잔류물질분석의 국제공인시험기관 인정 관련 고찰

김미경,조병훈,김동규,윤선종,임채미,박수정,김희진,김연희,김수연,윤소미,권진욱,손성완,정갑수,이주호,강문일,Kim, MeeKyung,cho, Byung-Hoon,Kim, Dong-Gyu,Yun, Seon Jong,Lim, Chae-Mi,Park, Su-Jeong,Kim, Heuijin,Kim, Yeon Hee,Kim, Soo-Yeon,Yun, So Mi,K 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.4

Residual materials such as veterinary drugs, environmental contaminants, and pesticides are affecting food safety. High resolution techniques and quality controls are needed to analyze these materials from part per million to part per trillion quantities in food. In order to achieve quality results, standardized methods and techniques are required. Our laboratories were prepared to obtain a certificate of accreditation for ISO/IEC 17025 in the analytical criteria of animal drugs, dioxins, pesticides, and heavy metals. ISO together with IEC has built a strategic partnership with the World Trade Organization with the common goal of promoting a free and fair global trading system. ISO collaborates with the United Nations Organization and its specialized agencies and commissions, particularly those involved in the harmonization of regulations and public policies including the World Health Organization and CODEX Alimentarius for food safety measurement, management and traceability. Our goal was to have high quality analysts, proper analytical methods, good laboratory facilities, and safety systems within guidelines of ISO/IEC 17025. All staff members took requirement exams. We applied proficiency tests in the analysis of veterinary drugs (nitrofuran metabolites, sulfonamide and tetracyclines), dioxins, organophosphorus pesticides, and heavy metals (Cd, Pb, As) to the Food Analysis Performance Assessment Scheme (FAPAS) at Central Science Laboratory, Department for Environment Food and Rural Affairs (DEFRA), England. The results were very satisfactory. All documents were prepared, including system management, laboratory management, standard operational procedures for testing, reporting, and more. The criteria encompassed the requirements of ISO/IEC 17025:1999. Finally, the Korea Laboratory Accreditation Scheme (KOLAS) accredited our testing laboratories in accordance with the provisions of Article 23 of the National Standards Act. The accreditation will give us the benefit of becoming a regional reference laboratory in Asia.

수입 식육의 안전성 확보를 위한 polychlorinated dibenzo-p-dioxins와 dibenzofurans 분석

김미경,김동규,김수연,최시원,권진욱,윤선종,송성옥,정갑수,Kim, MeeKyung,Kim, Dong-Gyu,Kim, Sooyeon,Choi, Si-Weon,Kwon, Jin-Wook,Yun, Seon Jong,Song, Sung Ok,Chung, Gab Soo 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.1

Polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) are ubiquitous and can contaminate the food chain. A study monitoring PCDD/Fs in imported meat was conducted at the National Veterinary Research and Quarantine Service (NVRQS, Republic of Korea) in order to maintain food safety from the bioaccumulative PCDD/Fs. Seventeen PCDD/Fs with toxic equivalency factors (TEFs) established by World Health Organization (WHO, 1998) were analyzed in imported beef, pork, and chicken by high resolution gas chromatography/mass spectrometry (HR-GC/MS). Results of the monitoring for the last 5 years are presented. The levels of PCDD/Fs were similar to other studies except two unusually high concentrations in pork and beef. Excessive levels greater than the Korean provisional maximum residue limit of PCDD/Fs were found in a sample of pork imported from Chile and a sample of beef imported from U.S, and those products were rejected and returned. There was no obvious trend or differences with respect to time or origin of meat in this study.

브로콜리 추출물의 향장활성 효과

김대용ㆍ조석철ㆍ권혁선ㆍ김미경(Dae Young KimㆍSeok chul ChoㆍHyuk sun KwonㆍMee Kyung Kim) 한국인체미용예술학회 2016 한국인체미용예술학회지 Vol.17 No.1

