of the recent development of the titanium casting system methods, the casting defects such as imperfect casting and internal porosity were frequently observed. Purpose: The purposes of this study were to compare and measure the castability, microhardness, and surface reaction between Grade 2 pure titanium and Ti-6Al-4V by casting these alloys from the different sprue design conditions. Material and methods: Depending on the sprue designs and titanium alloys, 42 ready-made wax patterns were used. By analyzing the remodeling of the cast, internal porosity, microhardness, and titanium surface layer of SEM, there were several results we observed. Results: 1. The measured castability of titanium were categorized in the ascending order: individual sprue group, runner bar group, and single group. This data are based on the statistically signigicant differences. 2. The castability of titanium has not showed the statistically significant differences among the alloys. However, CP-Ti groups were superior to Ti-6Al-4V groups by showing the noticeable castability. 3. The surface layers of the castings of all groups have showed $5{\mu}m$ titanium oxide layers irrespective of sprue designs and titanium alloys. Conclusion: From the above study results, by fabricating the restorations from the centrifugal casting machine direct sprue designs revealed better castability. As we increased the number of sprues in the wax pattern, it revealed better castability. The castability of pure titanium rather than that of Ti-6Al-4V was remarkable. To fabricate the complex forms of the restorations, further researches on the efficient sprue designs and titanium alloys must be made.