Populus tomentiglandulosa 와 P . glandulosa 의 분류학적 연구

김삼식,김정석,야전소삼 (野田昭三) ( Sam Sik Kim,Chung Suk Kim,Shozo Noda ) 한국산림과학회 1981 한국산림과학회지 Vol.51 No.1

Populus glandulosa and Populus tomentiglandulosa, which were known to be natural hybrids, were examined for morphological, physiological and karyological traits to illucidate its hybridity and taxonomical importance. The results abtained were as follows; 1. Survival rate in rooting of cuttings and grafting was different between the hybrids and their rooting abilities showed incomplete dominance. 2. Their leaf openings showed incomplete dominance. The leaf longevities of P. alba × glandulosa and P. tomentiglandulosa were stronger than the other hybrids. 3. There were differences in resistance to toxicity of KC10, between the hybrids. 4. Many external leaf characters of the hybrids also showed incomplete dominance. P. tomentiglandulosa was similar in those characters to P. alba × glandulosa while P. glandulosa was similar to hybrids crossed, reciprocally crossed or back-crossed between P. davidiana and P. alba. 5. Their numbers of male flower showed incomplete dominance or hybrid vigor. The numbers of P. tomentiglandulosa were similar to thosa of P. alba × glandulosa while those of P. glandulosa to those of P. alba × davidiana or P. davidiana × alba. 6. Morphology and band color of male catkin bract showed incomplete dominance. Those of P. glandulosa were similar to those of P. alba × davidiana while those of P. tomentiglandulosa to those of. P. alba × glandulosa. 7. There were differences in vascular bundle number and arrangement of petiole between the hybrids. 8. Differences in the anatomical traits of stem did not exist between the hybrids but those in wood fiber size existed. 9. The chromosomes of artificial hybrids, P. glandulosa and P. tomentiglandulosa showed irregular behavior in metaphase I and II. 10. All hybrids including P. glandulosa and P. tomentiglandulosa showed small number of P.M.C. with 19 II but many univalent chromosomes were exhibited in metaphase I. 11. All hybrids including P. glandulosa and P. tomentiglandulosa showed a little abnormal nuclear plates as laggard chromosome and chromosome bridge in anaphase I and II. 12. The frequency of pollen tetrad and fertile pollen was low in most of the hybrids including P. glandulosa and P. tomentiglandulosa.