In this study, we investigated the anti-oxidative effects, antimicrobial, and anti-inflammatory activities of the extracts from broccoli to evaluate its use as a functional ingredient in cosmetics. The extracts are WE (water extract at RT), MWE (mineral water extract at RT), EE (70% ethanol extract at RT), and USEE (heat process for 12 hours at 60℃ after ultrasonification for six hours with 70% ethanol). The measurements of the total polyphenol content from broccoli extracts were highest in EE at 425.24±13.9 mg/g dry. The result of electron donating ability of EE was the highest at 10,000 ㎍/㎖ as 97.81% and increased in a concentration-dependent manner. The ferric reducing antioxidant powder (FRAP) of EE was the highest at 10,000 ㎍/㎖ as 1235.6 μM. In an antimicrobial activity test, the EE showed significant antimicrobial activity against S. epidermidis, S. aureus, E. coli, P. acnes, and P. ovale. Furthermore, broccoli extract exhibited no cytotoxicity in RAW 264.7 cells. Also anti- inflammatory activity by NO assay showed LPS-induced NO was significantly inhibited following treatment with EE of 1,000 ㎍/㎖. Therefore, the broccoli ethanol extract can be used as a resource of natural cosmetic material for functional cosmetics.

천연자원으로부터 아실코에이: 콜레스테롤아실 전달효소 저해제의 탐색

김미경,권병목,배기환,최돈하,이학주,김홍은,김영국,Kim, Mi-Kyung,Kwon, Byoung-Mog,Bae, Ki-Hwan,Choi, Don-Ha,Lee, Hak-Ju,Kim, Hong-Eun,Kim, Young-Kook 한국생약학회 1999 생약학회지 Vol.30 No.4

Acyl-CoA: Cholesterol Acyltransferase (ACAT) is a key enzyme responsible for cholesteryl ester formation in atherogenesis and in cholesterol absorption from the intestines. In addition under pathological conditions, formation and accumulation of cholesteryl ester as lipid droplets by ACAT within macrophages constitute a characteristic feature of early lesions of atherosclerotic plaques. ACAT inhibitors are expected to be effective for treatment of atherosclerosis and hypercholesterolemia. ACAT inhibitors of natural origin have been rarely reported. In our screening program for ACAT inhibitors, 303 plants were extracted with methanol or ethanol, and screened for the inhibitory activity against ACAT from the rat liver microsome. Extracts of 13 plants including Quercus aliena, Diospyros kaki, Platycarya strobilacea and Hibiscus syriacus inhibited more than 90% of ACAT activity and 43 samples in alcohol extracts such as Magnolia obovata and Panax ginseng also inhibited more than 70% of ACAT activity at a concentration of $100\;{\mu}g/ml$.

LANDSAT 영상을 이용한 세종특별자치시의 도시화와 열섬현상 분석

김미경,김상필,김남훈,손홍규,Kim, Mi-Kyeong,Kim, Sang-Pil,Kim, Nam-Hoon,Sohn, Hong-Gyoo 대한토목학회 2014 대한토목학회논문집 Vol.34 No.3

