해외 기술: LOHAS 시대에 대응하는 Sous-vide 기술의 특징

김병목,김영명,Kim, Byoung-Mok,Kim, Young-Myoung 한국식품연구원 2010 食品技術 Vol.23 No.1

홍게(Chionoecetes japonicus) 다릿살 분말을 첨가한 어묵의제조 및 품질특성

김병목 ( Byoung Mok Kim ),정지희 ( Jee Hee Jung ),정민정 ( Min Jeong Jung ),김동수 ( Dong Soo Kim ),전준영 ( Joon Young Jun ),정인학 ( In Hak Jeong ) 한국수산과학회 2016 한국수산과학회지 Vol.49 No.1

This study investigated the physicochemical and sensory characteristics of fish paste containing red snow crab Chionoecetes japonicus leg-meat powder (RMP) in 0, 3, 6, 9, and 12% concentrations. The moisture contents of fish paste with RMP ranged from 56.7 to 60.2% and the pH ranged from 7.0 to 7.7. The L values decreased as the RMP concentration increased, and a and b increased. The folding test for all of the fish paste samples scored AA, which indicates good flexibility. The hardness, springiness, gumminess, and cohesiveness increased with the RMP concentration. In the sensory evaluation, the fish paste prepared with 6% RMP was the most preferred. These results suggest that RMP can be added to fish paste to achieve high quality.

홍게(Chionoecetes japonicus) 다릿살 분말 첨가가 국수의 품질 특성에 미치는 영향

김병목 ( Byoung Mok Kim ),정민정 ( Min Jeong Jung ),김종찬 ( Jong Chan Kim ),전준영 ( Joon Young Jun ),김동수 ( Dong Soo Kim ),정인학 ( In Hak Jeong ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.3

In this study, we investigated the quality characteristics of noodles supplemented with lyophilized leg-meat powder from the red snow crab, Chionoecetes japonicus (CP). The noodles were prepared by adding 0, 2, 4, 6, 8, 10% of CP on a wheat-flour basis. The ash, crude protein and crude lipid contents of noodles increased with increasing CP content. The cooked weight, volume and water absorption values of noodles decreased, whereas the turbidity increased, with increasing CP content. The L values of the noodles decreased with increasing CP content, whereas the a and b color values increased. The hardness, gumminess and chewiness values increased with increasing CP content increased, while springiness and cohesiveness showed no significant differences among the groups. In the sensory evaluation, cooked noodles with 4-6% CP had greater overall acceptability than the noodles with other CP levels.

HepG2 cell에서 식용수산자원 추출물의 지질축적억제효과

김병목(Byoung-Mok Kim),정지희(Ji-Hee Jung),김동수(Dong-Soo Kim),김영명(Young-Myoung Kim),정인학(In-Hak Jeong) 한국식품과학회 2012 한국식품과학회지 Vol.44 No.3

수산물 추출물로부터 간세포내 지질축적억제효과 추출물의 탐색을 통해 지방간 형성 억제 활성물질을 밝힐 목적으로, 각 48종의 수산물 추출물의 사람 간암세포유래인 HepG2 세포주에서의 중성지질축적억제 효과를 조사하였다. 그 결과, 18개의 추출물에서 유의적인 지질축적억제효과를 보였고, 그 중 Pagrus major, Octopus minor, Enteroctopus dofleini, Undaria pinnatifida Sporophyll, Styela clava, Enteromorpha intenstinalis 등 6개의 추출물이 유효한 효과가 있음을 관찰하였다. 이상의 결과를 바탕으로 지질대사에 관여하는 단백질들의 발현 정도를 조사하고, 활성물질의 분리 및 정제 등에 관한 연구와 지방간 형성 억제 효과에 대한 기전 연구가 진행되어야 할 것으로 사료된다. This study was performed to promote the effective utilization of edible marine resources and to develop functional food material from edible marine resource extracts for inhibiting lipid accumulation in liver. Edible marine resource extracts (ME) were prepared by hot water (MWE) and 80% ethanolic (MEE), and both the MWE and MEE extracts were assessed as to their cell cytotoxicity, and Oil Red O staining. Results demonstrated that ME showed no cytotoxic effects. However, treatment with MEE in the concentration of 1000, 500, and 250 ㎍/mL significantly inhibited the lipid accumulation in HepG2 cells compared to MWE. Especially, among the 80% ethanolic extracts, Pagrus major, Larimichthys polyactis, Clupea pallasii, Octopus minor, Enteroctopus dofleini, Styela clava, dried sea mustard, and Enteromorpha intestinalis showed greater lipid accumulation inhibitory activity than the others.

