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- 저자 : 김미광(Mi Kwang Kim) (검색결과 23 건)
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한국인의 치주질환 환자의 치은연하치면세균막에서 Prevotella intermedia와 Prevotella nigrescens의 검출율 비교
김미광 ( Mi Kwang Kim ),성진효 ( Jin Hyo Seong ),김동기 ( Dong Kie Kim ),손영남 ( Young Nam Son ),이장재 ( Jang Jae Lee ),김흥중 ( Heung Joong Kim ),박주철 ( Joo Cheol Park ),장현선 ( Hyun Seon Jang ),김병옥 ( Byung Ock Kim ),신 대한예방치과·구강보건학회 2004 대한구강보건학회지 Vol.28 No.2
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분자생물학적 기법을 이용한 악골 골수염 병소의 세균 동정
김미성(Mi-Sung Kim),김수관(Su-Gwan Kim),정해만(Hae-Man Chung),김생곤(Sang-Gon Kim),국중기(Joong-Ki Kook),김미광(Mi-Kwang Kim),김화숙(Hwa-Sook Kim),유소영(So Young Yoo) 대한구강악안면외과학회 2003 대한구강악안면외과학회지 Vol.29 No.1
The purpose of this study was to isolate and identify the bacteria in osteomyelitis lesion of 3 patients. Two lesions were due to the post-infection after extraction. The other was resulted from mal-fixation of both sides of mandibular angles. Pus samples were collected by needle aspiration from the lesion and examined by culture method. Bacterial culture was performed in three culture systems (anaerobic, CO2, and aerobic incubator). Identification of the bacteria was performed by 16S rRNA gene cloning and nucleotide sequencing method. Our results showed that Streptococci species was predominantly isolated in both lesions of extraction socket. Only one species (Proteus vulagris) was detected in lesion of mandibular angle. This study was not sufficient to identify the causative bacteria in those osteomyelitis. However, our data may be offered the clue to solve the problem.
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상악동염 병소 부위에서 세균의 분리 동정 및 항생제 감수성에 대한 연구
최영옥(Young-Og Choi),김수관(Su-Gwan Kim),김학균(Hak-Kyun Kim),김영종(Yong-Jong Kim),최동국(Dong-Kook Choi),김미광(Mi-Kwang Kim),박순낭(Soon-Nang Park),김민정(Min-Jung Kim),국중기(Joong-Ki Kook) 대한구강악안면외과학회 2006 대한구강악안면외과학회지 Vol.32 No.5
The purpose of this study was to isolate and identify the bacteria in chronic maxillary sinusitis (CMS) lesions from 3 patients and to determine the antimicrobial susceptibility of them against 10 antibiotics. One of them was odontogenic origin and the others were non-odontogenic origin. Pus samples were collected by needle aspiration from the lesions and examined by culture method. Bacterial culture was performed in three culture systems (anaerobic, CO2, and aerobic incubator). Identification of the bacteria was performed by 16S rRNA gene (16S rDNA) nucleotide sequencing method. To test the sensitivity of the bacteria isolated from the maxillary sinusitis lesions against seven antibiotics, penicillin G, amoxicillin, tetracycline, ciprofloxacin, cefuroxime, erythromycin, clindamycin, and vancomycin, minimum inhibitory concentration (MIC) was performed using broth dilution assay. Our data showed that enterobacteria such as Enterobacter aerogenes (30%), Klebsiella pneumoniae (25%), and Serratia marcescens (15%) were predominately isolated from the lesion of non-odontogenic CMS of senile patient (70 year old). Streptococcus spp. (40.3%), Actinomyces spp. (27.4%), P. nigrescens, M. micros, and P. anaerobius strains were isolated in the lesion of odontogenic CMS. In the lesion of non-odontogenic CMS, Streptococcus spp. (68.4%), Rothia spp. (13.2%), and Actinomyces sp. (10.5%) were isolated. The susceptibility pattern of 10 antibiotics was determined according to the host of the bacteria strains ratter than the kinds of bacterial species. Even though the number of CMS was limited as three, these results indicate that antibiotic susceptibility test must be accompanied with treatment of CMS. The combined treatment of two or more antibiotics is better than single antibiotic treatment in the presence of multidrug-resistant bacteria in the CMS lesions.