우리나라는 세계적으로 유례없이 도시화가 급속하게 이루어졌으며 그 결과, 국토 면적의 10%인 수도권에 인구의 50%가 밀집하여 국토 개발의 불균형 및 열섬현상을 비롯한 각종 도시 문제가 수도권을 중심으로 발생하고 있다. 이에 수도권의 과잉 밀집을 해소하고 국토의 균형개발을 위해 2012년 7월 세종특별자치시가 출범하였으며, 세종시는 2000년대 후반부터 실질적인 개발이 진행되어 급격한 도시화를 겪고 있는 곳으로 급격한 개발에 따른 도시 문제를 수반할 것으로 예상된다. 도시화로 인한 지표면 특성의 변화는 도시열섬의 주요인이며 도시열섬현상은 결과적으로 도시지역의 기후변화 및 자연환경에 영향을 끼칠 수 있다. 본 논문은 급속한 도시화를 겪고 있는 세종시를 대상으로 도시화의 정도와 도시열섬효과를 파악하였고 도시화와 지표면온도와의 상관관계를 분석하였다. 이를 위하여 LANDSAT 영상을 사용하였으며, 2001년부터 2013년까지의 LANDSAT의 $0.63{\mu}m-1.75{\mu}m$에 해당하는 적색, 근적외선, 중적외선 영역 영상으로 NDVI, NDBI, UI를 산출하였고, $10.4{\mu}m-12.5{\mu}m$에 해당하는 열적외선 영역대의 영상을 통하여 세종시의 지표온도를 추정하였다. 산출된 각 지수와 지표면 온도를 바탕으로 세종시의 NDVI의 변화, UI의 변화를 파악하였고, TVI를 통해 도시열섬을 분석하였다. TVI를 통해 새로 건설된 행정중심복합도시 지역 일대를 중심으로 도시열섬현상이 두드러지게 증가한 것을 확인할 수 있었고 각 지수와 지표온도와의 상관관계 분석 결과 NDVI가 높을수록 온도는 낮아지는 음의 상관 관계를, NDBI와 UI는 증가할수록 온도가 높아지는 양의 상관관계를 갖는 것으로 나타났으며 이 중에서도 UI는 지표면 온도와 가장 강한 양의 상관관계를 보였다. Rapid urbanization of Korea was an unprecedented example in the world and urban population increased significantly. As a result, unbalanced distribution of population is serious problem in Korea because approximately 50% of the population is concentrated in the capital area that is 10% of nation's territory, thereby occurring various urban problems including UHI. Hence, Sejong Special Autonomous City was inaugurated officially on 2 July 2012 in order to decentralize population of capital area and induce more balanced regional development. The Sejong City has been changed drastically over a period of years as developed practically since the late 2000's and is expected to have new problems of urbanization. The land cover change due to urbanization is the main cause of UHI that urban area is significantly warmer than its surrounding areas and UHI is not only affecting urban climate change but also natural environment. So the purpose of this research is to analyze level of urbanization and UHI effect and to provide the correlation analysis between Land Surface Temperature and spectral indices. To achieve this, satellite imagery from LANDSAT were used. NDVI, NDBI, and UI were calculated using red, near-infrared, mid-infrared ($0.63{\mu}m-1.75{\mu}m$) images and LST was retrieved utilizing thermal infrared ($10.4{\mu}m-12.5{\mu}m$) image. Based on each index and LST, Changes of NDVI, UI and UHI through TVI were analyzed in Sejong City. UHI effect increased around newly constructed multi-functional administrative city, the correlation between LST and NDVI was negative and UI was strong positive.

당뇨병성 방광병증으로 진단된 배뇨장애 환자 치험 1례

김미경,김보람,김용형,최동준,한창호,정승현,신길조,이원철,Kim, Mi-Kyung,Kim, Bo-Ram,Kim, Yong-Hyung,Choi, Dong-Jun,Han, Chang-Ho,Jung, Seung-Hyun,Shin, Gil-Jo,Lee, Won-Chul 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.4

There's a case of a 74-year-old male patient who was recommended urethral catheterization because of voiding problem which is diagnosed as diabetic cystopathy. According to the Differentiation of Symptoms(辨證), we classified his situation as kidney yang deficiency (腎陽虛衰), prescribed Uchasingi-hwan(牛車腎氣丸). and treated with moxibustion and electroacupuncture. After nine days of such treatments, he started voiding himself without catheterization, and the volume of residual urine decreased. So we report this case.

우리나라 폐사 야생조류에서의 농약 분석

김미경,윤선종,김동규,봉영훈,김희진,장정희,정갑수,Kim, MeeKyung,Yun, Seon Jong,Kim, Dong-Gyu,Bong, Young-Hoon,Kim, Heuijin,Jang, Jung-Hee,Chung, Gab-Soo 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.2

Pesticides are extensively used for the control of crop pests in agriculture and forestry. Organophosphate (OP) and carbamate pesticides are especially effective for the control of a variety of harmful insects. However, these cholinesterase inhibitors are also dangerous to non-target organisms (wildlife and other animals) because of their high acute toxicity. Most poisonings by pesticides occur as a result of misuse or accidental exposure, but intentional killing of unwanted animals also occurs. At the request of a local autonomous entity, we investigated wild bird poisonings by pesticides from 2003 to 2007. The 207 suspicious samples of pesticide poisoning based on the necropsy were analyzed by GC/NPD, GC/FPD, or GC/MSD. We looked for trends in the identification of pesticides in wild birds thought to have died from poisoning. Pesticides were determined in 59% of the total samples analyzed. Phosphamidon and monochrotophos were the most common pesticides identified, which amounted to 77% of the subtotal. Other OP and carbamate pesticides were also found in various concentrations from dead wild birds. The determined rates of pesticides were as high as 86% and 76% in 2003 and 2006, respectively, during an outbreak of avian influenza in Korea.