홍게(Chionoecetes japonicus) 다릿살 분말을 첨가한 마들렌의 제조 및 품질특성

김병목 ( Byoung Mok Kim ),정민정 ( Min Jeong Jung ),전준영 ( Joon Young Jun ),김동수 ( Dong Soo Kim ),정인학 ( In Hak Jeong ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.3

In this study, we investigated the quality characteristics of madeleine combined with lyophilized red snow crab Chionoecetes japonicus leg-meat powder (CM). The madeleine was prepared by the addition of wheat flour containing 0, 1, 5, 10, 15 and 20% concentrations of CM. Moisture content, pH, and baking loss rate did not differ significantly among groups. Light coloration significantly decreased in a dose-dependent manner, while red and yellow coloration significantly increased. Hardness, chewiness and gumminess significantly increased in a dose-dependent manner, while cohesiveness, springiness and adhesiveness did not differ from CON (madeleine containing 0% CM). In terms of sensory characteristics, appearance and color did not differ compared to CON, while taste, flavor and overall preference increased in a dose-dependent manner, with CM10 having the highest score among the groups. The present study assesses the potential of this improved formula as a convenience food.

강원도 시판 막장제품의 이화학적 품질특성 및 생리활성 조사

김병목(Byoung-Mok Kim),정지희(Jee-Hee Jung),임지훈(Ji-Hoon Lim),정민정(Min-Jeong Jung),정재형(Jae-Whung Jeong),최용석(Yong-Suck Choi),심재만(Jea-Man Sim),정인학(In-Hak Jeong),김영명(Young-Myoung Kim) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.6