상피성 종양에서 Microsatellite Instability 분석

김삼식,박일수 대한부인종양학회 2006 Journal of Gynecologic Oncology Vol.17 No.4

Objective : Some genetic alterations is involved in ovarian carcinogenesis. Since some benign and borderline tumors may represent early steps in ovarian carcinogenesis, analysis of precursor lesions could provide evidence that an accumulation of genetic events is required in order for normal ovarian epithelium to generate benign, borderline, malignant tumors. Few pre-invasive ovarian tumors have so far been studied. Methods :60 cases of ovarian epithelial tumor, including benign, borderline, and malignant tumors, were analyzed for microsatellite instability (MSI) by gel analysis of paired germ line and tumor DNA. PCR amplification was performed using the panel of 5 microsatellite markers recommened by the NCI (BAT25, BAT26, D2S123, D5S346, D17S250) and 6 additional markers (D1S160, D1S162, D7S522, D11S860, D17S855, D17S932). Results : In this study, D2S123 and D5S346 were the most frequently altered markers in malignant ovarian tumors and D11S860 locus showed MSI in 4 adenomas, 4 boderline tomors, and 10 malignant tumors. Other markers displayed instability with only one or two tumors showing MSI. On the basis of NCI criteria, most benign tumors demonstrated microsatellite stable (MSS). In the borderline tumors, 10 tumors revealed MSS, 8 tumors had low-frequency MSI (MSI-L), and two tumors had high frequency MSI (MSI-H). In the malignant tumors, 6 tumors revealed MSS, 2 tumors had MSI-L, and 12 tumors had high MSI-H. Conclusion : According to the result, MSL-H is more frequently seen in the malignant tumors. D2S123 and D5S346 were the most frequently altered markers in the malignant tumors, and D11S860 locus may be involved in early step of carcinogenesis. 목적 : Microsatellite는 개인의 각각의 세포에 일생동안 고정된 염기서열을 가지며 유전체에 분포하는 nucleotide의 짧은 일렬반복 (tandem repeat)이다. 이러한 MSI는 mismatch repair의 가장 중요한 지표가 되어 대장암 외 유방암, 자궁내막암, 위암 등 다른 장기의 암에서도 연구가 활발히 진행되고 있다. 연구 방법 : 본 연구에서는 경북대학교병원에서 수술로 절제된 난소 조직 중 양성, 경계성 및 악성종양 각 20 예 등 총 60 예의 상피성 난소종양을 대상으로 하였다. 종양조직과 정상조직을 종양세포와 정상세포를 보다 세밀하게 분리할 수 있는 미세절제술을 이용하여 MSI 변이의 빈도가 높다고 알려진 11종의 microsatellite marker를 이용, MSI 분석을 하여 양성, 경계성 및 악성 종양에서 유전자의 변이를 조사하고자 한다. 결과 : 11종의 microsatellite marker 중 D2S123과 D5S346은 양성종양에서는 MSI 변이가 발견되지 않았지만 경계성 종양에서는 각각 30% 및 25%, 악성종양에서는 각각 60% 및 55%에서 변이가 있는 것으로 각각 분석되었다. D11S860의 경우, 악성종양에서 20예 중 10예가 변이를 보였으며 양성종양과 경계성 종양의 경우 각각 20예 중 4예에서 변이가 발견되었다. 나머지 marker에서는 1예나 2예에서 변이를 보였을 뿐이며 양성종양과 경계성 종양에서도 1예 정도는 변이가 발견되었다. Microsatellite marker 분석 결과를 NCI의 정의에 따라 MSI-H, MSI-L, microsatellite stable (MSS)로 분류하면, 양성종양에서 MSI-H인 경우는 없었으며 대부분이 MSS로 분석되었다. 경계성 종양에서는 MSI-H 2예, MSI-L 8예, MSS는 10예로 나타나 양성종양보다는 변이가 일어난 예가 증가했음을 알 수 있다. 반면, 악성종양에서는 MSS 6예, MSI-L 2예, MSI-H가 12예로 나타나 양성종양(0%)이나 경계성 종양(10%)에 비해 MSI-H의 비율 (60%)이 크게 증가했음을 확인하였다. 결론 : 연구에서 사용한 microsatellite marker중 D2S123과 D5S346의 변이가 악성종양에서 높은 비율로 발견되었으며 D11S860은 양성종양이나 경계성 종양에서 비교적 높은 비율로 변이가 검출되는데 이러한 소견은 이 염색체에 포함된 종양억제 유전자 등이 난소암의 악성진행 또는 초기발생에 관여되었을 것이라 추정한다.

임목종자의 활력검정에 관하여

김삼식 한국양묘협회 1976 韓國養苗協會誌 Vol.4 No.-

남북 : 집중분석 ; 개성공단이 사는 길

김삼식 평화문제연구소 2003 통일한국 Vol.229 No.-

자웅이주성 옻나무속 3수종의 자연집단에서 성비와 성간 생장량 및 공간적 분포

김삼식,이정환,정재민 ( Sam Shik Kim,Jeong Hwan Lee,Jae Min Chung ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.2

Sex ratios, and patterns of tree size and growth variation, resource allocation, spatial and age class distribution between the sexes were investigated in natural populations of the sexual trees, R. trichocarpa and R. sylvestris, and the mainly asexual, clonal tree, R. javanica of the dioecious Rhus (Anacardiaceae) distributed in Korea. Sex ratios for three species exhibited a significant degree of female bias, but among the populations, sex ratios were seen to vary quite widely. The measurement of tree size and annual increment of male trees in R. trichocarpa and R. sylvestris were significantly higher than those of female trees, but not significantly different in R. javanica. In all of the species, flowering branch number per individual and inflorescence number per branch of males outnumbered those of females. Branch number per individual, rachis(leaf) number per branch and rachis(leaf) number per inflorescence were more in females than in males. These results were considered as reproductive efforts to increase the pollen supply in males and the fruit production in females. Spatial distribution analysis in two different populations of R. trichocarpa indicated that males and females were randomly distributed in space, but seedlings were clumped around parental trees. Analysis of age class distributions between the sexual reproduction trees, R. triclaocarpa and R. sylvestris, and the asexual, clonal tree, R. javanica showed a different distribution in frequencies of males and females in each age class. These results showed that sexual and asexual reproduction tree species had almost different preference of habitats, and different sex ratio and annual growth.