부모의 사회경제적 수준이 도시와 농촌 중고등학생의 영양소 섭취에 미치는 영향

김미경,기모란,방금녀,김기량,최보율,권영준,이상선,김찬,강윤주,Kim, Mi-Kyung,Ki, Mo-Ran,Bang, Kum-Nyu,Kim, Ki-Rang,Choi, Bo-Youl,Kwon, Young-Jun,Lee, Sang-Sun,Kim, Chan,Kang, Yun-Ju 대한지역사회영양학회 1998 대한지역사회영양학회지 Vol.3 No.4

This study was conducted to assess the nutrient intake patterns among urban and rural adolescents and to investigate the effects due to parent's socioeconomic status and other factors, such as mother's job, family type and regular exercise on that pattern. 2,455 middle and high school students living in Seoul and Yangpong, Kyounffi-Do participated in a self-administered questionnaire that was used to collect data. The one-day dietary intake was surveyed through a 24-hour recall method. The factors significantly different between urban and rural adolescents according to monthly income, parent's education level, mother's job, family type and exercise. Income, the parents' education level and regular exercise were associated with the patterns of nutrient intakes as a percent of the RDA. So, when adjusted for parental income, the father's and mother's education level and regular exercise, there were no signifcant differences within the patterns of nutritional intake between urban and rural adolescents. The results provided the information regarding the determinants of nutrient status among adolescents and were expected to be helpful for planning school health promotion programs.

어린이집 조직문화가 보육교사의 배려적사고와 교사효능감에 미치는 영향

김미경 ( Kim Mi Kyung ),김혜순 ( Kim Hye Soon ) 동국대학교 교육연구소 2018 교육연구 Vol.3 No.2

본 연구에서는 어린이집 조직문화가 보육교사의 배려적사고와 교사효능감에 미치는 영향을 알아보고자 한다. 이를 위해 연구대상은 경북 G시에 소재해 있는 국·공립, 법인, 직장 및 가정 어린이집 교사 228명이었다. 조직문화를 측정하기 위해 송지혜(2013)의 척도, 배려적 사고를 측정하기 위해 김수동, 안재진, 이정연(2014)의 척도 그리고 교사효능감을 측정하기 위해 김명란(2016)의 척도가 사용되었다. 수집된 자료의 분석은 SPSS/WIN 20.0 프로그램을 이용하여 신뢰도분석, 빈도분석, 기술통계분석, t-test, ANOVA, 상관분석, 위계적 회귀분석이 이루어졌다. 연구 결과는 다음과 같다. 첫째, 관계문화가 높게 지각되었고, 배려적 사고는 보통 수준을 조금 상회하였고, 개인적 교사효능감이 일반적 교사효능감에 비해 다소 높은 것으로 나타났다. 둘째, 어린이집 조직문화와 교사의 배려적사고 및 교사효능감 간의 관계에서는 모두가 유의미한 정적 상관을 보였다. 셋째, 보육교사의 학력이 높을수록 배려적사고가 높고, 근무하고 있는 어린이집의 설립 기간이 짧은 기관의 보육교사가 배려적사고가 높았다. 또한 어린이집의 조직문화 중에서는 관계문화와 합리문화가 보육교사의 배려적사고에 유의미한 정의 영향을 미치는 것으로 나타났다. 넷째, 기혼, 보육교사의 경력이 높을수록 교사의 교사효능감에 미치는 상대적 영향력이 큰 것으로 예측되었다. 결론적으로 어린이집이 관계적이고 합리적인 조직문화가 잘 구축되어 있을수록 교사의 배려적사고와 교사효능감에 긍정적인 영향을 미친다고 볼 수 있다. In this study, we look at the effects of the organization culture of daycare centers on the caring thinking and the effectiveness of teachers. Specific research issues for this purpose are as follows. Research 1. The trend and general background of child care organization culture, child care teacher consideration, teacher efficiency awareness (age, academic background, type of work organization, experience, age of infant, degree of progress, marriage status) Research 2. What is the correlation between daycare center organization culture, child care teacher consideration, and teacher efficiency? Research 3. How does the organization culture of daycare centers affect the consideration and effectiveness of child care teachers ? The methods of this study are as follows. It was done through a survey. The survey included 79 teachers from state, public, corporate, working and children's homes in G City, North Gyeongsang Province, and 149 teachers from private and private schools. Total 228 teachers participated. The researcher used research scale from organization culture by Song-Jihye(2013), child care teacher consideration by Kim-Sudong, An-Jaejin, Lee-jeongyoen(2014) and teacher efficacy by Kim-Myeongran(2016) For the analysis of the collected data, It's calculated Cronbach’ α by using SPSS/WIN 20.0 program, which shows the internal consistency. In order to examine the general background of childcare teachers, frequency analysis was conducted and a t-test and one-way ANOVA were performed to see if there was a difference in the level of variables. The mean and standard deviation of the main variables were calculated , Correlation analysis, and hierarchical regression analysis. The results of the study are as follows. First, the tendency of major variants was high in the culture of relationships, consideration was slightly higher than normal, and personal teacher efficiency was slightly higher than general teacher efficacy. Consideration showed a significant difference depending on age, academic background, experience, length of institution establishment, and marital status, and teacher efficacy varied depending on age, experience, and marital status. Second, the relationship between the organizational culture of daycare centers and the caring thinking of child care teachers, the relationship between child care organization culture and teacher efficacy, and the caring thinking of child care teachers and the teacher's sense of efficiency showed significant static. Third, among background changes of child care teachers, educational background can have a significant effect on the consideration of children, and the period of institution establishment is shown to have a significant influence on the value of care, so the educational background of child teachers is more important. Among the organizational cultures of daycare centers, it was found that the culture of relationship and rationality had a significant influence on the consideration of child care teachers, and that the culture of rationality had a greater influence on the consideration of child care teachers. Among the background changes of child care teachers, the more important the difference in experience and marital status is in the sense of teacher efficiency, the more likely it is for a child teacher to be a single teacher, and the more likely it is for a teacher to be married. Among the organizational cultures of daycare centers, it was found that the culture of relationship and rationality had a significant effect on the teacher's sense of efficacy, and that the culture of relation had a greater influence on the relative effect of the teacher's ability. Therefore, the more relevant and reasonable organizational culture in daycare centers is well established, the more positive the effect is on the consideration and effectiveness of child care teachers.