본 연구에서는 강원도에서 생산되는 전통 막장제품(MJ) 12종을 수집하여 이화학적 품질특성 및 생리활성 효과에 대해 조사하였다. pH는 4.43~5.69로 나타났고 MJ5가 가장 높았으며, 염도는 11.1~16.9%로 분포되어 있었고 MJ3이 가장 높았다. 명도는 26.2~36.9, 적색도는 3.9~11.5, 황색도는 6.5~16.6으로 분포되어 있어 제품들 간에 뚜렷한 차이를 보였다. 아미노태질소 함량은 제품에 따라 342.41~605.92 mg%로 나타났고 MJ2가 가장 높았으며, 유리아미노산 총량은 1,175.3~4,657.7 mg%로 다양하게 분포되어 있었다. 특히 MJ11의 유리아미노산 총량은 4,657.7 mg%로 제품들중 가장 높았고, 주요 아미노산은 glutamic acid, aspartic acid, alanine, lysine, leucine, isoleucine이었으며, 제품에 따라 GABA 함량이 높게(MJ12, MJ8, MJ4) 나타나기도 하였다. 지방산 총량은 1,598.6~2,874.4 mg%로 MJ10이 가장 높았고, 주요 지방산으로는 linoleic acid, palmitic acid, linolenic acid, oleic acid, stearic acid였으며, 오메가 6지방산이며 필수지방산인 linoleic acid가 지방산 총량 대비 약 37.7~46.7%를 차지하였다. 총 폴리페놀 함량은 제품에 따라 401.48~746.67 μg GAE/mL로 분포되어 있었고, MJ11이 가장 높은 함량을 보였다. DPPH 및 ABTS 라디칼소거능은 총 폴리페놀 함량이 높은 MJ11, MJ8 및 MJ4가 가장 높았고, SOD 유사 활성은 MJ12 및 MJ2가 가장 높았다. 대장암세포주에 대한 세포 성장 억제 효과는 MJ1과 MJ12가 가장 높았고, 위암세포주에 대해서는 MJ3과 MJ5가, 유방암세포주에 대해서는 MJ5가 가장 높았다. 간세포에 대한 중성지질 축적 억제 효과는 MJ7이 가장 우수하였고, 항고혈압 효과는 MJ11이 가장 높았다. 이상의 결과에서 아미노태질소 함량과 유리아미노산 총량이 높은 막장제품뿐만 아니라, 항산화, 항암, 항고혈압 등의 효과를 보이는 막장제품들의 주요 원부재료는 메주, 보리, 고추씨 등이었으며 (자료 미기입), 이들 원부재료의 배합비에 따라 함량과 효능이 조금씩 다르게 나타났다. 향후 막장제품의 표준 제조방법 및 기준을 마련하기 위해서는 상기 결과들을 기초로 하여 막장 원부료의 정밀분석, 제조원료에 따른 제품들 간의 상관분석 등 보다 심도 있는 연구가 필요할 것으로 생각된다. In this study, we investigated the physiochemical properties and biological activities of Gangwon-do endemic Makjang (MJ) products (12 types). The pH levels of all samples were in the range of 4.43 to 5.69, and MJ5 showed the highest pH (5.69). The salinities of all samples ranged from 11.1% to 16.9%. Hunter color values for L (lightness), a (redness), and b (yellowness) ranged from 26.2 to 36.9, 3.9 to 11.5, and 6.5 to 16.6, respectively. The amino nitrogen content of MJ2 was highest, whereas the total content of free amino acids of MJ11 (4,657.7 mg%) was highest. Total fatty acid contents of all samples ranged from 1,598.6 mg% to 2,874.4 mg%, with MJ10 showing the highest fatty acid content. The content of total polyphenolic compounds ranged from 401.48 to 746.67 μg tannic acid equivalent/mL, with MJ11 showing the highest content. The 1,1-diphenyl-2-picrylhydrazyl and 2,2"-azino- bis(3-ethylbenzothiozoline-6-sulfonic acid) radical scavenging effects of MJ11, MJ8, and MJ4 were 51.30% and 82.5%, 41.29% and 67.0%, and 49.88% and 87.7%, respectively. MJ12 showed the strongest growth inhibitory effect on lung cancer A549 cells, whereas MJ5 showed the strongest growth inhibitory effect on AGS gastric cancer cell and MCF-7 breast cancer cell. MJ7 showed greater lipid accumulation inhibitory activity in HepG2 cells than the others. ACE inhibitory activity of MJ11 was the highest among the samples.

해조류 메탄올 추출물의 항산화효과

김병목(Byoung Mok Kim),전준영(Joon Young Jun),박영범(Yeung Beom Park),정인학(In Hak Jeong) 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.8