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용이한 Subcloning을 위한 pCAT(R)3-Basic vector의 변형 2
유소영 ( So Youog Yoo ) , 김미광 ( Mi Kwang Kim ) , 김화숙 ( Hwa Sook Kim ) , 최민호 ( Min Ho Choi ) , 임상수 ( Sang Soo Lim ) , 박헌동 ( Heon Dong Park ) , 송수근 ( Soo Keun Song ) , 신환선 ( Hwan Seon Shin ) , 박슬희 ( Seul H 조선대학교 구강생물학연구소 2003 Oral Biology Research (Oral Biol Res) Vol.27 No.2
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DNA probe Pn25를 이용한 Prevotella intermedia 및 Prevotella nigrescens의 동정
김화숙 ( Hwa Sook Kim ) , 김미광 ( Mi Kwang Kim ) , 유소영 ( So Young Yoo ) , 최민호 ( Min Ho Choi ) , 임상수 ( Sang Soo Lim ) , 박헌동 ( Heon Dong Park ) , 송수근 ( Soo Keun Song ) , 신환선 ( Hwan Seon Shin ) , 박슬희 ( Seul H 조선대학교 구강생물학연구소 2003 Oral Biology Research (Oral Biol Res) Vol.27 No.2
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감염근관에서 분리 배양한 세균의 수종 항생제에 대한 감수성 조사
임상수,김미광,민정범,김민정,박순낭,황호길,국중기,Lim, Sang-Soo,Kim, Mi-Kwang,Min, Jeong-Beom,Kim, Min-Jung,Park, Soon-Nang,Hwang, Ho-Keel,Kook, Joong-Ki 한국미생물학회 2006 미생물학회지 Vol.42 No.3
The aim of this study was to identify the bacteria isolated from endodontic lesions by cell culture and to determine the antimicrobial susceptibility of them against 8 antibiotics. The necrotic pulpal tissues were collected from 27 infected root canals, which were diagnosed as endodontic infection. Samples were collected aseptically from the infected pulpal tissue of the infected root canals using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing $500{\mu}l\;of\;1{\times}PBS$. The sample solution was briefly mixed and plated onto a BHI-agar plate containing 5% sheep blood. The agar plates were incubated in a $37^{\circ}C$ anaerobic chamber for 2 to 5 days. The bacteria grown on the agar plates were identified by comparison of 16S rRNA gene (rDNA) sequencing method at the species level. To test the sensitivity of the bacteria isolated from the infected root canals against 8 antibiotics, minimum inhibitory concentrations (MIC) were determined using broth dilution assay. The data showed that 101 bacterial strains were isolated and were identified. Streptococcus spp. (29.7%) and Actinomyces spp. (21.8%) were predominantly isolated. The 9 strains were excluded in antimicrobial susceptibility test because they were lost during the experiment or were not grown in broth culture. The percentage of bacteria susceptible for each antibiotic in this study was clindamycin, 87.0% (80 of 92); tetracycline, 75.0% (69 of 92); cefuroxime axetil, 75.0% (69 of 92); amoxicillin + clavulanic acid (5:1), 71.7% (66 of 92); penicillin G, 66.3% (61 of 92); erythromycin, 66.3% (61 of 92); amoxicillin, 44.6% (41 of 92); and ciprofloxacin, 31.5% (29 of 92). The susceptibility pattern of 8 antibiotics was dependent on the host of the bacteria strains rather than the kinds of bacterial species. These results indicate that antibiotic susceptibility test should be performed when antibiotics are needed for the treatment of infected root canals.
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