공배양의 작용기전에 관한 연구

김미경,주보선,김미선,문화숙,이규섭,김한도,Kim, Mi-Kyoung,Joo, Bo-Sun,Kim, Mi-Sun,Moon, Hwa-Sook,Lee, Kyu-Sup,Kim, Han-Do 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.1

Objective: A number of studies to improve in vitro culture conditions have been tried over past ten years by using co-culture system with helper somatic cells. However, the mechanism of coculture is poorly understood. This study was designed to understand the mechanism for the mode of actual action of co-culture using co-culture system of ICR strain's 1-cell embryos with human oviduct epithelial cells by examining the effect of conditioned medium and contactless coculture using a cell culture insert on the embryo development and by measuring the level of superoxide anion from conditioned medium after co-culture. Methods: ICR strain's zygote embryos were cultured in medium alone (control), coculture, conditioned medium, or contactless coculture system for 6 days. Conditioned media (CM) were prepared as following 5 groups. All CM were collected after culturing oviduct cells for 2 days. CM-1 was stored at $-20^{\circ}C$ until use, and CM-2 was prepared just before use as a culture medium. CM-3 was cocultured with embryos and retrieved just before use. CM-4 and CM-5 were derives from the microfilteration of CM-2 and CM-3, respectively, using Microcon-10 (10 kDa molecular weight cut-off). The percentage of the embryos developed to hatched blastocyst stage and the level of superoxide anion in supernatant from medium alone culture (control), coculture, and contactless coculture were measured. Results: The rates of embryo development to the hatched blastocyst stage were significantly higher in coculture (43%) than in control (0%) (p<0.05). The CM-1 group had no embryo development since 2-cell embryonic stage, whereas the CM-2, CM-3, CM-4 and CM-5 groups had the improved development to 4 or 8 cell embryo stage, but the similar rate of development to hatched blastocyst compared to control. The effect of coculture on embryo development was disappeared in the contactless coculture group. The level of superoxide anion was significantly reduced in coculture group compared to control. Conclusion: It is concluded that the present coculture system overcomes the 2-cell block in vitro and improves the embryo development. This beneficial effect may be due to the direct cell-cell contact between embryo and helper cells or the removal of deleterious components from medium rather than the embryotrophic factors.