북한의 투자관리 제도의 변화와 전망

김삼식 中央大學校 民族發展硏究院 2003 民族發展硏究 Vol.- No.8

측백나무 과내의 (科內) 몇 수종과 (樹種) 화백 변종의 (變種) 동위효소에 관한 연구

김삼식 한국육종학회 1976 한국육종학회지 Vol.8 No.2

Populus 속의 엽병의 (葉柄) 특징에 의한 분류학적 연구

김삼식,황증 ( Sam Sik Kim,Jeung Hwang ) 한국산림과학회 1979 한국산림과학회지 Vol.43 No.1

The morphological and anatomical features of petioles in genus Populus were as follows, 1. All of 8 species had one sort of these trichoma; wooly, tomentose, gravellate, cravellate. 2. The colors of petioles were different with the species; Populus alba was green white, P. glandulosa was white green, and P. maximowiezii P. koreana were red on the surface but the back of petioles were light green. Most of petioles of P. euramericana and P. deltoides were light green but the rest on the same trees were two different colors as P. nigra var. italica and P. simonii were light green or yellow green. 3. P. koreana had the largest diameter of petiole among the 8 species investigated, and P. simonii was the smallest. 4. The lengths of petioles were highly significant among species except 3 combinations. 5. The number of vascular was different with the species and with section levels of petiole; At the base level, the vascular number of 8 species were highly significant among species and the average number of vascular of 8 species were 6.61. At the middle level, the average number of vascular were 5.26 in 8 species, and P. nigra var. italica were highly significant against the rest 7 species and two combinations showed at 5 percent significance. 6. At the terminal level, since the 95.5 per cent of the samples contained 3∼4 vasculars and only 4.5 per cent of the samples in each section contained 5 vasculars, there, was no significance among the species. 6. Mostly the largest vascular of petioles was appeared in under part of the any sections in 8 species investigated.

난소종양 환자의 혈중 및 면역 조직학적 CEA의 연구

김삼식,박일수,김광수,이윤순,김대한,김세진,황순구 대한부인종양 콜포스코피학회 1998 Journal of Gynecologic Oncology Vol.9 No.1

This study was performed to assess the significance of plasma level and histochemical character of carcinoembryonic antigen(CEA) in early diagnosis and prognosis of ovarian tumor. Plasma level of CEA was measured using EIA method and immunohistochemical tissue staining of CEA was done using biotin-strepto avidin complex immunoperoxidase technique. The percentage of patients with positive CEA level(above 2.5 ng/ml) was 23.1%(6/26) in malignant ovarian tumor and 15.6%(12/77) in benign ovarian tumor. Positive tissue staining of CEA was 42.3%(11/26) in malignant ovarian tumor and 19.5%(15/77) in benign ovarian tumor. In histologic typing, positive tissue staining of CEA was 18.1%(2/11) in serous cystadenocarcinoma, 85.7%(6/7) in mucinous cystadenocarcinoma, 37.5%(3/8) in other malignant ovarian tumors, 7.1%(1/15) in serous cystadenoma, 7.1%(1/14) in mucinous cystadenoma and 27.1%(13/48) in other benign ovarian tumors. Among 5 cases of malignant ovarian tumors with positive CEA level, 3 cases(60%) showed positive tissue staining of CEA, whereas among 21 cases of malignant ovarian tumors with negative CEA level, 8 cases (38.1%) showed positive tissue staining of CEA. However, among 11 cases of benign ovarian tumors with positive CEA level, 4 cases(36.4%) showed positive tissue staining of CEA, whereas among 66 cases of benign ovarian tumors with negative CEA level, 11 cases(16.7%) showed positive tissue staining of CEA. In the 3 year follow-up study of 12 cases with malignant ovarian tumor, among 3 cases with positive tissue staining of CEA, 2 cases(66.7%) survived. In 9 cases with negative tissue staining of CEA, 6 cases(66.7%) survived. In conclusion, these results suggest that the measurement of tumor CEA may be of value in the differential diagnosis of malignant and benign ovarian tumor, especially in diagnosing mucinous cystadenocarcinoma. However, due to the small amount of cases available for study, it was difficult to determine the correlation between the prognosis and tissue CEA staining of ovarian tumors.$quot;

남북경협의 현황과 활성화를 위한 과제

김삼식 한국시멘트협회 2003 시멘트 Vol.161 No.-