본 실험에는 강원도의 대표적인 갈조류로 재래종다시마, 괭생이모자반, 그리고 수심이 비교적 깊은 곳에 식생하는 곰피와 구멍쇠미역, 녹조에 속하는 고리매, 우리나라 국민이 가장 많이 소비하고 있는 해조이면서 홍조류에 속하는 김과 동해안 특산 홍조류이며 지역민들이 즐겨 먹고 있는 지누아리의 해조 7종이 사용되었다. 이 7종의 해조로부터 75% 메탄올로 상온에서 추출하여 얻어진 추출물을 용매를 완전히 제거한 후 다시 메탄올에 용해하는 메탄올 가용성 분획(SF)과 메탄올에 녹지 않지만 물에 용해하는 극성이 강한 성질의 불용성 분획(ISF)으로 분리하여 각 분획의 함량을 측정하였으며 DPPH를 이용한 전자공여능과 hydroxy radical 소거능을 측정하여 항산화효과를 분석하였다. 메탄올 가용성 분획은 구멍쇠미역, 고리매, 괭생이모지반이 26.60~23.40%로 높았으며 재래종다시마와 김이 9.26%와 8.66%로 낮았다. 메탄올 불용성이며 수용성 분획은 재래종다시마와 김에서 29.70%와 21.01%로 가장 높았으며 그 외의 해조에서는 지누아리 8.76%, 곰피 6.46%, 고리매 4.87%, 구멍쇠미역 2.05%, 괭생이모자반 1.95%로 아주 낮았다. DPPH를 이용한 전자공여능 평가와 hydroxy radical 소거능 평가에서 메탄올 가용성 분획이 높은 해조, 즉 구멍쇠미역과 괭생이모자반에서 우수한 항산화효과를 보였으며 이와 같은 평가결과는 총페 놀 함량이 높은 것과 잘 일치하였다. 갈조류인 곰피는 전자공여능은 우수한 것으로 평가되었으나 hydroxy radical 소거능은 나타나지 않았다. 메탄올 추출물에서 재용해하지 않는 메탄올 불용성 분획은 실험에 사용된 7종의 해조 모두에서 총페놀이 거의 없는 것으로 나타났으며 전자공여능과 hydroxy radical 소거능 평가에서 효과를 전혀 보이지 않았다. 이상의 결과로부터 해조의 항산화효과는 메탄올에 용해 하는 성분이 강한 항산화효과를 지니는 것으로 나타났으며 이 효과는 페놀 화합물과 관련이 깊은 것으로 사료된다. In this study, seven species of seaweeds (Sargassum horneri, Grateloupia filicina, Kjellmaniella crassifolia, Porphyra tenera, Ecklonia stolonifera, Scytosiphon lomentaria, Agarum cribrosum) from Gangwon coast were collected for antioxidative assays. The methanol soluble and insoluble (water soluble) fraction which were prepared from 75% methanol extraction were assayed by DPPH method and deoxyribose assay. The antioxi-dative activity of methanol soluble fraction of Agarum, Scytosiphon and Sargassum reached to 26.60%, 24.28% and 23.40%, respectively. The antioxidative activities of methanol insoluble fractions of the Kjellmaniella and Porphyra were assayed to 29.70% and 21.01% which were higher than methanol soluble fractions with the values of 9.26% and 8.66%, respectively. The results from DPPH assay and deoxyribose assay showed the methanol soluble fractions of Agarum and Sargassum have strong antioxidative activity. However the methanol insoluble (water soluble) fractions of seaweeds did not show any meaningful antioxidant activity excepted Kjellmaniella and Porphyra.

도루묵(Arctoscopus japonicus) 어묵의 제조 및 품질특성

김병목 ( Byoung Mok Kim ),김동수 ( Dong Soo Kim ),정인학 ( In Hack Jeong ),김영명 ( Young Myoung Kim ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.5

This study examined the effect of pretreated sandfish Arctoscopus japonicus meat as a surimi complex for preparing sandfish flavored fish paste. To prepare the sandfish- flavored paste, fine chopped sandfish meat including backbone was added in a ratio of 0 to 50 wt.% to thawed Alaska pollock Theragra chalcogramma surimi to make a mixed surimi gel. To prepare the sandfish-flavored paste, the mixed surimi was ground with salt using a silent cutter, mixed with starch and stabilizers 0.2% transglutaminase and gluconolactone 0.3%, stuffed in a rectangular container, left for 3 h at 25℃, cooked in hot water for 30 min at 90℃, and finally chilled for 20 min at 4℃. The effects of the pretreat-ment of sandfish meat were investigated by analyzing the quality of the paste produced. The proximate composition of FP (fish paste containing 40% steam-cooked sandfish meat and 0.3% gluconolactone) was moisture 76.1%, crude protein 12.0%, crude fat 3.8%, carbohydrate 6.1%, and ash 2.0%. The major minerals in FP were Na (23.77 mg/L), Mg (1.46 mg/L), Zn (1.04 mg/L), and Fe (0.41 mg/L), and the major free amino acids were taurine, anserine, alanine, and glutamic acid. The monounsaturated fatty acid content of FP was 566.22 mg%, and the polyunsaturated fatty acid content was 498.43 mg%. The n-3 fatty acid content was 398.01 mg%, and C20:5n-3 (218.85 mg %) was a major component.

백돈피와 흑돈피의 이화학적 품질특성 평가

전기홍,황윤선,김영붕,최윤상,김병목,김동욱,장애라,Jeon, Ki-Hong,Hwang, Yoon-Seon,Kim, Young-Boong,Choi, Yun-Sang,Kim, Byoung-Mok,Kim, Dong-Wook,Jang, Aera 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4

In order to determine the material characteristics of pork rinds according to the breeds, the chemical composition, color, pH, collagen contents, shear force, fatty acid and amino acid contents of pork rinds were investigated. White pork rinds (WPR) and black pork rinds (BPR) were evaluated before and after heating to compare the differences between breeds and the effects of heating treatment. In the chemical composition test, the respective moisture contents for WPR and BPR before heating were 49.90% and 53.75% but increased to 60.75% and 61.09% after heating. The test for crude protein and crude fat contents showed lower values after heating but WPR was higher than BPR. In the color test, the L value decreased rapidly with heating, dropping from 68.75 to 45.11 in WPR and from 67.22 to 49.64 in BPR. WPR had a higher L value and a lower a value than BPR before heating but had a higher L value and a lower a value than WPR after heating. pH was significantly higher in WPR than BPR regardless of heating (p<0.05). The collagen content for WPR and BPR was 10.38 g/100 g and 11.54 g/100 g but increased to 12.00 g/100 g in WPR and decreased to 11.40 g/100 g in BPR after heating. The shear force of 26.14 kgf in WPR was significantly higher than 12.89 kgf in BPR before heating (p<0.05), but the values decreased significantly after heating in both WPR and BPR. Linoleic acid in WPR was 17.29%, which was higher than 15.13% in BPR. The USFA for BPR was also higher than WPR. In amino acid composition, the EAA contents in WPR was 7,190 mg%, which was higher than 5,520 mg% in BPR.

산란노계와 육계의 이화학적 품질특성 평가

전기홍,황윤선,김영붕,최윤상,김병목,김동욱,장애라,Jeon, Ki-Hong,Hwang, Yoon-Seon,Kim, Young-Boong,Choi, Yun-Sang,Kim, Byoung-Mok,Kim, Dong-Wook,Jang, Aera 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.4

This study was performed to investigate the phyico-chemical characteristics of breast meat from spent hens (SP) in comparison with the same part from the broilers (BR). The moisture and crude protein contents for SP were 72.56% and 24.26%, which were lower than 75.87% and 24.64% for BR. Crude fat and crude ash contents in SP were 0.45% and 1.00%, which were higher than the BR contents of 0.41% and 0.51%. The respective L, a and b value for SP were 48.61, 2.40 and 2.42, which were lower than the BR values of 49.41, 1.45 and 3.06 respectively. The pH for SP was 5.89, which was higher than pH 5.79 for BR. The WHC for SP was 50.29%, which was lower than that of BR at 62.31%. SP scored 28.04% in the heating loss test, which was significantly higher than 19.09% for BR. The shear forces for SP and BR were 4.86 kg and 1.36 kg respectively, which meant that the texture of SP was much tougher than that of BR. Hardness for SP was 8.89 kg while that of BR was 3.92 kg indicating that the SP texture is firmer than that of BR. Oleic acid was most abundant fatty acid in both samples and was at 44.15% in SP and 27.68% in BR.